(Circular) (Six-base) MAPSORT of: pFN21AE0431.seq Check: 2605 from: 1 to: 5713 pFN21AE0431 5713 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3523 278 Size: 53 83 186 936 1987 2468 Fragments arranged by size: 2468 1987 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2462 3056 1937 Size: 525 594 4594 Fragments arranged by size: 4594 594 525 Acc65I G'GTAC_C Cuts at: 3034 3034 Size: 5713 AclI AA'CG_TT Cuts at: 1563 3840 4213 1563 Size: 2277 373 3063 Fragments arranged by size: 3063 2277 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2237 3776 5160 5428 1632 Size: 605 1539 1384 268 1917 Fragments arranged by size: 1917 1539 1384 605 268 AflII C'TTAA_G Cuts at: 820 1017 2848 820 Size: 197 1831 3685 Fragments arranged by size: 3685 1831 197 AflIII A'CryG_T Cuts at: 1203 2318 4612 5589 1203 Size: 1115 2294 977 1327 Fragments arranged by size: 2294 1327 1115 977 AgeI A'CCGG_T Cuts at: 4544 4544 Size: 5713 AhdI GACnn_n'nnGTC Cuts at: 4442 4442 Size: 5713 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5713 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5713 AlwNI CAG_nnn'CTG Cuts at: 2534 3456 5028 2534 Size: 922 1572 3219 Fragments arranged by size: 3219 1572 922 ApaI G_GGCC'C Cuts at: 2014 2014 Size: 5713 ApaLI G'TGCA_C Cuts at: 3770 4926 3770 Size: 1156 4557 ApoI r'AATT_y Cuts at: 1493 2165 2563 3022 3248 3400 5509 1493 Size: 672 398 459 226 152 2109 1697 Fragments arranged by size: 2109 1697 672 459 398 226 152 AseI AT'TA_AT Cuts at: 160 1032 4267 160 Size: 872 3235 1606 Fragments arranged by size: 3235 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5713 AvaI C'yCGr_G Cuts at: 1870 1943 3038 1870 Size: 73 1095 4545 Fragments arranged by size: 4545 1095 73 BamHI G'GATC_C Cuts at: 3043 3410 3043 Size: 367 5346 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2768 3034 4489 618 Size: 325 217 627 981 266 1455 1842 Fragments arranged by size: 1842 1455 981 627 325 266 217 BanII G_rGCy'C Cuts at: 1391 2014 3032 1391 Size: 623 1018 4072 Fragments arranged by size: 4072 1018 623 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2132 2144 928 Size: 971 233 12 4497 Fragments arranged by size: 4497 971 233 12 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3904 3938 1903 Size: 34 1967 34 3678 Fragments arranged by size: 3678 1967 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3605 4815 3605 Size: 1210 4503 BclI T'GATC_A Cuts at: 1548 1548 Size: 5713 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2081 4324 Size: 107 122 71 631 605 337 72 2243 Cuts at: 4324 136 Size: 1525 Fragments arranged by size: 2243 1525 631 605 337 122 107 72 71 BglII A'GATC_T Cuts at: 5708 5708 Size: 5713 BlpI GC'TnA_GC Cuts at: 3130 3130 Size: 5713 Bme1580I G_kGCm'C Cuts at: 1790 2014 3774 4930 1790 Size: 224 1760 1156 2573 Fragments arranged by size: 2573 1760 1156 224 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5713 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2721 3444 4402 Size: 416 53 83 399 24 1304 723 958 Cuts at: 4402 442 Size: 1753 Fragments arranged by size: 1753 1304 958 723 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5713 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4373 1239 Size: 3134 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2794 3838 4703 5001 2794 Size: 1044 865 298 3506 Fragments arranged by size: 3506 1044 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4376 882 Size: 617 2877 2219 Fragments arranged by size: 2877 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3504 493 Size: 711 2300 2702 Fragments arranged by size: 2702 2300 711 BsaBI GATnn'nnATC Cuts at: 5707 5707 Size: 5713 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3520 3902 4537 275 Size: 53 83 186 936 1987 382 635 1451 Fragments arranged by size: 1987 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4146 4544 4818 4965 5376 4146 Size: 398 274 147 411 4483 Fragments arranged by size: 4483 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5488 Fragments arranged by size: 5488 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2253 1186 Size: 1067 4646 BseYI C'CCAG_C Cuts at: 4916 5412 4916 Size: 496 5217 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5140 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 3163 3924 4952 1540 Size: 275 186 33 1129 761 1028 2301 Fragments arranged by size: 2301 1129 1028 761 275 186 33 BsiHKAI G_wGCw'C Cuts at: 2721 2883 3774 3859 4930 2721 Size: 162 891 85 1071 3504 Fragments arranged by size: 3504 1071 891 162 85 BsmI GAATG_Cn' Cuts at: 3224 3317 3224 Size: 93 5620 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2014 2721 2883 3032 3774 3859 4930 Size: 399 224 707 162 149 742 85 1071 Cuts at: 4930 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 707 399 224 162 149 85 BspEI T'CCGG_A Cuts at: 5376 5376 Size: 5713 BspHI T'CATG_A Cuts at: 3602 5332 3602 Size: 1730 3983 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3068 844 Size: 2224 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3600 1394 Size: 429 1777 3507 Fragments arranged by size: 3507 1777 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4208 4382 65 Size: 1183 2960 174 1396 Fragments arranged by size: 2960 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4357 4544 1700 Size: 110 2547 187 2869 Fragments arranged by size: 2869 2547 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5713 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5713 BssSI C'ACGA_G Cuts at: 3773 4785 3773 Size: 1012 4701 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5713 BstBI TT'CG_AA Cuts at: 2185 4517 2185 Size: 2332 3381 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5713 BstXI CCAn_nnnn'nTGG Cuts at: 2695 2695 Size: 5713 BstYI r'GATC_y Cuts at: 1972 3043 3410 3795 3812 5264 5350 5362 5708 Size: 1071 367 385 17 1452 86 12 346 Cuts at: 5708 1972 Size: 1977 Fragments arranged by size: 1977 1452 1071 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2463 2463 Size: 5713 BtgI C'CryG_G Cuts at: 513 1065 2019 2034 513 Size: 552 954 15 4192 Fragments arranged by size: 4192 954 552 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2640 487 Size: 683 56 1414 3560 Fragments arranged by size: 3560 1414 683 56 BtsI GCAGTG_nn' Cuts at: 3229 4023 4043 3229 Size: 794 20 4899 Fragments arranged by size: 4899 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5713 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5678 DraI TTT'AAA Cuts at: 2731 3017 2731 Size: 286 5427 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4720 809 Size: 507 3404 1802 Fragments arranged by size: 3404 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2868 3160 Size: 54 1425 50 275 18 201 837 292 Cuts at: 3160 4049 5678 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 837 292 275 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2031 3160 1537 Size: 275 219 1129 4090 Fragments arranged by size: 4090 1129 275 219 EarI CTCTTCn'nnn_ Cuts at: 1353 2114 2427 3643 1353 Size: 761 313 1216 3423 Fragments arranged by size: 3423 1216 761 313 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2149 4300 4674 4820 387 Size: 1064 138 560 2151 374 146 1280 Fragments arranged by size: 2151 1280 1064 560 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 3776 4373 5160 5428 1239 Size: 393 605 1539 597 787 268 1524 Fragments arranged by size: 1539 1524 787 605 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2119 2119 Size: 5713 EcoO109I rG'GnC_Cy Cuts at: 2010 2011 2010 Size: 1 5712 EcoRI G'AATT_C Cuts at: 2165 3022 3400 2165 Size: 857 378 4478 Fragments arranged by size: 4478 857 378 EcoRV GAT'ATC Cuts at: 2900 2900 Size: 5713 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2528 2528 Size: 5713 FspI TGC'GCA Cuts at: 4219 4219 Size: 5713 HaeII r_GCGC'y Cuts at: 1678 2064 4860 1678 Size: 386 2796 2531 Fragments arranged by size: 2796 2531 386 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4354 4386 4428 4460 1373 Size: 32 135 32 2782 32 42 32 2626 Fragments arranged by size: 2782 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3057 3303 669 Size: 988 281 1119 246 3079 Fragments arranged by size: 3079 1119 988 281 246 HindIII A'AGCT_T Cuts at: 748 2493 748 Size: 1745 3968 HpaI GTT'AAC Cuts at: 3303 3303 Size: 5713 KpnI G_GTAC'C Cuts at: 3038 3038 Size: 5713 MfeI C'AATT_G Cuts at: 3312 3312 Size: 5713 MluI A'CGCG_T Cuts at: 5589 5589 Size: 5713 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2605 4827 5011 1305 Size: 1300 2222 184 2007 Fragments arranged by size: 2222 2007 1300 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2870 10 Size: 54 1425 343 1038 2853 Fragments arranged by size: 2853 1425 1038 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2153 2597 2670 2766 3671 4030 4189 Size: 648 987 444 73 96 905 359 159 Cuts at: 4189 518 Size: 2042 Fragments arranged by size: 2042 987 905 648 444 359 159 96 73 MspA1I CmG'CkG Cuts at: 1606 3129 3806 4954 5199 1606 Size: 1523 677 1148 245 2120 Fragments arranged by size: 2120 1523 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2019 513 Size: 552 954 4207 Fragments arranged by size: 4207 954 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5713 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5713 NotI GC'GGCC_GC Cuts at: 3160 3160 Size: 5713 NspI r_CATG'y Cuts at: 1132 1229 2322 3071 4616 1132 Size: 97 1093 749 1545 2229 Fragments arranged by size: 2229 1545 1093 749 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5713 PciI A'CATG_T Cuts at: 2318 4612 2318 Size: 2294 3419 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5713 PmeI GTTT'AAAC Cuts at: 3017 3017 Size: 5713 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3782 3814 5295 5327 3782 Size: 32 1481 32 4168 Fragments arranged by size: 4168 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5713 PsiI TTA'TAA Cuts at: 3283 3283 Size: 5713 PspOMI G'GGCC_C Cuts at: 2010 2010 Size: 5713 PstI C_TGCA'G Cuts at: 830 3065 830 Size: 2235 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5713 SalI G'TCGA_C Cuts at: 1936 3055 1936 Size: 1119 4594 SbfI CC_TGCA'GG Cuts at: 3065 3065 Size: 5713 ScaI AGT'ACT Cuts at: 3961 5673 3961 Size: 1712 4001 SfcI C'TryA_G Cuts at: 826 1045 2427 3061 4196 4877 5068 826 Size: 219 1382 634 1135 681 191 1471 Fragments arranged by size: 1471 1382 1135 681 634 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5713 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5713 SmaI CCC'GGG Cuts at: 1872 3040 1872 Size: 1168 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2773 2848 3817 4718 4980 820 Size: 197 926 830 75 969 901 262 1553 Fragments arranged by size: 1553 969 926 901 830 262 197 75 SnaBI TAC'GTA Cuts at: 493 3504 493 Size: 3011 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5713 SphI G_CATG'C Cuts at: 1132 3071 1132 Size: 1939 3774 SspI AAT'ATT Cuts at: 5 52 2396 2789 3637 5 Size: 47 2344 393 848 2081 Fragments arranged by size: 2344 2081 848 393 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5713 StyI C'CwwG_G Cuts at: 513 1065 2019 2839 3152 513 Size: 552 954 820 313 3074 Fragments arranged by size: 3074 954 820 552 313 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3740 3757 3910 4095 4540 325 Size: 1255 2160 17 153 185 445 1498 Fragments arranged by size: 2160 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2103 2629 2934 Size: 274 80 33 51 1443 126 526 305 Cuts at: 2934 2962 3959 5671 96 Size: 28 997 1712 138 Fragments arranged by size: 1712 1443 997 526 305 274 138 126 80 51 33 28 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5673 5705 5673 Size: 32 5681 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5713 XbaI T'CTAG_A Cuts at: 3049 3049 Size: 5713 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5713 XmaI C'CCGG_G Cuts at: 1870 3038 1870 Size: 1168 4545 XmnI GAAnn'nnTTC Cuts at: 2643 2742 3842 2643 Size: 99 1100 4514 Fragments arranged by size: 4514 1100 99 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3521 276 Size: 53 83 186 936 1987 2468 Fragments arranged by size: 2468 1987 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI