(Circular) (Six-base) MAPSORT of: pFN21AB6528.seq Check: 1752 from: 1 to: 5869 pFN21AB6528 5869 bp With 182 enzymes: SgfI * September 11, 2009 17:03 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2436 2436 Size: 5869 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3679 278 Size: 53 83 186 936 2143 2468 Fragments arranged by size: 2468 2143 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3212 1937 Size: 1275 4594 Acc65I G'GTAC_C Cuts at: 3190 3190 Size: 5869 AclI AA'CG_TT Cuts at: 1563 3996 4369 1563 Size: 2433 373 3063 Fragments arranged by size: 3063 2433 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3932 5316 5584 1632 Size: 2300 1384 268 1917 Fragments arranged by size: 2300 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5672 AflIII A'CryG_T Cuts at: 1203 2477 2518 4768 5745 1203 Size: 1274 41 2250 977 1327 Fragments arranged by size: 2250 1327 1274 977 41 AgeI A'CCGG_T Cuts at: 4700 4700 Size: 5869 AhdI GACnn_n'nnGTC Cuts at: 4598 4598 Size: 5869 AleI CACnn'nnGTG Cuts at: 1166 2519 1166 Size: 1353 4516 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3165 1184 Size: 1981 3888 AlwNI CAG_nnn'CTG Cuts at: 2347 3612 5184 2347 Size: 1265 1572 3032 Fragments arranged by size: 3032 1572 1265 ApaLI G'TGCA_C Cuts at: 3926 5082 3926 Size: 1156 4713 ApoI r'AATT_y Cuts at: 1493 2594 2990 3178 3404 3556 5665 1493 Size: 1101 396 188 226 152 2109 1697 Fragments arranged by size: 2109 1697 1101 396 226 188 152 AseI AT'TA_AT Cuts at: 160 1032 4423 160 Size: 872 3391 1606 Fragments arranged by size: 3391 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5869 AvaI C'yCGr_G Cuts at: 1870 1943 2354 3194 1870 Size: 73 411 840 4545 Fragments arranged by size: 4545 840 411 73 BamHI G'GATC_C Cuts at: 3199 3566 3199 Size: 367 5502 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2417 2496 3190 4645 618 Size: 325 217 627 630 79 694 1455 1842 Fragments arranged by size: 1842 1455 694 630 627 325 217 79 BanII G_rGCy'C Cuts at: 1391 2094 3188 1391 Size: 703 1094 4072 Fragments arranged by size: 4072 1094 703 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4898 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2941 2975 4060 4094 1903 Size: 34 1004 34 1085 34 3678 Fragments arranged by size: 3678 1085 1004 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2598 3761 4971 2598 Size: 1163 1210 3496 Fragments arranged by size: 3496 1210 1163 BclI T'GATC_A Cuts at: 1548 3012 1548 Size: 1464 4405 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2027 4480 136 Size: 107 122 71 631 605 355 2453 1525 Fragments arranged by size: 2453 1525 631 605 355 122 107 71 BglII A'GATC_T Cuts at: 5864 5864 Size: 5869 BlpI GC'TnA_GC Cuts at: 3286 3286 Size: 5869 Bme1580I G_kGCm'C Cuts at: 1790 2354 2501 3930 5086 1790 Size: 564 147 1429 1156 2573 Fragments arranged by size: 2573 1429 1156 564 147 BmgBI CAC'GTC Cuts at: 1318 2478 1318 Size: 1160 4709 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2788 3600 4558 Size: 416 53 83 399 24 1371 812 958 Cuts at: 4558 442 Size: 1753 Fragments arranged by size: 1753 1371 958 812 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5869 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2311 2311 Size: 5869 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4529 1239 Size: 3290 2579 Bpu10I CC'TnA_GC Cuts at: 2283 2283 Size: 5869 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2630 3994 4859 5157 2630 Size: 1364 865 298 3342 Fragments arranged by size: 3342 1364 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2901 4532 882 Size: 617 1402 1631 2219 Fragments arranged by size: 2219 1631 1402 617 BsaAI yAC'GTr Cuts at: 493 1204 2521 3660 493 Size: 711 1317 1139 2702 Fragments arranged by size: 2702 1317 1139 711 BsaBI GATnn'nnATC Cuts at: 3017 5863 3017 Size: 2846 3023 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3676 4058 4693 275 Size: 53 83 186 936 2143 382 635 1451 Fragments arranged by size: 2143 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2247 4302 4700 4974 5121 5532 2247 Size: 2055 398 274 147 411 2584 Fragments arranged by size: 2584 2055 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5644 Fragments arranged by size: 5644 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5869 BseYI C'CCAG_C Cuts at: 5072 5568 5072 Size: 496 5373 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5296 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3054 3319 4080 5108 1540 Size: 275 186 1053 265 761 1028 2301 Fragments arranged by size: 2301 1053 1028 761 275 265 186 BsiHKAI G_wGCw'C Cuts at: 2070 2862 3930 4015 5086 2070 Size: 792 1068 85 1071 2853 Fragments arranged by size: 2853 1071 1068 792 85 BsmI GAATG_Cn' Cuts at: 3380 3473 3380 Size: 93 5776 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2094 2354 2501 2862 3188 3930 Size: 399 280 24 260 147 361 326 742 Cuts at: 3930 4015 5086 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 361 326 280 260 147 85 24 BspEI T'CCGG_A Cuts at: 2247 5532 2247 Size: 3285 2584 BspHI T'CATG_A Cuts at: 2094 2307 3758 5488 2094 Size: 213 1451 1730 2475 Fragments arranged by size: 2475 1730 1451 213 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2436 2539 3224 844 Size: 1592 103 685 3489 Fragments arranged by size: 3489 1592 685 103 BsrBI CCG'CTC Cuts at: 1394 1823 3756 1394 Size: 429 1933 3507 Fragments arranged by size: 3507 1933 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4364 4538 65 Size: 1183 3116 174 1396 Fragments arranged by size: 3116 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2405 2414 4513 4700 1700 Size: 110 595 9 2099 187 2869 Fragments arranged by size: 2869 2099 595 187 110 9 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5869 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5869 BssSI C'ACGA_G Cuts at: 3929 4941 3929 Size: 1012 4857 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5869 BstBI TT'CG_AA Cuts at: 4673 4673 Size: 5869 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5869 BstYI r'GATC_y Cuts at: 1972 2250 2674 3199 3566 3951 3968 5420 5506 Size: 278 424 525 367 385 17 1452 86 Cuts at: 5506 5518 5864 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 525 424 385 367 346 278 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2181 2745 513 Size: 552 939 177 564 3637 Fragments arranged by size: 3637 939 564 552 177 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5130 Fragments arranged by size: 5130 683 56 BtsI GCAGTG_nn' Cuts at: 3035 3385 4179 4199 3035 Size: 350 794 20 4705 Fragments arranged by size: 4705 794 350 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5869 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5834 DraI TTT'AAA Cuts at: 2541 3173 2541 Size: 632 5237 DraIII CAC_nnn'GTG Cuts at: 2379 2379 Size: 5869 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2183 3071 4876 809 Size: 507 867 888 1805 1802 Fragments arranged by size: 1805 1802 888 867 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2742 3316 4205 Size: 54 1425 50 275 18 912 574 889 Cuts at: 4205 5834 8 Size: 1629 43 Fragments arranged by size: 1629 1425 912 889 574 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3316 1537 Size: 275 1504 4090 Fragments arranged by size: 4090 1504 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2306 3799 1353 Size: 953 1493 3423 Fragments arranged by size: 3423 1493 953 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2279 2822 4456 4830 4976 387 Size: 1064 138 690 543 1634 374 146 1280 Fragments arranged by size: 1634 1280 1064 690 543 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3932 4529 5316 5584 1239 Size: 393 2300 597 787 268 1524 Fragments arranged by size: 2300 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2344 2344 Size: 5869 EcoRI G'AATT_C Cuts at: 3178 3556 3178 Size: 378 5491 FspI TGC'GCA Cuts at: 4375 4375 Size: 5869 HaeII r_GCGC'y Cuts at: 1678 5016 1678 Size: 3338 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2235 2266 2267 2298 4510 Size: 32 135 32 663 31 1 31 2212 Cuts at: 4510 4542 4584 4616 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2212 663 135 42 32 32 32 32 31 31 1 HincII GTy'rAC Cuts at: 669 1657 1938 2776 3213 3459 669 Size: 988 281 838 437 246 3079 Fragments arranged by size: 3079 988 838 437 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5869 HpaI GTT'AAC Cuts at: 3459 3459 Size: 5869 KpnI G_GTAC'C Cuts at: 3194 3194 Size: 5869 MfeI C'AATT_G Cuts at: 3468 3468 Size: 5869 MluI A'CGCG_T Cuts at: 5745 5745 Size: 5869 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4983 5167 1305 Size: 3678 184 2007 Fragments arranged by size: 3678 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4047 Fragments arranged by size: 4047 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2519 2744 2969 3827 4186 4345 518 Size: 648 1353 225 225 858 359 159 2042 Fragments arranged by size: 2042 1353 858 648 359 225 225 159 MspA1I CmG'CkG Cuts at: 1606 2397 3285 3962 5110 5355 1606 Size: 791 888 677 1148 245 2120 Fragments arranged by size: 2120 1148 888 791 677 245 NaeI GCC'GGC Cuts at: 2407 2416 2407 Size: 9 5860 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4378 Fragments arranged by size: 4378 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5869 NgoMIV G'CCGG_C Cuts at: 2405 2414 2405 Size: 9 5860 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5869 NotI GC'GGCC_GC Cuts at: 3316 3316 Size: 5869 NspI r_CATG'y Cuts at: 1132 1229 3227 4772 1132 Size: 97 1998 1545 2229 Fragments arranged by size: 2229 1998 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5869 PciI A'CATG_T Cuts at: 4768 4768 Size: 5869 PflMI CCAn_nnn'nTGG Cuts at: 1108 2740 1108 Size: 1632 4237 PfoI T'CCnGG_A Cuts at: 2733 2733 Size: 5869 PmeI GTTT'AAAC Cuts at: 3173 3173 Size: 5869 PmlI CAC'GTG Cuts at: 2521 2521 Size: 5869 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3938 3970 5451 5483 3938 Size: 32 1481 32 4324 Fragments arranged by size: 4324 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2204 1613 Size: 591 5278 PsiI TTA'TAA Cuts at: 3439 3439 Size: 5869 PstI C_TGCA'G Cuts at: 830 2551 3221 830 Size: 1721 670 3478 Fragments arranged by size: 3478 1721 670 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5869 PvuII CAG'CTG Cuts at: 2397 2397 Size: 5869 SalI G'TCGA_C Cuts at: 1936 3211 1936 Size: 1275 4594 SbfI CC_TGCA'GG Cuts at: 3221 3221 Size: 5869 ScaI AGT'ACT Cuts at: 2900 4117 5829 2900 Size: 1217 1712 2940 Fragments arranged by size: 2940 1712 1217 SexAI A'CCwGG_T Cuts at: 3165 3165 Size: 5869 SfcI C'TryA_G Cuts at: 826 1045 2547 2902 3217 4352 5033 5224 826 Size: 219 1502 355 315 1135 681 191 1471 Fragments arranged by size: 1502 1471 1135 681 355 315 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5869 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5869 SmaI CCC'GGG Cuts at: 1872 3196 1872 Size: 1324 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2645 3973 4874 5136 820 Size: 197 926 702 1328 901 262 1553 Fragments arranged by size: 1553 1328 926 901 702 262 197 SnaBI TAC'GTA Cuts at: 493 3660 493 Size: 3167 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5869 SphI G_CATG'C Cuts at: 1132 3227 1132 Size: 2095 3774 SspI AAT'ATT Cuts at: 5 52 3793 5 Size: 47 3741 2081 Fragments arranged by size: 3741 2081 47 StuI AGG'CCT Cuts at: 1517 2945 1517 Size: 1428 4441 StyI C'CwwG_G Cuts at: 513 1065 2004 2202 3308 513 Size: 552 939 198 1106 3074 Fragments arranged by size: 3074 1106 939 552 198 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2131 2435 3896 3913 4066 4251 4696 Size: 1255 551 304 1461 17 153 185 445 Cuts at: 4696 325 Size: 1498 Fragments arranged by size: 1498 1461 1255 551 445 304 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2399 2898 4115 Size: 274 80 33 51 1443 422 499 1217 Cuts at: 4115 5827 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1217 499 422 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5829 5861 5829 Size: 32 5837 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5869 XbaI T'CTAG_A Cuts at: 3205 3205 Size: 5869 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5869 XmaI C'CCGG_G Cuts at: 1870 3194 1870 Size: 1324 4545 XmnI GAAnn'nnTTC Cuts at: 3998 3998 Size: 5869 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3677 276 Size: 53 83 186 936 2143 2468 Fragments arranged by size: 2468 2143 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI