Restriction map for pFN21AB8899
 (Linear) (Six-base) MAPSORT of: KIBB8899.seq  Check: 5425  from: 1  to: 699



KIBB8899.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     502     534     699 
   Size:        502      32     165

  Fragments arranged by size: 

                 502     165      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     501     699 
   Size:        501     198
 
ApaI G_GGCC'C

Cuts at:      0     295     699 
   Size:        295     404
 
AvaI C'yCGr_G

Cuts at:      0      31     270     295     319     699 
   Size:         31     239      25      24     380

  Fragments arranged by size: 

                 380     239      31      25      24
 
BanI G'GyrC_C

Cuts at:      0     259     379     464     545     699 
   Size:        259     120      85      81     154

  Fragments arranged by size: 

                 259     154     120      85      81
 
BanII G_rGCy'C

Cuts at:      0     295     403     699 
   Size:        295     108     296

  Fragments arranged by size: 

                 296     295     108
 
BbeI G_GCGC'C

Cuts at:      0     263     383     468     549     699 
   Size:        263     120      85      81     150

  Fragments arranged by size: 

                 263     150     120      85      81
 
BbvCI CC'TCA_GC

Cuts at:      0     103     699 
   Size:        103     596
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     543     577     699 
   Size:        543      34     122

  Fragments arranged by size: 

                 543     122      34
 
BglII A'GATC_T

Cuts at:      0     599     699 
   Size:        599     100
 
Bme1580I G_kGCm'C

Cuts at:      0     295     699 
   Size:        295     404
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523     529     699 
   Size:        523       6     170

  Fragments arranged by size: 

                 523     170       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     103     699 
   Size:        103     596
 
BsaBI GATnn'nnATC

Cuts at:      0     598     699 
   Size:        598     101
 
BsaHI Gr'CG_yC

Cuts at:      0     130     208     260     380     465     546     699 
   Size:        130      78      52     120      85      81     153

  Fragments arranged by size: 

                 153     130     120      85      81      78      52
 
BsaWI w'CCGG_w

Cuts at:      0     386     699 
   Size:        386     313
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     502     532     699 
   Size:        502      30     167

  Fragments arranged by size: 

                 502     167      30
 
BseYI C'CCAG_C

Cuts at:      0      43     664     699 
   Size:         43     621      35

  Fragments arranged by size: 

                 621      43      35
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     699 
   Size:        292     407
 
BsiEI CG_ry'CG

Cuts at:      0     387     699 
   Size:        387     312
 
BsiWI C'GTAC_G

Cuts at:      0     122     699 
   Size:        122     577
 
Bsp1286I G_dGCh'C

Cuts at:      0     295     403     699 
   Size:        295     108     296

  Fragments arranged by size: 

                 296     295     108
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     314     699 
   Size:        314     385
 
BssSI C'ACGA_G

Cuts at:      0     228     396     699 
   Size:        228     168     303

  Fragments arranged by size: 

                 303     228     168
 
BstYI r'GATC_y

Cuts at:      0     599     699 
   Size:        599     100
 
BtgI C'CryG_G

Cuts at:      0     459     699 
   Size:        459     240
 
BtsI GCAGTG_nn'

Cuts at:      0     282     699 
   Size:        282     417
 
EaeI y'GGCC_r

Cuts at:      0      48     699 
   Size:         48     651
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523     529     699 
   Size:        523       6     170

  Fragments arranged by size: 

                 523     170       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      82     699 
   Size:         82     617
 
EcoO109I rG'GnC_Cy

Cuts at:      0     107     224     249     291     292     699 
   Size:        107     117      25      42       1     407

  Fragments arranged by size: 

                 407     117     107      42      25       1
 
HaeII r_GCGC'y

Cuts at:      0     263     383     468     549     591     699 
   Size:        263     120      85      81      42     108

  Fragments arranged by size: 

                 263     120     108      85      81      42
 
KasI G'GCGC_C

Cuts at:      0     259     379     464     545     699 
   Size:        259     120      85      81     154

  Fragments arranged by size: 

                 259     154     120      85      81
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     699 
   Size:        206     493
 
MspA1I CmG'CkG

Cuts at:      0      47      72     350     423     668     699 
   Size:         47      25     278      73     245      31

  Fragments arranged by size: 

                 278     245      73      47      31      25
 
NarI GG'CG_CC

Cuts at:      0     260     380     465     546     699 
   Size:        260     120      85      81     153

  Fragments arranged by size: 

                 260     153     120      85      81
 
NcoI C'CATG_G

Cuts at:      0     459     699 
   Size:        459     240
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     501     699 
   Size:        501     198
 
PfoI T'CCnGG_A

Cuts at:      0     140     699 
   Size:        140     559
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     502     534     699 
   Size:        502      32     165

  Fragments arranged by size: 

                 502     165      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     249     699 
   Size:        249     450
 
PspOMI G'GGCC_C

Cuts at:      0     291     699 
   Size:        291     408
 
PvuII CAG'CTG

Cuts at:      0      72     699 
   Size:         72     627
 
SfoI GGC'GCC

Cuts at:      0     261     381     466     547     699 
   Size:        261     120      85      81     152

  Fragments arranged by size: 

                 261     152     120      85      81
 
SmaI CCC'GGG

Cuts at:      0      33     297     699 
   Size:         33     264     402

  Fragments arranged by size: 

                 402     264      33
 
StyI C'CwwG_G

Cuts at:      0     412     459     699 
   Size:        412      47     240

  Fragments arranged by size: 

                 412     240      47
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     177     699 
   Size:        177     522
 
TatI w'GTAC_w

Cuts at:      0     329     392     699 
   Size:        329      63     307

  Fragments arranged by size: 

                 329     307      63
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     189     699 
   Size:        189     510
 
XmaI C'CCGG_G

Cuts at:      0      31     295     699 
   Size:         31     264     404

  Fragments arranged by size: 

                 404     264      31
 
XmnI GAAnn'nnTTC

Cuts at:      0     517     699 
   Size:        517     182
 

 Enzymes that do cut:

     AloI    AlwNI     ApaI     AvaI     BanI    BanII     BbeI    BbvCI
     BcgI    BglII Bme1580I     BpmI   Bpu10I    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI    BsiWI Bsp1286I    BspMI    BssSI
    BstYI     BtgI     BtsI     EaeI  Eco57MI    EcoNI EcoO109I    HaeII
     KasI     MmeI   MspA1I     NarI     NcoI    PflMI     PfoI     PpiI
    PpuMI   PspOMI    PvuII     SfoI     SmaI     StyI    TaqII     TatI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BciVI     BclI
    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI
     BsaI    BsaAI    BseRI  BsiHKAI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MscI     MslI     NaeI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PmlI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XhoI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com