Restriction map for
pFN21AB8177
(Linear) (Six-base) MAPSORT of: KIBB8177.seq Check: 5091 from: 1 to: 1602
KIBB8177.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 534 1602
Size: 534 1068
AccI GT'mk_AC
Cuts at: 0 1377 1602
Size: 1377 225
Acc65I G'GTAC_C
Cuts at: 0 486 1602
Size: 486 1116
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 864 1602
Size: 446 418 738
Fragments arranged by size:
738 446 418
AflIII A'CryG_T
Cuts at: 0 557 1602
Size: 557 1045
AleI CACnn'nnGTG
Cuts at: 0 206 442 737 1602
Size: 206 236 295 865
Fragments arranged by size:
865 295 236 206
AlwNI CAG_nnn'CTG
Cuts at: 0 511 1480 1602
Size: 511 969 122
Fragments arranged by size:
969 511 122
ApaLI G'TGCA_C
Cuts at: 0 199 1584 1602
Size: 199 1385 18
Fragments arranged by size:
1385 199 18
ApoI r'AATT_y
Cuts at: 0 501 1203 1223 1602
Size: 501 702 20 379
Fragments arranged by size:
702 501 379 20
AseI AT'TA_AT
Cuts at: 0 545 1602
Size: 545 1057
BanI G'GyrC_C
Cuts at: 0 486 1602
Size: 486 1116
BanII G_rGCy'C
Cuts at: 0 638 680 1602
Size: 638 42 922
Fragments arranged by size:
922 638 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 857 1148 1602
Size: 857 291 454
Fragments arranged by size:
857 454 291
BclI T'GATC_A
Cuts at: 0 924 1469 1602
Size: 924 545 133
Fragments arranged by size:
924 545 133
BglII A'GATC_T
Cuts at: 0 875 1602
Size: 875 727
Bme1580I G_kGCm'C
Cuts at: 0 203 1588 1602
Size: 203 1385 14
Fragments arranged by size:
1385 203 14
BmrI ACTGGGnnnn_n'
Cuts at: 0 225 1602
Size: 225 1377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 1069 1201 1588 1602
Size: 326 743 132 387 14
Fragments arranged by size:
743 387 326 132 14
BsaI GGTCTCn'nnnn_
Cuts at: 0 385 642 1082 1492 1602
Size: 385 257 440 410 110
Fragments arranged by size:
440 410 385 257 110
BsaBI GATnn'nnATC
Cuts at: 0 929 1602
Size: 929 673
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 44 74 316 346 1602
Size: 44 30 242 30 1256
Fragments arranged by size:
1256 242 44 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 337 618 1477 1602
Size: 337 281 859 125
Fragments arranged by size:
859 337 281 125
BseYI C'CCAG_C
Cuts at: 0 628 854 1602
Size: 628 226 748
Fragments arranged by size:
748 628 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 1454 1602
Size: 255 1199 148
Fragments arranged by size:
1199 255 148
BsiHKAI G_wGCw'C
Cuts at: 0 203 846 1588 1602
Size: 203 643 742 14
Fragments arranged by size:
742 643 203 14
BsmI GAATG_Cn'
Cuts at: 0 257 1602
Size: 257 1345
Bsp1286I G_dGCh'C
Cuts at: 0 203 638 680 846 1588 1602
Size: 203 435 42 166 742 14
Fragments arranged by size:
742 435 203 166 42 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 534 1602
Size: 534 1068
BsrDI GCAATG_nn'
Cuts at: 0 1157 1409 1602
Size: 1157 252 193
Fragments arranged by size:
1157 252 193
BsrFI r'CCGG_y
Cuts at: 0 91 380 1602
Size: 91 289 1222
Fragments arranged by size:
1222 289 91
BsrGI T'GTAC_A
Cuts at: 0 293 468 1602
Size: 293 175 1134
Fragments arranged by size:
1134 293 175
BstYI r'GATC_y
Cuts at: 0 875 883 1538 1602
Size: 875 8 655 64
Fragments arranged by size:
875 655 64 8
BtgI C'CryG_G
Cuts at: 0 395 413 1602
Size: 395 18 1189
Fragments arranged by size:
1189 395 18
EarI CTCTTCn'nnn_
Cuts at: 0 1588 1602
Size: 1588 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 446 864 1069 1201 1588 1602
Size: 326 120 418 205 132 387 14
Fragments arranged by size:
418 387 326 205 132 120 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1015 1602
Size: 1015 587
EcoO109I rG'GnC_Cy
Cuts at: 0 1256 1602
Size: 1256 346
EcoRI G'AATT_C
Cuts at: 0 501 1602
Size: 501 1101
EcoRV GAT'ATC
Cuts at: 0 262 1602
Size: 262 1340
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1461 1493 1602
Size: 1461 32 109
Fragments arranged by size:
1461 109 32
HincII GTy'rAC
Cuts at: 0 1518 1602
Size: 1518 84
KpnI G_GTAC'C
Cuts at: 0 490 1602
Size: 490 1112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 948 1505 1602
Size: 198 750 557 97
Fragments arranged by size:
750 557 198 97
MslI CAynn'nnrTG
Cuts at: 0 206 442 737 1602
Size: 206 236 295 865
Fragments arranged by size:
865 295 236 206
MspA1I CmG'CkG
Cuts at: 0 358 1291 1602
Size: 358 933 311
Fragments arranged by size:
933 358 311
NcoI C'CATG_G
Cuts at: 0 395 413 1602
Size: 395 18 1189
Fragments arranged by size:
1189 395 18
NspI r_CATG'y
Cuts at: 0 561 1602
Size: 561 1041
PasI CC'CwG_GG
Cuts at: 0 511 1602
Size: 511 1091
PciI A'CATG_T
Cuts at: 0 557 1602
Size: 557 1045
PflMI CCAn_nnn'nTGG
Cuts at: 0 691 1602
Size: 691 911
PfoI T'CCnGG_A
Cuts at: 0 1217 1252 1602
Size: 1217 35 350
Fragments arranged by size:
1217 350 35
PpuMI rG'GwC_Cy
Cuts at: 0 1256 1602
Size: 1256 346
PvuII CAG'CTG
Cuts at: 0 358 1291 1602
Size: 358 933 311
Fragments arranged by size:
933 358 311
SexAI A'CCwGG_T
Cuts at: 0 182 563 1602
Size: 182 381 1039
Fragments arranged by size:
1039 381 182
SfcI C'TryA_G
Cuts at: 0 662 1602
Size: 662 940
SmlI C'TyrA_G
Cuts at: 0 5 1602
Size: 5 1597
SspI AAT'ATT
Cuts at: 0 1094 1602
Size: 1094 508
StyI C'CwwG_G
Cuts at: 0 395 413 638 1602
Size: 395 18 225 964
Fragments arranged by size:
964 395 225 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 868 1602
Size: 868 734
TatI w'GTAC_w
Cuts at: 0 293 468 1602
Size: 293 175 1134
Fragments arranged by size:
1134 293 175
Tth111I GACn'n_nGTC
Cuts at: 0 351 1602
Size: 351 1251
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1044 1563 1602
Size: 1044 519 39
Fragments arranged by size:
1044 519 39
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AlwNI ApaLI
ApoI AseI BanI BanII BbsI BclI BglII Bme1580I
BmrI BpmI BsaI BsaBI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BstYI
BtgI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I
HincII KpnI MmeI MslI MspA1I NcoI NspI PasI
PciI PflMI PfoI PpuMI PvuII SexAI SfcI SmlI
SspI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AsiSI AvaI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BfrBI BglI BlpI BmgBI BmtI
BplI Bpu10I BpuEI BsaAI BsaHI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EciI EcoICRI FalI
FseI FspI FspAI HaeII HindIII HpaI KasI MfeI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TstI XbaI XhoI XmaI XmnI ZraI