(Circular) (Six-base) MAPSORT of: pFN21AB8177.seq Check: 9413 from: 1 to: 6307 pFN21AB8177 6307 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2539 2539 Size: 6307 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4117 278 Size: 53 83 186 936 2581 2468 Fragments arranged by size: 2581 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3382 3650 1937 Size: 1445 268 4594 Fragments arranged by size: 4594 1445 268 Acc65I G'GTAC_C Cuts at: 2491 3628 2491 Size: 1137 5170 AclI AA'CG_TT Cuts at: 1563 4434 4807 1563 Size: 2871 373 3063 Fragments arranged by size: 3063 2871 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2451 2869 4370 5754 6022 1632 Size: 819 418 1501 1384 268 1917 Fragments arranged by size: 1917 1501 1384 819 418 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6110 AflIII A'CryG_T Cuts at: 1203 2562 5206 6183 1203 Size: 1359 2644 977 1327 Fragments arranged by size: 2644 1359 1327 977 AgeI A'CCGG_T Cuts at: 5138 5138 Size: 6307 AhdI GACnn_n'nnGTC Cuts at: 5036 5036 Size: 6307 AleI CACnn'nnGTG Cuts at: 1166 2211 2447 2742 1166 Size: 1045 236 295 4731 Fragments arranged by size: 4731 1045 295 236 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6307 AlwNI CAG_nnn'CTG Cuts at: 2516 3485 4050 5622 2516 Size: 969 565 1572 3201 Fragments arranged by size: 3201 1572 969 565 ApaLI G'TGCA_C Cuts at: 2204 3589 4364 5520 2204 Size: 1385 775 1156 2991 Fragments arranged by size: 2991 1385 1156 775 ApoI r'AATT_y Cuts at: 1493 2506 3208 3228 3616 3842 3994 6103 1493 Size: 1013 702 20 388 226 152 2109 1697 Fragments arranged by size: 2109 1697 1013 702 388 226 152 20 AseI AT'TA_AT Cuts at: 160 1032 2550 4861 160 Size: 872 1518 2311 1606 Fragments arranged by size: 2311 1606 1518 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6307 AvaI C'yCGr_G Cuts at: 1870 1943 3632 1870 Size: 73 1689 4545 Fragments arranged by size: 4545 1689 73 BamHI G'GATC_C Cuts at: 3637 4004 3637 Size: 367 5940 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2491 3628 5083 618 Size: 325 217 627 704 1137 1455 1842 Fragments arranged by size: 1842 1455 1137 704 627 325 217 BanII G_rGCy'C Cuts at: 1391 2643 2685 3626 1391 Size: 1252 42 941 4072 Fragments arranged by size: 4072 1252 941 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2862 3153 928 Size: 971 963 291 4082 Fragments arranged by size: 4082 971 963 291 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4498 4532 1903 Size: 34 2561 34 3678 Fragments arranged by size: 3678 2561 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4199 5409 4199 Size: 1210 5097 BclI T'GATC_A Cuts at: 1548 2929 3474 1548 Size: 1381 545 4381 Fragments arranged by size: 4381 1381 545 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4918 136 Size: 107 122 71 631 605 3246 1525 Fragments arranged by size: 3246 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2880 6302 2880 Size: 3422 2885 BlpI GC'TnA_GC Cuts at: 3724 3724 Size: 6307 Bme1580I G_kGCm'C Cuts at: 1790 2208 3593 4368 5524 1790 Size: 418 1385 775 1156 2573 Fragments arranged by size: 2573 1385 1156 775 418 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6307 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2230 4038 4996 Size: 416 53 83 399 24 813 1808 958 Cuts at: 4996 442 Size: 1753 Fragments arranged by size: 1808 1753 958 813 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6307 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2331 3074 3206 3593 4967 1239 Size: 1092 743 132 387 1374 2579 Fragments arranged by size: 2579 1374 1092 743 387 132 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1995 4432 5297 5595 1995 Size: 2437 865 298 2707 Fragments arranged by size: 2707 2437 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2390 2647 3087 3497 4970 882 Size: 617 891 257 440 410 1473 2219 Fragments arranged by size: 2219 1473 891 617 440 410 257 BsaAI yAC'GTr Cuts at: 493 1204 4098 493 Size: 711 2894 2702 Fragments arranged by size: 2894 2702 711 BsaBI GATnn'nnATC Cuts at: 2934 6301 2934 Size: 3367 2940 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4114 4496 5131 275 Size: 53 83 186 936 2581 382 635 1451 Fragments arranged by size: 2581 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4740 5138 5412 5559 5970 4740 Size: 398 274 147 411 5077 Fragments arranged by size: 5077 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2049 2079 2321 2351 1178 Size: 30 165 30 646 30 242 30 5134 Fragments arranged by size: 5134 646 242 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2342 2623 3482 1186 Size: 1156 281 859 4011 Fragments arranged by size: 4011 1156 859 281 BseYI C'CCAG_C Cuts at: 2633 2859 5510 6006 2633 Size: 226 2651 496 2934 Fragments arranged by size: 2934 2651 496 226 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2260 3459 1163 Size: 573 524 1199 4011 Fragments arranged by size: 4011 1199 573 524 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3757 4518 5546 1540 Size: 275 186 1756 761 1028 2301 Fragments arranged by size: 2301 1756 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2208 2851 3593 4368 4453 5524 2208 Size: 643 742 775 85 1071 2991 Fragments arranged by size: 2991 1071 775 742 643 85 BsmI GAATG_Cn' Cuts at: 2262 3818 3911 2262 Size: 1556 93 4658 Fragments arranged by size: 4658 1556 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2208 2643 2685 2851 3593 3626 4368 Size: 399 418 435 42 166 742 33 742 Cuts at: 4368 4453 5524 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 742 435 418 399 166 85 42 33 BspEI T'CCGG_A Cuts at: 5970 5970 Size: 6307 BspHI T'CATG_A Cuts at: 4196 5926 4196 Size: 1730 4577 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2539 3662 844 Size: 1695 1123 3489 Fragments arranged by size: 3489 1695 1123 BsrBI CCG'CTC Cuts at: 1394 1823 4194 1394 Size: 429 2371 3507 Fragments arranged by size: 3507 2371 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3162 3414 4802 4976 65 Size: 1183 1914 252 1388 174 1396 Fragments arranged by size: 1914 1396 1388 1183 252 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2096 2385 4951 5138 1700 Size: 110 286 289 2566 187 2869 Fragments arranged by size: 2869 2566 289 286 187 110 BsrGI T'GTAC_A Cuts at: 96 2298 2473 96 Size: 2202 175 3930 Fragments arranged by size: 3930 2202 175 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6307 BssSI C'ACGA_G Cuts at: 4367 5379 4367 Size: 1012 5295 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6307 BstBI TT'CG_AA Cuts at: 5111 5111 Size: 6307 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6307 BstYI r'GATC_y Cuts at: 1972 2880 2888 3543 3637 4004 4389 4406 5858 Size: 908 8 655 94 367 385 17 1452 Cuts at: 5858 5944 5956 6302 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 908 655 385 367 346 94 86 17 12 8 BtgI C'CryG_G Cuts at: 513 1065 2400 2418 513 Size: 552 1335 18 4402 Fragments arranged by size: 4402 1335 552 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5568 Fragments arranged by size: 5568 683 56 BtsI GCAGTG_nn' Cuts at: 3823 4617 4637 3823 Size: 794 20 5493 Fragments arranged by size: 5493 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6307 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6272 DraI TTT'AAA Cuts at: 3611 3611 Size: 6307 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5314 809 Size: 507 3998 1802 Fragments arranged by size: 3998 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3754 4643 6272 Size: 54 1425 50 275 18 1924 889 1629 Cuts at: 6272 8 Size: 43 Fragments arranged by size: 1924 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3754 1537 Size: 275 1942 4090 Fragments arranged by size: 4090 1942 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3593 4237 1353 Size: 2240 644 3423 Fragments arranged by size: 3423 2240 644 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4894 5268 5414 387 Size: 1064 138 3305 374 146 1280 Fragments arranged by size: 3305 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2331 2451 2869 3074 3206 3593 4370 Size: 393 699 120 418 205 132 387 777 Cuts at: 4370 4967 5754 6022 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 777 699 597 418 393 387 268 205 132 120 EcoNI CCTnn'n_nnAGG Cuts at: 3020 3020 Size: 6307 EcoO109I rG'GnC_Cy Cuts at: 3261 3261 Size: 6307 EcoRI G'AATT_C Cuts at: 2506 3616 3994 2506 Size: 1110 378 4819 Fragments arranged by size: 4819 1110 378 EcoRV GAT'ATC Cuts at: 2267 2267 Size: 6307 FspI TGC'GCA Cuts at: 4813 4813 Size: 6307 HaeII r_GCGC'y Cuts at: 1678 5454 1678 Size: 3776 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3466 3498 4948 4980 5022 Size: 32 135 32 1894 32 1450 32 42 Cuts at: 5022 5054 1373 Size: 32 2626 Fragments arranged by size: 2626 1894 1450 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3523 3651 3897 669 Size: 988 281 1585 128 246 3079 Fragments arranged by size: 3079 1585 988 281 246 128 HindIII A'AGCT_T Cuts at: 748 748 Size: 6307 HpaI GTT'AAC Cuts at: 3897 3897 Size: 6307 KpnI G_GTAC'C Cuts at: 2495 3632 2495 Size: 1137 5170 MfeI C'AATT_G Cuts at: 3906 3906 Size: 6307 MluI A'CGCG_T Cuts at: 6183 6183 Size: 6307 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2203 2953 3510 5421 5605 1305 Size: 898 750 557 1911 184 2007 Fragments arranged by size: 2007 1911 898 750 557 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4485 Fragments arranged by size: 4485 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2211 2447 2742 4265 4624 4783 518 Size: 648 1045 236 295 1523 359 159 2042 Fragments arranged by size: 2042 1523 1045 648 359 295 236 159 MspA1I CmG'CkG Cuts at: 1606 2363 3296 3723 4400 5548 5793 1606 Size: 757 933 427 677 1148 245 2120 Fragments arranged by size: 2120 1148 933 757 677 427 245 NcoI C'CATG_G Cuts at: 513 1065 2400 2418 513 Size: 552 1335 18 4402 Fragments arranged by size: 4402 1335 552 18 NdeI CA'TA_TG Cuts at: 387 387 Size: 6307 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6307 NotI GC'GGCC_GC Cuts at: 3754 3754 Size: 6307 NspI r_CATG'y Cuts at: 1132 1229 2566 3665 5210 1132 Size: 97 1337 1099 1545 2229 Fragments arranged by size: 2229 1545 1337 1099 97 PasI CC'CwG_GG Cuts at: 1348 2516 1348 Size: 1168 5139 PciI A'CATG_T Cuts at: 2562 5206 2562 Size: 2644 3663 PflMI CCAn_nnn'nTGG Cuts at: 1108 2696 1108 Size: 1588 4719 PfoI T'CCnGG_A Cuts at: 3222 3257 3222 Size: 35 6272 PmeI GTTT'AAAC Cuts at: 3611 3611 Size: 6307 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4376 4408 5889 5921 4376 Size: 32 1481 32 4762 Fragments arranged by size: 4762 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3261 3261 Size: 6307 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6307 PsiI TTA'TAA Cuts at: 3877 3877 Size: 6307 PstI C_TGCA'G Cuts at: 830 3659 830 Size: 2829 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6307 PvuII CAG'CTG Cuts at: 2363 3296 2363 Size: 933 5374 SalI G'TCGA_C Cuts at: 1936 3649 1936 Size: 1713 4594 SbfI CC_TGCA'GG Cuts at: 3659 3659 Size: 6307 ScaI AGT'ACT Cuts at: 4555 6267 4555 Size: 1712 4595 SexAI A'CCwGG_T Cuts at: 2187 2568 2187 Size: 381 5926 SfcI C'TryA_G Cuts at: 826 1045 2667 3655 4790 5471 5662 826 Size: 219 1622 988 1135 681 191 1471 Fragments arranged by size: 1622 1471 1135 988 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6307 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6307 SmaI CCC'GGG Cuts at: 1872 3634 1872 Size: 1762 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2010 4411 5312 5574 820 Size: 197 926 67 2401 901 262 1553 Fragments arranged by size: 2401 1553 926 901 262 197 67 SnaBI TAC'GTA Cuts at: 493 4098 493 Size: 3605 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6307 SphI G_CATG'C Cuts at: 1132 3665 1132 Size: 2533 3774 SspI AAT'ATT Cuts at: 5 52 3099 4231 5 Size: 47 3047 1132 2081 Fragments arranged by size: 3047 2081 1132 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6307 StyI C'CwwG_G Cuts at: 513 1065 2400 2418 2643 3746 513 Size: 552 1335 18 225 1103 3074 Fragments arranged by size: 3074 1335 1103 552 225 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2873 4334 4351 4504 4689 5134 325 Size: 1255 1293 1461 17 153 185 445 1498 Fragments arranged by size: 1498 1461 1293 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2298 2473 4553 Size: 274 80 33 51 1443 321 175 2080 Cuts at: 4553 6265 96 Size: 1712 138 Fragments arranged by size: 2080 1712 1443 321 274 175 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6267 6299 6267 Size: 32 6275 Tth111I GACn'n_nGTC Cuts at: 1316 2356 1316 Size: 1040 5267 XbaI T'CTAG_A Cuts at: 3643 3643 Size: 6307 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3049 3568 3049 Size: 519 5788 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6307 XmaI C'CCGG_G Cuts at: 1870 3632 1870 Size: 1762 4545 XmnI GAAnn'nnTTC Cuts at: 4436 4436 Size: 6307 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4115 276 Size: 53 83 186 936 2581 2468 Fragments arranged by size: 2581 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI