Restriction map for
pFN21AE0513
(Linear) (Six-base) MAPSORT of: KIEE0513.seq Check: 6460 from: 1 to: 993
KIEE0513.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AatII G_ACGT'C
Cuts at: 0 590 993
Size: 590 403
AccI GT'mk_AC
Cuts at: 0 412 993
Size: 412 581
AclI AA'CG_TT
Cuts at: 0 476 993
Size: 476 517
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 865 902 993
Size: 541 324 37 91
Fragments arranged by size:
541 324 91 37
AfeI AGC'GCT
Cuts at: 0 445 993
Size: 445 548
AflIII A'CryG_T
Cuts at: 0 212 993
Size: 212 781
AhdI GACnn_n'nnGTC
Cuts at: 0 408 993
Size: 408 585
AleI CACnn'nnGTG
Cuts at: 0 322 993
Size: 322 671
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 199 231 993
Size: 27 172 32 762
Fragments arranged by size:
762 172 32 27
AlwNI CAG_nnn'CTG
Cuts at: 0 252 669 699 993
Size: 252 417 30 294
Fragments arranged by size:
417 294 252 30
ApoI r'AATT_y
Cuts at: 0 124 778 993
Size: 124 654 215
Fragments arranged by size:
654 215 124
AvaI C'yCGr_G
Cuts at: 0 178 993
Size: 178 815
BanII G_rGCy'C
Cuts at: 0 53 71 227 987 993
Size: 53 18 156 760 6
Fragments arranged by size:
760 156 53 18 6
BlpI GC'TnA_GC
Cuts at: 0 862 993
Size: 862 131
BmrI ACTGGGnnnn_n'
Cuts at: 0 702 993
Size: 702 291
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 87 993
Size: 87 906
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 349 376 856 993
Size: 223 126 27 480 137
Fragments arranged by size:
480 223 137 126 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 993
Size: 247 746
BsaHI Gr'CG_yC
Cuts at: 0 587 973 993
Size: 587 386 20
Fragments arranged by size:
587 386 20
BsaWI w'CCGG_w
Cuts at: 0 341 615 993
Size: 341 274 378
Fragments arranged by size:
378 341 274
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1 25 31 199 229 993
Size: 1 24 6 168 30 764
Fragments arranged by size:
764 168 30 24 6 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 79 220 373 993
Size: 76 3 141 153 620
Fragments arranged by size:
620 153 141 76 3
BseYI C'CCAG_C
Cuts at: 0 54 993
Size: 54 939
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 712 751 799 993
Size: 403 309 39 48 194
Fragments arranged by size:
403 309 194 48 39
BsiHKAI G_wGCw'C
Cuts at: 0 71 185 508 913 926 993
Size: 71 114 323 405 13 67
Fragments arranged by size:
405 323 114 71 67 13
Bsp1286I G_dGCh'C
Cuts at: 0 53 71 185 227 508 913 926 987
Size: 53 18 114 42 281 405 13 61
Cuts at: 987 993
Size: 6
Fragments arranged by size:
405 281 114 61 53 42 18 13
6
BspEI T'CCGG_A
Cuts at: 0 615 993
Size: 615 378
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 32 104 993
Size: 32 72 889
Fragments arranged by size:
889 72 32
BstXI CCAn_nnnn'nTGG
Cuts at: 0 45 993
Size: 45 948
BtgI C'CryG_G
Cuts at: 0 978 993
Size: 978 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 267 993
Size: 267 726
BtsI GCAGTG_nn'
Cuts at: 0 638 993
Size: 638 355
DraIII CAC_nnn'GTG
Cuts at: 0 189 993
Size: 189 804
EaeI y'GGCC_r
Cuts at: 0 437 993
Size: 437 556
EarI CTCTTCn'nnn_
Cuts at: 0 176 993
Size: 176 817
EcoICRI GAG'CTC
Cuts at: 0 69 993
Size: 69 924
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 349 376 541 856 865 902 993
Size: 223 126 27 165 315 9 37 91
Fragments arranged by size:
315 223 165 126 91 37 27 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 205 993
Size: 205 788
EcoO109I rG'GnC_Cy
Cuts at: 0 117 402 855 993
Size: 117 285 453 138
Fragments arranged by size:
453 285 138 117
EcoRI G'AATT_C
Cuts at: 0 778 993
Size: 778 215
EcoRV GAT'ATC
Cuts at: 0 888 993
Size: 888 105
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 606 993
Size: 606 387
HaeII r_GCGC'y
Cuts at: 0 262 447 835 993
Size: 262 185 388 158
Fragments arranged by size:
388 262 185 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 394 426 993
Size: 394 32 567
Fragments arranged by size:
567 394 32
HincII GTy'rAC
Cuts at: 0 660 993
Size: 660 333
MscI TGG'CCA
Cuts at: 0 439 993
Size: 439 554
MslI CAynn'nnrTG
Cuts at: 0 322 993
Size: 322 671
MspA1I CmG'CkG
Cuts at: 0 58 354 866 993
Size: 58 296 512 127
Fragments arranged by size:
512 296 127 58
NcoI C'CATG_G
Cuts at: 0 978 993
Size: 978 15
NspI r_CATG'y
Cuts at: 0 216 993
Size: 216 777
PciI A'CATG_T
Cuts at: 0 212 993
Size: 212 781
PflMI CCAn_nnn'nTGG
Cuts at: 0 252 669 993
Size: 252 417 324
Fragments arranged by size:
417 324 252
PfoI T'CCnGG_A
Cuts at: 0 109 993
Size: 109 884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 199 231 993
Size: 27 172 32 762
Fragments arranged by size:
762 172 32 27
PpuMI rG'GwC_Cy
Cuts at: 0 117 402 993
Size: 117 285 591
Fragments arranged by size:
591 285 117
PstI C_TGCA'G
Cuts at: 0 32 140 353 807 993
Size: 32 108 213 454 186
Fragments arranged by size:
454 213 186 108 32
PvuII CAG'CTG
Cuts at: 0 354 866 993
Size: 354 512 127
Fragments arranged by size:
512 354 127
SacI G_AGCT'C
Cuts at: 0 71 993
Size: 71 922
SfcI C'TryA_G
Cuts at: 0 28 136 349 803 993
Size: 28 108 213 454 190
Fragments arranged by size:
454 213 190 108 28
SmlI C'TyrA_G
Cuts at: 0 262 993
Size: 262 731
StyI C'CwwG_G
Cuts at: 0 38 978 993
Size: 38 940 15
Fragments arranged by size:
940 38 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 117 721 993
Size: 117 604 272
Fragments arranged by size:
604 272 117
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 378 480 715 993
Size: 378 102 235 278
Fragments arranged by size:
378 278 235 102
ZraI GAC'GTC
Cuts at: 0 588 993
Size: 588 405
Enzymes that do cut:
AatII AccI AclI AcuI AfeI AflIII AhdI AleI
AloI AlwNI ApoI AvaI BanII BlpI BmrI BplI
BpmI BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BspMI BstXI BtgI BtgZI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HincII MscI MslI MspA1I
NcoI NspI PciI PflMI PfoI PpiI PpuMI PstI
PvuII SacI SfcI SmlI StyI TaqII XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AflII AgeI AlfI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII Bme1580I
BmgBI BmtI Bpu10I BsaI BsaAI BsaBI BsiEI BsiWI
BsmI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I ClaI
CspCI DraI DrdI EagI EciI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XhoI XmaI XmnI