(Circular) (Six-base) MAPSORT of: pFN21AE0010.seq Check: 3188 from: 1 to: 7015 pFN21AE0010 7015 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3030 3030 Size: 7015 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4825 278 Size: 53 83 186 936 3289 2468 Fragments arranged by size: 3289 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3091 4358 1937 Size: 1154 1267 4594 Fragments arranged by size: 4594 1267 1154 Acc65I G'GTAC_C Cuts at: 4336 4336 Size: 7015 AclI AA'CG_TT Cuts at: 1563 5142 5515 1563 Size: 3579 373 3063 Fragments arranged by size: 3579 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2412 3363 5078 6462 6730 1632 Size: 780 951 1715 1384 268 1917 Fragments arranged by size: 1917 1715 1384 951 780 268 AflII C'TTAA_G Cuts at: 820 1017 3709 820 Size: 197 2692 4126 Fragments arranged by size: 4126 2692 197 AflIII A'CryG_T Cuts at: 1203 5914 6891 1203 Size: 4711 977 1327 Fragments arranged by size: 4711 1327 977 AgeI A'CCGG_T Cuts at: 5846 5846 Size: 7015 AhdI GACnn_n'nnGTC Cuts at: 5744 5744 Size: 7015 AleI CACnn'nnGTG Cuts at: 1166 4037 1166 Size: 2871 4144 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7015 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2379 2411 4142 4174 2379 Size: 32 1731 32 5220 Fragments arranged by size: 5220 1731 32 32 AlwNI CAG_nnn'CTG Cuts at: 2068 2379 2447 2629 3341 4115 4270 4758 6330 Size: 311 68 182 712 774 155 488 1572 Cuts at: 6330 2068 Size: 2753 Fragments arranged by size: 2753 1572 774 712 488 311 182 155 68 ApaLI G'TGCA_C Cuts at: 5072 6228 5072 Size: 1156 5859 ApoI r'AATT_y Cuts at: 1493 2368 3687 4324 4550 4702 6811 1493 Size: 875 1319 637 226 152 2109 1697 Fragments arranged by size: 2109 1697 1319 875 637 226 152 AseI AT'TA_AT Cuts at: 160 1032 5569 160 Size: 872 4537 1606 Fragments arranged by size: 4537 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7015 AvaI C'yCGr_G Cuts at: 1870 1943 4340 1870 Size: 73 2397 4545 Fragments arranged by size: 4545 2397 73 BamHI G'GATC_C Cuts at: 3199 4345 4712 3199 Size: 1146 367 5502 Fragments arranged by size: 5502 1146 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2582 4336 5791 618 Size: 325 217 627 795 1754 1455 1842 Fragments arranged by size: 1842 1754 1455 795 627 325 217 BanII G_rGCy'C Cuts at: 1391 3446 4334 1391 Size: 2055 888 4072 Fragments arranged by size: 4072 2055 888 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4101 928 Size: 971 2202 3842 Fragments arranged by size: 3842 2202 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5206 5240 1903 Size: 34 3269 34 3678 Fragments arranged by size: 3678 3269 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3782 4302 4907 6117 3782 Size: 520 605 1210 4680 Fragments arranged by size: 4680 1210 605 520 BclI T'GATC_A Cuts at: 1548 2092 1548 Size: 544 6471 BfrBI ATG'CAT Cuts at: 2057 2057 Size: 7015 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2473 5626 136 Size: 107 122 71 631 605 801 3153 1525 Fragments arranged by size: 3153 1525 801 631 605 122 107 71 BglII A'GATC_T Cuts at: 3035 7010 3035 Size: 3975 3040 BlpI GC'TnA_GC Cuts at: 4432 4432 Size: 7015 Bme1580I G_kGCm'C Cuts at: 1790 5076 6232 1790 Size: 3286 1156 2573 Fragments arranged by size: 3286 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7015 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2078 4746 5704 Size: 416 53 83 399 24 661 2668 958 Cuts at: 5704 442 Size: 1753 Fragments arranged by size: 2668 1753 958 661 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7015 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2718 2718 Size: 7015 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2771 3344 3431 4028 4301 5675 1239 Size: 1532 573 87 597 273 1374 2579 Fragments arranged by size: 2579 1532 1374 597 573 273 87 Bpu10I CC'TnA_GC Cuts at: 2427 4082 2427 Size: 1655 5360 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5140 6005 6303 5140 Size: 865 298 5852 Fragments arranged by size: 5852 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3247 5678 882 Size: 617 1748 2431 2219 Fragments arranged by size: 2431 2219 1748 617 BsaAI yAC'GTr Cuts at: 493 1204 4806 493 Size: 711 3602 2702 Fragments arranged by size: 3602 2702 711 BsaBI GATnn'nnATC Cuts at: 7009 7009 Size: 7015 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4822 5204 5839 275 Size: 53 83 186 936 3289 382 635 1451 Fragments arranged by size: 3289 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3058 3168 5448 5846 6120 6267 6678 3058 Size: 110 2280 398 274 147 411 3395 Fragments arranged by size: 3395 2280 411 398 274 147 110 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2971 3001 3317 3347 3618 Size: 30 165 30 1568 30 316 30 271 Cuts at: 3618 3648 4142 4172 1178 Size: 30 494 30 4021 Fragments arranged by size: 4021 1568 494 316 271 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2267 2823 3574 4061 1186 Size: 1081 556 751 487 4140 Fragments arranged by size: 4140 1081 751 556 487 BseYI C'CCAG_C Cuts at: 2609 2840 2935 6218 6714 2609 Size: 231 95 3283 496 2910 Fragments arranged by size: 3283 2910 496 231 95 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2145 2348 4020 1163 Size: 573 409 203 1672 4158 Fragments arranged by size: 4158 1672 573 409 203 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4465 5226 6254 1540 Size: 275 186 2464 761 1028 2301 Fragments arranged by size: 2464 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2797 2815 2968 3417 4044 5076 5161 6232 2797 Size: 18 153 449 627 1032 85 1071 3580 Fragments arranged by size: 3580 1071 1032 627 449 153 85 18 BsmI GAATG_Cn' Cuts at: 4526 4619 4526 Size: 93 6922 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2797 2815 2968 3417 3446 4044 4334 Size: 399 1007 18 153 449 29 598 290 Cuts at: 4334 5076 5161 6232 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 1007 742 598 449 399 290 153 85 29 18 BspEI T'CCGG_A Cuts at: 6678 6678 Size: 7015 BspHI T'CATG_A Cuts at: 3843 4904 6634 3843 Size: 1061 1730 4224 Fragments arranged by size: 4224 1730 1061 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3030 3667 4370 844 Size: 2186 637 703 3489 Fragments arranged by size: 3489 2186 703 637 BsrBI CCG'CTC Cuts at: 1394 1823 4902 1394 Size: 429 3079 3507 Fragments arranged by size: 3507 3079 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2218 5510 5684 65 Size: 1183 970 3292 174 1396 Fragments arranged by size: 3292 1396 1183 970 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4260 5659 5846 1700 Size: 110 2450 1399 187 2869 Fragments arranged by size: 2869 2450 1399 187 110 BsrGI T'GTAC_A Cuts at: 96 3757 96 Size: 3661 3354 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7015 BssSI C'ACGA_G Cuts at: 4170 5075 6087 4170 Size: 905 1012 5098 Fragments arranged by size: 5098 1012 905 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7015 BstBI TT'CG_AA Cuts at: 5819 5819 Size: 7015 BstEII G'GTnAC_C Cuts at: 1184 2243 2744 1184 Size: 1059 501 5455 Fragments arranged by size: 5455 1059 501 BstYI r'GATC_y Cuts at: 1972 2305 2387 3035 3199 4345 4712 5097 5114 Size: 333 82 648 164 1146 367 385 17 Cuts at: 5114 6566 6652 6664 7010 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1146 648 385 367 346 333 164 86 82 17 12 Bsu36I CC'TnA_GG Cuts at: 3396 3396 Size: 7015 BtgI C'CryG_G Cuts at: 513 1065 4087 513 Size: 552 3022 3441 Fragments arranged by size: 3441 3022 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6276 Fragments arranged by size: 6276 683 56 BtsI GCAGTG_nn' Cuts at: 2331 2417 2725 4032 4064 4531 5325 5345 2331 Size: 86 308 1307 32 467 794 20 4001 Fragments arranged by size: 4001 1307 794 467 308 86 32 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7015 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6980 DraI TTT'AAA Cuts at: 4319 4319 Size: 7015 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6022 809 Size: 507 4706 1802 Fragments arranged by size: 4706 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3402 4203 4462 Size: 54 1425 50 275 18 1572 801 259 Cuts at: 4462 5351 6980 8 Size: 889 1629 43 Fragments arranged by size: 1629 1572 1425 889 801 275 259 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4462 1537 Size: 275 2650 4090 Fragments arranged by size: 4090 2650 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2079 2737 3645 3779 4945 1353 Size: 726 658 908 134 1166 3423 Fragments arranged by size: 3423 1166 908 726 658 134 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5602 5976 6122 387 Size: 1064 138 4013 374 146 1280 Fragments arranged by size: 4013 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2412 2771 3344 3363 3431 4028 4301 Size: 393 780 359 573 19 68 597 273 Cuts at: 4301 5078 5675 6462 6730 1239 Size: 777 597 787 268 1524 Fragments arranged by size: 1524 787 780 777 597 597 573 393 359 273 268 68 19 EcoNI CCTnn'n_nnAGG Cuts at: 3009 3009 Size: 7015 EcoO109I rG'GnC_Cy Cuts at: 2475 2503 2857 3014 3043 2475 Size: 28 354 157 29 6447 Fragments arranged by size: 6447 354 157 29 28 EcoRI G'AATT_C Cuts at: 2368 4324 4702 2368 Size: 1956 378 4681 Fragments arranged by size: 4681 1956 378 FspI TGC'GCA Cuts at: 5521 5521 Size: 7015 HaeII r_GCGC'y Cuts at: 1678 2161 6162 1678 Size: 483 4001 2531 Fragments arranged by size: 4001 2531 483 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2617 2649 2684 2716 3163 Size: 32 135 32 1045 32 35 32 447 Cuts at: 3163 3195 3227 3259 3317 3349 3618 3650 5656 Size: 32 32 32 58 32 269 32 2006 Cuts at: 5656 5688 5730 5762 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2006 1045 447 269 135 58 42 35 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2535 3092 4258 4359 4605 669 Size: 988 281 597 557 1166 101 246 3079 Fragments arranged by size: 3079 1166 988 597 557 281 246 101 HindIII A'AGCT_T Cuts at: 748 3383 3455 748 Size: 2635 72 4308 Fragments arranged by size: 4308 2635 72 HpaI GTT'AAC Cuts at: 4605 4605 Size: 7015 KpnI G_GTAC'C Cuts at: 4340 4340 Size: 7015 MfeI C'AATT_G Cuts at: 4614 4614 Size: 7015 MluI A'CGCG_T Cuts at: 6891 6891 Size: 7015 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6129 6313 1305 Size: 4824 184 2007 Fragments arranged by size: 4824 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3404 4205 10 Size: 54 1425 343 1572 801 2820 Fragments arranged by size: 2820 1572 1425 801 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3842 4037 4973 5332 5491 518 Size: 648 2676 195 936 359 159 2042 Fragments arranged by size: 2676 2042 936 648 359 195 159 MspA1I CmG'CkG Cuts at: 1606 3226 3338 3833 4431 5108 6256 6501 1606 Size: 1620 112 495 598 677 1148 245 2120 Fragments arranged by size: 2120 1620 1148 677 598 495 245 112 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6463 NdeI CA'TA_TG Cuts at: 387 3205 387 Size: 2818 4197 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7015 NotI GC'GGCC_GC Cuts at: 4462 4462 Size: 7015 NsiI A_TGCA'T Cuts at: 2059 2059 Size: 7015 NspI r_CATG'y Cuts at: 1132 1229 3700 4373 5918 1132 Size: 97 2471 673 1545 2229 Fragments arranged by size: 2471 2229 1545 673 97 PasI CC'CwG_GG Cuts at: 1348 2482 1348 Size: 1134 5881 PciI A'CATG_T Cuts at: 5914 5914 Size: 7015 PflMI CCAn_nnn'nTGG Cuts at: 1108 2379 1108 Size: 1271 5744 PmeI GTTT'AAAC Cuts at: 4319 4319 Size: 7015 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4142 4174 5084 5116 6597 6629 4142 Size: 32 910 32 1481 32 4528 Fragments arranged by size: 4528 1481 910 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2503 2857 3014 2503 Size: 354 157 6504 Fragments arranged by size: 6504 354 157 PshAI GACnn'nnGTC Cuts at: 1613 2833 1613 Size: 1220 5795 PsiI TTA'TAA Cuts at: 3578 4585 3578 Size: 1007 6008 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2505 2537 2505 Size: 32 6983 PstI C_TGCA'G Cuts at: 830 2720 4296 4367 830 Size: 1890 1576 71 3478 Fragments arranged by size: 3478 1890 1576 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7015 PvuII CAG'CTG Cuts at: 3338 3833 3338 Size: 495 6520 SalI G'TCGA_C Cuts at: 1936 3090 4357 1936 Size: 1154 1267 4594 Fragments arranged by size: 4594 1267 1154 SanDI GG'GwC_CC Cuts at: 2857 2857 Size: 7015 SapI GCTCTTCn'nnn_ Cuts at: 3645 3645 Size: 7015 SbfI CC_TGCA'GG Cuts at: 4367 4367 Size: 7015 ScaI AGT'ACT Cuts at: 5263 6975 5263 Size: 1712 5303 SfcI C'TryA_G Cuts at: 826 1045 2439 2716 2819 3592 4100 4119 4292 Size: 219 1394 277 103 773 508 19 173 Cuts at: 4292 4363 5498 6179 6370 826 Size: 71 1135 681 191 1471 Fragments arranged by size: 1471 1394 1135 773 681 508 277 219 191 173 103 71 19 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7015 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7015 SmaI CCC'GGG Cuts at: 1872 4342 1872 Size: 2470 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3709 5119 6020 6282 820 Size: 197 926 1766 1410 901 262 1553 Fragments arranged by size: 1766 1553 1410 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4806 493 Size: 4313 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7015 SphI G_CATG'C Cuts at: 1132 4373 1132 Size: 3241 3774 SspI AAT'ATT Cuts at: 5 52 4939 5 Size: 47 4887 2081 Fragments arranged by size: 4887 2081 47 StuI AGG'CCT Cuts at: 1517 3739 1517 Size: 2222 4793 StyI C'CwwG_G Cuts at: 513 1065 2525 4454 513 Size: 552 1460 1929 3074 Fragments arranged by size: 3074 1929 1460 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2231 2633 5042 5059 5212 5397 5842 Size: 1255 651 402 2409 17 153 185 445 Cuts at: 5842 325 Size: 1498 Fragments arranged by size: 2409 1498 1255 651 445 402 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3757 5261 6973 Size: 274 80 33 51 1443 1780 1504 1712 Cuts at: 6973 96 Size: 138 Fragments arranged by size: 1780 1712 1504 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2931 2963 6975 7007 2931 Size: 32 4012 32 2939 Fragments arranged by size: 4012 2939 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7015 XbaI T'CTAG_A Cuts at: 4351 4351 Size: 7015 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4052 4052 Size: 7015 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7015 XmaI C'CCGG_G Cuts at: 1870 4340 1870 Size: 2470 4545 XmnI GAAnn'nnTTC Cuts at: 5144 5144 Size: 7015 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4823 276 Size: 53 83 186 936 3289 2468 Fragments arranged by size: 3289 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI