(Circular) (Six-base) MAPSORT of: pFN21AB7828.seq Check: 5827 from: 1 to: 6520 pFN21AB7828 6520 bp With 182 enzymes: SgfI * April 10, 2009 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2917 2917 Size: 6520 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4330 278 Size: 53 83 186 936 2794 2468 Fragments arranged by size: 2794 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3863 1937 Size: 1926 4594 Acc65I G'GTAC_C Cuts at: 3841 3841 Size: 6520 AclI AA'CG_TT Cuts at: 1563 3819 4647 5020 1563 Size: 2256 828 373 3063 Fragments arranged by size: 3063 2256 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2104 2749 3153 4583 5967 6235 1632 Size: 472 645 404 1430 1384 268 1917 Fragments arranged by size: 1917 1430 1384 645 472 404 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6323 AflIII A'CryG_T Cuts at: 1203 5419 6396 1203 Size: 4216 977 1327 Fragments arranged by size: 4216 1327 977 AgeI A'CCGG_T Cuts at: 5351 5351 Size: 6520 AhdI GACnn_n'nnGTC Cuts at: 5249 5249 Size: 6520 AleI CACnn'nnGTG Cuts at: 1166 3251 1166 Size: 2085 4435 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2915 3269 1184 Size: 1731 354 4435 Fragments arranged by size: 4435 1731 354 AlwNI CAG_nnn'CTG Cuts at: 4263 5835 4263 Size: 1572 4948 ApaI G_GGCC'C Cuts at: 2952 2952 Size: 6520 ApaLI G'TGCA_C Cuts at: 4577 5733 4577 Size: 1156 5364 ApoI r'AATT_y Cuts at: 1493 2249 2818 2855 3017 3829 4055 4207 6316 Size: 756 569 37 162 812 226 152 2109 Cuts at: 6316 1493 Size: 1697 Fragments arranged by size: 2109 1697 812 756 569 226 162 152 37 AseI AT'TA_AT Cuts at: 160 1032 3494 5074 160 Size: 872 2462 1580 1606 Fragments arranged by size: 2462 1606 1580 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6520 AvaI C'yCGr_G Cuts at: 1870 1943 3845 1870 Size: 73 1902 4545 Fragments arranged by size: 4545 1902 73 BamHI G'GATC_C Cuts at: 3850 4217 3850 Size: 367 6153 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3841 5296 618 Size: 325 217 627 2054 1455 1842 Fragments arranged by size: 2054 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2465 2883 2952 3839 1391 Size: 1074 418 69 887 4072 Fragments arranged by size: 4072 1074 887 418 69 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2461 2793 928 Size: 971 562 332 4655 Fragments arranged by size: 4655 971 562 332 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4711 4745 1903 Size: 34 2774 34 3678 Fragments arranged by size: 3678 2774 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4412 5622 4412 Size: 1210 5310 BclI T'GATC_A Cuts at: 1548 1548 Size: 6520 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5131 136 Size: 107 122 71 631 605 3459 1525 Fragments arranged by size: 3459 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2089 6515 2089 Size: 4426 2094 BlpI GC'TnA_GC Cuts at: 2877 3937 2877 Size: 1060 5460 Bme1580I G_kGCm'C Cuts at: 1790 2952 3157 4581 5737 1790 Size: 1162 205 1424 1156 2573 Fragments arranged by size: 2573 1424 1162 1156 205 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6520 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2459 2877 3054 Size: 416 53 83 399 24 1042 418 177 Cuts at: 3054 4251 5209 442 Size: 1197 958 1753 Fragments arranged by size: 1753 1197 1042 958 418 416 399 177 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6520 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5180 1239 Size: 3941 2579 Bpu10I CC'TnA_GC Cuts at: 2514 2514 Size: 6520 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4645 5510 5808 4645 Size: 865 298 5357 Fragments arranged by size: 5357 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5183 882 Size: 617 3684 2219 Fragments arranged by size: 3684 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3253 3509 3710 4311 493 Size: 711 2049 256 201 601 2702 Fragments arranged by size: 2702 2049 711 601 256 201 BsaBI GATnn'nnATC Cuts at: 2088 3591 6514 2088 Size: 1503 2923 2094 Fragments arranged by size: 2923 2094 1503 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4327 4709 5344 275 Size: 53 83 186 936 2794 382 635 1451 Fragments arranged by size: 2794 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4953 5351 5625 5772 6183 4953 Size: 398 274 147 411 5290 Fragments arranged by size: 5290 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6295 Fragments arranged by size: 6295 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2585 1186 Size: 1399 5121 BseYI C'CCAG_C Cuts at: 2043 2271 5723 6219 2043 Size: 228 3452 496 2344 Fragments arranged by size: 3452 2344 496 228 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5947 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3970 4731 5759 1540 Size: 275 186 1969 761 1028 2301 Fragments arranged by size: 2301 1969 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3248 4581 4666 5737 3248 Size: 1333 85 1071 4031 Fragments arranged by size: 4031 1333 1071 85 BsmI GAATG_Cn' Cuts at: 4031 4124 4031 Size: 93 6427 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2465 2883 2952 3157 3248 3839 4581 Size: 399 675 418 69 205 91 591 742 Cuts at: 4581 4666 5737 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 675 591 418 399 205 91 85 69 BspEI T'CCGG_A Cuts at: 6183 6183 Size: 6520 BspHI T'CATG_A Cuts at: 4409 6139 4409 Size: 1730 4790 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2917 3875 844 Size: 2073 958 3489 Fragments arranged by size: 3489 2073 958 BsrBI CCG'CTC Cuts at: 1394 1823 4407 1394 Size: 429 2584 3507 Fragments arranged by size: 3507 2584 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2472 3778 5015 5189 65 Size: 1183 1224 1306 1237 174 1396 Fragments arranged by size: 1396 1306 1237 1224 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5164 5351 1700 Size: 110 3354 187 2869 Fragments arranged by size: 3354 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6520 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6520 BssSI C'ACGA_G Cuts at: 4580 5592 4580 Size: 1012 5508 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6520 BstBI TT'CG_AA Cuts at: 5324 5324 Size: 6520 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6520 BstYI r'GATC_y Cuts at: 1972 2089 2297 3850 4217 4602 4619 6071 6157 Size: 117 208 1553 367 385 17 1452 86 Cuts at: 6157 6169 6515 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1553 1452 385 367 346 208 117 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2637 513 Size: 552 1572 4396 Fragments arranged by size: 4396 1572 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5781 Fragments arranged by size: 5781 683 56 BtsI GCAGTG_nn' Cuts at: 4036 4830 4850 4036 Size: 794 20 5706 Fragments arranged by size: 5706 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6520 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6485 DraI TTT'AAA Cuts at: 3824 3824 Size: 6520 DraIII CAC_nnn'GTG Cuts at: 3085 3085 Size: 6520 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5527 809 Size: 507 4211 1802 Fragments arranged by size: 4211 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3967 4856 6485 Size: 54 1425 50 275 18 2137 889 1629 Cuts at: 6485 8 Size: 43 Fragments arranged by size: 2137 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3967 1537 Size: 275 2155 4090 Fragments arranged by size: 4090 2155 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2232 3135 4450 1353 Size: 879 903 1315 3423 Fragments arranged by size: 3423 1315 903 879 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5107 5481 5627 387 Size: 1064 138 3518 374 146 1280 Fragments arranged by size: 3518 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2104 2749 3153 4583 5180 5967 6235 Size: 393 472 645 404 1430 597 787 268 Cuts at: 6235 1239 Size: 1524 Fragments arranged by size: 1524 1430 787 645 597 472 404 393 268 EcoRI G'AATT_C Cuts at: 3017 3829 4207 3017 Size: 812 378 5330 Fragments arranged by size: 5330 812 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3503 3503 Size: 6520 FspI TGC'GCA Cuts at: 5026 5026 Size: 6520 HaeII r_GCGC'y Cuts at: 1678 5667 1678 Size: 3989 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2564 2596 3017 3049 5161 Size: 32 135 32 992 32 421 32 2112 Cuts at: 5161 5193 5235 5267 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2112 992 421 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2674 2802 2836 3442 3864 4110 Size: 988 281 736 128 34 606 422 246 Cuts at: 4110 669 Size: 3079 Fragments arranged by size: 3079 988 736 606 422 281 246 128 34 HindIII A'AGCT_T Cuts at: 748 2982 748 Size: 2234 4286 HpaI GTT'AAC Cuts at: 4110 4110 Size: 6520 KpnI G_GTAC'C Cuts at: 3845 3845 Size: 6520 MfeI C'AATT_G Cuts at: 4119 4119 Size: 6520 MluI A'CGCG_T Cuts at: 6396 6396 Size: 6520 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5634 5818 1305 Size: 4329 184 2007 Fragments arranged by size: 4329 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4698 Fragments arranged by size: 4698 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3251 3255 4478 4837 4996 518 Size: 648 2085 4 1223 359 159 2042 Fragments arranged by size: 2085 2042 1223 648 359 159 4 MspA1I CmG'CkG Cuts at: 1606 3936 4613 5761 6006 1606 Size: 2330 677 1148 245 2120 Fragments arranged by size: 2330 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2637 513 Size: 552 1572 4396 Fragments arranged by size: 4396 1572 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6520 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6520 NotI GC'GGCC_GC Cuts at: 3967 3967 Size: 6520 NspI r_CATG'y Cuts at: 1132 1229 2365 2902 3706 3878 5423 1132 Size: 97 1136 537 804 172 1545 2229 Fragments arranged by size: 2229 1545 1136 804 537 172 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6520 PciI A'CATG_T Cuts at: 5419 5419 Size: 6520 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6520 PmeI GTTT'AAAC Cuts at: 3824 3824 Size: 6520 PmlI CAC'GTG Cuts at: 3253 3253 Size: 6520 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4589 4621 6102 6134 4589 Size: 32 1481 32 4975 Fragments arranged by size: 4975 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2038 2121 1613 Size: 425 83 6012 Fragments arranged by size: 6012 425 83 PsiI TTA'TAA Cuts at: 4090 4090 Size: 6520 PspOMI G'GGCC_C Cuts at: 2948 2948 Size: 6520 PstI C_TGCA'G Cuts at: 830 3756 3872 830 Size: 2926 116 3478 Fragments arranged by size: 3478 2926 116 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6520 SalI G'TCGA_C Cuts at: 1936 3862 1936 Size: 1926 4594 SapI GCTCTTCn'nnn_ Cuts at: 3135 3135 Size: 6520 SbfI CC_TGCA'GG Cuts at: 3872 3872 Size: 6520 ScaI AGT'ACT Cuts at: 4768 6480 4768 Size: 1712 4808 SfcI C'TryA_G Cuts at: 826 1045 2728 3411 3752 3868 5003 5684 5875 Size: 219 1683 683 341 116 1135 681 191 Cuts at: 5875 826 Size: 1471 Fragments arranged by size: 1683 1471 1135 683 681 341 219 191 116 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6520 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6520 SmaI CCC'GGG Cuts at: 1872 3847 1872 Size: 1975 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4624 5525 5787 820 Size: 197 926 2681 901 262 1553 Fragments arranged by size: 2681 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3509 4311 493 Size: 3016 802 2702 Fragments arranged by size: 3016 2702 802 SpeI A'CTAG_T Cuts at: 152 152 Size: 6520 SphI G_CATG'C Cuts at: 1132 2365 2902 3706 3878 1132 Size: 1233 537 804 172 3774 Fragments arranged by size: 3774 1233 804 537 172 SspI AAT'ATT Cuts at: 5 52 2608 4444 5 Size: 47 2556 1836 2081 Fragments arranged by size: 2556 2081 1836 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6520 StyI C'CwwG_G Cuts at: 513 1065 2637 3959 513 Size: 552 1572 1322 3074 Fragments arranged by size: 3074 1572 1322 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4547 4564 4717 4902 5347 325 Size: 1255 2967 17 153 185 445 1498 Fragments arranged by size: 2967 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2346 3193 4766 Size: 274 80 33 51 1443 369 847 1573 Cuts at: 4766 6478 96 Size: 1712 138 Fragments arranged by size: 1712 1573 1443 847 369 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6480 6512 6480 Size: 32 6488 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6520 XbaI T'CTAG_A Cuts at: 3856 3856 Size: 6520 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6520 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6520 XmaI C'CCGG_G Cuts at: 1870 3845 1870 Size: 1975 4545 XmnI GAAnn'nnTTC Cuts at: 4649 4649 Size: 6520 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4328 276 Size: 53 83 186 936 2794 2468 Fragments arranged by size: 2794 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI