Restriction map for pFN21AE0431
 (Circular) (Six-base) MAPSORT of: pFN21AE0431.seq  Check: 2605  from: 1  to: 5713



pFN21AE0431  5713 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:47  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3523     278 
   Size:         53      83     186     936    1987    2468

  Fragments arranged by size: 

                2468    1987     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2462    3056    1937 
   Size:        525     594    4594

  Fragments arranged by size: 

                4594     594     525
 
Acc65I G'GTAC_C

Cuts at:   3034    3034 
   Size:       5713
 
AclI AA'CG_TT

Cuts at:   1563    3840    4213    1563 
   Size:       2277     373    3063

  Fragments arranged by size: 

                3063    2277     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2237    3776    5160    5428    1632 
   Size:        605    1539    1384     268    1917

  Fragments arranged by size: 

                1917    1539    1384     605     268
 
AflII C'TTAA_G

Cuts at:    820    1017    2848     820 
   Size:        197    1831    3685

  Fragments arranged by size: 

                3685    1831     197
 
AflIII A'CryG_T

Cuts at:   1203    2318    4612    5589    1203 
   Size:       1115    2294     977    1327

  Fragments arranged by size: 

                2294    1327    1115     977
 
AgeI A'CCGG_T

Cuts at:   4544    4544 
   Size:       5713
 
AhdI GACnn_n'nnGTC

Cuts at:   4442    4442 
   Size:       5713
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5713
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5713
 
AlwNI CAG_nnn'CTG

Cuts at:   2534    3456    5028    2534 
   Size:        922    1572    3219

  Fragments arranged by size: 

                3219    1572     922
 
ApaI G_GGCC'C

Cuts at:   2014    2014 
   Size:       5713
 
ApaLI G'TGCA_C

Cuts at:   3770    4926    3770 
   Size:       1156    4557
 
ApoI r'AATT_y

Cuts at:   1493    2165    2563    3022    3248    3400    5509    1493 
   Size:        672     398     459     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     672     459     398     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4267     160 
   Size:        872    3235    1606

  Fragments arranged by size: 

                3235    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5713
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3038    1870 
   Size:         73    1095    4545

  Fragments arranged by size: 

                4545    1095      73
 
BamHI G'GATC_C

Cuts at:   3043    3410    3043 
   Size:        367    5346
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2768    3034    4489     618 
   Size:        325     217     627     981     266    1455    1842

  Fragments arranged by size: 

                1842    1455     981     627     325     266     217
 
BanII G_rGCy'C

Cuts at:   1391    2014    3032    1391 
   Size:        623    1018    4072

  Fragments arranged by size: 

                4072    1018     623
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2132    2144     928 
   Size:        971     233      12    4497

  Fragments arranged by size: 

                4497     971     233      12
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3904    3938    1903 
   Size:         34    1967      34    3678

  Fragments arranged by size: 

                3678    1967      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3605    4815    3605 
   Size:       1210    4503
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5713
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    2081    4324 
   Size:        107     122      71     631     605     337      72    2243

Cuts at:   4324     136 
   Size:       1525

  Fragments arranged by size: 

                2243    1525     631     605     337     122     107      72
                  71
 
BglII A'GATC_T

Cuts at:   5708    5708 
   Size:       5713
 
BlpI GC'TnA_GC

Cuts at:   3130    3130 
   Size:       5713
 
Bme1580I G_kGCm'C

Cuts at:   1790    2014    3774    4930    1790 
   Size:        224    1760    1156    2573

  Fragments arranged by size: 

                2573    1760    1156     224
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5713
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2721    3444    4402 
   Size:        416      53      83     399      24    1304     723     958

Cuts at:   4402     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1304     958     723     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5713
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4373    1239 
   Size:       3134    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2794    3838    4703    5001    2794 
   Size:       1044     865     298    3506

  Fragments arranged by size: 

                3506    1044     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4376     882 
   Size:        617    2877    2219

  Fragments arranged by size: 

                2877    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3504     493 
   Size:        711    2300    2702

  Fragments arranged by size: 

                2702    2300     711
 
BsaBI GATnn'nnATC

Cuts at:   5707    5707 
   Size:       5713
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3520    3902    4537     275 
   Size:         53      83     186     936    1987     382     635    1451

  Fragments arranged by size: 

                1987    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4146    4544    4818    4965    5376    4146 
   Size:        398     274     147     411    4483

  Fragments arranged by size: 

                4483     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5488

  Fragments arranged by size: 

                5488     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2253    1186 
   Size:       1067    4646
 
BseYI C'CCAG_C

Cuts at:   4916    5412    4916 
   Size:        496    5217
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5140
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2034    3163    3924    4952    1540 
   Size:        275     186      33    1129     761    1028    2301

  Fragments arranged by size: 

                2301    1129    1028     761     275     186      33
 
BsiHKAI G_wGCw'C

Cuts at:   2721    2883    3774    3859    4930    2721 
   Size:        162     891      85    1071    3504

  Fragments arranged by size: 

                3504    1071     891     162      85
 
BsmI GAATG_Cn'

Cuts at:   3224    3317    3224 
   Size:         93    5620
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2014    2721    2883    3032    3774    3859    4930 
   Size:        399     224     707     162     149     742      85    1071

Cuts at:   4930    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     707     399     224     162     149
                  85
 
BspEI T'CCGG_A

Cuts at:   5376    5376 
   Size:       5713
 
BspHI T'CATG_A

Cuts at:   3602    5332    3602 
   Size:       1730    3983
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3068     844 
   Size:       2224    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3600    1394 
   Size:        429    1777    3507

  Fragments arranged by size: 

                3507    1777     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4208    4382      65 
   Size:       1183    2960     174    1396

  Fragments arranged by size: 

                2960    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4357    4544    1700 
   Size:        110    2547     187    2869

  Fragments arranged by size: 

                2869    2547     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5713
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5713
 
BssSI C'ACGA_G

Cuts at:   3773    4785    3773 
   Size:       1012    4701
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5713
 
BstBI TT'CG_AA

Cuts at:   2185    4517    2185 
   Size:       2332    3381
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5713
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2695    2695 
   Size:       5713
 
BstYI r'GATC_y

Cuts at:   1972    3043    3410    3795    3812    5264    5350    5362    5708 
   Size:       1071     367     385      17    1452      86      12     346

Cuts at:   5708    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452    1071     385     367     346      86      17
                  12
 
BstZ17I GTA'TAC

Cuts at:   2463    2463 
   Size:       5713
 
BtgI C'CryG_G

Cuts at:    513    1065    2019    2034     513 
   Size:        552     954      15    4192

  Fragments arranged by size: 

                4192     954     552      15
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2640     487 
   Size:        683      56    1414    3560

  Fragments arranged by size: 

                3560    1414     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3229    4023    4043    3229 
   Size:        794      20    4899

  Fragments arranged by size: 

                4899     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5713
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5678
 
DraI TTT'AAA

Cuts at:   2731    3017    2731 
   Size:        286    5427
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4720     809 
   Size:        507    3404    1802

  Fragments arranged by size: 

                3404    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2031    2868    3160 
   Size:         54    1425      50     275      18     201     837     292

Cuts at:   3160    4049    5678       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     837     292     275     201      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2031    3160    1537 
   Size:        275     219    1129    4090

  Fragments arranged by size: 

                4090    1129     275     219
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2114    2427    3643    1353 
   Size:        761     313    1216    3423

  Fragments arranged by size: 

                3423    1216     761     313
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2149    4300    4674    4820     387 
   Size:       1064     138     560    2151     374     146    1280

  Fragments arranged by size: 

                2151    1280    1064     560     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2237    3776    4373    5160    5428    1239 
   Size:        393     605    1539     597     787     268    1524

  Fragments arranged by size: 

                1539    1524     787     605     597     393     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2119    2119 
   Size:       5713
 
EcoO109I rG'GnC_Cy

Cuts at:   2010    2011    2010 
   Size:          1    5712
 
EcoRI G'AATT_C

Cuts at:   2165    3022    3400    2165 
   Size:        857     378    4478

  Fragments arranged by size: 

                4478     857     378
 
EcoRV GAT'ATC

Cuts at:   2900    2900 
   Size:       5713
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2528    2528 
   Size:       5713
 
FspI TGC'GCA

Cuts at:   4219    4219 
   Size:       5713
 
HaeII r_GCGC'y

Cuts at:   1678    2064    4860    1678 
   Size:        386    2796    2531

  Fragments arranged by size: 

                2796    2531     386
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4354    4386    4428    4460    1373 
   Size:         32     135      32    2782      32      42      32    2626

  Fragments arranged by size: 

                2782    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3057    3303     669 
   Size:        988     281    1119     246    3079

  Fragments arranged by size: 

                3079    1119     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2493     748 
   Size:       1745    3968
 
HpaI GTT'AAC

Cuts at:   3303    3303 
   Size:       5713
 
KpnI G_GTAC'C

Cuts at:   3038    3038 
   Size:       5713
 
MfeI C'AATT_G

Cuts at:   3312    3312 
   Size:       5713
 
MluI A'CGCG_T

Cuts at:   5589    5589 
   Size:       5713
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2605    4827    5011    1305 
   Size:       1300    2222     184    2007

  Fragments arranged by size: 

                2222    2007    1300     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2870      10 
   Size:         54    1425     343    1038    2853

  Fragments arranged by size: 

                2853    1425    1038     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2153    2597    2670    2766    3671    4030    4189 
   Size:        648     987     444      73      96     905     359     159

Cuts at:   4189     518 
   Size:       2042

  Fragments arranged by size: 

                2042     987     905     648     444     359     159      96
                  73
 
MspA1I CmG'CkG

Cuts at:   1606    3129    3806    4954    5199    1606 
   Size:       1523     677    1148     245    2120

  Fragments arranged by size: 

                2120    1523    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2019     513 
   Size:        552     954    4207

  Fragments arranged by size: 

                4207     954     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5713
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5713
 
NotI GC'GGCC_GC

Cuts at:   3160    3160 
   Size:       5713
 
NspI r_CATG'y

Cuts at:   1132    1229    2322    3071    4616    1132 
   Size:         97    1093     749    1545    2229

  Fragments arranged by size: 

                2229    1545    1093     749      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5713
 
PciI A'CATG_T

Cuts at:   2318    4612    2318 
   Size:       2294    3419
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5713
 
PmeI GTTT'AAAC

Cuts at:   3017    3017 
   Size:       5713
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3782    3814    5295    5327    3782 
   Size:         32    1481      32    4168

  Fragments arranged by size: 

                4168    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5713
 
PsiI TTA'TAA

Cuts at:   3283    3283 
   Size:       5713
 
PspOMI G'GGCC_C

Cuts at:   2010    2010 
   Size:       5713
 
PstI C_TGCA'G

Cuts at:    830    3065     830 
   Size:       2235    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5713
 
SalI G'TCGA_C

Cuts at:   1936    3055    1936 
   Size:       1119    4594
 
SbfI CC_TGCA'GG

Cuts at:   3065    3065 
   Size:       5713
 
ScaI AGT'ACT

Cuts at:   3961    5673    3961 
   Size:       1712    4001
 
SfcI C'TryA_G

Cuts at:    826    1045    2427    3061    4196    4877    5068     826 
   Size:        219    1382     634    1135     681     191    1471

  Fragments arranged by size: 

                1471    1382    1135     681     634     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5713
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5713
 
SmaI CCC'GGG

Cuts at:   1872    3040    1872 
   Size:       1168    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2773    2848    3817    4718    4980     820 
   Size:        197     926     830      75     969     901     262    1553

  Fragments arranged by size: 

                1553     969     926     901     830     262     197      75
            
 
SnaBI TAC'GTA

Cuts at:    493    3504     493 
   Size:       3011    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5713
 
SphI G_CATG'C

Cuts at:   1132    3071    1132 
   Size:       1939    3774
 
SspI AAT'ATT

Cuts at:      5      52    2396    2789    3637       5 
   Size:         47    2344     393     848    2081

  Fragments arranged by size: 

                2344    2081     848     393      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5713
 
StyI C'CwwG_G

Cuts at:    513    1065    2019    2839    3152     513 
   Size:        552     954     820     313    3074

  Fragments arranged by size: 

                3074     954     820     552     313
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3740    3757    3910    4095    4540     325 
   Size:       1255    2160      17     153     185     445    1498

  Fragments arranged by size: 

                2160    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2103    2629    2934 
   Size:        274      80      33      51    1443     126     526     305

Cuts at:   2934    2962    3959    5671      96 
   Size:         28     997    1712     138

  Fragments arranged by size: 

                1712    1443     997     526     305     274     138     126
                  80      51      33      28
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5673    5705    5673 
   Size:         32    5681
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5713
 
XbaI T'CTAG_A

Cuts at:   3049    3049 
   Size:       5713
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5713
 
XmaI C'CCGG_G

Cuts at:   1870    3038    1870 
   Size:       1168    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2643    2742    3842    2643 
   Size:         99    1100    4514

  Fragments arranged by size: 

                4514    1100      99
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3521     276 
   Size:         53      83     186     936    1987    2468

  Fragments arranged by size: 

                2468    1987     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI
     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI
   PspOMI     PstI     PvuI     SalI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI   Bsu36I   DraIII  EcoICRI
     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI
     PacI     PfoI     PmlI    PpuMI    PspXI     PsrI    PvuII    RsrII
     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI
     SwaI     XcmI
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