(Circular) (Six-base) MAPSORT of: pFN21AB6335.seq Check: 1402 from: 1 to: 5488 pFN21AB6335 5488 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3298 278 Size: 53 83 186 936 1762 2468 Fragments arranged by size: 2468 1762 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2831 1937 Size: 894 4594 Acc65I G'GTAC_C Cuts at: 2634 2809 2634 Size: 175 5313 AclI AA'CG_TT Cuts at: 1563 3615 3988 1563 Size: 2052 373 3063 Fragments arranged by size: 3063 2052 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3551 4935 5203 1632 Size: 1919 1384 268 1917 Fragments arranged by size: 1919 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5291 AflIII A'CryG_T Cuts at: 1203 4387 5364 1203 Size: 3184 977 1327 Fragments arranged by size: 3184 1327 977 AgeI A'CCGG_T Cuts at: 4319 4319 Size: 5488 AhdI GACnn_n'nnGTC Cuts at: 4217 4217 Size: 5488 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5488 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2461 1184 Size: 1277 4211 AlwNI CAG_nnn'CTG Cuts at: 3231 4803 3231 Size: 1572 3916 ApaLI G'TGCA_C Cuts at: 3545 4701 3545 Size: 1156 4332 ApoI r'AATT_y Cuts at: 1493 2385 2432 2507 2797 3023 3175 5284 1493 Size: 892 47 75 290 226 152 2109 1697 Fragments arranged by size: 2109 1697 892 290 226 152 75 47 AscI GG'CGCG_CC Cuts at: 2015 2015 Size: 5488 AseI AT'TA_AT Cuts at: 160 1032 4042 160 Size: 872 3010 1606 Fragments arranged by size: 3010 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5488 AvaI C'yCGr_G Cuts at: 1870 1943 2813 1870 Size: 73 870 4545 Fragments arranged by size: 4545 870 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2618 2651 2618 Size: 33 5455 BamHI G'GATC_C Cuts at: 2818 3185 2818 Size: 367 5121 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2634 2809 4264 618 Size: 325 217 627 847 175 1455 1842 Fragments arranged by size: 1842 1455 847 627 325 217 175 BanII G_rGCy'C Cuts at: 1391 2033 2807 1391 Size: 642 774 4072 Fragments arranged by size: 4072 774 642 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4517 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3679 3713 1903 Size: 34 1742 34 3678 Fragments arranged by size: 3678 1742 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2287 3380 4590 2287 Size: 1093 1210 3185 Fragments arranged by size: 3185 1210 1093 BclI T'GATC_A Cuts at: 1548 1548 Size: 5488 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4099 136 Size: 107 122 71 631 605 2427 1525 Fragments arranged by size: 2427 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2452 5483 2452 Size: 3031 2457 BlpI GC'TnA_GC Cuts at: 2905 2905 Size: 5488 Bme1580I G_kGCm'C Cuts at: 1790 3549 4705 1790 Size: 1759 1156 2573 Fragments arranged by size: 2573 1759 1156 BmgBI CAC'GTC Cuts at: 1318 2137 1318 Size: 819 4669 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3219 4177 442 Size: 416 53 83 399 24 1802 958 1753 Fragments arranged by size: 1802 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5488 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2083 4148 1239 Size: 844 2065 2579 Fragments arranged by size: 2579 2065 844 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2024 2045 3613 4478 4776 2024 Size: 21 1568 865 298 2736 Fragments arranged by size: 2736 1568 865 298 21 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4151 882 Size: 617 2652 2219 Fragments arranged by size: 2652 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2441 3279 493 Size: 711 1237 838 2702 Fragments arranged by size: 2702 1237 838 711 BsaBI GATnn'nnATC Cuts at: 5482 5482 Size: 5488 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3295 3677 4312 275 Size: 53 83 186 936 1762 382 635 1451 Fragments arranged by size: 1762 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3921 4319 4593 4740 5151 3921 Size: 398 274 147 411 4258 Fragments arranged by size: 4258 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2460 2490 1178 Size: 30 165 30 1057 30 4176 Fragments arranged by size: 4176 1057 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2591 1186 Size: 1405 4083 BseYI C'CCAG_C Cuts at: 2085 4691 5187 2085 Size: 2606 496 2386 Fragments arranged by size: 2606 2386 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2168 1163 Size: 573 432 4483 Fragments arranged by size: 4483 573 432 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2194 2938 3699 4727 1540 Size: 275 186 193 744 761 1028 2301 Fragments arranged by size: 2301 1028 761 744 275 193 186 BsiHKAI G_wGCw'C Cuts at: 3549 3634 4705 3549 Size: 85 1071 4332 Fragments arranged by size: 4332 1071 85 BsmI GAATG_Cn' Cuts at: 2999 3092 2999 Size: 93 5395 BsmBI CGTCTCn'nnnn_ Cuts at: 2143 2143 Size: 5488 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2807 3549 3634 4705 1391 Size: 399 243 774 742 85 1071 2174 Fragments arranged by size: 2174 1071 774 742 399 243 85 BspEI T'CCGG_A Cuts at: 5151 5151 Size: 5488 BspHI T'CATG_A Cuts at: 3377 5107 3377 Size: 1730 3758 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2121 2843 844 Size: 1277 722 3489 Fragments arranged by size: 3489 1277 722 BsrBI CCG'CTC Cuts at: 1394 1823 3375 1394 Size: 429 1552 3507 Fragments arranged by size: 3507 1552 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2260 3983 4157 65 Size: 1183 1012 1723 174 1396 Fragments arranged by size: 1723 1396 1183 1012 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2249 4132 4319 1700 Size: 110 439 1883 187 2869 Fragments arranged by size: 2869 1883 439 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5488 BssHII G'CGCG_C Cuts at: 1925 2015 1925 Size: 90 5398 BssSI C'ACGA_G Cuts at: 3548 4560 3548 Size: 1012 4476 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5488 BstBI TT'CG_AA Cuts at: 4292 4292 Size: 5488 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5488 BstYI r'GATC_y Cuts at: 1972 2452 2783 2818 3185 3570 3587 5039 5125 Size: 480 331 35 367 385 17 1452 86 Cuts at: 5125 5137 5483 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 480 385 367 346 331 86 35 17 12 BtgI C'CryG_G Cuts at: 513 1065 2205 513 Size: 552 1140 3796 Fragments arranged by size: 3796 1140 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4749 Fragments arranged by size: 4749 683 56 BtsI GCAGTG_nn' Cuts at: 3004 3798 3818 3004 Size: 794 20 4674 Fragments arranged by size: 4674 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5488 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5453 DraI TTT'AAA Cuts at: 2792 2792 Size: 5488 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4495 809 Size: 507 3179 1802 Fragments arranged by size: 3179 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2202 2935 3824 Size: 54 1425 50 275 18 372 733 889 Cuts at: 3824 5453 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 733 372 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2935 1537 Size: 275 1123 4090 Fragments arranged by size: 4090 1123 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2674 3418 1353 Size: 1321 744 3423 Fragments arranged by size: 3423 1321 744 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4075 4449 4595 387 Size: 1064 138 2486 374 146 1280 Fragments arranged by size: 2486 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2083 3551 4148 4935 5203 1239 Size: 393 451 1468 597 787 268 1524 Fragments arranged by size: 1524 1468 787 597 451 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2024 2042 2024 Size: 18 5470 EcoO109I rG'GnC_Cy Cuts at: 2065 2065 Size: 5488 EcoRI G'AATT_C Cuts at: 2385 2797 3175 2385 Size: 412 378 4698 Fragments arranged by size: 4698 412 378 EcoRV GAT'ATC Cuts at: 2263 2263 Size: 5488 FspI TGC'GCA Cuts at: 3994 3994 Size: 5488 HaeII r_GCGC'y Cuts at: 1678 2306 4635 1678 Size: 628 2329 2531 Fragments arranged by size: 2531 2329 628 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2113 4129 4161 4203 Size: 32 135 32 509 32 2016 32 42 Cuts at: 4203 4235 1373 Size: 32 2626 Fragments arranged by size: 2626 2016 509 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2832 3078 669 Size: 988 281 894 246 3079 Fragments arranged by size: 3079 988 894 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5488 HpaI GTT'AAC Cuts at: 3078 3078 Size: 5488 KpnI G_GTAC'C Cuts at: 2638 2813 2638 Size: 175 5313 MfeI C'AATT_G Cuts at: 3087 3087 Size: 5488 MluI A'CGCG_T Cuts at: 5364 5364 Size: 5488 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2031 4602 4786 1305 Size: 726 2571 184 2007 Fragments arranged by size: 2571 2007 726 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2204 10 Size: 54 1425 343 372 3294 Fragments arranged by size: 3294 1425 372 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2210 2225 2443 2597 3446 3805 3964 Size: 648 1044 15 218 154 849 359 159 Cuts at: 3964 518 Size: 2042 Fragments arranged by size: 2042 1044 849 648 359 218 159 154 15 MspA1I CmG'CkG Cuts at: 1606 2103 2187 2406 2904 3581 4729 4974 1606 Size: 497 84 219 498 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 498 497 245 219 84 NaeI GCC'GGC Cuts at: 2251 2251 Size: 5488 NcoI C'CATG_G Cuts at: 513 1065 2205 513 Size: 552 1140 3796 Fragments arranged by size: 3796 1140 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5488 NgoMIV G'CCGG_C Cuts at: 2249 2249 Size: 5488 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5488 NotI GC'GGCC_GC Cuts at: 2935 2935 Size: 5488 NspI r_CATG'y Cuts at: 1132 1229 2846 4391 1132 Size: 97 1617 1545 2229 Fragments arranged by size: 2229 1617 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5488 PciI A'CATG_T Cuts at: 4387 4387 Size: 5488 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5488 PmeI GTTT'AAAC Cuts at: 2792 2792 Size: 5488 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3557 3589 5070 5102 3557 Size: 32 1481 32 3943 Fragments arranged by size: 3943 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2065 2065 Size: 5488 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5488 PsiI TTA'TAA Cuts at: 3058 3058 Size: 5488 PstI C_TGCA'G Cuts at: 830 2118 2698 2840 830 Size: 1288 580 142 3478 Fragments arranged by size: 3478 1288 580 142 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5488 PvuII CAG'CTG Cuts at: 2406 2406 Size: 5488 SalI G'TCGA_C Cuts at: 1936 2830 1936 Size: 894 4594 SbfI CC_TGCA'GG Cuts at: 2840 2840 Size: 5488 ScaI AGT'ACT Cuts at: 3736 5448 3736 Size: 1712 3776 SfcI C'TryA_G Cuts at: 826 1045 2114 2694 2836 3971 4652 4843 826 Size: 219 1069 580 142 1135 681 191 1471 Fragments arranged by size: 1471 1135 1069 681 580 219 191 142 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5488 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5488 SmaI CCC'GGG Cuts at: 1872 2815 1872 Size: 943 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2039 2060 3592 4493 4755 820 Size: 197 926 96 21 1532 901 262 1553 Fragments arranged by size: 1553 1532 926 901 262 197 96 21 SnaBI TAC'GTA Cuts at: 493 3279 493 Size: 2786 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5488 SphI G_CATG'C Cuts at: 1132 2846 1132 Size: 1714 3774 SspI AAT'ATT Cuts at: 5 52 3412 5 Size: 47 3360 2081 Fragments arranged by size: 3360 2081 47 StuI AGG'CCT Cuts at: 1517 2081 1517 Size: 564 4924 StyI C'CwwG_G Cuts at: 513 1065 2205 2298 2927 513 Size: 552 1140 93 629 3074 Fragments arranged by size: 3074 1140 629 552 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3515 3532 3685 3870 4315 325 Size: 1255 1935 17 153 185 445 1498 Fragments arranged by size: 1935 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2400 3734 5446 Size: 274 80 33 51 1443 423 1334 1712 Cuts at: 5446 96 Size: 138 Fragments arranged by size: 1712 1443 1334 423 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5448 5480 5448 Size: 32 5456 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5488 XbaI T'CTAG_A Cuts at: 2824 2824 Size: 5488 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5488 XmaI C'CCGG_G Cuts at: 1870 2813 1870 Size: 943 4545 XmnI GAAnn'nnTTC Cuts at: 2610 3617 2610 Size: 1007 4481 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3296 276 Size: 53 83 186 936 1762 2468 Fragments arranged by size: 2468 1762 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI