Restriction map for
pFN21AB8177
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII AccII AccIII AciI AclI
AcyI AflII AgeI AhdI AocI
Aor51HI ApaI AscI Asp700I AspEI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmFI BsoBI BspDI
BspEI BspHI BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoT22I FauI
FseI FspI HaeII Hin1I HindIII
HpaI KasI KspI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI TaqI TthHB8I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 486 GGTACC G^GTACC
AccI 1376 GTCTAC GT^[AC][GT]AC
AflIII 557 ACATGT A^C[GA][CT]GT
AseI 544 ATTAAT AT^TAAT
AsnI 544 ATTAAT AT^TAAT
Asp718I 486 GGTACC G^GTACC
AspI 348 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 486 GGTACC G^G[CT][GA]CC
BglII 875 AGATCT A^GATCT
BsaBI 925 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 251 GAATGC GAATGC(1/-1)
BspLU11I 557 ACATGT A^CATGT
BspMI 525 ACCTGC ACCTGC(4/8)
CfoI 758 GCGC GCG^C
DraII 1255 AGGACCT [GA]G^G[ACGT]CC[CT]
EarI 1593 GAAGAG CTCTTC(1/4)
EcoNI 1011 CCTCCATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1255 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 501 GAATTC G^AATTC
EcoRV 260 GATATC GAT^ATC
HgaI 1374 GCGTC GACGC(5/10)
HhaI 758 GCGC GCG^C
HinP1I 758 GCGC G^CGC
HincII 1516 GTCAAC GT[CT]^[GA]AC
HindII 1516 GTCAAC GT[CT]^[GA]AC
KpnI 486 GGTACC GGTAC^C
Ksp632I 1593 GAAGAG CTCTTC(1/4)
MamI 925 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI 557 ACATGT [GA]CATG^[CT]
PflFI 348 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 685 CCAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1255 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 544 ATTAAT AT^TAAT
SfcI 662 CTACAG C^T[GA][CT]AG
SmlI 5 CTCAAG C^T[CT][GA]AG
SspI 1092 AATATT AAT^ATT
Tth111I 348 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 685 CCAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Alw44I 199 GTGCAC G^TGCAC
1584 GTGCAC
AlwNI 506 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
1475 CAGCTCCTG
ApaLI 199 GTGCAC G^TGCAC
1584 GTGCAC
AvaII 777 GGACC G^G[AT]CC
1256 GGACC
BanII 634 GAGCCC G[GA]GC[CT]^C
676 GAGCCC
BbsI 1141 GAAGAC GAAGAC(2/6)
864 GTCTTC
BclI 924 TGATCA T^GATCA
1469 TGATCA
BcnI 240 CCGGG CC^[GC]GG
266 CCCGG
BpuAI 1141 GAAGAC GAAGAC(2/6)
864 GTCTTC
BsgI 234 GTGCAG GTGCAG(16/14)
1433 GTGCAG
Bsp1407I 293 TGTACA T^GTACA
468 TGTACA
BsrDI 1410 CATTGC GCAATG(2/0)
1150 GCAATG
BsrFI 91 ACCGGC [GA]^CCGG[CT]
380 ACCGGC
BsrGI 293 TGTACA T^GTACA
468 TGTACA
Cfr10I 91 ACCGGC [GA]^CCGG[CT]
380 ACCGGC
DsaI 395 CCATGG C^C[GA][CT]GG
413 CCATGG
FbaI 924 TGATCA T^GATCA
1469 TGATCA
MseI 17 TTAA T^TAA
545 TTAA
MspA1I 356 CAGCTG C[AC]G^C[GT]G
1289 CAGCTG
NciI 240 CCGGG CC^[GC]GG
266 CCCGG
NcoI 395 CCATGG C^CATGG
413 CCATGG
PvuII 356 CAGCTG CAG^CTG
1289 CAGCTG
SexAI 182 ACCTGGT A^CC[AT]GGT
563 ACCTGGT
SspBI 293 TGTACA T^GTACA
468 TGTACA
TfiI 722 GATTC G^A[AT]TC
1155 GATTC
Tru9I 17 TTAA T^TAA
545 TTAA
XcmI 1037 CCACAGCCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1556 CCATCTTCCTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI BbvI BfaI BmyI BpmI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TseI Tsp45I
Tsp509I TspRI XhoII