Restriction map for
pFN21AB7828
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BpmI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI Hin1I
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SpeI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AlwI 292 GGATC GGATC(4/5)
ApaI 943 GGGCCC GGGCC^C
AseI 1488 ATTAAT AT^TAAT
AsnI 1488 ATTAAT AT^TAAT
AspHI 1239 GAGCAC G[AT]GC[AT]^C
AvaII 1174 GGTCC G^G[AT]CC
BbrPI 1246 CACGTG CAC^GTG
BglII 84 AGATCT A^GATCT
BlpI 871 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 871 GCTGAGC GC^T[ACGT]AGC
BseRI 565 GAGGAG GAGGAG(10/8)
BsiHKAI 1239 GAGCAC G[AT]GC[AT]^C
BspMI 903 ACCTGC ACCTGC(4/8)
CelII 871 GCTGAGC GC^T[ACGT]AGC
CfoI 370 GCGC GCG^C
DraIII 1075 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 632 CCATGG C^C[GA][CT]GG
EcoRI 1012 GAATTC G^AATTC
EcoT14I 632 CCATGG C^C[AT][AT]GG
HhaI 370 GCGC GCG^C
HinP1I 370 GCGC G^CGC
HindIII 977 AAGCTT A^AGCTT
NcoI 632 CCATGG C^CATGG
PmaCI 1246 CACGTG CAC^GTG
PmlI 1246 CACGTG CAC^GTG
PshBI 1488 ATTAAT AT^TAAT
PstI 1747 CTGCAG CTGCA^G
SapI 1135 GAAGAGC GCTCTTC(1/4)
SnaBI 1502 TACGTA TAC^GTA
SspI 601 AATATT AAT^ATT
StyI 632 CCATGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BbsI 781 GAAGAC GAAGAC(2/6)
463 GTCTTC
BcnI 25 CCGGG CC^[GC]GG
442 CCCGG
BpuAI 781 GAAGAC GAAGAC(2/6)
463 GTCTTC
BsaBI 79 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1582 GATTTAGATC
BsrDI 468 CATTGC GCAATG(2/0)
1766 GCAATG
BstF5I 273 CATCC GGATG(2/0)
768 CATCC
BstYI 84 AGATCT [GA]^GATC[CT]
292 GGATCT
EarI 232 GAAGAG CTCTTC(1/4)
1135 GAAGAG
FokI 273 CATCC GGATG(9/13)
768 CATCC
HaeIII 944 GGCC GG^CC
1114 GGCC
Ksp632I 232 GAAGAG CTCTTC(1/4)
1135 GAAGAG
MamI 79 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1582 GATTTAGATC
MflI 84 AGATCT [GA]^GATC[CT]
292 GGATCT
MslI 1242 CACTCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1246 CACGTGCATG
NciI 25 CCGGG CC^[GC]GG
442 CCCGG
NlaIV 636 GGTTCC GG[ACGT]^[ACGT]CC
943 GGGCCC
PleI 29 GACTC GAGTC(4/5)
1027 GACTC
PshAI 29 GACTCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
112 GACTTCAGTC
XhoII 84 AGATCT [GA]^GATC[CT]
292 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BanII BbvI BfaI BmyI BsaAI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
HapII HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SphI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I