Restriction map for
pFN21AE0534
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AfaI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbsI BbvCI BciVI BclI BfrI
BlnI BpuAI BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstBI BstUI
BstZ17I ClaI CpoI DraI DraIII
Eam1105I Eco47III EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NcoI NdeI NgoMIV NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccI 418 GTCGAC GT^[AC][GT]AC
AflIII 323 ACGTGT A^C[GA][CT]GT
AlwI 143 GGATC GGATC(4/5)
AocI 310 CCTCAGG CC^T[ACGT]AGG
Asp700I 321 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 555 CTCGAG C^[CT]CG[GA]G
BalI 740 TGGCCA TGG^CCA
BbeI 115 GGCGCC GGCGC^C
BcgI 557 CGAGGCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 13 CTAG C^TAG
BglII 515 AGATCT A^GATCT
BlpI 1217 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1217 GCTCAGC GC^T[ACGT]AGC
BsaI 652 GAGACC GGTCTC(1/5)
BsgI 1226 CTGCAC GTGCAG(16/14)
BsiEI 1010 CGGCCG CG[GA][CT]^CG
BsmAI 652 GAGAC GTCTC(1/5)
BsoBI 555 CTCGAG C^[CT]CG[GA]G
BsrBI 196 GAGCGG CCGCTC(-3/-3)
BsrFI 275 ACCGGC [GA]^CCGG[CT]
BssSI 736 CTCGTG CACGAG(-5/-1)
BstAPI 123 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 641 CCAATGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 515 AGATCT [GA]^GATC[CT]
Bsu36I 310 CCTCAGG CC^T[ACGT]AGG
CelII 1217 GCTCAGC GC^T[ACGT]AGC
Cfr10I 275 ACCGGC [GA]^CCGG[CT]
DrdI 964 GACATCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1010 CGGCCG C^GGCCG
EarI 873 GAAGAG CTCTTC(1/4)
EclXI 1010 CGGCCG C^GGCCG
Eco52I 1010 CGGCCG C^GGCCG
Eco81I 310 CCTCAGG CC^T[ACGT]AGG
HgaI 354 GACGC GACGC(5/10)
HincII 418 GTCGAC GT[CT]^[GA]AC
HindII 418 GTCGAC GT[CT]^[GA]AC
KasI 115 GGCGCC G^GCGCC
Ksp632I 873 GAAGAG CTCTTC(1/4)
MaeI 13 CTAG C^TAG
MaeII 323 ACGT A^CGT
MflI 515 AGATCT [GA]^GATC[CT]
MluNI 740 TGGCCA TGG^CCA
MscI 740 TGGCCA TGG^CCA
MseI 674 TTAA T^TAA
MslI 1265 CACCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 862 CAGCTG C[AC]G^C[GT]G
NarI 115 GGCGCC GG^CGCC
NheI 12 GCTAGC G^CTAGC
NlaIII 30 CATG CATG^
PaeR7I 555 CTCGAG C^TCGAG
PvuII 862 CAGCTG CAG^CTG
SacI 1215 GAGCTC GAGCT^C
SalI 418 GTCGAC G^TCGAC
SapI 873 GAAGAGC GCTCTTC(1/4)
SfoI 115 GGCGCC GGC^GCC
TfiI 953 GAATC G^A[AT]TC
Tru9I 674 TTAA T^TAA
Tsp509I 698 AATT ^AATT
XhoI 555 CTCGAG C^TCGAG
XhoII 515 AGATCT [GA]^GATC[CT]
XmnI 321 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcyI 115 GGCGCC G[GA]^CG[CT]C
354 GACGCC
AspHI 385 GAGCAC G[AT]GC[AT]^C
1215 GAGCTC
BglI 552 GCCCTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1189 GCCCGACTGGC
BsaHI 115 GGCGCC G[GA]^CG[CT]C
354 GACGCC
BseRI 172 CTCCTC GAGGAG(10/8)
1123 GAGGAG
BsiHKAI 385 GAGCAC G[AT]GC[AT]^C
1215 GAGCTC
BspMI 1067 ACCTGC ACCTGC(4/8)
1224 ACCTGC
BstEII 336 GGTGACC G^GT[ACGT]ACC
1050 GGTCACC
BstPI 336 GGTGACC G^GT[ACGT]ACC
1050 GGTCACC
CfoI 116 GCGC GCG^C
169 GCGC
EcoO65I 336 GGTGACC G^GT[ACGT]ACC
1050 GGTCACC
HaeII 115 GGCGCC [GA]GCGC^[CT]
168 GGCGCT
HhaI 116 GCGC GCG^C
169 GCGC
Hin1I 115 GGCGCC G[GA]^CG[CT]C
354 GACGCC
HinP1I 116 GCGC G^CGC
169 GCGC
MboII 873 GAAGA GAAGA(8/7)
329 TCTTC
PleI 832 GACTC GAGTC(4/5)
1251 GAGTC
SmlI 555 CTCGAG C^T[CT][GA]AG
940 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboI
MnlI MspI MvaI MwoI NciI
NdeII NlaIV PflMI PpuMI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I