Restriction map for
pFN21AE0010
(Linear) (Six-base) MAPSORT of: KIEE0010.seq Check: 6150 from: 1 to: 2310
KIEE0010.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1025 2310
Size: 1025 1285
AccI GT'mk_AC
Cuts at: 0 1086 2310
Size: 1086 1224
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 1358 2310
Size: 407 951 952
Fragments arranged by size:
952 951 407
AflII C'TTAA_G
Cuts at: 0 1704 2310
Size: 1704 606
AleI CACnn'nnGTG
Cuts at: 0 2032 2310
Size: 2032 278
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 374 406 2137 2169 2310
Size: 374 32 1731 32 141
Fragments arranged by size:
1731 374 141 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 63 374 442 624 1336 2110 2265 2310
Size: 63 311 68 182 712 774 155 45
Fragments arranged by size:
774 712 311 182 155 68 63 45
ApoI r'AATT_y
Cuts at: 0 363 1682 2310
Size: 363 1319 628
Fragments arranged by size:
1319 628 363
BamHI G'GATC_C
Cuts at: 0 1194 2310
Size: 1194 1116
BanI G'GyrC_C
Cuts at: 0 577 2310
Size: 577 1733
BanII G_rGCy'C
Cuts at: 0 1441 2310
Size: 1441 869
BbsI GAAGACnn'nnnn_
Cuts at: 0 2096 2310
Size: 2096 214
BciVI GTATCCnnnnn_n'
Cuts at: 0 1777 2297 2310
Size: 1777 520 13
Fragments arranged by size:
1777 520 13
BclI T'GATC_A
Cuts at: 0 87 2310
Size: 87 2223
BfrBI ATG'CAT
Cuts at: 0 52 2310
Size: 52 2258
BglI GCCn_nnn'nGGC
Cuts at: 0 468 2310
Size: 468 1842
BglII A'GATC_T
Cuts at: 0 1030 2310
Size: 1030 1280
BmrI ACTGGGnnnn_n'
Cuts at: 0 73 2310
Size: 73 2237
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 713 2310
Size: 713 1597
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 1339 1426 2023 2296 2310
Size: 766 573 87 597 273 14
Fragments arranged by size:
766 597 573 273 87 14
Bpu10I CC'TnA_GC
Cuts at: 0 422 2077 2310
Size: 422 1655 233
Fragments arranged by size:
1655 422 233
BsaI GGTCTCn'nnnn_
Cuts at: 0 1242 2310
Size: 1242 1068
BsaWI w'CCGG_w
Cuts at: 0 1053 1163 2310
Size: 1053 110 1147
Fragments arranged by size:
1147 1053 110
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 966 996 1312 1342 1613 1643 2137 2167
Size: 966 30 316 30 271 30 494 30
Cuts at: 2167 2310
Size: 143
Fragments arranged by size:
966 494 316 271 143 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 262 818 1569 2056 2310
Size: 262 556 751 487 254
Fragments arranged by size:
751 556 487 262 254
BseYI C'CCAG_C
Cuts at: 0 604 835 930 2310
Size: 604 231 95 1380
Fragments arranged by size:
1380 604 231 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 343 2015 2310
Size: 140 203 1672 295
Fragments arranged by size:
1672 295 203 140
BsiHKAI G_wGCw'C
Cuts at: 0 792 810 963 1412 2039 2310
Size: 792 18 153 449 627 271
Fragments arranged by size:
792 627 449 271 153 18
Bsp1286I G_dGCh'C
Cuts at: 0 792 810 963 1412 1441 2039 2310
Size: 792 18 153 449 29 598 271
Fragments arranged by size:
792 598 449 271 153 29 18
BspHI T'CATG_A
Cuts at: 0 1838 2310
Size: 1838 472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1025 1662 2310
Size: 1025 637 648
Fragments arranged by size:
1025 648 637
BsrDI GCAATG_nn'
Cuts at: 0 213 2310
Size: 213 2097
BsrFI r'CCGG_y
Cuts at: 0 2255 2310
Size: 2255 55
BsrGI T'GTAC_A
Cuts at: 0 1752 2310
Size: 1752 558
BssSI C'ACGA_G
Cuts at: 0 2165 2310
Size: 2165 145
BstEII G'GTnAC_C
Cuts at: 0 238 739 2310
Size: 238 501 1571
Fragments arranged by size:
1571 501 238
BstYI r'GATC_y
Cuts at: 0 300 382 1030 1194 2310
Size: 300 82 648 164 1116
Fragments arranged by size:
1116 648 300 164 82
Bsu36I CC'TnA_GG
Cuts at: 0 1391 2310
Size: 1391 919
BtgI C'CryG_G
Cuts at: 0 2082 2310
Size: 2082 228
BtsI GCAGTG_nn'
Cuts at: 0 326 412 720 2027 2059 2310
Size: 326 86 308 1307 32 251
Fragments arranged by size:
1307 326 308 251 86 32
EaeI y'GGCC_r
Cuts at: 0 1397 2198 2310
Size: 1397 801 112
Fragments arranged by size:
1397 801 112
EarI CTCTTCn'nnn_
Cuts at: 0 74 732 1640 1774 2310
Size: 74 658 908 134 536
Fragments arranged by size:
908 658 536 134 74
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 766 1339 1358 1426 2023 2296 2310
Size: 407 359 573 19 68 597 273 14
Fragments arranged by size:
597 573 407 359 273 68 19 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1004 2310
Size: 1004 1306
EcoO109I rG'GnC_Cy
Cuts at: 0 470 498 852 1009 1038 2310
Size: 470 28 354 157 29 1272
Fragments arranged by size:
1272 470 354 157 29 28
EcoRI G'AATT_C
Cuts at: 0 363 2310
Size: 363 1947
HaeII r_GCGC'y
Cuts at: 0 156 2310
Size: 156 2154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 612 644 679 711 1158 1190 1222 1254
Size: 612 32 35 32 447 32 32 32
Cuts at: 1254 1312 1344 1613 1645 2310
Size: 58 32 269 32 665
Fragments arranged by size:
665 612 447 269 58 35 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 530 1087 2253 2310
Size: 530 557 1166 57
Fragments arranged by size:
1166 557 530 57
HindIII A'AGCT_T
Cuts at: 0 1378 1450 2310
Size: 1378 72 860
Fragments arranged by size:
1378 860 72
MscI TGG'CCA
Cuts at: 0 1399 2200 2310
Size: 1399 801 110
Fragments arranged by size:
1399 801 110
MslI CAynn'nnrTG
Cuts at: 0 1837 2032 2310
Size: 1837 195 278
Fragments arranged by size:
1837 278 195
MspA1I CmG'CkG
Cuts at: 0 1221 1333 1828 2310
Size: 1221 112 495 482
Fragments arranged by size:
1221 495 482 112
NdeI CA'TA_TG
Cuts at: 0 1200 2310
Size: 1200 1110
NsiI A_TGCA'T
Cuts at: 0 54 2310
Size: 54 2256
NspI r_CATG'y
Cuts at: 0 1695 2310
Size: 1695 615
PasI CC'CwG_GG
Cuts at: 0 477 2310
Size: 477 1833
PflMI CCAn_nnn'nTGG
Cuts at: 0 374 2310
Size: 374 1936
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2137 2169 2310
Size: 2137 32 141
Fragments arranged by size:
2137 141 32
PpuMI rG'GwC_Cy
Cuts at: 0 498 852 1009 2310
Size: 498 354 157 1301
Fragments arranged by size:
1301 498 354 157
PshAI GACnn'nnGTC
Cuts at: 0 828 2310
Size: 828 1482
PsiI TTA'TAA
Cuts at: 0 1573 2310
Size: 1573 737
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 500 532 2310
Size: 500 32 1778
Fragments arranged by size:
1778 500 32
PstI C_TGCA'G
Cuts at: 0 715 2291 2310
Size: 715 1576 19
Fragments arranged by size:
1576 715 19
PvuII CAG'CTG
Cuts at: 0 1333 1828 2310
Size: 1333 495 482
Fragments arranged by size:
1333 495 482
SalI G'TCGA_C
Cuts at: 0 1085 2310
Size: 1085 1225
SanDI GG'GwC_CC
Cuts at: 0 852 2310
Size: 852 1458
SapI GCTCTTCn'nnn_
Cuts at: 0 1640 2310
Size: 1640 670
SfcI C'TryA_G
Cuts at: 0 434 711 814 1587 2095 2114 2287 2310
Size: 434 277 103 773 508 19 173 23
Fragments arranged by size:
773 508 434 277 173 103 23 19
SmlI C'TyrA_G
Cuts at: 0 1704 2310
Size: 1704 606
StuI AGG'CCT
Cuts at: 0 1734 2310
Size: 1734 576
StyI C'CwwG_G
Cuts at: 0 520 2310
Size: 520 1790
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 226 628 2310
Size: 226 402 1682
Fragments arranged by size:
1682 402 226
TatI w'GTAC_w
Cuts at: 0 1752 2310
Size: 1752 558
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 926 958 2310
Size: 926 32 1352
Fragments arranged by size:
1352 926 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2047 2310
Size: 2047 263
Enzymes that do cut:
AarI AccI AcuI AflII AleI AloI AlwNI ApoI
BamHI BanI BanII BbsI BciVI BclI BfrBI BglI
BglII BmrI BplI BpmI Bpu10I BsaI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrGI BssSI BstEII BstYI Bsu36I BtgI BtsI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
HincII HindIII MscI MslI MspA1I NdeI NsiI NspI
PasI PflMI PpiI PpuMI PshAI PsiI PsrI PstI
PvuII SalI SanDI SapI SfcI SmlI StuI StyI
TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AlfI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BbvCI BcgI BlpI Bme1580I BmgBI BmtI BpuEI
BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BssHII BstAPI BstBI BstXI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRV
FalI FseI FspI FspAI HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PciI PfoI PmeI PmlI PspOMI PspXI
PvuI RsrII SacI SacII SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI