Restriction map for
pFN21AE0431
(Linear) (Six-base) MAPSORT of: KIEE0431.seq Check: 7648 from: 1 to: 1008
KIEE0431.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 0 457 1008
Size: 457 551
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1008
Size: 232 776
AflII C'TTAA_G
Cuts at: 0 843 1008
Size: 843 165
AflIII A'CryG_T
Cuts at: 0 313 1008
Size: 313 695
AlwNI CAG_nnn'CTG
Cuts at: 0 529 1008
Size: 529 479
ApaI G_GGCC'C
Cuts at: 0 9 1008
Size: 9 999
ApoI r'AATT_y
Cuts at: 0 160 558 1008
Size: 160 398 450
Fragments arranged by size:
450 398 160
BanI G'GyrC_C
Cuts at: 0 763 1008
Size: 763 245
BanII G_rGCy'C
Cuts at: 0 9 1008
Size: 9 999
BbsI GAAGACnn'nnnn_
Cuts at: 0 127 139 1008
Size: 127 12 869
Fragments arranged by size:
869 127 12
BglI GCCn_nnn'nGGC
Cuts at: 0 76 1008
Size: 76 932
Bme1580I G_kGCm'C
Cuts at: 0 9 1008
Size: 9 999
BmrI ACTGGGnnnn_n'
Cuts at: 0 716 1008
Size: 716 292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 789 1008
Size: 789 219
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 248 1008
Size: 248 760
BsiEI CG_ry'CG
Cuts at: 0 29 1008
Size: 29 979
BsiHKAI G_wGCw'C
Cuts at: 0 716 878 1008
Size: 716 162 130
Fragments arranged by size:
716 162 130
Bsp1286I G_dGCh'C
Cuts at: 0 9 716 878 1008
Size: 9 707 162 130
Fragments arranged by size:
707 162 130 9
BstBI TT'CG_AA
Cuts at: 0 180 1008
Size: 180 828
BstXI CCAn_nnnn'nTGG
Cuts at: 0 690 1008
Size: 690 318
BstZ17I GTA'TAC
Cuts at: 0 458 1008
Size: 458 550
BtgI C'CryG_G
Cuts at: 0 14 29 1008
Size: 14 15 979
Fragments arranged by size:
979 15 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 635 1008
Size: 635 373
DraI TTT'AAA
Cuts at: 0 726 1008
Size: 726 282
EaeI y'GGCC_r
Cuts at: 0 26 863 1008
Size: 26 837 145
Fragments arranged by size:
837 145 26
EagI C'GGCC_G
Cuts at: 0 26 1008
Size: 26 982
EarI CTCTTCn'nnn_
Cuts at: 0 109 422 1008
Size: 109 313 586
Fragments arranged by size:
586 313 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 144 1008
Size: 144 864
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1008
Size: 232 776
EcoNI CCTnn'n_nnAGG
Cuts at: 0 114 1008
Size: 114 894
EcoO109I rG'GnC_Cy
Cuts at: 0 5 6 1008
Size: 5 1 1002
Fragments arranged by size:
1002 5 1
EcoRI G'AATT_C
Cuts at: 0 160 1008
Size: 160 848
EcoRV GAT'ATC
Cuts at: 0 895 1008
Size: 895 113
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 523 1008
Size: 523 485
HaeII r_GCGC'y
Cuts at: 0 59 1008
Size: 59 949
HindIII A'AGCT_T
Cuts at: 0 488 1008
Size: 488 520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 600 1008
Size: 600 408
MscI TGG'CCA
Cuts at: 0 865 1008
Size: 865 143
MslI CAynn'nnrTG
Cuts at: 0 148 592 665 761 1008
Size: 148 444 73 96 247
Fragments arranged by size:
444 247 148 96 73
NcoI C'CATG_G
Cuts at: 0 14 1008
Size: 14 994
NspI r_CATG'y
Cuts at: 0 317 1008
Size: 317 691
PciI A'CATG_T
Cuts at: 0 313 1008
Size: 313 695
PspOMI G'GGCC_C
Cuts at: 0 5 1008
Size: 5 1003
SfcI C'TryA_G
Cuts at: 0 422 1008
Size: 422 586
SmlI C'TyrA_G
Cuts at: 0 768 843 1008
Size: 768 75 165
Fragments arranged by size:
768 165 75
SspI AAT'ATT
Cuts at: 0 391 784 1008
Size: 391 393 224
Fragments arranged by size:
393 391 224
StyI C'CwwG_G
Cuts at: 0 14 834 1008
Size: 14 820 174
Fragments arranged by size:
820 174 14
TatI w'GTAC_w
Cuts at: 0 98 624 929 957 1008
Size: 98 526 305 28 51
Fragments arranged by size:
526 305 98 51 28
XmnI GAAnn'nnTTC
Cuts at: 0 638 737 1008
Size: 638 99 271
Fragments arranged by size:
638 271 99
Enzymes that do cut:
AccI AcuI AflII AflIII AlwNI ApaI ApoI BanI
BanII BbsI BglI Bme1580I BmrI BpuEI BseRI BsiEI
BsiHKAI Bsp1286I BstBI BstXI BstZ17I BtgI BtgZI DraI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI HaeII HindIII MmeI MscI MslI NcoI
NspI PciI PspOMI SfcI SmlI SspI StyI TatI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BplI BpmI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstYI Bsu36I BtsI
ClaI CspCI DraIII DrdI EcoICRI FseI FspI FspAI
Hin4I HincII HpaI KasI KpnI MfeI MluI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI ZraI