Restriction map for
pFN21AE0147
(Circular) (Six-base) MAPSORT of: pFN21AE0147.seq Check: 8861 from: 1 to: 5317
pFN21AE0147 5317 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3127 278
Size: 53 83 186 936 1591 2468
Fragments arranged by size:
2468 1591 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2660 1937
Size: 723 4594
Acc65I G'GTAC_C
Cuts at: 2638 2638
Size: 5317
AclI AA'CG_TT
Cuts at: 1563 3444 3817 1563
Size: 1881 373 3063
Fragments arranged by size:
3063 1881 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3380 4764 5032 1632
Size: 1748 1384 268 1917
Fragments arranged by size:
1917 1748 1384 268
AfeI AGC'GCT
Cuts at: 2367 2367
Size: 5317
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5120
AflIII A'CryG_T
Cuts at: 1203 4216 5193 1203
Size: 3013 977 1327
Fragments arranged by size:
3013 1327 977
AgeI A'CCGG_T
Cuts at: 4148 4148
Size: 5317
AhdI GACnn_n'nnGTC
Cuts at: 2150 4046 2150
Size: 1896 3421
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5317
AlwNI CAG_nnn'CTG
Cuts at: 2120 2338 3060 4632 2120
Size: 218 722 1572 2805
Fragments arranged by size:
2805 1572 722 218
ApaLI G'TGCA_C
Cuts at: 3374 4530 3374
Size: 1156 4161
ApoI r'AATT_y
Cuts at: 1493 2626 2852 3004 5113 1493
Size: 1133 226 152 2109 1697
Fragments arranged by size:
2109 1697 1133 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3871 160
Size: 872 2839 1606
Fragments arranged by size:
2839 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5317
AvaI C'yCGr_G
Cuts at: 1870 1943 2206 2642 1870
Size: 73 263 436 4545
Fragments arranged by size:
4545 436 263 73
BamHI G'GATC_C
Cuts at: 2647 3014 2647
Size: 367 4950
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2342 2638 4093 618
Size: 325 217 627 555 296 1455 1842
Fragments arranged by size:
1842 1455 627 555 325 296 217
BanII G_rGCy'C
Cuts at: 1391 2454 2636 1391
Size: 1063 182 4072
Fragments arranged by size:
4072 1063 182
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2479 928
Size: 971 580 3766
Fragments arranged by size:
3766 971 580
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3508 3542 1903
Size: 34 1571 34 3678
Fragments arranged by size:
3678 1571 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3209 4419 3209
Size: 1210 4107
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5317
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2358 3928 136
Size: 107 122 71 631 605 686 1570 1525
Fragments arranged by size:
1570 1525 686 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5312 5312
Size: 5317
BlpI GC'TnA_GC
Cuts at: 2429 2734 2429
Size: 305 5012
Bme1580I G_kGCm'C
Cuts at: 1790 2162 2265 2345 3378 4534 1790
Size: 372 103 80 1033 1156 2573
Fragments arranged by size:
2573 1156 1033 372 103 80
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5317
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3048 4006 442
Size: 416 53 83 399 24 1631 958 1753
Fragments arranged by size:
1753 1631 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5317
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2408 2408
Size: 5317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2294 2403 3977 1239
Size: 1055 109 1574 2579
Fragments arranged by size:
2579 1574 1055 109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3442 4307 4605 3442
Size: 865 298 4154
Fragments arranged by size:
4154 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3980 882
Size: 617 2481 2219
Fragments arranged by size:
2481 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2479 3108 493
Size: 711 1275 629 2702
Fragments arranged by size:
2702 1275 711 629
BsaBI GATnn'nnATC
Cuts at: 5311 5311
Size: 5317
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3124 3506 4141 275
Size: 53 83 186 936 1591 382 635 1451
Fragments arranged by size:
1591 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2149 3750 4148 4422 4569 4980 2149
Size: 1601 398 274 147 411 2486
Fragments arranged by size:
2486 1601 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5092
Fragments arranged by size:
5092 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5317
BseYI C'CCAG_C
Cuts at: 2048 2227 4520 5016 2048
Size: 179 2293 496 2349
Fragments arranged by size:
2349 2293 496 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2075 2133 2609 1163
Size: 573 339 58 476 3871
Fragments arranged by size:
3871 573 476 339 58
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2767 3528 4556 1540
Size: 275 186 766 761 1028 2301
Fragments arranged by size:
2301 1028 766 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3378 3463 4534 3378
Size: 85 1071 4161
Fragments arranged by size:
4161 1071 85
BsmI GAATG_Cn'
Cuts at: 2828 2921 2828
Size: 93 5224
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2162 2265 2345 2454 2636 3378 3463
Size: 399 372 103 80 109 182 742 85
Cuts at: 3463 4534 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 372 182 109 103
85 80
BspEI T'CCGG_A
Cuts at: 4980 4980
Size: 5317
BspHI T'CATG_A
Cuts at: 3206 4936 3206
Size: 1730 3587
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2097 2672 844
Size: 1253 575 3489
Fragments arranged by size:
3489 1253 575
BsrBI CCG'CTC
Cuts at: 1394 1823 2082 3204 1394
Size: 429 259 1122 3507
Fragments arranged by size:
3507 1122 429 259
BsrDI GCAATG_nn'
Cuts at: 65 1248 2536 3812 3986 65
Size: 1183 1288 1276 174 1396
Fragments arranged by size:
1396 1288 1276 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2166 3961 4148 1700
Size: 110 356 1795 187 2869
Fragments arranged by size:
2869 1795 356 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5317
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5317
BssSI C'ACGA_G
Cuts at: 3377 4389 3377
Size: 1012 4305
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2120 794
Size: 1326 3991
BstBI TT'CG_AA
Cuts at: 4121 4121
Size: 5317
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5317
BstYI r'GATC_y
Cuts at: 1972 2647 3014 3399 3416 4868 4954 4966 5312
Size: 675 367 385 17 1452 86 12 346
Cuts at: 5312 1972
Size: 1977
Fragments arranged by size:
1977 1452 675 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2139 2139
Size: 5317
BtgI C'CryG_G
Cuts at: 513 1065 2041 513
Size: 552 976 3789
Fragments arranged by size:
3789 976 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4578
Fragments arranged by size:
4578 683 56
BtsI GCAGTG_nn'
Cuts at: 2118 2265 2833 3627 3647 2118
Size: 147 568 794 20 3788
Fragments arranged by size:
3788 794 568 147 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5317
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2518 2553 529
Size: 35 1954 35 3293
Fragments arranged by size:
3293 1954 35 35
DraI TTT'AAA
Cuts at: 2621 2621
Size: 5317
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4324 809
Size: 507 3008 1802
Fragments arranged by size:
3008 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2539 2764 3653
Size: 54 1425 50 275 18 709 225 889
Cuts at: 3653 5282 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 709 275 225 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2764 1537
Size: 275 952 4090
Fragments arranged by size:
4090 952 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2102 2411 3247 1353
Size: 749 309 836 3423
Fragments arranged by size:
3423 836 749 309
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3904 4278 4424 387
Size: 1064 138 2315 374 146 1280
Fragments arranged by size:
2315 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 2403 3380 3977 4764 5032 1239
Size: 393 662 109 977 597 787 268 1524
Fragments arranged by size:
1524 977 787 662 597 393 268 109
EcoO109I rG'GnC_Cy
Cuts at: 2144 2211 2335 2144
Size: 67 124 5126
Fragments arranged by size:
5126 124 67
EcoRI G'AATT_C
Cuts at: 2626 3004 2626
Size: 378 4939
FspI TGC'GCA
Cuts at: 3823 3823
Size: 5317
HaeII r_GCGC'y
Cuts at: 1678 2369 4464 1678
Size: 691 2095 2531
Fragments arranged by size:
2531 2095 691
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2005 2037 2136 2168 3958
Size: 32 135 32 433 32 99 32 1790
Cuts at: 3958 3990 4032 4064 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1790 433 135 99 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2661 2907 669
Size: 988 281 723 246 3079
Fragments arranged by size:
3079 988 723 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5317
HpaI GTT'AAC
Cuts at: 2907 2907
Size: 5317
KpnI G_GTAC'C
Cuts at: 2642 2642
Size: 5317
MfeI C'AATT_G
Cuts at: 2916 2916
Size: 5317
MluI A'CGCG_T
Cuts at: 5193 5193
Size: 5317
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2168 2286 4431 4615 1305
Size: 863 118 2145 184 2007
Fragments arranged by size:
2145 2007 863 184 118
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2541 10
Size: 54 1425 343 709 2786
Fragments arranged by size:
2786 1425 709 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3275 3634 3793 518
Size: 648 2109 359 159 2042
Fragments arranged by size:
2109 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2034 2043 2314 2381 2733 3410 4558 4803
Size: 428 9 271 67 352 677 1148 245
Cuts at: 4803 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 428 352 271 245 67
9
NaeI GCC'GGC
Cuts at: 2168 2168
Size: 5317
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4765
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5317
NgoMIV G'CCGG_C
Cuts at: 2166 2166
Size: 5317
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5317
NotI GC'GGCC_GC
Cuts at: 2764 2764
Size: 5317
NspI r_CATG'y
Cuts at: 1132 1229 2675 4220 1132
Size: 97 1446 1545 2229
Fragments arranged by size:
2229 1545 1446 97
PasI CC'CwG_GG
Cuts at: 1348 2338 1348
Size: 990 4327
PciI A'CATG_T
Cuts at: 4216 4216
Size: 5317
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2338 1108
Size: 1230 4087
PmeI GTTT'AAAC
Cuts at: 2621 2621
Size: 5317
PmlI CAC'GTG
Cuts at: 2479 2479
Size: 5317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2279 2311 3386 3418 4899 4931 2279
Size: 32 1075 32 1481 32 2665
Fragments arranged by size:
2665 1481 1075 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2144 2211 2144
Size: 67 5250
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5317
PsiI TTA'TAA
Cuts at: 2887 2887
Size: 5317
PstI C_TGCA'G
Cuts at: 830 2033 2039 2669 830
Size: 1203 6 630 3478
Fragments arranged by size:
3478 1203 630 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5317
PvuII CAG'CTG
Cuts at: 2034 2381 2034
Size: 347 4970
SacII CC_GC'GG
Cuts at: 2044 2044
Size: 5317
SalI G'TCGA_C
Cuts at: 1936 2659 1936
Size: 723 4594
SanDI GG'GwC_CC
Cuts at: 2211 2211
Size: 5317
SbfI CC_TGCA'GG
Cuts at: 2669 2669
Size: 5317
ScaI AGT'ACT
Cuts at: 3565 5277 3565
Size: 1712 3605
SfcI C'TryA_G
Cuts at: 826 1045 2029 2035 2665 3800 4481 4672 826
Size: 219 984 6 630 1135 681 191 1471
Fragments arranged by size:
1471 1135 984 681 630 219 191 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5317
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5317
SmaI CCC'GGG
Cuts at: 1872 2644 1872
Size: 772 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3421 4322 4584 820
Size: 197 926 1478 901 262 1553
Fragments arranged by size:
1553 1478 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3108 493
Size: 2615 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5317
SphI G_CATG'C
Cuts at: 1132 2675 1132
Size: 1543 3774
SspI AAT'ATT
Cuts at: 5 52 3241 5
Size: 47 3189 2081
Fragments arranged by size:
3189 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5317
StyI C'CwwG_G
Cuts at: 513 1065 2442 2454 2756 513
Size: 552 1377 12 302 3074
Fragments arranged by size:
3074 1377 552 302 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2219 2533 3344 3361 3514 3699 4144
Size: 1255 639 314 811 17 153 185 445
Cuts at: 4144 325
Size: 1498
Fragments arranged by size:
1498 1255 811 639 445 314 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3563 5275 96
Size: 274 80 33 51 1443 1586 1712 138
Fragments arranged by size:
1712 1586 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2051 2083 2508 2540 5277 5309 2051
Size: 32 425 32 2737 32 2059
Fragments arranged by size:
2737 2059 425 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5317
XbaI T'CTAG_A
Cuts at: 2653 2653
Size: 5317
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5317
XmaI C'CCGG_G
Cuts at: 1870 2642 1870
Size: 772 4545
XmnI GAAnn'nnTTC
Cuts at: 3446 3446
Size: 5317
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3125 276
Size: 53 83 186 936 1591 2468
Fragments arranged by size:
2468 1591 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI
SapI SexAI SfiI SfoI SrfI SwaI XcmI