Restriction map for
pFN21AB9547
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# NO CUT ENZYMES ( 190 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AlwNI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AvaI AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI HaeIII
HapII Hin1I HincII HindII HindIII
HpaI HpaII HphI KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MluNI MroI MscI MseI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NlaIII NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI Tru9I Tsp509I
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AciI 33 CCGC CCGC(-3/-1)
AhdI 188 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 277 AGCT AG^CT
Alw44I 222 GTGCAC G^TGCAC
AlwI 154 GGATC GGATC(4/5)
ApaLI 222 GTGCAC G^TGCAC
AspEI 188 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 187 GGACC G^G[AT]CC
AviII 128 TGCGCA TGC^GCA
BanII 276 GAGCTC G[GA]GC[CT]^C
BbsI 59 GAAGAC GAAGAC(2/6)
BbvI 251 GCTGC GCAGC(8/12)
BpuAI 59 GAAGAC GAAGAC(2/6)
BsaI 195 GGTCTC GGTCTC(1/5)
BsgI 117 CTGCAC GTGCAG(16/14)
BsmAI 196 GTCTC GTCTC(1/5)
BsmFI 113 GTCCC GGGAC(10/14)
BstYI 154 GGATCT [GA]^GATC[CT]
Cfr13I 187 GGACC G^G[ACGT]CC
DdeI 281 CTAAG C^T[ACGT]AG
DraII 186 AGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 188 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 186 AGGACCT [GA]G^G[ACGT]CC[CT]
FspI 128 TGCGCA TGC^GCA
HaeII 167 GGCGCT [GA]GCGC^[CT]
HgaI 111 GCGTC GACGC(5/10)
HinfI 100 GACTC G^A[ACGT]TC
MflI 154 GGATCT [GA]^GATC[CT]
MslI 120 CACTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 43 GCTGGTGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 132 CATATG CA^TATG
PleI 100 GACTC GAGTC(4/5)
PpuMI 186 AGGACCT [GA]G^G[AT]CC[CT]
SacI 276 GAGCTC GAGCT^C
Sau96I 187 GGACC G^G[ACGT]CC
SfaNI 202 GATGC GCATC(5/9)
TseI 251 GCTGC G^C[AT]GC
Tsp45I 98 GTGAC ^GT[GC]AC
XhoII 154 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AspHI 222 GTGCAC G[AT]GC[AT]^C
276 GAGCTC
BmyI 222 GTGCAC G[AGT]GC[ACT]^C
276 GAGCTC
BsaJI 162 CCAGGG C^C[ACGT][ACGT]GG
190 CCTGGG
BsiHKAI 222 GTGCAC G[AT]GC[AT]^C
276 GAGCTC
BsiYI 33 CCGCAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
231 CCAGGAGAAGG
BslI 33 CCGCAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
231 CCAGGAGAAGG
Bsp1286I 222 GTGCAC G[AGT]GC[ACT]^C
276 GAGCTC
BstF5I 13 CATCC GGATG(2/0)
228 CATCC
Cac8I 35 GCAAGC GC[ACGT]^[ACGT]GC
39 GCAGGC
CfoI 129 GCGC GCG^C
168 GCGC
EarI 292 CTCTTC CTCTTC(1/4)
324 GAAGAG
Fnu4HI 32 GCCGC GC^[ACGT]GC
251 GCTGC
FokI 13 CATCC GGATG(9/13)
228 CATCC
HhaI 129 GCGC GCG^C
168 GCGC
HinP1I 129 GCGC G^CGC
168 GCGC
ItaI 32 GCCGC GC^[ACGT]GC
251 GCTGC
Ksp632I 292 CTCTTC CTCTTC(1/4)
324 GAAGAG
MaeIII 98 GTGAC ^GT[ACGT]AC
146 GTAAC
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
BssKI BstNI DpnII EcoRII MboI
MboII MnlI MvaI NdeII Sau3AI
ScrFI