Restriction map for
pFN21AE0379
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# NO CUT ENZYMES ( 96 enzymes) #
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Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AvrII BamHI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BpmI BpuAI BsaWI
BseAI BsiWI BsmI Bsp1407I BspEI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstAPI BstZ17I CpoI DraI
DraIII DrdI Eam1105I Eco47III EcoNI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KpnI MfeI MluI MroI
MseI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SfiI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I XbaI
XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 2056 GTCTAC GT^[AC][GT]AC
AlwNI 1112 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AviII 1865 TGCGCA TGC^GCA
BalI 131 TGGCCA TGG^CCA
BbeI 1588 GGCGCC GGCGC^C
BbrPI 1969 CACGTG CAC^GTG
BfaI 583 CTAG C^TAG
BlpI 1881 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1881 GCTGAGC GC^T[ACGT]AGC
BsaAI 1969 CACGTG [CT]AC^GT[GA]
BsaBI 678 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1730 CGGCCG CG[GA][CT]^CG
BspDI 84 ATCGAT AT^CGAT
BsrBI 2211 GAGCGG CCGCTC(-3/-3)
BsrFI 481 ACCGGC [GA]^CCGG[CT]
BssHII 1214 GCGCGC G^CGCGC
BstBI 868 TTCGAA TT^CGAA
BstXI 1754 CCAAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 683 AGATCC [GA]^GATC[CT]
CelII 1881 GCTGAGC GC^T[ACGT]AGC
Cfr10I 481 ACCGGC [GA]^CCGG[CT]
ClaI 84 ATCGAT AT^CGAT
EagI 1730 CGGCCG C^GGCCG
EclXI 1730 CGGCCG C^GGCCG
Eco52I 1730 CGGCCG C^GGCCG
EcoRI 391 GAATTC G^AATTC
FspI 1865 TGCGCA TGC^GCA
HincII 856 GTCAAC GT[CT]^[GA]AC
HindII 856 GTCAAC GT[CT]^[GA]AC
KasI 1588 GGCGCC G^GCGCC
KspI 1599 CCGCGG CCGC^GG
MaeI 583 CTAG C^TAG
MamI 678 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 683 AGATCC [GA]^GATC[CT]
MluNI 131 TGGCCA TGG^CCA
MscI 131 TGGCCA TGG^CCA
NarI 1588 GGCGCC GG^CGCC
NspV 868 TTCGAA TT^CGAA
PflMI 687 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1969 CACGTG CAC^GTG
PmlI 1969 CACGTG CAC^GTG
PvuII 1807 CAGCTG CAG^CTG
SacII 1599 CCGCGG CCGC^GG
SapI 2091 GCTCTTC GCTCTTC(1/4)
SfoI 1588 GGCGCC GGC^GCC
SfuI 868 TTCGAA TT^CGAA
SmaI 1724 CCCGGG CCC^GGG
SmlI 1813 CTCAAG C^T[CT][GA]AG
StuI 969 AGGCCT AGG^CCT
Van91I 687 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1901 CCAAGCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 683 AGATCC [GA]^GATC[CT]
XmaCI 1724 CCCGGG C^CCGGG
XmaI 1724 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AatII 853 GACGTC GACGT^C
1321 GACGTC
Asp700I 82 GAATCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2364 GAATAGCTTC
AspI 706 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1330 GACAACGTC
BanII 730 GAGCTC G[GA]GC[CT]^C
1825 GAGCTC
BsmBI 786 CGTCTC CGTCTC(1/5)
1892 GAGACG
BstEII 1449 GGTCACC G^GT[ACGT]ACC
1749 GGTGACC
BstPI 1449 GGTCACC G^GT[ACGT]ACC
1749 GGTGACC
EarI 1494 CTCTTC CTCTTC(1/4)
2092 CTCTTC
EcoO65I 1449 GGTCACC G^GT[ACGT]ACC
1749 GGTGACC
EcoT14I 1279 CCAAGG C^C[AT][AT]GG
1754 CCAAGG
Ksp632I 1494 CTCTTC CTCTTC(1/4)
2092 CTCTTC
MslI 123 CACGTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1302 CATAACTGTG
NlaIII 241 CATG CATG^
1038 CATG
PflFI 706 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1330 GACAACGTC
PstI 1564 CTGCAG CTGCA^G
1687 CTGCAG
SacI 730 GAGCTC GAGCT^C
1825 GAGCTC
SexAI 1745 ACCTGGT A^CC[AT]GGT
2060 ACCTGGT
StyI 1279 CCAAGG C^C[AT][AT]GG
1754 CCAAGG
Tth111I 706 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1330 GACAACGTC
XmnI 82 GAATCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2364 GAATAGCTTC
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AocI ApoI AspHI
AvaI AvaII BanI BbvI BcnI
BmyI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I