(Circular) (Six-base) MAPSORT of: pFN21AB6406.seq Check: 6734 from: 1 to: 5710 pFN21AB6406 5710 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3520 278 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3053 1937 Size: 1116 4594 Acc65I G'GTAC_C Cuts at: 3031 3031 Size: 5710 AclI AA'CG_TT Cuts at: 1563 3837 4210 1563 Size: 2274 373 3063 Fragments arranged by size: 3063 2274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2196 2592 3773 5157 5425 1632 Size: 564 396 1181 1384 268 1917 Fragments arranged by size: 1917 1384 1181 564 396 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5513 AflIII A'CryG_T Cuts at: 1203 4609 5586 1203 Size: 3406 977 1327 Fragments arranged by size: 3406 1327 977 AgeI A'CCGG_T Cuts at: 4541 4541 Size: 5710 AhdI GACnn_n'nnGTC Cuts at: 4439 4439 Size: 5710 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5710 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5710 AlwNI CAG_nnn'CTG Cuts at: 2135 3453 5025 2135 Size: 1318 1572 2820 Fragments arranged by size: 2820 1572 1318 ApaLI G'TGCA_C Cuts at: 3767 4923 3767 Size: 1156 4554 ApoI r'AATT_y Cuts at: 1493 2451 3019 3245 3397 5506 1493 Size: 958 568 226 152 2109 1697 Fragments arranged by size: 2109 1697 958 568 226 152 AseI AT'TA_AT Cuts at: 160 1032 4264 160 Size: 872 3232 1606 Fragments arranged by size: 3232 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 AvaI C'yCGr_G Cuts at: 1870 1943 2307 3035 1870 Size: 73 364 728 4545 Fragments arranged by size: 4545 728 364 73 BamHI G'GATC_C Cuts at: 3040 3407 3040 Size: 367 5343 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2020 3031 4486 618 Size: 325 217 627 233 1011 1455 1842 Fragments arranged by size: 1842 1455 1011 627 325 233 217 BanII G_rGCy'C Cuts at: 1391 2857 3029 1391 Size: 1466 172 4072 Fragments arranged by size: 4072 1466 172 BbeI G_GCGC'C Cuts at: 2024 2024 Size: 5710 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4739 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3901 3935 1903 Size: 34 1964 34 3678 Fragments arranged by size: 3678 1964 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3602 4812 3602 Size: 1210 4500 BclI T'GATC_A Cuts at: 1548 1548 Size: 5710 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4321 136 Size: 107 122 71 631 605 2649 1525 Fragments arranged by size: 2649 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2374 5705 2374 Size: 3331 2379 BlpI GC'TnA_GC Cuts at: 3127 3127 Size: 5710 Bme1580I G_kGCm'C Cuts at: 1790 3771 4927 1790 Size: 1981 1156 2573 Fragments arranged by size: 2573 1981 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5710 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2724 2832 3441 Size: 416 53 83 399 24 1307 108 609 Cuts at: 3441 4399 442 Size: 958 1753 Fragments arranged by size: 1753 1307 958 609 416 399 108 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2214 2937 4370 1239 Size: 975 723 1433 2579 Fragments arranged by size: 2579 1433 975 723 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3835 4700 4998 3835 Size: 865 298 4547 Fragments arranged by size: 4547 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4373 882 Size: 617 2874 2219 Fragments arranged by size: 2874 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2252 3501 493 Size: 711 1048 1249 2702 Fragments arranged by size: 2702 1249 1048 711 BsaBI GATnn'nnATC Cuts at: 5704 5704 Size: 5710 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2021 3517 3899 4534 Size: 53 83 186 936 488 1496 382 635 Cuts at: 4534 275 Size: 1451 Fragments arranged by size: 1496 1451 936 635 488 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3000 4143 4541 4815 4962 5373 3000 Size: 1143 398 274 147 411 3337 Fragments arranged by size: 3337 1143 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5485 Fragments arranged by size: 5485 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5710 BseYI C'CCAG_C Cuts at: 2597 4913 5409 2597 Size: 2316 496 2898 Fragments arranged by size: 2898 2316 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2577 1163 Size: 573 841 4296 Fragments arranged by size: 4296 841 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3160 3921 4949 1540 Size: 275 186 1159 761 1028 2301 Fragments arranged by size: 2301 1159 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3771 3856 4927 3771 Size: 85 1071 4554 Fragments arranged by size: 4554 1071 85 BsmI GAATG_Cn' Cuts at: 3221 3314 3221 Size: 93 5617 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2857 3029 3771 3856 4927 1391 Size: 399 1067 172 742 85 1071 2174 Fragments arranged by size: 2174 1071 1067 742 399 172 85 BspEI T'CCGG_A Cuts at: 5373 5373 Size: 5710 BspHI T'CATG_A Cuts at: 3599 5329 3599 Size: 1730 3980 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3065 844 Size: 2221 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3597 1394 Size: 429 1774 3507 Fragments arranged by size: 3507 1774 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2523 4205 4379 65 Size: 1183 1275 1682 174 1396 Fragments arranged by size: 1682 1396 1275 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4354 4541 1700 Size: 110 2544 187 2869 Fragments arranged by size: 2869 2544 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5710 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5710 BssSI C'ACGA_G Cuts at: 2457 3770 4782 2457 Size: 1313 1012 3385 Fragments arranged by size: 3385 1313 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5710 BstBI TT'CG_AA Cuts at: 4514 4514 Size: 5710 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5710 BstYI r'GATC_y Cuts at: 1972 2374 3040 3407 3792 3809 5261 5347 5359 Size: 402 666 367 385 17 1452 86 12 Cuts at: 5359 5705 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 666 402 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5158 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4971 Fragments arranged by size: 4971 683 56 BtsI GCAGTG_nn' Cuts at: 3226 4020 4040 3226 Size: 794 20 4896 Fragments arranged by size: 4896 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5710 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5675 DraI TTT'AAA Cuts at: 3014 3014 Size: 5710 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4717 809 Size: 507 3401 1802 Fragments arranged by size: 3401 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3157 4046 5675 Size: 54 1425 50 275 18 1327 889 1629 Cuts at: 5675 8 Size: 43 Fragments arranged by size: 1629 1425 1327 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3157 1537 Size: 275 1345 4090 Fragments arranged by size: 4090 1345 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2457 2545 3640 1353 Size: 1104 88 1095 3423 Fragments arranged by size: 3423 1104 1095 88 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4297 4671 4817 387 Size: 1064 138 2708 374 146 1280 Fragments arranged by size: 2708 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2196 2214 2592 2937 3773 4370 5157 Size: 393 564 18 378 345 836 597 787 Cuts at: 5157 5425 1239 Size: 268 1524 Fragments arranged by size: 1524 836 787 597 564 393 378 345 268 18 EcoNI CCTnn'n_nnAGG Cuts at: 2196 2690 2867 2196 Size: 494 177 5039 Fragments arranged by size: 5039 494 177 EcoRI G'AATT_C Cuts at: 2451 3019 3397 2451 Size: 568 378 4764 Fragments arranged by size: 4764 568 378 EcoRV GAT'ATC Cuts at: 2773 2773 Size: 5710 FspI TGC'GCA Cuts at: 4216 4216 Size: 5710 HaeII r_GCGC'y Cuts at: 1678 2024 4857 1678 Size: 346 2833 2531 Fragments arranged by size: 2833 2531 346 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2672 2704 4351 4383 4425 Size: 32 135 32 1100 32 1647 32 42 Cuts at: 4425 4457 1373 Size: 32 2626 Fragments arranged by size: 2626 1647 1100 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3054 3300 669 Size: 988 281 1116 246 3079 Fragments arranged by size: 3079 1116 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5710 HpaI GTT'AAC Cuts at: 3300 3300 Size: 5710 KasI G'GCGC_C Cuts at: 2020 2020 Size: 5710 KpnI G_GTAC'C Cuts at: 3035 3035 Size: 5710 MfeI C'AATT_G Cuts at: 3309 3309 Size: 5710 MluI A'CGCG_T Cuts at: 5586 5586 Size: 5710 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4824 5008 1305 Size: 3519 184 2007 Fragments arranged by size: 3519 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3888 Fragments arranged by size: 3888 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2054 3668 4027 4186 518 Size: 648 888 1614 359 159 2042 Fragments arranged by size: 2042 1614 888 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3126 3803 4951 5196 1606 Size: 1520 677 1148 245 2120 Fragments arranged by size: 2120 1520 1148 677 245 NarI GG'CG_CC Cuts at: 2021 2021 Size: 5710 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5158 NdeI CA'TA_TG Cuts at: 387 387 Size: 5710 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5710 NotI GC'GGCC_GC Cuts at: 3157 3157 Size: 5710 NspI r_CATG'y Cuts at: 1132 1229 2059 3068 4613 1132 Size: 97 830 1009 1545 2229 Fragments arranged by size: 2229 1545 1009 830 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5710 PciI A'CATG_T Cuts at: 4609 4609 Size: 5710 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5710 PmeI GTTT'AAAC Cuts at: 3014 3014 Size: 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2690 2722 3779 3811 5292 5324 2690 Size: 32 1057 32 1481 32 3076 Fragments arranged by size: 3076 1481 1057 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5710 PsiI TTA'TAA Cuts at: 3280 3280 Size: 5710 PspXI vC'TCGA_Gb Cuts at: 2307 2307 Size: 5710 PstI C_TGCA'G Cuts at: 830 3062 830 Size: 2232 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5710 SalI G'TCGA_C Cuts at: 1936 3052 1936 Size: 1116 4594 SbfI CC_TGCA'GG Cuts at: 3062 3062 Size: 5710 ScaI AGT'ACT Cuts at: 3958 5670 3958 Size: 1712 3998 SfcI C'TryA_G Cuts at: 826 1045 2529 3058 4193 4874 5065 826 Size: 219 1484 529 1135 681 191 1471 Fragments arranged by size: 1484 1471 1135 681 529 219 191 SfoI GGC'GCC Cuts at: 2022 2022 Size: 5710 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5710 SmaI CCC'GGG Cuts at: 1872 3037 1872 Size: 1165 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2307 3814 4715 4977 820 Size: 197 926 364 1507 901 262 1553 Fragments arranged by size: 1553 1507 926 901 364 262 197 SnaBI TAC'GTA Cuts at: 493 2252 3501 493 Size: 1759 1249 2702 Fragments arranged by size: 2702 1759 1249 SpeI A'CTAG_T Cuts at: 152 152 Size: 5710 SphI G_CATG'C Cuts at: 1132 3068 1132 Size: 1936 3774 SspI AAT'ATT Cuts at: 5 52 2429 3634 5 Size: 47 2377 1205 2081 Fragments arranged by size: 2377 2081 1205 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5710 StyI C'CwwG_G Cuts at: 513 1065 2904 2994 3149 513 Size: 552 1839 90 155 3074 Fragments arranged by size: 3074 1839 552 155 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2702 3737 3754 3907 4092 4537 325 Size: 1255 1122 1035 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1122 1035 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2109 2488 2889 Size: 274 80 33 51 1443 132 379 401 Cuts at: 2889 3956 5668 96 Size: 1067 1712 138 Fragments arranged by size: 1712 1443 1067 401 379 274 138 132 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2884 2916 5670 5702 2884 Size: 32 2754 32 2892 Fragments arranged by size: 2892 2754 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5710 XbaI T'CTAG_A Cuts at: 3046 3046 Size: 5710 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2572 2902 2572 Size: 330 5380 XhoI C'TCGA_G Cuts at: 1943 2307 1943 Size: 364 5346 XmaI C'CCGG_G Cuts at: 1870 3035 1870 Size: 1165 4545 XmnI GAAnn'nnTTC Cuts at: 2265 3839 2265 Size: 1574 4136 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3518 276 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI