Restriction map for
pFN21AB8899
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BbrPI BbsI BciVI
BclI BfaI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaI
BseAI BseRI BsiHKAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MaeI MaeIII MboII MfeI
MluI MluNI MroI MscI MseI
MslI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp45I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AlwNI 496 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 291 GGGCCC GGGCC^C
Asp700I 513 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 102 GCTGAGG CCTCAGC(-5/-2)
BcgI 554 GCATCGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 599 AGATCT A^GATCT
BsaBI 594 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 386 ACCGGA [AT]^CCGG[AT]
BsgI 307 CTGCAC GTGCAG(16/14)
BsiEI 384 CGACCG CG[GA][CT]^CG
BsiWI 122 CGTACG C^GTACG
BsmAI 137 GTCTC GTCTC(1/5)
BsmFI 299 GGGAC GGGAC(10/14)
BspMI 305 ACCTGC ACCTGC(4/8)
BsrI 501 ACTGG ACTGG(1/-1)
BstYI 599 AGATCT [GA]^GATC[CT]
DsaI 459 CCATGG C^C[GA][CT]GG
EaeI 48 CGGCCA [CT]^GGCC[GA]
EcoNI 78 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeII 151 ACGT A^CGT
MamI 594 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 599 AGATCT [GA]^GATC[CT]
NcoI 459 CCATGG C^CATGG
PflMI 495 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 248 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 70 CAGCTG CAG^CTG
TfiI 18 GAATC G^A[AT]TC
Tsp509I 652 AATT ^AATT
Van91I 495 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 182 CCAACTCGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 599 AGATCT [GA]^GATC[CT]
XmnI 513 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaII 189 GGTCC G^G[AT]CC
249 GGACC
BanII 291 GGGCCC G[GA]GC[CT]^C
399 GAGCCC
BmyI 291 GGGCCC G[AGT]GC[ACT]^C
399 GAGCCC
BpmI 502 CTGGAG CTGGAG(16/14)
508 CTGGAG
Bsp1286I 291 GGGCCC G[AGT]GC[ACT]^C
399 GAGCCC
BssSI 396 CACGAG CACGAG(-5/-1)
228 CTCGTG
BstF5I 416 GGATG GGATG(2/0)
425 GGATG
EcoT14I 412 CCTTGG C^C[AT][AT]GG
459 CCATGG
FokI 416 GGATG GGATG(9/13)
425 GGATG
HphI 160 TCACC GGTGA(8/7)
581 TCACC
SfaNI 417 GATGC GCATC(5/9)
554 GCATC
SmaI 31 CCCGGG CCC^GGG
295 CCCGGG
StyI 412 CCTTGG C^C[AT][AT]GG
459 CCATGG
TaqI 133 TCGA T^CGA
557 TCGA
TthHB8I 133 TCGA T^CGA
557 TCGA
XmaCI 31 CCCGGG C^CCGGG
295 CCCGGG
XmaI 31 CCCGGG C^CCGGG
295 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI BanI BbeI BbvI BcnI
BsaHI BsaJI BsiYI BslI BsoBI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII ItaI KasI MboI
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfoI TseI TspRI