Restriction map for
pFN21AB7803
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BseRI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI EagI EclXI
Eco47III Eco52I Eco81I EcoT22I FbaI
FseI FspI Hin1I HindIII KasI
KpnI KspI MaeI MfeI MluI
MslI MunI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcsI 575 GAATTC [GA]^AATT[CT]
AflIII 325 ACACGT A^C[GA][CT]GT
ApoI 575 GAATTC [GA]^AATT[CT]
AspHI 1319 GAGCAC G[AT]GC[AT]^C
AvaI 142 CCCGAG C^[CT]CG[GA]G
BalI 1424 TGGCCA TGG^CCA
BbvCI 1255 GCTGAGG CCTCAGC(-5/-2)
BmyI 1319 GAGCAC G[AGT]GC[ACT]^C
BsaBI 1101 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 1319 GAGCAC G[AT]GC[AT]^C
BsoBI 142 CCCGAG C^[CT]CG[GA]G
Bsp1286I 1319 GAGCAC G[AGT]GC[ACT]^C
BssSI 371 CTCGTG CACGAG(-5/-1)
BstAPI 102 GCATAGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 1107 CATCC GGATG(2/0)
BstYI 1358 AGATCC [GA]^GATC[CT]
DraI 430 TTTAAA TTT^AAA
DraII 222 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 10 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1456 GACAGCGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 222 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 575 GAATTC G^AATTC
FauI 822 GCGGG CCCGC(4/6)
FokI 1107 CATCC GGATG(9/13)
HaeII 1212 GGCGCT [GA]GCGC^[CT]
HgaI 1116 GACGC GACGC(5/10)
HincII 541 GTTAAC GT[CT]^[GA]AC
HindII 541 GTTAAC GT[CT]^[GA]AC
HpaI 541 GTTAAC GTT^AAC
MamI 1101 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1358 AGATCC [GA]^GATC[CT]
MluNI 1424 TGGCCA TGG^CCA
MscI 1424 TGGCCA TGG^CCA
NdeI 31 CATATG CA^TATG
PpuMI 222 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 912 GACTGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 218 CTGCAG CTGCA^G
SapI 748 GAAGAGC GCTCTTC(1/4)
ScaI 641 AGTACT AGT^ACT
SmlI 637 CTCAAG C^T[CT][GA]AG
XhoII 1358 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 308 TCCGGA T^CCGGA
728 TCCGGA
AhdI 496 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1280 GACAGATCGTC
AlwI 486 GATCC GGATC(4/5)
1359 GATCC
AspEI 496 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1280 GACAGATCGTC
BpmI 499 CTGGAG CTGGAG(16/14)
1328 CTGGAG
BsaWI 308 TCCGGA [AT]^CCGG[AT]
728 TCCGGA
BseAI 308 TCCGGA T^CCGGA
728 TCCGGA
BsmAI 583 GTCTC GTCTC(1/5)
949 GTCTC
BsmFI 378 GGGAC GGGAC(10/14)
1278 GGGAC
BspEI 308 TCCGGA T^CCGGA
728 TCCGGA
BstEII 1020 GGTCACC G^GT[ACGT]ACC
1386 GGTGACC
BstPI 1020 GGTCACC G^GT[ACGT]ACC
1386 GGTGACC
Eam1105I 496 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1280 GACAGATCGTC
EarI 312 GAAGAG CTCTTC(1/4)
748 GAAGAG
EcoNI 1251 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1395 CCTCGCCCAGG
EcoO65I 1020 GGTCACC G^GT[ACGT]ACC
1386 GGTGACC
EcoRV 709 GATATC GAT^ATC
1449 GATATC
EcoT14I 1427 CCATGG C^C[AT][AT]GG
1469 CCATGG
HapII 309 CCGG C^CGG
729 CCGG
HpaII 309 CCGG C^CGG
729 CCGG
Ksp632I 312 GAAGAG CTCTTC(1/4)
748 GAAGAG
MroI 308 TCCGGA T^CCGGA
728 TCCGGA
MspI 309 CCGG C^CGG
729 CCGG
NcoI 1427 CCATGG C^CATGG
1469 CCATGG
NlaIV 773 GGACCC GG[ACGT]^[ACGT]CC
1431 GGCCCC
NspI 51 ACATGC [GA]CATG^[CT]
1370 GCATGT
PvuII 188 CAGCTG CAG^CTG
215 CAGCTG
StyI 1427 CCATGG C^C[AT][AT]GG
1469 CCATGG
TaqI 345 TCGA T^CGA
569 TCGA
TthHB8I 345 TCGA T^CGA
569 TCGA
XcmI 1133 CCACCCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1335 CCAAGATGCTAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BbsI BbvI BglI BpuAI BsaJI
BsgI BsiYI BslI BspMI BsrI
BssKI BstNI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoRII
Fnu4HI HaeIII HhaI HinP1I HinfI
HphI ItaI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII PflMI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I