(Circular) (Six-base) MAPSORT of: pFN21AE0379.seq Check: 7030 from: 1 to: 7087 pFN21AE0379 7087 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3498 3498 Size: 7087 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2862 3330 4897 278 Size: 53 83 186 936 1326 468 1567 2468 Fragments arranged by size: 2468 1567 1326 936 468 186 83 53 AccI GT'mk_AC Cuts at: 1937 4062 4430 1937 Size: 2125 368 4594 Fragments arranged by size: 4594 2125 368 Acc65I G'GTAC_C Cuts at: 4408 4408 Size: 7087 AclI AA'CG_TT Cuts at: 1563 5214 5587 1563 Size: 3651 373 3063 Fragments arranged by size: 3651 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2486 4319 5150 6534 6802 1632 Size: 854 1833 831 1384 268 1917 Fragments arranged by size: 1917 1833 1384 854 831 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6890 AflIII A'CryG_T Cuts at: 1203 5986 6963 1203 Size: 4783 977 1327 Fragments arranged by size: 4783 1327 977 AgeI A'CCGG_T Cuts at: 5918 5918 Size: 7087 AhdI GACnn_n'nnGTC Cuts at: 5816 5816 Size: 7087 AleI CACnn'nnGTG Cuts at: 1166 2132 1166 Size: 966 6121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3329 1184 Size: 2145 4942 AlwNI CAG_nnn'CTG Cuts at: 3122 4830 6402 3122 Size: 1708 1572 3807 Fragments arranged by size: 3807 1708 1572 ApaLI G'TGCA_C Cuts at: 5144 6300 5144 Size: 1156 5931 ApoI r'AATT_y Cuts at: 1493 2396 2898 2931 3193 4396 4622 4774 6883 Size: 903 502 33 262 1203 226 152 2109 Cuts at: 6883 1493 Size: 1697 Fragments arranged by size: 2109 1697 1203 903 502 262 226 152 33 AseI AT'TA_AT Cuts at: 160 1032 5641 160 Size: 872 4609 1606 Fragments arranged by size: 4609 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7087 AvaI C'yCGr_G Cuts at: 1870 1943 2193 2720 3729 3784 4181 4412 1870 Size: 73 250 527 1009 55 397 231 4545 Fragments arranged by size: 4545 1009 527 397 250 231 73 55 BamHI G'GATC_C Cuts at: 4417 4784 4417 Size: 367 6720 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3593 3724 4177 4220 4408 Size: 325 217 627 1806 131 453 43 188 Cuts at: 4408 5863 618 Size: 1455 1842 Fragments arranged by size: 1842 1806 1455 627 453 325 217 188 131 43 BanII G_rGCy'C Cuts at: 1391 2739 3834 4406 1391 Size: 1348 1095 572 4072 Fragments arranged by size: 4072 1348 1095 572 BbeI G_GCGC'C Cuts at: 3597 3597 Size: 7087 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6116 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5278 5312 1903 Size: 34 3341 34 3678 Fragments arranged by size: 3678 3341 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4979 6189 4979 Size: 1210 5877 BclI T'GATC_A Cuts at: 1548 1548 Size: 7087 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5698 136 Size: 107 122 71 631 605 4026 1525 Fragments arranged by size: 4026 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7082 7082 Size: 7087 BlpI GC'TnA_GC Cuts at: 3887 4504 3887 Size: 617 6470 Bme1580I G_kGCm'C Cuts at: 1790 3729 4182 4334 5148 6304 1790 Size: 1939 453 152 814 1156 2573 Fragments arranged by size: 2573 1939 1156 814 453 152 BmgBI CAC'GTC Cuts at: 1318 2947 3016 1318 Size: 1629 69 5389 Fragments arranged by size: 5389 1629 69 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4818 5776 442 Size: 416 53 83 399 24 3401 958 1753 Fragments arranged by size: 3401 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7087 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2432 2432 Size: 7087 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5747 1239 Size: 4508 2579 Bpu10I CC'TnA_GC Cuts at: 2730 2730 Size: 7087 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3803 5212 6077 6375 3803 Size: 1409 865 298 4515 Fragments arranged by size: 4515 1409 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2661 3325 3514 5750 882 Size: 617 1162 664 189 2236 2219 Fragments arranged by size: 2236 2219 1162 664 617 189 BsaAI yAC'GTr Cuts at: 493 1204 3976 4878 493 Size: 711 2772 902 2702 Fragments arranged by size: 2772 2702 902 711 BsaBI GATnn'nnATC Cuts at: 2687 7081 2687 Size: 4394 2693 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2859 3327 3594 3657 Size: 53 83 186 936 1326 468 267 63 Cuts at: 3657 4894 5276 5911 275 Size: 1237 382 635 1451 Fragments arranged by size: 1451 1326 1237 936 635 468 382 267 186 83 63 53 BsaWI w'CCGG_w Cuts at: 5520 5918 6192 6339 6750 5520 Size: 398 274 147 411 5857 Fragments arranged by size: 5857 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2270 2300 3593 3623 3980 Size: 30 165 30 867 30 1293 30 357 Cuts at: 3980 4010 1178 Size: 30 4255 Fragments arranged by size: 4255 1293 867 357 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 2294 2624 4159 4314 1186 Size: 940 168 330 1535 155 3959 Fragments arranged by size: 3959 1535 940 330 168 155 BseYI C'CCAG_C Cuts at: 3297 3999 6290 6786 3297 Size: 702 2291 496 3598 Fragments arranged by size: 3598 2291 702 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3335 3386 3697 3956 4159 1163 Size: 573 1599 51 311 259 203 4091 Fragments arranged by size: 4091 1599 573 311 259 203 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3738 4537 5298 6326 1540 Size: 275 186 1737 799 761 1028 2301 Fragments arranged by size: 2301 1737 1028 799 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2739 3834 3981 4116 5148 5233 6304 2739 Size: 1095 147 135 1032 85 1071 3522 Fragments arranged by size: 3522 1095 1071 1032 147 135 85 BsmI GAATG_Cn' Cuts at: 4598 4691 4598 Size: 93 6994 BsmBI CGTCTCn'nnnn_ Cuts at: 2797 3891 2797 Size: 1094 5993 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2739 3729 3834 3981 4116 4182 4334 Size: 399 949 990 105 147 135 66 152 Cuts at: 4334 4406 5148 5233 6304 1391 Size: 72 742 85 1071 2174 Fragments arranged by size: 2174 1071 990 949 742 399 152 147 135 105 85 72 66 BspEI T'CCGG_A Cuts at: 6750 6750 Size: 7087 BspHI T'CATG_A Cuts at: 4976 6706 4976 Size: 1730 5357 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3307 3498 4017 4442 844 Size: 2463 191 519 425 3489 Fragments arranged by size: 3489 2463 519 425 191 BsrBI CCG'CTC Cuts at: 1394 1823 4218 4974 1394 Size: 429 2395 756 3507 Fragments arranged by size: 3507 2395 756 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5582 5756 65 Size: 1183 4334 174 1396 Fragments arranged by size: 4334 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2486 5731 5918 1700 Size: 110 676 3245 187 2869 Fragments arranged by size: 3245 2869 676 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7087 BssHII G'CGCG_C Cuts at: 1925 3219 1925 Size: 1294 5793 BssSI C'ACGA_G Cuts at: 5147 6159 5147 Size: 1012 6075 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7087 BstBI TT'CG_AA Cuts at: 2874 5891 2874 Size: 3017 4070 BstEII G'GTnAC_C Cuts at: 1184 3454 3754 1184 Size: 2270 300 4517 Fragments arranged by size: 4517 2270 300 BstXI CCAn_nnnn'nTGG Cuts at: 3766 3766 Size: 7087 BstYI r'GATC_y Cuts at: 1972 2688 4417 4784 5169 5186 6638 6724 6736 Size: 716 1729 367 385 17 1452 86 12 Cuts at: 6736 7082 1972 Size: 346 1977 Fragments arranged by size: 1977 1729 1452 716 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3545 4020 4326 3545 Size: 475 306 6306 Fragments arranged by size: 6306 475 306 BtgI C'CryG_G Cuts at: 513 1065 3186 3604 3825 513 Size: 552 2121 418 221 3775 Fragments arranged by size: 3775 2121 552 418 221 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6348 Fragments arranged by size: 6348 683 56 BtsI GCAGTG_nn' Cuts at: 4603 5397 5417 4603 Size: 794 20 6273 Fragments arranged by size: 6273 794 20 ClaI AT'CG_AT Cuts at: 1554 2090 1554 Size: 536 6551 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7052 DraI TTT'AAA Cuts at: 4391 4391 Size: 7087 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6094 809 Size: 507 4778 1802 Fragments arranged by size: 4778 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2136 3529 3735 Size: 54 1425 50 275 18 306 1393 206 Cuts at: 3735 3957 4534 5423 7052 8 Size: 222 577 889 1629 43 Fragments arranged by size: 1629 1425 1393 889 577 306 275 222 206 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3735 4534 1537 Size: 275 1923 799 4090 Fragments arranged by size: 4090 1923 799 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3505 4103 5017 1353 Size: 2152 598 914 3423 Fragments arranged by size: 3423 2152 914 598 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2649 5674 6048 6194 387 Size: 1064 138 1060 3025 374 146 1280 Fragments arranged by size: 3025 1280 1064 1060 374 146 138 EcoICRI GAG'CTC Cuts at: 2737 3832 2737 Size: 1095 5992 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2486 4319 5150 5747 6534 6802 1239 Size: 393 854 1833 831 597 787 268 1524 Fragments arranged by size: 1833 1524 854 831 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2152 2673 3007 3481 3565 4187 4227 2152 Size: 521 334 474 84 622 40 5012 Fragments arranged by size: 5012 622 521 474 334 84 40 EcoRI G'AATT_C Cuts at: 2396 4396 4774 2396 Size: 2000 378 4709 Fragments arranged by size: 4709 2000 378 FspI TGC'GCA Cuts at: 3872 5593 3872 Size: 1721 5366 FspAI rTGC'GCAy Cuts at: 3872 3872 Size: 7087 HaeII r_GCGC'y Cuts at: 1678 2193 3423 3597 3635 6234 1678 Size: 515 1230 174 38 2599 2531 Fragments arranged by size: 2599 2531 1230 515 174 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2639 2671 2801 2833 3104 Size: 32 135 32 1067 32 130 32 271 Cuts at: 3104 3136 3419 3451 3593 3625 3767 3799 3980 Size: 32 283 32 142 32 142 32 181 Cuts at: 3980 4012 5728 5760 5802 5834 1373 Size: 32 1716 32 42 32 2626 Fragments arranged by size: 2626 1716 1067 283 271 181 142 142 135 130 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2863 4431 4677 669 Size: 988 281 925 1568 246 3079 Fragments arranged by size: 3079 1568 988 925 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7087 HpaI GTT'AAC Cuts at: 4677 4677 Size: 7087 KasI G'GCGC_C Cuts at: 3593 3593 Size: 7087 KpnI G_GTAC'C Cuts at: 4412 4412 Size: 7087 MfeI C'AATT_G Cuts at: 4686 4686 Size: 7087 MluI A'CGCG_T Cuts at: 6963 6963 Size: 7087 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2547 2580 3348 6201 6385 1305 Size: 1242 33 768 2853 184 2007 Fragments arranged by size: 2853 2007 1242 768 184 33 MscI TGG'CCA Cuts at: 10 64 1489 1832 2138 10 Size: 54 1425 343 306 4959 Fragments arranged by size: 4959 1425 343 306 54 MslI CAynn'nnrTG Cuts at: 518 1166 2132 3311 5045 5404 5563 518 Size: 648 966 1179 1734 359 159 2042 Fragments arranged by size: 2042 1734 1179 966 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3606 3691 3814 4503 5180 6328 6573 1606 Size: 2000 85 123 689 677 1148 245 2120 Fragments arranged by size: 2120 2000 1148 689 677 245 123 85 NarI GG'CG_CC Cuts at: 3594 3594 Size: 7087 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6535 NdeI CA'TA_TG Cuts at: 387 387 Size: 7087 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7087 NotI GC'GGCC_GC Cuts at: 4534 4534 Size: 7087 NspI r_CATG'y Cuts at: 1132 1229 4445 5990 1132 Size: 97 3216 1545 2229 Fragments arranged by size: 3216 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7087 PciI A'CATG_T Cuts at: 5986 5986 Size: 7087 PflMI CCAn_nnn'nTGG Cuts at: 1108 2698 1108 Size: 1590 5497 PfoI T'CCnGG_A Cuts at: 2574 3009 2574 Size: 435 6652 PmeI GTTT'AAAC Cuts at: 4391 4391 Size: 7087 PmlI CAC'GTG Cuts at: 3976 3976 Size: 7087 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4277 4309 5156 5188 6669 6701 4277 Size: 32 847 32 1481 32 4663 Fragments arranged by size: 4663 1481 847 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2152 2673 3007 3481 4187 4227 2152 Size: 521 334 474 706 40 5012 Fragments arranged by size: 5012 706 521 474 334 40 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7087 PsiI TTA'TAA Cuts at: 4657 4657 Size: 7087 PstI C_TGCA'G Cuts at: 830 3573 3696 4439 830 Size: 2743 123 743 3478 Fragments arranged by size: 3478 2743 743 123 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7087 PvuII CAG'CTG Cuts at: 3814 3814 Size: 7087 SacI G_AGCT'C Cuts at: 2739 3834 2739 Size: 1095 5992 SacII CC_GC'GG Cuts at: 3607 3607 Size: 7087 SalI G'TCGA_C Cuts at: 1936 4429 1936 Size: 2493 4594 SanDI GG'GwC_CC Cuts at: 4187 4227 4187 Size: 40 7047 SapI GCTCTTCn'nnn_ Cuts at: 4103 4103 Size: 7087 SbfI CC_TGCA'GG Cuts at: 4439 4439 Size: 7087 ScaI AGT'ACT Cuts at: 5335 7047 5335 Size: 1712 5375 SexAI A'CCwGG_T Cuts at: 3750 4065 3750 Size: 315 6772 SfcI C'TryA_G Cuts at: 826 1045 2961 3569 3692 4435 5570 6251 6442 Size: 219 1916 608 123 743 1135 681 191 Cuts at: 6442 826 Size: 1471 Fragments arranged by size: 1916 1471 1135 743 681 608 219 191 123 SfoI GGC'GCC Cuts at: 3595 3595 Size: 7087 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7087 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7087 SmaI CCC'GGG Cuts at: 1872 3731 4414 1872 Size: 1859 683 4545 Fragments arranged by size: 4545 1859 683 SmlI C'TyrA_G Cuts at: 820 1017 1943 3818 5191 6092 6354 820 Size: 197 926 1875 1373 901 262 1553 Fragments arranged by size: 1875 1553 1373 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4878 493 Size: 4385 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7087 SphI G_CATG'C Cuts at: 1132 4445 1132 Size: 3313 3774 SspI AAT'ATT Cuts at: 5 52 5011 5 Size: 47 4959 2081 Fragments arranged by size: 4959 2081 47 StuI AGG'CCT Cuts at: 1517 2976 1517 Size: 1459 5628 StyI C'CwwG_G Cuts at: 513 1065 3284 3759 4526 513 Size: 552 2219 475 767 3074 Fragments arranged by size: 3074 2219 767 552 475 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3368 3824 5114 5131 5284 5469 5914 Size: 1255 1788 456 1290 17 153 185 445 Cuts at: 5914 325 Size: 1498 Fragments arranged by size: 1788 1498 1290 1255 456 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2020 3246 4248 Size: 274 80 33 51 1443 43 1226 1002 Cuts at: 4248 5333 7045 96 Size: 1085 1712 138 Fragments arranged by size: 1712 1443 1226 1085 1002 274 138 80 51 43 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2151 2183 2270 2302 7047 7079 2151 Size: 32 87 32 4745 32 2159 Fragments arranged by size: 4745 2159 87 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2714 3338 1316 Size: 1398 624 5065 Fragments arranged by size: 5065 1398 624 XbaI T'CTAG_A Cuts at: 4423 4423 Size: 7087 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3913 3913 Size: 7087 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7087 XmaI C'CCGG_G Cuts at: 1870 3729 4412 1870 Size: 1859 683 4545 Fragments arranged by size: 4545 1859 683 XmnI GAAnn'nnTTC Cuts at: 2091 4373 5216 2091 Size: 2282 843 3962 Fragments arranged by size: 3962 2282 843 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2860 3328 4895 276 Size: 53 83 186 936 1326 468 1567 2468 Fragments arranged by size: 2468 1567 1326 936 468 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SfiI SrfI SwaI