Restriction map for
pFN21AB7337
(Linear) (Six-base) MAPSORT of: KIBB7337.seq Check: 8662 from: 1 to: 3129
KIBB7337.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 28 3129
Size: 28 3101
AccI GT'mk_AC
Cuts at: 0 3103 3129
Size: 3103 26
Acc65I G'GTAC_C
Cuts at: 0 34 1004 3129
Size: 34 970 2125
Fragments arranged by size:
2125 970 34
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1324 2317 2349 2875 3129
Size: 1324 993 32 526 254
Fragments arranged by size:
1324 993 526 254 32
AflII C'TTAA_G
Cuts at: 0 1111 3129
Size: 1111 2018
AflIII A'CryG_T
Cuts at: 0 1713 2514 3129
Size: 1713 801 615
Fragments arranged by size:
1713 801 615
AhdI GACnn_n'nnGTC
Cuts at: 0 132 3129
Size: 132 2997
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2664 3129
Size: 2664 465
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1278 1310 3129
Size: 1278 32 1819
Fragments arranged by size:
1819 1278 32
AlwNI CAG_nnn'CTG
Cuts at: 0 120 2277 2784 3129
Size: 120 2157 507 345
Fragments arranged by size:
2157 507 345 120
ApaI G_GGCC'C
Cuts at: 0 1446 3129
Size: 1446 1683
ApoI r'AATT_y
Cuts at: 0 91 1663 2102 2787 3129
Size: 91 1572 439 685 342
Fragments arranged by size:
1572 685 439 342 91
AvaI C'yCGr_G
Cuts at: 0 3124 3129
Size: 3124 5
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 18 51 3129
Size: 18 33 3078
Fragments arranged by size:
3078 33 18
BamHI G'GATC_C
Cuts at: 0 657 3129
Size: 657 2472
BanI G'GyrC_C
Cuts at: 0 34 1004 3129
Size: 34 970 2125
Fragments arranged by size:
2125 970 34
BanII G_rGCy'C
Cuts at: 0 678 1016 1446 2621 3129
Size: 678 338 430 1175 508
Fragments arranged by size:
1175 678 508 430 338
BbsI GAAGACnn'nnnn_
Cuts at: 0 727 1508 1949 3129
Size: 727 781 441 1180
Fragments arranged by size:
1180 781 727 441
BbvCI CC'TCA_GC
Cuts at: 0 2842 3129
Size: 2842 287
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 859 893 3129
Size: 859 34 2236
Fragments arranged by size:
2236 859 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 172 3054 3129
Size: 172 2882 75
Fragments arranged by size:
2882 172 75
BclI T'GATC_A
Cuts at: 0 1079 3129
Size: 1079 2050
BglII A'GATC_T
Cuts at: 0 360 1107 3129
Size: 360 747 2022
Fragments arranged by size:
2022 747 360
Bme1580I G_kGCm'C
Cuts at: 0 152 1272 1446 3129
Size: 152 1120 174 1683
Fragments arranged by size:
1683 1120 174 152
BmgBI CAC'GTC
Cuts at: 0 1716 2282 3129
Size: 1716 566 847
Fragments arranged by size:
1716 847 566
BmrI ACTGGGnnnn_n'
Cuts at: 0 762 1012 3129
Size: 762 250 2117
Fragments arranged by size:
2117 762 250
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 981 3129
Size: 981 2148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 586 1318 1730 2128 2470 2635 3129
Size: 106 480 732 412 398 342 165 494
Fragments arranged by size:
732 494 480 412 398 342 165 106
Bpu10I CC'TnA_GC
Cuts at: 0 2059 2842 3129
Size: 2059 783 287
Fragments arranged by size:
2059 783 287
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 1188 2419 3129
Size: 198 990 1231 710
Fragments arranged by size:
1231 990 710 198
BsaI GGTCTCn'nnnn_
Cuts at: 0 2239 3129
Size: 2239 890
BsaAI yAC'GTr
Cuts at: 0 603 2517 3129
Size: 603 1914 612
Fragments arranged by size:
1914 612 603
BsaBI GATnn'nnATC
Cuts at: 0 77 1084 3129
Size: 77 1007 2045
Fragments arranged by size:
2045 1007 77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1375 1405 2608 2638 3129
Size: 1375 30 1203 30 491
Fragments arranged by size:
1375 1203 491 30 30
BseYI C'CCAG_C
Cuts at: 0 113 2379 3129
Size: 113 2266 750
Fragments arranged by size:
2266 750 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 733 3129
Size: 346 387 2396
Fragments arranged by size:
2396 387 346
BsiHKAI G_wGCw'C
Cuts at: 0 1016 2621 3129
Size: 1016 1605 508
Fragments arranged by size:
1605 1016 508
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2717 3129
Size: 2717 412
Bsp1286I G_dGCh'C
Cuts at: 0 152 678 1016 1272 1446 2621 3129
Size: 152 526 338 256 174 1175 508
Fragments arranged by size:
1175 526 508 338 256 174 152
BspHI T'CATG_A
Cuts at: 0 1184 3129
Size: 1184 1945
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 28 2783 3129
Size: 28 2755 346
Fragments arranged by size:
2755 346 28
BsrDI GCAATG_nn'
Cuts at: 0 740 2941 3057 3129
Size: 740 2201 116 72
Fragments arranged by size:
2201 740 116 72
BsrGI T'GTAC_A
Cuts at: 0 462 994 1140 3129
Size: 462 532 146 1989
Fragments arranged by size:
1989 532 462 146
BssSI C'ACGA_G
Cuts at: 0 1927 3129
Size: 1927 1202
BstAPI GCAn_nnn'nTGC
Cuts at: 0 744 3129
Size: 744 2385
BstEII G'GTnAC_C
Cuts at: 0 2770 3129
Size: 2770 359
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1436 1836 2557 3129
Size: 1436 400 721 572
Fragments arranged by size:
1436 721 572 400
BstYI r'GATC_y
Cuts at: 0 345 360 414 483 657 1107 3129
Size: 345 15 54 69 174 450 2022
Fragments arranged by size:
2022 450 345 174 69 54 15
BtgI C'CryG_G
Cuts at: 0 1274 3129
Size: 1274 1855
BtsI GCAGTG_nn'
Cuts at: 0 1272 1917 3129
Size: 1272 645 1212
Fragments arranged by size:
1272 1212 645
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1563 1598 3129
Size: 1563 35 1531
Fragments arranged by size:
1563 1531 35
DraI TTT'AAA
Cuts at: 0 1775 3129
Size: 1775 1354
DraIII CAC_nnn'GTG
Cuts at: 0 292 2938 3129
Size: 292 2646 191
Fragments arranged by size:
2646 292 191
DrdI GACnn_nn'nnGTC
Cuts at: 0 2731 3129
Size: 2731 398
EaeI y'GGCC_r
Cuts at: 0 1426 1457 3129
Size: 1426 31 1672
Fragments arranged by size:
1672 1426 31
EarI CTCTTCn'nnn_
Cuts at: 0 884 1856 3004 3129
Size: 884 972 1148 125
Fragments arranged by size:
1148 972 884 125
EcoICRI GAG'CTC
Cuts at: 0 1014 2619 3129
Size: 1014 1605 510
Fragments arranged by size:
1605 1014 510
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 586 1318 1324 1730 2128 2317 2349
Size: 106 480 732 6 406 398 189 32
Cuts at: 2349 2470 2635 2875 3129
Size: 121 165 240 254
Fragments arranged by size:
732 480 406 398 254 240 189 165
121 106 32 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2746 3129
Size: 2746 383
EcoO109I rG'GnC_Cy
Cuts at: 0 1442 1443 1518 2316 2484 3129
Size: 1442 1 75 798 168 645
Fragments arranged by size:
1442 798 645 168 75 1
EcoRI G'AATT_C
Cuts at: 0 91 1663 3129
Size: 91 1572 1466
Fragments arranged by size:
1572 1466 91
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 120 744 2002 2619 3129
Size: 120 624 1258 617 510
Fragments arranged by size:
1258 624 617 510 120
HaeII r_GCGC'y
Cuts at: 0 651 3129
Size: 651 2478
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 118 150 261 293 883 915 970 1002
Size: 118 32 111 32 590 32 55 32
Cuts at: 1002 1321 1353 1950 1982 2252 2284 2603 2635
Size: 319 32 597 32 270 32 319 32
Cuts at: 2635 3129
Size: 494
Fragments arranged by size:
597 590 494 319 319 270 118 111
55 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1983 3104 3129
Size: 1983 1121 25
Fragments arranged by size:
1983 1121 25
HindIII A'AGCT_T
Cuts at: 0 97 2015 2408 3129
Size: 97 1918 393 721
Fragments arranged by size:
1918 721 393 97
KpnI G_GTAC'C
Cuts at: 0 38 1008 3129
Size: 38 970 2121
Fragments arranged by size:
2121 970 38
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1264 1406 2535 3129
Size: 373 891 142 1129 594
Fragments arranged by size:
1129 891 594 373 142
MscI TGG'CCA
Cuts at: 0 1428 1459 3129
Size: 1428 31 1670
Fragments arranged by size:
1670 1428 31
MslI CAynn'nnrTG
Cuts at: 0 1196 3129
Size: 1196 1933
MspA1I CmG'CkG
Cuts at: 0 84 120 806 1424 2477 2634 3129
Size: 84 36 686 618 1053 157 495
Fragments arranged by size:
1053 686 618 495 157 84 36
NcoI C'CATG_G
Cuts at: 0 1274 3129
Size: 1274 1855
NspI r_CATG'y
Cuts at: 0 3110 3129
Size: 3110 19
PasI CC'CwG_GG
Cuts at: 0 144 167 402 471 1829 3129
Size: 144 23 235 69 1358 1300
Fragments arranged by size:
1358 1300 235 144 69 23
PfoI T'CCnGG_A
Cuts at: 0 1616 2128 3129
Size: 1616 512 1001
Fragments arranged by size:
1616 1001 512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2353 2385 3129
Size: 2353 32 744
Fragments arranged by size:
2353 744 32
PpuMI rG'GwC_Cy
Cuts at: 0 2316 2484 3129
Size: 2316 168 645
Fragments arranged by size:
2316 645 168
PshAI GACnn'nnGTC
Cuts at: 0 691 3129
Size: 691 2438
PspOMI G'GGCC_C
Cuts at: 0 1442 3129
Size: 1442 1687
PstI C_TGCA'G
Cuts at: 0 647 1229 1267 1453 2315 2678 2780 3119
Size: 647 582 38 186 862 363 102 339
Cuts at: 3119 3129
Size: 10
Fragments arranged by size:
862 647 582 363 339 186 102 38
10
PvuII CAG'CTG
Cuts at: 0 84 120 806 1424 2477 2634 3129
Size: 84 36 686 618 1053 157 495
Fragments arranged by size:
1053 686 618 495 157 84 36
SacI G_AGCT'C
Cuts at: 0 1016 2621 3129
Size: 1016 1605 508
Fragments arranged by size:
1605 1016 508
SalI G'TCGA_C
Cuts at: 0 3102 3129
Size: 3102 27
SexAI A'CCwGG_T
Cuts at: 0 1093 3129
Size: 1093 2036
SfcI C'TryA_G
Cuts at: 0 643 1225 1263 1449 2311 2674 2776 3115
Size: 643 582 38 186 862 363 102 339
Cuts at: 3115 3129
Size: 14
Fragments arranged by size:
862 643 582 363 339 186 102 38
14
SmlI C'TyrA_G
Cuts at: 0 177 1111 1203 2434 3129
Size: 177 934 92 1231 695
Fragments arranged by size:
1231 934 695 177 92
SspI AAT'ATT
Cuts at: 0 1560 1992 3129
Size: 1560 432 1137
Fragments arranged by size:
1560 1137 432
StuI AGG'CCT
Cuts at: 0 1117 2752 3129
Size: 1117 1635 377
Fragments arranged by size:
1635 1117 377
StyI C'CwwG_G
Cuts at: 0 42 728 1274 2753 3129
Size: 42 686 546 1479 376
Fragments arranged by size:
1479 686 546 376 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1618 3129
Size: 1618 1511
TatI w'GTAC_w
Cuts at: 0 462 994 1140 3129
Size: 462 532 146 1989
Fragments arranged by size:
1989 532 462 146
XbaI T'CTAG_A
Cuts at: 0 363 3129
Size: 363 2766
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 998 3129
Size: 998 2131
XmnI GAAnn'nnTTC
Cuts at: 0 1058 3129
Size: 1058 2071
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AhdI AlfI
AloI AlwNI ApaI ApoI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglII Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaXI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrGI BssSI BstAPI BstEII BstXI BstYI
BtgI BtsI CspCI DraI DraIII DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII KpnI MmeI MscI MslI MspA1I NcoI
NspI PasI PfoI PpiI PpuMI PshAI PspOMI PstI
PvuII SacI SalI SexAI SfcI SmlI SspI StuI
StyI TaqII TatI XbaI XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AgeI AleI ApaLI AscI AseI
AsiSI AvrII BbeI BfrBI BglI BlpI BmtI BsaHI
BsaWI BseRI BsiEI BsiWI BsmI BspEI BsrBI BsrFI
BssHII BstBI BstZ17I Bsu36I BtgZI ClaI EagI EciI
EcoRV FseI FspI FspAI HpaI KasI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PmeI PmlI PsiI PspXI PsrI
PvuI RsrII SacII SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI TstI Tth111I XhoI XmaI ZraI