Restriction map for pFN21AE0555
 (Circular) (Six-base) MAPSORT of: pFN21AE0555.seq  Check: 7108  from: 1  to: 5836



pFN21AE0555  5836 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:43  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3646     278 
   Size:         53      83     186     936    2110    2468

  Fragments arranged by size: 

                2468    2110     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2808    3179    1937 
   Size:        871     371    4594

  Fragments arranged by size: 

                4594     871     371
 
Acc65I G'GTAC_C

Cuts at:   3157    3157 
   Size:       5836
 
AclI AA'CG_TT

Cuts at:   1563    3963    4336    1563 
   Size:       2400     373    3063

  Fragments arranged by size: 

                3063    2400     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2450    2520    2864    3899    5283    5551    1632 
   Size:        818      70     344    1035    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1035     818     344     268      70
 
AfeI AGC'GCT

Cuts at:   2096    2120    2096 
   Size:         24    5812
 
AflII C'TTAA_G

Cuts at:    820    1017    2419     820 
   Size:        197    1402    4237

  Fragments arranged by size: 

                4237    1402     197
 
AflIII A'CryG_T

Cuts at:   1203    4735    5712    1203 
   Size:       3532     977    1327

  Fragments arranged by size: 

                3532    1327     977
 
AgeI A'CCGG_T

Cuts at:   4667    4667 
   Size:       5836
 
AhdI GACnn_n'nnGTC

Cuts at:   4565    4565 
   Size:       5836
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5836
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5836
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2332    2364    2332 
   Size:         32    5804
 
AlwNI CAG_nnn'CTG

Cuts at:   2498    3579    5151    2498 
   Size:       1081    1572    3183

  Fragments arranged by size: 

                3183    1572    1081
 
ApaLI G'TGCA_C

Cuts at:   3893    5049    3893 
   Size:       1156    4680
 
ApoI r'AATT_y

Cuts at:   1493    2856    3012    3145    3371    3523    5632    1493 
   Size:       1363     156     133     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1363     226     156     152     133
 
AseI AT'TA_AT

Cuts at:    160    1032    4390     160 
   Size:        872    3358    1606

  Fragments arranged by size: 

                3358    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5836
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2132    3161    1870 
   Size:         73     189    1029    4545

  Fragments arranged by size: 

                4545    1029     189      73
 
BamHI G'GATC_C

Cuts at:   3166    3533    3166 
   Size:        367    5469
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3157    4612     618 
   Size:        325     217     627    1370    1455    1842

  Fragments arranged by size: 

                1842    1455    1370     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2464    3155    1391 
   Size:       1073     691    4072

  Fragments arranged by size: 

                4072    1073     691
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2265    2797     928 
   Size:        971     366     532    3967

  Fragments arranged by size: 

                3967     971     532     366
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4027    4061    1903 
   Size:         34    2090      34    3678

  Fragments arranged by size: 

                3678    2090      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3728    4938    3728 
   Size:       1210    4626
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5836
 
BfrBI ATG'CAT

Cuts at:   2969    2969 
   Size:       5836
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    4447     136 
   Size:        107     122      71     631     605     337    2438    1525

  Fragments arranged by size: 

                2438    1525     631     605     337     122     107      71
            
 
BglII A'GATC_T

Cuts at:   3070    5831    3070 
   Size:       2761    3075
 
BlpI GC'TnA_GC

Cuts at:   2446    3253    2446 
   Size:        807    5029
 
Bme1580I G_kGCm'C

Cuts at:   1790    3897    5053    1790 
   Size:       2107    1156    2573

  Fragments arranged by size: 

                2573    2107    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5836
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3567    4525     442 
   Size:        416      53      83     399      24    2150     958    1753

  Fragments arranged by size: 

                2150    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    2033    1056 
   Size:        977    4859
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2087    4496    1239 
   Size:        848    2409    2579

  Fragments arranged by size: 

                2579    2409     848
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2476    2892    3961    4826    5124    2476 
   Size:        416    1069     865     298    3188

  Fragments arranged by size: 

                3188    1069     865     416     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4499     882 
   Size:        617    3000    2219

  Fragments arranged by size: 

                3000    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2080    3627     493 
   Size:        711     876    1547    2702

  Fragments arranged by size: 

                2702    1547     876     711
 
BsaBI GATnn'nnATC

Cuts at:   3075    5830    3075 
   Size:       2755    3081
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3643    4025    4660     275 
   Size:         53      83     186     936    2110     382     635    1451

  Fragments arranged by size: 

                2110    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4269    4667    4941    5088    5499    4269 
   Size:        398     274     147     411    4606

  Fragments arranged by size: 

                4606     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2473    2503    1178 
   Size:         30     165      30    1070      30    4511

  Fragments arranged by size: 

                4511    1070     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5836
 
BseYI C'CCAG_C

Cuts at:   5039    5535    5039 
   Size:        496    5340
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2511    1163 
   Size:        573     775    4488

  Fragments arranged by size: 

                4488     775     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3286    4047    5075    1540 
   Size:        275     186    1285     761    1028    2301

  Fragments arranged by size: 

                2301    1285    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3897    3982    5053    3897 
   Size:         85    1071    4680

  Fragments arranged by size: 

                4680    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3347    3440    3347 
   Size:         93    5743
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2406    2406 
   Size:       5836
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2464    3155    3897    3982    5053    1391 
   Size:        399     674     691     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     691     674     399      85
 
BspEI T'CCGG_A

Cuts at:   5499    5499 
   Size:       5836
 
BspHI T'CATG_A

Cuts at:   3725    5455    3725 
   Size:       1730    4106
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2133    2233    3191     844 
   Size:       1289     100     958    3489

  Fragments arranged by size: 

                3489    1289     958     100
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2040    2053    3723    1394 
   Size:        429     217      13    1670    3507

  Fragments arranged by size: 

                3507    1670     429     217      13
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2367    2379    2682    4331    4505      65 
   Size:       1183    1119      12     303    1649     174    1396

  Fragments arranged by size: 

                1649    1396    1183    1119     303     174      12
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4480    4667    1700 
   Size:        110    2670     187    2869

  Fragments arranged by size: 

                2869    2670     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    3121      96 
   Size:       3025    2811
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5836
 
BssSI C'ACGA_G

Cuts at:   3896    4908    3896 
   Size:       1012    4824
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2162     794 
   Size:       1368    4468
 
BstBI TT'CG_AA

Cuts at:   4640    4640 
   Size:       5836
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5836
 
BstYI r'GATC_y

Cuts at:   1972    2979    3070    3166    3533    3918    3935    5387    5473 
   Size:       1007      91      96     367     385      17    1452      86

Cuts at:   5473    5485    5831    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452    1007     385     367     346      96      91
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4345

  Fragments arranged by size: 

                4345     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5097

  Fragments arranged by size: 

                5097     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2512    3352    4146    4166    2512 
   Size:        840     794      20    4182

  Fragments arranged by size: 

                4182     840     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5836
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5801
 
DraI TTT'AAA

Cuts at:   3140    3140 
   Size:       5836
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4843     809 
   Size:        507    3527    1802

  Fragments arranged by size: 

                3527    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2010    2362    2939 
   Size:         54    1425      50     275      18     180     352     577

Cuts at:   2939    3283    4172    5801       8 
   Size:        344     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     577     352     344     275     180
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3283    1537 
   Size:        275    1471    4090

  Fragments arranged by size: 

                4090    1471     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2302    3766    1353 
   Size:        949    1464    3423

  Fragments arranged by size: 

                3423    1464     949
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4423    4797    4943     387 
   Size:       1064     138    2834     374     146    1280

  Fragments arranged by size: 

                2834    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2087    2450    2520    2864    3899    4496    5283 
   Size:        393     455     363      70     344    1035     597     787

Cuts at:   5283    5551    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1035     787     597     455     393     363     344
                 268      70
 
EcoO109I rG'GnC_Cy

Cuts at:   2245    2245 
   Size:       5836
 
EcoRI G'AATT_C

Cuts at:   3145    3523    3145 
   Size:        378    5458
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2933    2933 
   Size:       5836
 
FspI TGC'GCA

Cuts at:   4342    4342 
   Size:       5836
 
HaeII r_GCGC'y

Cuts at:   1678    2098    2122    4983    1678 
   Size:        420      24    2861    2531

  Fragments arranged by size: 

                2861    2531     420      24
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4477    4509    4551    4583    1373 
   Size:         32     135      32    2905      32      42      32    2626

  Fragments arranged by size: 

                2905    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2148    3180    3426     669 
   Size:        988     281     210    1032     246    3079

  Fragments arranged by size: 

                3079    1032     988     281     246     210
 
HindIII A'AGCT_T

Cuts at:    748    2439    2846     748 
   Size:       1691     407    3738

  Fragments arranged by size: 

                3738    1691     407
 
HpaI GTT'AAC

Cuts at:   3426    3426 
   Size:       5836
 
KpnI G_GTAC'C

Cuts at:   3161    3161 
   Size:       5836
 
MfeI C'AATT_G

Cuts at:   2151    3435    2151 
   Size:       1284    4552
 
MluI A'CGCG_T

Cuts at:   5712    5712 
   Size:       5836
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2166    4950    5134    1305 
   Size:        861    2784     184    2007

  Fragments arranged by size: 

                2784    2007     861     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2364    2941      10 
   Size:         54    1425     343     532     577    2905

  Fragments arranged by size: 

                2905    1425     577     532     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2352    2372    2579    2955    3794    4153    4312 
   Size:        648    1186      20     207     376     839     359     159

Cuts at:   4312     518 
   Size:       2042

  Fragments arranged by size: 

                2042    1186     839     648     376     359     207     159
                  20
 
MspA1I CmG'CkG

Cuts at:   1606    2470    3252    3929    5077    5322    1606 
   Size:        864     782     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     864     782     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4345

  Fragments arranged by size: 

                4345     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5836
 
NheI G'CTAG_C

Cuts at:   1052    2029    1052 
   Size:        977    4859
 
NotI GC'GGCC_GC

Cuts at:   3283    3283 
   Size:       5836
 
NsiI A_TGCA'T

Cuts at:   2971    2971 
   Size:       5836
 
NspI r_CATG'y

Cuts at:   1132    1229    2521    2969    3194    4739    1132 
   Size:         97    1292     448     225    1545    2229

  Fragments arranged by size: 

                2229    1545    1292     448     225      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5836
 
PciI A'CATG_T

Cuts at:   4735    4735 
   Size:       5836
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5836
 
PfoI T'CCnGG_A

Cuts at:   2335    2335 
   Size:       5836
 
PmeI GTTT'AAAC

Cuts at:   3140    3140 
   Size:       5836
 
PmlI CAC'GTG

Cuts at:   2080    2080 
   Size:       5836
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3905    3937    5418    5450    3905 
   Size:         32    1481      32    4291

  Fragments arranged by size: 

                4291    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2245    2245 
   Size:       5836
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5836
 
PsiI TTA'TAA

Cuts at:   3406    3406 
   Size:       5836
 
PstI C_TGCA'G

Cuts at:    830    2244    3188     830 
   Size:       1414     944    3478

  Fragments arranged by size: 

                3478    1414     944
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5836
 
PvuII CAG'CTG

Cuts at:   2470    2470 
   Size:       5836
 
SalI G'TCGA_C

Cuts at:   1936    3178    1936 
   Size:       1242    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2302    2302 
   Size:       5836
 
SbfI CC_TGCA'GG

Cuts at:   2244    3188    2244 
   Size:        944    4892
 
ScaI AGT'ACT

Cuts at:   4084    5796    4084 
   Size:       1712    4124
 
SfcI C'TryA_G

Cuts at:    826    1045    2240    2805    3184    4319    5000    5191     826 
   Size:        219    1195     565     379    1135     681     191    1471

  Fragments arranged by size: 

                1471    1195    1135     681     565     379     219     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5836
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5836
 
SmaI CCC'GGG

Cuts at:   1872    3163    1872 
   Size:       1291    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2132    2419    2455    2907    3940    4841 
   Size:        197     926     189     287      36     452    1033     901

Cuts at:   4841    5103     820 
   Size:        262    1553

  Fragments arranged by size: 

                1553    1033     926     901     452     287     262     197
                 189      36
 
SnaBI TAC'GTA

Cuts at:    493    3627     493 
   Size:       3134    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5836
 
SphI G_CATG'C

Cuts at:   1132    2969    3194    1132 
   Size:       1837     225    3774

  Fragments arranged by size: 

                3774    1837     225
 
SspI AAT'ATT

Cuts at:      5      52    3760       5 
   Size:         47    3708    2081

  Fragments arranged by size: 

                3708    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5836
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3093    3275     513 
   Size:        552     939    1089     182    3074

  Fragments arranged by size: 

                3074    1089     939     552     182
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2818    3863    3880    4033    4218    4663     325 
   Size:       1255    1238    1045      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1238    1045     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2529    3121    4082 
   Size:        274      80      33      51    1443     552     592     961

Cuts at:   4082    5794      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443     961     592     552     274     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2473    2505    5796    5828    2473 
   Size:         32    3291      32    2481

  Fragments arranged by size: 

                3291    2481      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5836
 
XbaI T'CTAG_A

Cuts at:   3067    3172    3067 
   Size:        105    5731
 
XhoI C'TCGA_G

Cuts at:   1943    2132    1943 
   Size:        189    5647
 
XmaI C'CCGG_G

Cuts at:   1870    3161    1870 
   Size:       1291    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3063    3965    3063 
   Size:        902    4934
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3644     276 
   Size:         53      83     186     936    2110    2468

  Fragments arranged by size: 

                2468    2110     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NsiI
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI     PstI     PvuI    PvuII     SalI     SapI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI     BplI
   Bpu10I    BsiWI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoNI
    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     PacI   PspOMI    PspXI     PsrI    RsrII     SacI    SacII    SanDI
    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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