(Circular) (Six-base) MAPSORT of: pFN21AE0104.seq Check: 6192 from: 1 to: 6091 pFN21AE0104 6091 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3901 278 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3221 3434 1937 Size: 1284 213 4594 Fragments arranged by size: 4594 1284 213 Acc65I G'GTAC_C Cuts at: 3412 3412 Size: 6091 AclI AA'CG_TT Cuts at: 1563 4218 4591 1563 Size: 2655 373 3063 Fragments arranged by size: 3063 2655 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2048 2088 2736 4154 5538 5806 1632 Size: 416 40 648 1418 1384 268 1917 Fragments arranged by size: 1917 1418 1384 648 416 268 40 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 4990 5967 1203 Size: 3787 977 1327 Fragments arranged by size: 3787 1327 977 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 4820 4820 Size: 6091 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6091 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6091 AlwNI CAG_nnn'CTG Cuts at: 2066 3286 3834 5406 2066 Size: 1220 548 1572 2751 Fragments arranged by size: 2751 1572 1220 548 ApaLI G'TGCA_C Cuts at: 4148 5304 4148 Size: 1156 4935 ApoI r'AATT_y Cuts at: 1493 2720 3400 3626 3778 5887 1493 Size: 1227 680 226 152 2109 1697 Fragments arranged by size: 2109 1697 1227 680 226 152 AseI AT'TA_AT Cuts at: 160 1032 3356 4645 160 Size: 872 2324 1289 1606 Fragments arranged by size: 2324 1606 1289 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 2653 3416 1870 Size: 73 710 763 4545 Fragments arranged by size: 4545 763 710 73 BamHI G'GATC_C Cuts at: 3421 3788 3421 Size: 367 5724 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2175 3412 4867 618 Size: 325 217 627 388 1237 1455 1842 Fragments arranged by size: 1842 1455 1237 627 388 325 217 BanII G_rGCy'C Cuts at: 1391 3410 1391 Size: 2019 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2001 2925 928 Size: 971 102 924 4094 Fragments arranged by size: 4094 971 924 102 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4282 4316 1903 Size: 34 2345 34 3678 Fragments arranged by size: 3678 2345 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3983 5193 3983 Size: 1210 4881 BclI T'GATC_A Cuts at: 1548 1548 Size: 6091 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3021 4702 136 Size: 107 122 71 631 605 1349 1681 1525 Fragments arranged by size: 1681 1525 1349 631 605 122 107 71 BglII A'GATC_T Cuts at: 2819 6086 2819 Size: 3267 2824 BlpI GC'TnA_GC Cuts at: 3205 3508 3205 Size: 303 5788 Bme1580I G_kGCm'C Cuts at: 1790 2178 4152 5308 1790 Size: 388 1974 1156 2573 Fragments arranged by size: 2573 1974 1156 388 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6091 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3822 4780 442 Size: 416 53 83 399 24 2405 958 1753 Fragments arranged by size: 2405 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2426 1056 Size: 1370 4721 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3102 4751 1239 Size: 1863 1649 2579 Fragments arranged by size: 2579 1863 1649 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2222 2796 4216 5081 5379 2222 Size: 574 1420 865 298 2934 Fragments arranged by size: 2934 1420 865 574 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4754 882 Size: 617 3255 2219 Fragments arranged by size: 3255 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3882 493 Size: 711 2678 2702 Fragments arranged by size: 2702 2678 711 BsaBI GATnn'nnATC Cuts at: 2472 6085 2472 Size: 3613 2478 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2940 3898 4280 4915 Size: 53 83 186 936 1407 958 382 635 Cuts at: 4915 275 Size: 1451 Fragments arranged by size: 1451 1407 958 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2385 4524 4922 5196 5343 5754 2385 Size: 2139 398 274 147 411 2722 Fragments arranged by size: 2722 2139 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3123 3153 1178 Size: 30 165 30 1720 30 4116 Fragments arranged by size: 4116 1720 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2675 1186 Size: 1489 4602 BseYI C'CCAG_C Cuts at: 2571 2798 5294 5790 2571 Size: 227 2496 496 2872 Fragments arranged by size: 2872 2496 496 227 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2232 1163 Size: 573 496 5022 Fragments arranged by size: 5022 573 496 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3541 4302 5330 1540 Size: 275 186 1540 761 1028 2301 Fragments arranged by size: 2301 1540 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4152 4237 5308 4152 Size: 85 1071 4935 Fragments arranged by size: 4935 1071 85 BsmI GAATG_Cn' Cuts at: 3317 3602 3695 3317 Size: 285 93 5713 Fragments arranged by size: 5713 285 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2178 3410 4152 4237 5308 1391 Size: 399 388 1232 742 85 1071 2174 Fragments arranged by size: 2174 1232 1071 742 399 388 85 BspEI T'CCGG_A Cuts at: 5754 5754 Size: 6091 BspHI T'CATG_A Cuts at: 3980 5710 3980 Size: 1730 4361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3446 844 Size: 2602 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3978 1394 Size: 429 2155 3507 Fragments arranged by size: 3507 2155 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4586 4760 65 Size: 1183 3338 174 1396 Fragments arranged by size: 3338 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4735 4922 1700 Size: 110 2925 187 2869 Fragments arranged by size: 2925 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6091 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6091 BssSI C'ACGA_G Cuts at: 4151 5163 4151 Size: 1012 5079 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6091 BstBI TT'CG_AA Cuts at: 4895 4895 Size: 6091 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6091 BstYI r'GATC_y Cuts at: 1972 2701 2819 3421 3788 4173 4190 5642 5728 Size: 729 118 602 367 385 17 1452 86 Cuts at: 5728 5740 6086 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 729 602 385 367 346 118 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3170 513 Size: 552 2105 3434 Fragments arranged by size: 3434 2105 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5352 Fragments arranged by size: 5352 683 56 BtsI GCAGTG_nn' Cuts at: 3222 3607 4401 4421 3222 Size: 385 794 20 4892 Fragments arranged by size: 4892 794 385 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6091 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 2472 2913 3395 2472 Size: 441 482 5168 Fragments arranged by size: 5168 482 441 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5098 809 Size: 507 3782 1802 Fragments arranged by size: 3782 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3142 3173 3538 Size: 54 1425 50 275 18 1312 31 365 Cuts at: 3538 4427 6056 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1312 889 365 275 54 50 43 31 18 EagI C'GGCC_G Cuts at: 1537 1812 3538 1537 Size: 275 1726 4090 Fragments arranged by size: 4090 1726 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2121 3114 4021 1353 Size: 768 993 907 3423 Fragments arranged by size: 3423 993 907 768 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4678 5052 5198 387 Size: 1064 138 3089 374 146 1280 Fragments arranged by size: 3089 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2088 2736 3102 4154 4751 5538 Size: 393 416 40 648 366 1052 597 787 Cuts at: 5538 5806 1239 Size: 268 1524 Fragments arranged by size: 1524 1052 787 648 597 416 393 366 268 40 EcoO109I rG'GnC_Cy Cuts at: 3283 3283 Size: 6091 EcoRI G'AATT_C Cuts at: 2720 3400 3778 2720 Size: 680 378 5033 Fragments arranged by size: 5033 680 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2546 2605 2546 Size: 59 6032 FspI TGC'GCA Cuts at: 4597 4597 Size: 6091 HaeII r_GCGC'y Cuts at: 1678 5238 1678 Size: 3560 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2211 2243 3267 3299 4732 Size: 32 135 32 639 32 1024 32 1433 Cuts at: 4732 4764 4806 4838 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1433 1024 639 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2088 3167 3238 3435 3681 669 Size: 988 281 150 1079 71 197 246 3079 Fragments arranged by size: 3079 1079 988 281 246 197 150 71 HindIII A'AGCT_T Cuts at: 748 748 Size: 6091 HpaI GTT'AAC Cuts at: 2088 3681 2088 Size: 1593 4498 KpnI G_GTAC'C Cuts at: 3416 3416 Size: 6091 MfeI C'AATT_G Cuts at: 2223 2780 3690 2223 Size: 557 910 4624 Fragments arranged by size: 4624 910 557 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2536 5205 5389 1305 Size: 1231 2669 184 2007 Fragments arranged by size: 2669 2007 1231 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3144 3175 10 Size: 54 1425 343 1312 31 2926 Fragments arranged by size: 2926 1425 1312 343 54 31 MslI CAynn'nnrTG Cuts at: 518 1166 3000 4049 4408 4567 518 Size: 648 1834 1049 359 159 2042 Fragments arranged by size: 2042 1834 1049 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2063 2539 3264 3507 4184 5332 5577 1606 Size: 457 476 725 243 677 1148 245 2120 Fragments arranged by size: 2120 1148 725 677 476 457 245 243 NcoI C'CATG_G Cuts at: 513 1065 3170 513 Size: 552 2105 3434 Fragments arranged by size: 3434 2105 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NheI G'CTAG_C Cuts at: 1052 2422 1052 Size: 1370 4721 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 3052 3449 4994 1132 Size: 97 1823 397 1545 2229 Fragments arranged by size: 2229 1823 1545 397 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6091 PciI A'CATG_T Cuts at: 4990 4990 Size: 6091 PflMI CCAn_nnn'nTGG Cuts at: 1108 2864 1108 Size: 1756 4335 PfoI T'CCnGG_A Cuts at: 3285 3285 Size: 6091 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5673 5705 4160 Size: 32 1481 32 4546 Fragments arranged by size: 4546 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3283 3283 Size: 6091 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6091 PsiI TTA'TAA Cuts at: 2277 3661 2277 Size: 1384 4707 PstI C_TGCA'G Cuts at: 830 3443 830 Size: 2613 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 PvuII CAG'CTG Cuts at: 2063 2539 2063 Size: 476 5615 SalI G'TCGA_C Cuts at: 1936 3433 1936 Size: 1497 4594 SbfI CC_TGCA'GG Cuts at: 3443 3443 Size: 6091 ScaI AGT'ACT Cuts at: 4339 6051 4339 Size: 1712 4379 SfcI C'TryA_G Cuts at: 826 1045 3222 3439 4574 5255 5446 826 Size: 219 2177 217 1135 681 191 1471 Fragments arranged by size: 2177 1471 1135 681 219 217 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 3418 1872 Size: 1546 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2201 2811 4195 5096 5358 820 Size: 197 926 258 610 1384 901 262 1553 Fragments arranged by size: 1553 1384 926 901 610 262 258 197 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 3449 1132 Size: 2317 3774 SspI AAT'ATT Cuts at: 5 52 4015 5 Size: 47 3963 2081 Fragments arranged by size: 3963 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6091 StyI C'CwwG_G Cuts at: 513 1065 2324 2478 3170 3530 513 Size: 552 1259 154 692 360 3074 Fragments arranged by size: 3074 1259 692 552 360 154 SwaI ATTT'AAAT Cuts at: 2472 2472 Size: 6091 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2193 4118 4135 4288 4473 4918 325 Size: 1255 613 1925 17 153 185 445 1498 Fragments arranged by size: 1925 1498 1255 613 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4337 6049 96 Size: 274 80 33 51 1443 2360 1712 138 Fragments arranged by size: 2360 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6051 6083 6051 Size: 32 6059 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6091 XbaI T'CTAG_A Cuts at: 3427 3427 Size: 6091 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 3416 1870 Size: 1546 4545 XmnI GAAnn'nnTTC Cuts at: 4220 4220 Size: 6091 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3899 276 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI