Restriction map for
pFN21AE0534
(Linear) (Six-base) MAPSORT of: KIEE0534.seq Check: 5168 from: 1 to: 1323
KIEE0534.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1233 1323
Size: 1233 90
AccI GT'mk_AC
Cuts at: 0 419 1323
Size: 419 904
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 600 870 1323
Size: 600 270 453
Fragments arranged by size:
600 453 270
AflIII A'CryG_T
Cuts at: 0 323 1323
Size: 323 1000
AlwNI CAG_nnn'CTG
Cuts at: 0 22 104 129 509 1225 1323
Size: 22 82 25 380 716 98
Fragments arranged by size:
716 380 98 82 25 22
AvaI C'yCGr_G
Cuts at: 0 555 1323
Size: 555 768
BanI G'GyrC_C
Cuts at: 0 115 527 1024 1323
Size: 115 412 497 299
Fragments arranged by size:
497 412 299 115
BanII G_rGCy'C
Cuts at: 0 37 1001 1219 1323
Size: 37 964 218 104
Fragments arranged by size:
964 218 104 37
BbeI G_GCGC'C
Cuts at: 0 119 1323
Size: 119 1204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 546 580 1323
Size: 546 34 743
Fragments arranged by size:
743 546 34
BglI GCCn_nnn'nGGC
Cuts at: 0 558 1195 1323
Size: 558 637 128
Fragments arranged by size:
637 558 128
BglII A'GATC_T
Cuts at: 0 515 1323
Size: 515 808
BlpI GC'TnA_GC
Cuts at: 0 1218 1323
Size: 1218 105
Bme1580I G_kGCm'C
Cuts at: 0 7 532 554 1027 1323
Size: 7 525 22 473 296
Fragments arranged by size:
525 473 296 22 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 207 816 1030 1323
Size: 207 609 214 293
Fragments arranged by size:
609 293 214 207
BmtI G_CTAG'C
Cuts at: 0 16 1323
Size: 16 1307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 770 1128 1323
Size: 540 230 358 195
Fragments arranged by size:
540 358 230 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 925 1323
Size: 925 398
BsaI GGTCTCn'nnnn_
Cuts at: 0 646 1323
Size: 646 677
BsaHI Gr'CG_yC
Cuts at: 0 116 355 1323
Size: 116 239 968
Fragments arranged by size:
968 239 116
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 308 338 1323
Size: 308 30 985
Fragments arranged by size:
985 308 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 1138 1323
Size: 163 975 185
Fragments arranged by size:
975 185 163
BseYI C'CCAG_C
Cuts at: 0 56 413 777 1041 1246 1323
Size: 56 357 364 264 205 77
Fragments arranged by size:
364 357 264 205 77 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1211 1323
Size: 1211 112
BsiEI CG_ry'CG
Cuts at: 0 1013 1323
Size: 1013 310
BsiHKAI G_wGCw'C
Cuts at: 0 389 1219 1323
Size: 389 830 104
Fragments arranged by size:
830 389 104
Bsp1286I G_dGCh'C
Cuts at: 0 7 37 389 532 554 1001 1027 1219
Size: 7 30 352 143 22 447 26 192
Cuts at: 1219 1323
Size: 104
Fragments arranged by size:
447 352 192 143 104 30 26 22
7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1076 1233 1323
Size: 1076 157 90
Fragments arranged by size:
1076 157 90
BsrBI CCG'CTC
Cuts at: 0 198 1323
Size: 198 1125
BsrFI r'CCGG_y
Cuts at: 0 275 1323
Size: 275 1048
BssSI C'ACGA_G
Cuts at: 0 736 1323
Size: 736 587
BstAPI GCAn_nnn'nTGC
Cuts at: 0 129 1323
Size: 129 1194
BstEII G'GTnAC_C
Cuts at: 0 336 1050 1323
Size: 336 714 273
Fragments arranged by size:
714 336 273
BstXI CCAn_nnnn'nTGG
Cuts at: 0 648 1323
Size: 648 675
BstYI r'GATC_y
Cuts at: 0 515 1323
Size: 515 808
Bsu36I CC'TnA_GG
Cuts at: 0 311 1323
Size: 311 1012
BtgI C'CryG_G
Cuts at: 0 459 533 1007 1232 1323
Size: 459 74 474 225 91
Fragments arranged by size:
474 459 225 91 74
BtsI GCAGTG_nn'
Cuts at: 0 678 1238 1323
Size: 678 560 85
Fragments arranged by size:
678 560 85
DrdI GACnn_nn'nnGTC
Cuts at: 0 970 1323
Size: 970 353
EaeI y'GGCC_r
Cuts at: 0 290 740 1010 1196 1235 1323
Size: 290 450 270 186 39 88
Fragments arranged by size:
450 290 270 186 88 39
EagI C'GGCC_G
Cuts at: 0 1010 1323
Size: 1010 313
EarI CTCTTCn'nnn_
Cuts at: 0 868 1323
Size: 868 455
EcoICRI GAG'CTC
Cuts at: 0 1217 1323
Size: 1217 106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 600 770 870 1128 1323
Size: 540 60 170 100 258 195
Fragments arranged by size:
540 258 195 170 100 60
EcoO109I rG'GnC_Cy
Cuts at: 0 201 497 506 560 660 732 755 765
Size: 201 296 9 54 100 72 23 10
Cuts at: 765 1134 1323
Size: 369 189
Fragments arranged by size:
369 296 201 189 100 72 54 23
10 9
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 645 1323
Size: 645 678
HaeII r_GCGC'y
Cuts at: 0 119 172 1323
Size: 119 53 1151
Fragments arranged by size:
1151 119 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 882 914 1323
Size: 376 32 474 32 409
Fragments arranged by size:
474 409 376 32 32
HincII GTy'rAC
Cuts at: 0 420 1323
Size: 420 903
KasI G'GCGC_C
Cuts at: 0 115 1323
Size: 115 1208
MscI TGG'CCA
Cuts at: 0 742 1323
Size: 742 581
MslI CAynn'nnrTG
Cuts at: 0 1269 1323
Size: 1269 54
MspA1I CmG'CkG
Cuts at: 0 864 1323
Size: 864 459
NarI GG'CG_CC
Cuts at: 0 116 1323
Size: 116 1207
NheI G'CTAG_C
Cuts at: 0 12 1323
Size: 12 1311
PasI CC'CwG_GG
Cuts at: 0 40 1323
Size: 40 1283
PflMI CCAn_nnn'nTGG
Cuts at: 0 104 483 902 1323
Size: 104 379 419 421
Fragments arranged by size:
421 419 379 104
PfoI T'CCnGG_A
Cuts at: 0 1312 1323
Size: 1312 11
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 1323
Size: 310 32 981
Fragments arranged by size:
981 310 32
PpuMI rG'GwC_Cy
Cuts at: 0 201 497 506 660 732 755 765 1323
Size: 201 296 9 154 72 23 10 558
Fragments arranged by size:
558 296 201 154 72 23 10 9
PsiI TTA'TAA
Cuts at: 0 74 1323
Size: 74 1249
PspXI vC'TCGA_Gb
Cuts at: 0 555 1323
Size: 555 768
PstI C_TGCA'G
Cuts at: 0 125 137 869 1323
Size: 125 12 732 454
Fragments arranged by size:
732 454 125 12
PvuII CAG'CTG
Cuts at: 0 864 1323
Size: 864 459
SacI G_AGCT'C
Cuts at: 0 1219 1323
Size: 1219 104
SalI G'TCGA_C
Cuts at: 0 418 1323
Size: 418 905
SanDI GG'GwC_CC
Cuts at: 0 497 660 1323
Size: 497 163 663
Fragments arranged by size:
663 497 163
SapI GCTCTTCn'nnn_
Cuts at: 0 868 1323
Size: 868 455
SfcI C'TryA_G
Cuts at: 0 121 133 788 865 1323
Size: 121 12 655 77 458
Fragments arranged by size:
655 458 121 77 12
SfoI GGC'GCC
Cuts at: 0 117 1323
Size: 117 1206
SmlI C'TyrA_G
Cuts at: 0 555 940 1323
Size: 555 385 383
Fragments arranged by size:
555 385 383
StyI C'CwwG_G
Cuts at: 0 647 743 1130 1323
Size: 647 96 387 193
Fragments arranged by size:
647 387 193 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 769 1245 1309 1323
Size: 769 476 64 14
Fragments arranged by size:
769 476 64 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 308 340 1323
Size: 308 32 983
Fragments arranged by size:
983 308 32
XhoI C'TCGA_G
Cuts at: 0 555 1323
Size: 555 768
XmnI GAAnn'nnTTC
Cuts at: 0 325 1323
Size: 325 998
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI AvaI BanI BanII
BbeI BcgI BglI BglII BlpI Bme1580I BmrI BmtI
BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI
EagI EarI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I
HincII KasI MscI MslI MspA1I NarI NheI PasI
PflMI PfoI PpiI PpuMI PsiI PspXI PstI PvuII
SacI SalI SanDI SapI SfcI SfoI SmlI StyI
TaqII TstI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI
BmgBI BplI Bpu10I BsaAI BsaBI BsaWI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BstBI BstZ17I
BtgZI ClaI CspCI DraI DraIII EciI EcoNI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI MmeI NaeI NcoI NdeI NgoMIV NotI NruI
NsiI NspI PacI PciI PmeI PmlI PshAI PspOMI
PsrI PvuI RsrII SacII SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI Tth111I XbaI XcmI XmaI ZraI