Restriction map for pFN21AB9547
 (Circular) (Six-base) MAPSORT of: pFN21AB9547.seq  Check: 2911  from: 1  to: 5035



pFN21AB9547  5035 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:45  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2845     278 
   Size:         53      83     186     936    1309    2468

  Fragments arranged by size: 

                2468    1309     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2378    1937 
   Size:        441    4594
 
Acc65I G'GTAC_C

Cuts at:   2356    2356 
   Size:       5035
 
AclI AA'CG_TT

Cuts at:   1563    3162    3535    1563 
   Size:       1599     373    3063

  Fragments arranged by size: 

                3063    1599     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3098    4482    4750    1632 
   Size:       1466    1384     268    1917

  Fragments arranged by size: 

                1917    1466    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4838
 
AflIII A'CryG_T

Cuts at:   1203    3934    4911    1203 
   Size:       2731     977    1327

  Fragments arranged by size: 

                2731    1327     977
 
AgeI A'CCGG_T

Cuts at:   3866    3866 
   Size:       5035
 
AhdI GACnn_n'nnGTC

Cuts at:   2198    3764    2198 
   Size:       1566    3469
 
AleI CACnn'nnGTG

Cuts at:   1166    2129    1166 
   Size:        963    4072
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2147    1184 
   Size:        963    4072
 
AlwNI CAG_nnn'CTG

Cuts at:   2778    4350    2778 
   Size:       1572    3463
 
ApaLI G'TGCA_C

Cuts at:   2227    3092    4248    2227 
   Size:        865    1156    3014

  Fragments arranged by size: 

                3014    1156     865
 
ApoI r'AATT_y

Cuts at:   1493    2344    2570    2722    4831    1493 
   Size:        851     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     851     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3589     160 
   Size:        872    2557    1606

  Fragments arranged by size: 

                2557    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5035
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2360    1870 
   Size:         73     417    4545

  Fragments arranged by size: 

                4545     417      73
 
BamHI G'GATC_C

Cuts at:   2365    2732    2365 
   Size:        367    4668
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2356    3811     618 
   Size:        325     217     627     569    1455    1842

  Fragments arranged by size: 

                1842    1455     627     569     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2285    2354    1391 
   Size:        894      69    4072

  Fragments arranged by size: 

                4072     894      69
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2071     928 
   Size:        971     172    3892

  Fragments arranged by size: 

                3892     971     172
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3226    3260    1903 
   Size:         34    1289      34    3678

  Fragments arranged by size: 

                3678    1289      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2927    4137    2927 
   Size:       1210    3825
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5035
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3646     136 
   Size:        107     122      71     631     605    1974    1525

  Fragments arranged by size: 

                1974    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5030    5030 
   Size:       5035
 
BlpI GC'TnA_GC

Cuts at:   2452    2452 
   Size:       5035
 
Bme1580I G_kGCm'C

Cuts at:   1790    2231    3096    4252    1790 
   Size:        441     865    1156    2573

  Fragments arranged by size: 

                2573    1156     865     441
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5035
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2766    3724     442 
   Size:        416      53      83     399      24    1349     958    1753

  Fragments arranged by size: 

                1753    1349     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5035
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3695    1239 
   Size:       2456    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3160    4025    4323    3160 
   Size:        865     298    3872

  Fragments arranged by size: 

                3872     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2206    3698     882 
   Size:        617     707    1492    2219

  Fragments arranged by size: 

                2219    1492     707     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2826     493 
   Size:        711    1622    2702

  Fragments arranged by size: 

                2702    1622     711
 
BsaBI GATnn'nnATC

Cuts at:   5029    5029 
   Size:       5035
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2842    3224    3859     275 
   Size:         53      83     186     936    1309     382     635    1451

  Fragments arranged by size: 

                1451    1309     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3468    3866    4140    4287    4698    3468 
   Size:        398     274     147     411    3805

  Fragments arranged by size: 

                3805     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4810

  Fragments arranged by size: 

                4810     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5035
 
BseYI C'CCAG_C

Cuts at:   4238    4734    4238 
   Size:        496    4539
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2107    1163 
   Size:        573     371    4091

  Fragments arranged by size: 

                4091     573     371
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2485    3246    4274    1540 
   Size:        275     186     484     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     484     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2231    2285    3096    3181    4252    2231 
   Size:         54     811      85    1071    3014

  Fragments arranged by size: 

                3014    1071     811      85      54
 
BsmI GAATG_Cn'

Cuts at:   2546    2639    2546 
   Size:         93    4942
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2231    2285    2354    3096    3181    4252    1391 
   Size:        399     441      54      69     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     441     399      85      69      54
            
 
BspEI T'CCGG_A

Cuts at:   4698    4698 
   Size:       5035
 
BspHI T'CATG_A

Cuts at:   2924    4654    2924 
   Size:       1730    3305
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2390     844 
   Size:       1546    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2922    1394 
   Size:        429    1099    3507

  Fragments arranged by size: 

                3507    1099     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3530    3704      65 
   Size:       1183    2282     174    1396

  Fragments arranged by size: 

                2282    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3679    3866    1700 
   Size:        110    1869     187    2869

  Fragments arranged by size: 

                2869    1869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5035
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5035
 
BssSI C'ACGA_G

Cuts at:   3095    4107    3095 
   Size:       1012    4023
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5035
 
BstBI TT'CG_AA

Cuts at:   3839    3839 
   Size:       5035
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5035
 
BstYI r'GATC_y

Cuts at:   1972    2159    2365    2732    3117    3134    4586    4672    4684 
   Size:        187     206     367     385      17    1452      86      12

Cuts at:   4684    5030    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     385     367     346     206     187      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3544

  Fragments arranged by size: 

                3544     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4296

  Fragments arranged by size: 

                4296     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2551    3345    3365    2551 
   Size:        794      20    4221

  Fragments arranged by size: 

                4221     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5035
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5000
 
DraI TTT'AAA

Cuts at:   2339    2339 
   Size:       5035
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4042     809 
   Size:        507    2726    1802

  Fragments arranged by size: 

                2726    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2482    3371    5000 
   Size:         54    1425      50     275      18     652     889    1629

Cuts at:   5000       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     652     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2482    1537 
   Size:        275     670    4090

  Fragments arranged by size: 

                4090     670     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2303    2324    2965    1353 
   Size:        950      21     641    3423

  Fragments arranged by size: 

                3423     950     641      21
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3622    3996    4142     387 
   Size:       1064     138    2033     374     146    1280

  Fragments arranged by size: 

                2033    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2283    2283 
   Size:       5035
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3098    3695    4482    4750    1239 
   Size:        393    1466     597     787     268    1524

  Fragments arranged by size: 

                1524    1466     787     597     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2192    2192 
   Size:       5035
 
EcoRI G'AATT_C

Cuts at:   2344    2722    2344 
   Size:        378    4657
 
FspI TGC'GCA

Cuts at:   2135    3541    2135 
   Size:       1406    3629
 
FspAI rTGC'GCAy

Cuts at:   2135    2135 
   Size:       5035
 
HaeII r_GCGC'y

Cuts at:   1678    2176    4182    1678 
   Size:        498    2006    2531

  Fragments arranged by size: 

                2531    2006     498
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2184    2216    2257    2289    3676 
   Size:         32     135      32     612      32      41      32    1387

Cuts at:   3676    3708    3750    3782    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1387     612     135      42      41      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2379    2625     669 
   Size:        988     281     441     246    3079

  Fragments arranged by size: 

                3079     988     441     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5035
 
HpaI GTT'AAC

Cuts at:   2625    2625 
   Size:       5035
 
KpnI G_GTAC'C

Cuts at:   2360    2360 
   Size:       5035
 
MfeI C'AATT_G

Cuts at:   2634    2634 
   Size:       5035
 
MluI A'CGCG_T

Cuts at:   4911    4911 
   Size:       5035
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4149    4333    1305 
   Size:       2844     184    2007

  Fragments arranged by size: 

                2844    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3213

  Fragments arranged by size: 

                3213    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2129    2993    3352    3511     518 
   Size:        648     963     864     359     159    2042

  Fragments arranged by size: 

                2042     963     864     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2451    3128    4276    4521    1606 
   Size:        845     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     845     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3544

  Fragments arranged by size: 

                3544     939     552
 
NdeI CA'TA_TG

Cuts at:    387    2138     387 
   Size:       1751    3284
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5035
 
NotI GC'GGCC_GC

Cuts at:   2482    2482 
   Size:       5035
 
NspI r_CATG'y

Cuts at:   1132    1229    2393    3938    1132 
   Size:         97    1164    1545    2229

  Fragments arranged by size: 

                2229    1545    1164      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5035
 
PciI A'CATG_T

Cuts at:   3934    3934 
   Size:       5035
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5035
 
PfoI T'CCnGG_A

Cuts at:   2235    2235 
   Size:       5035
 
PmeI GTTT'AAAC

Cuts at:   2339    2339 
   Size:       5035
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3104    3136    4617    4649    3104 
   Size:         32    1481      32    3490

  Fragments arranged by size: 

                3490    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2192    2192 
   Size:       5035
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5035
 
PsiI TTA'TAA

Cuts at:   2605    2605 
   Size:       5035
 
PstI C_TGCA'G

Cuts at:    830    2387     830 
   Size:       1557    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5035
 
SacI G_AGCT'C

Cuts at:   2285    2285 
   Size:       5035
 
SalI G'TCGA_C

Cuts at:   1936    2377    1936 
   Size:        441    4594
 
SbfI CC_TGCA'GG

Cuts at:   2387    2387 
   Size:       5035
 
ScaI AGT'ACT

Cuts at:   3283    4995    3283 
   Size:       1712    3323
 
SfcI C'TryA_G

Cuts at:    826    1045    2383    3518    4199    4390     826 
   Size:        219    1338    1135     681     191    1471

  Fragments arranged by size: 

                1471    1338    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5035
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5035
 
SmaI CCC'GGG

Cuts at:   1872    2362    1872 
   Size:        490    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3139    4040    4302     820 
   Size:        197     926    1196     901     262    1553

  Fragments arranged by size: 

                1553    1196     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2826     493 
   Size:       2333    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5035
 
SphI G_CATG'C

Cuts at:   1132    2393    1132 
   Size:       1261    3774
 
SspI AAT'ATT

Cuts at:      5      52    2959       5 
   Size:         47    2907    2081

  Fragments arranged by size: 

                2907    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5035
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2474     513 
   Size:        552     939     470    3074

  Fragments arranged by size: 

                3074     939     552     470
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3062    3079    3232    3417    3862     325 
   Size:       1255    1482      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1482    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3281    4993      96 
   Size:        274      80      33      51    1443    1304    1712     138

  Fragments arranged by size: 

                1712    1443    1304     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4995    5027    4995 
   Size:         32    5003
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5035
 
XbaI T'CTAG_A

Cuts at:   2371    2371 
   Size:       5035
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5035
 
XmaI C'CCGG_G

Cuts at:   1870    2360    1870 
   Size:        490    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3164    3164 
   Size:       5035
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2843     276 
   Size:         53      83     186     936    1309    2468

  Fragments arranged by size: 

                2468    1309     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI
     SacI     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I   DraIII    EcoNI    EcoRV     FalI     FseI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PmlI   PspOMI    PspXI
     PsrI    PvuII    RsrII    SacII    SanDI     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI     XcmI
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