Restriction map for
pFN21AB8581
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvrII
BalI BbrPI BbvCI BciVI BfrI
BglI BlnI BsaAI BsaBI BsaI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
EcoT22I FseI HindIII HpaI KpnI
KspI MamI MfeI MluI MluNI
MscI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfaNI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccIII 1550 TCCGGA T^CCGGA
AflIII 1376 ACACGT A^C[GA][CT]GT
AlwNI 917 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AviII 1437 TGCGCA TGC^GCA
BamHI 902 GGATCC G^GATCC
BbsI 1210 GAAGAC GAAGAC(2/6)
BcgI 1088 GCACGCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1007 TGATCA T^GATCA
BfaI 1030 CTAG C^TAG
BglII 1418 AGATCT A^GATCT
BpuAI 1210 GAAGAC GAAGAC(2/6)
BseAI 1550 TCCGGA T^CCGGA
BsgI 1123 CTGCAC GTGCAG(16/14)
BspEI 1550 TCCGGA T^CCGGA
BstEII 1503 GGTGACC G^GT[ACGT]ACC
BstPI 1503 GGTGACC G^GT[ACGT]ACC
BstXI 437 CCACCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 879 TTTAAA TTT^AAA
EarI 1158 GAAGAG CTCTTC(1/4)
EcoO65I 1503 GGTGACC G^GT[ACGT]ACC
EcoRI 531 GAATTC G^AATTC
FbaI 1007 TGATCA T^GATCA
FspI 1437 TGCGCA TGC^GCA
HincII 1072 GTCAAC GT[CT]^[GA]AC
HindII 1072 GTCAAC GT[CT]^[GA]AC
Ksp632I 1158 GAAGAG CTCTTC(1/4)
MaeI 1030 CTAG C^TAG
MroI 1550 TCCGGA T^CCGGA
NaeI 1523 GCCGGC GCC^GGC
NcoI 320 CCATGG C^CATGG
NgoMIV 1523 GCCGGC G^CCGGC
SacI 1174 GAGCTC GAGCT^C
ScaI 646 AGTACT AGT^ACT
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 531 GAATTC [GA]^AATT[CT]
876 GAATTT
AcyI 739 GGCGCC G[GA]^CG[CT]C
891 GGCGCC
ApoI 531 GAATTC [GA]^AATT[CT]
876 GAATTT
BbeI 739 GGCGCC GGCGC^C
891 GGCGCC
BcnI 899 CCGGG CC^[GC]GG
1382 CCGGG
BlpI 521 GCTCAGC GC^T[ACGT]AGC
1218 GCTGAGC
Bpu1102I 521 GCTCAGC GC^T[ACGT]AGC
1218 GCTGAGC
BsaHI 739 GGCGCC G[GA]^CG[CT]C
891 GGCGCC
BsaWI 505 TCCGGT [AT]^CCGG[AT]
1550 TCCGGA
CelII 521 GCTCAGC GC^T[ACGT]AGC
1218 GCTGAGC
DsaI 320 CCATGG C^C[GA][CT]GG
545 CCACGG
EaeI 200 CGGCCA [CT]^GGCC[GA]
575 CGGCCA
EcoNI 82 CCTGCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1053 CCTCCAGCAGG
EcoT14I 320 CCATGG C^C[AT][AT]GG
1508 CCAAGG
HaeII 739 GGCGCC [GA]GCGC^[CT]
891 GGCGCC
HgaI 1321 GACGC GACGC(5/10)
667 GCGTC
Hin1I 739 GGCGCC G[GA]^CG[CT]C
891 GGCGCC
KasI 739 GGCGCC G^GCGCC
891 GGCGCC
MboII 1158 GAAGA GAAGA(8/7)
1210 GAAGA
NarI 739 GGCGCC GG^CGCC
891 GGCGCC
NciI 899 CCGGG CC^[GC]GG
1382 CCGGG
SfoI 739 GGCGCC GGC^GCC
891 GGCGCC
StyI 320 CCATGG C^C[AT][AT]GG
1508 CCAAGG
XcmI 311 CCACCCCAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
434 CCACCACCTCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
ApaI AspHI AvaII BanI BanII
BbvI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI SmlI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII