Restriction map for
pFN21AB6734
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BseAI BsgI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DdeI DraI DraIII
DrdI DsaI Eam1105I EarI Eco47III
Eco81I EcoNI EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MseI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 343 GTCTAC GT^[AC][GT]AC
AccII 270 CGCG CG^CG
AfaI 384 GTAC GT^AC
ApaI 298 GGGCCC GGGCC^C
AvaI 295 CCCGGG C^[CT]CG[GA]G
AvaII 69 GGACC G^G[AT]CC
BanI 175 GGCACC G^G[CT][GA]CC
BbvI 233 GCTGC GCAGC(8/12)
BpmI 220 CTGGAG CTGGAG(16/14)
BsaWI 332 ACCGGA [AT]^CCGG[AT]
BsiEI 153 CGGCCG CG[GA][CT]^CG
BsoBI 295 CCCGGG C^[CT]CG[GA]G
BspMI 286 ACCTGC ACCTGC(4/8)
BsrI 18 ACTGG ACTGG(1/-1)
BstEII 282 GGTCACC G^GT[ACGT]ACC
BstNI 30 CCTGG CC^[AT]GG
BstPI 282 GGTCACC G^GT[ACGT]ACC
BstUI 270 CGCG CG^CG
CfoI 196 GCGC GCG^C
DpnII 84 GATC ^GATC
DraII 298 GGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 153 CGGCCG [CT]^GGCC[GA]
EagI 153 CGGCCG C^GGCCG
EclXI 153 CGGCCG C^GGCCG
Eco52I 153 CGGCCG C^GGCCG
EcoO109I 298 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 282 GGTCACC G^GT[ACGT]ACC
EcoRII 30 CCTGG ^CC[AT]GG
EcoT14I 167 CCAAGG C^C[AT][AT]GG
HhaI 196 GCGC GCG^C
HinP1I 196 GCGC G^CGC
HindIII 337 AAGCTT A^AGCTT
HinfI 266 GATTC G^A[ACGT]TC
MboI 84 GATC ^GATC
MboII 102 GAAGA GAAGA(8/7)
MslI 205 CACTGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 30 CCTGG CC^[AT]GG
MvnI 270 CGCG CG^CG
NdeII 84 GATC ^GATC
RsaI 384 GTAC GT^AC
Sau3AI 84 GATC ^GATC
SmaI 295 CCCGGG CCC^GGG
SmlI 352 CTCAAG C^T[CT][GA]AG
StuI 6 AGGCCT AGG^CCT
StyI 167 CCAAGG C^C[AT][AT]GG
TaqI 86 TCGA T^CGA
TfiI 266 GATTC G^A[AT]TC
TseI 233 GCTGC G^C[AT]GC
TthHB8I 86 TCGA T^CGA
XmaCI 295 CCCGGG C^CCGGG
XmaI 295 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AluI 338 AGCT AG^CT
370 AGCT
BanII 292 GAGCCC G[GA]GC[CT]^C
298 GGGCCC
BcnI 295 CCCGG CC^[GC]GG
296 CCGGG
BmyI 292 GAGCCC G[AGT]GC[ACT]^C
298 GGGCCC
BseRI 10 CTCCTC GAGGAG(10/8)
223 GAGGAG
Bsp1286I 292 GAGCCC G[AGT]GC[ACT]^C
298 GGGCCC
BstF5I 402 CATCC GGATG(2/0)
171 GGATG
BstXI 16 CCACTGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
204 CCACTGCTATGG
FokI 402 CATCC GGATG(9/13)
171 GGATG
HapII 296 CCGG C^CGG
333 CCGG
HpaII 296 CCGG C^CGG
333 CCGG
HphI 93 GGTGA GGTGA(8/7)
284 TCACC
MspI 296 CCGG C^CGG
333 CCGG
NciI 295 CCCGG CC^[GC]GG
296 CCGGG
NlaIII 199 CATG CATG^
310 CATG
TspRI 17 CACTG CA[GC]TG[ACGT][ACGT]^
205 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AciI BsaJI BsiYI BslI BssKI
Cac8I Cfr13I Fnu4HI HaeIII ItaI
MaeIII MnlI MwoI NlaIV Sau96I
ScrFI Tsp45I