Restriction map for pFN21AB6755
 (Circular) (Six-base) MAPSORT of: pFN21AB6755.seq  Check: 2922  from: 1  to: 5020



pFN21AB6755  5020 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:43  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2830     278 
   Size:         53      83     186     936    1294    2468

  Fragments arranged by size: 

                2468    1294     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2363    1937 
   Size:        426    4594
 
Acc65I G'GTAC_C

Cuts at:   2341    2341 
   Size:       5020
 
AclI AA'CG_TT

Cuts at:   1563    3147    3520    1563 
   Size:       1584     373    3063

  Fragments arranged by size: 

                3063    1584     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2144    3083    4467    4735    1632 
   Size:        512     939    1384     268    1917

  Fragments arranged by size: 

                1917    1384     939     512     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4823
 
AflIII A'CryG_T

Cuts at:   1203    3919    4896    1203 
   Size:       2716     977    1327

  Fragments arranged by size: 

                2716    1327     977
 
AgeI A'CCGG_T

Cuts at:   3851    3851 
   Size:       5020
 
AhdI GACnn_n'nnGTC

Cuts at:   3749    3749 
   Size:       5020
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5020
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5020
 
AlwNI CAG_nnn'CTG

Cuts at:   2763    4335    2763 
   Size:       1572    3448
 
ApaLI G'TGCA_C

Cuts at:   3077    4233    3077 
   Size:       1156    3864
 
ApoI r'AATT_y

Cuts at:   1493    2329    2555    2707    4816    1493 
   Size:        836     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     836     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3574     160 
   Size:        872    2542    1606

  Fragments arranged by size: 

                2542    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5020
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2059    2111    2345    1870 
   Size:         73     116      52     234    4545

  Fragments arranged by size: 

                4545     234     116      73      52
 
BamHI G'GATC_C

Cuts at:   2350    2717    2350 
   Size:        367    4653
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2341    3796     618 
   Size:        325     217     627     554    1455    1842

  Fragments arranged by size: 

                1842    1455     627     554     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2339    1391 
   Size:        948    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4049
 
BbvCI CC'TCA_GC

Cuts at:   2130    2130 
   Size:       5020
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3211    3245    1903 
   Size:         34    1274      34    3678

  Fragments arranged by size: 

                3678    1274      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2912    4122    2912 
   Size:       1210    3810
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5020
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3631     136 
   Size:        107     122      71     631     605    1959    1525

  Fragments arranged by size: 

                1959    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5015    5015 
   Size:       5020
 
BlpI GC'TnA_GC

Cuts at:   2437    2437 
   Size:       5020
 
Bme1580I G_kGCm'C

Cuts at:   1790    3081    4237    1790 
   Size:       1291    1156    2573

  Fragments arranged by size: 

                2573    1291    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5020
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2188    2292    2751 
   Size:        416      53      83     399      24     771     104     459

Cuts at:   2751    3709     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753     958     771     459     416     399     104      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5020
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3680    1239 
   Size:       2441    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2130    2130 
   Size:       5020
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3145    4010    4308    3145 
   Size:        865     298    3857

  Fragments arranged by size: 

                3857     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2114    3683     882 
   Size:        617     615    1569    2219

  Fragments arranged by size: 

                2219    1569     617     615
 
BsaAI yAC'GTr

Cuts at:    493    1204    2811     493 
   Size:        711    1607    2702

  Fragments arranged by size: 

                2702    1607     711
 
BsaBI GATnn'nnATC

Cuts at:   5014    5014 
   Size:       5020
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2017    2827    3209    3844 
   Size:         53      83     186     936     484     810     382     635

Cuts at:   3844     275 
   Size:       1451

  Fragments arranged by size: 

                1451     936     810     635     484     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2259    3453    3851    4125    4272    4683    2259 
   Size:       1194     398     274     147     411    2596

  Fragments arranged by size: 

                2596    1194     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4795

  Fragments arranged by size: 

                4795     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5020
 
BseYI C'CCAG_C

Cuts at:   4223    4719    4223 
   Size:        496    4524
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4447
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2470    3231    4259    1540 
   Size:        275     186     469     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     469     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3081    3166    4237    3081 
   Size:         85    1071    3864

  Fragments arranged by size: 

                3864    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2531    2624    2531 
   Size:         93    4927
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2339    3081    3166    4237    1391 
   Size:        399     549     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     549     399      85
 
BspEI T'CCGG_A

Cuts at:   2259    4683    2259 
   Size:       2424    2596
 
BspHI T'CATG_A

Cuts at:   2909    4639    2909 
   Size:       1730    3290
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2044    2375     844 
   Size:       1200     331    3489

  Fragments arranged by size: 

                3489    1200     331
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2907    1394 
   Size:        429    1084    3507

  Fragments arranged by size: 

                3507    1084     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3515    3689      65 
   Size:       1183    2267     174    1396

  Fragments arranged by size: 

                2267    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3664    3851    1700 
   Size:        110    1854     187    2869

  Fragments arranged by size: 

                2869    1854     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5020
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5020
 
BssSI C'ACGA_G

Cuts at:   3080    4092    3080 
   Size:       1012    4008
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5020
 
BstBI TT'CG_AA

Cuts at:   3824    3824 
   Size:       5020
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5020
 
BstYI r'GATC_y

Cuts at:   1972    2350    2717    3102    3119    4571    4657    4669    5015 
   Size:        378     367     385      17    1452      86      12     346

Cuts at:   5015    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     385     378     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4468
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4281

  Fragments arranged by size: 

                4281     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2536    3330    3350    2536 
   Size:        794      20    4206

  Fragments arranged by size: 

                4206     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5020
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    4985
 
DraI TTT'AAA

Cuts at:   2281    2324    2281 
   Size:         43    4977
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4027     809 
   Size:        507    2711    1802

  Fragments arranged by size: 

                2711    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2467    3356    4985 
   Size:         54    1425      50     275      18     637     889    1629

Cuts at:   4985       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     637     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2467    1537 
   Size:        275     655    4090

  Fragments arranged by size: 

                4090     655     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2950    1353 
   Size:       1597    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3607    3981    4127     387 
   Size:       1064     138    2018     374     146    1280

  Fragments arranged by size: 

                2018    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2144    3083    3680    4467    4735    1239 
   Size:        393     512     939     597     787     268    1524

  Fragments arranged by size: 

                1524     939     787     597     512     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2056    2056 
   Size:       5020
 
EcoRI G'AATT_C

Cuts at:   2329    2707    2329 
   Size:        378    4642
 
FspI TGC'GCA

Cuts at:   3526    3526 
   Size:       5020
 
HaeII r_GCGC'y

Cuts at:   1678    2130    4167    1678 
   Size:        452    2037    2531

  Fragments arranged by size: 

                2531    2037     452
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3661    3693    3735    3767    1373 
   Size:         32     135      32    2089      32      42      32    2626

  Fragments arranged by size: 

                2626    2089     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2364    2610     669 
   Size:        988     281     426     246    3079

  Fragments arranged by size: 

                3079     988     426     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5020
 
HpaI GTT'AAC

Cuts at:   2610    2610 
   Size:       5020
 
KpnI G_GTAC'C

Cuts at:   2345    2345 
   Size:       5020
 
MfeI C'AATT_G

Cuts at:   2619    2619 
   Size:       5020
 
MluI A'CGCG_T

Cuts at:   4896    4896 
   Size:       5020
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4134    4318    1305 
   Size:       2829     184    2007

  Fragments arranged by size: 

                2829    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3198

  Fragments arranged by size: 

                3198    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2978    3337    3496     518 
   Size:        648    1812     359     159    2042

  Fragments arranged by size: 

                2042    1812     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2436    3113    4261    4506    1606 
   Size:        830     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     830     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4468
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5020
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5020
 
NotI GC'GGCC_GC

Cuts at:   2467    2467 
   Size:       5020
 
NspI r_CATG'y

Cuts at:   1132    1229    2378    3923    1132 
   Size:         97    1149    1545    2229

  Fragments arranged by size: 

                2229    1545    1149      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5020
 
PciI A'CATG_T

Cuts at:   3919    3919 
   Size:       5020
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5020
 
PmeI GTTT'AAAC

Cuts at:   2324    2324 
   Size:       5020
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3089    3121    4602    4634    3089 
   Size:         32    1481      32    3475

  Fragments arranged by size: 

                3475    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2056    2056 
   Size:       5020
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5020
 
PsiI TTA'TAA

Cuts at:   2590    2590 
   Size:       5020
 
PstI C_TGCA'G

Cuts at:    830    2140    2372     830 
   Size:       1310     232    3478

  Fragments arranged by size: 

                3478    1310     232
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5020
 
SalI G'TCGA_C

Cuts at:   1936    2362    1936 
   Size:        426    4594
 
SbfI CC_TGCA'GG

Cuts at:   2372    2372 
   Size:       5020
 
ScaI AGT'ACT

Cuts at:   3268    4980    3268 
   Size:       1712    3308
 
SfcI C'TryA_G

Cuts at:    826    1045    2121    2136    2368    3503    4184    4375     826 
   Size:        219    1076      15     232    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1076     681     232     219     191      15
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5020
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5020
 
SmaI CCC'GGG

Cuts at:   1872    2347    1872 
   Size:        475    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3124    4025    4287     820 
   Size:        197     926    1181     901     262    1553

  Fragments arranged by size: 

                1553    1181     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2811     493 
   Size:       2318    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5020
 
SphI G_CATG'C

Cuts at:   1132    2378    1132 
   Size:       1246    3774
 
SspI AAT'ATT

Cuts at:      5      52    2944       5 
   Size:         47    2892    2081

  Fragments arranged by size: 

                2892    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5020
 
StyI C'CwwG_G

Cuts at:    513    1065    2104    2459     513 
   Size:        552    1039     355    3074

  Fragments arranged by size: 

                3074    1039     552     355
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3047    3064    3217    3402    3847     325 
   Size:       1255    1467      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1467    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3266    4978      96 
   Size:        274      80      33      51    1443    1289    1712     138

  Fragments arranged by size: 

                1712    1443    1289     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4980    5012    4980 
   Size:         32    4988
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5020
 
XbaI T'CTAG_A

Cuts at:   2356    2356 
   Size:       5020
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5020
 
XmaI C'CCGG_G

Cuts at:   1870    2345    1870 
   Size:        475    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3149    3149 
   Size:       5020
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2828     276 
   Size:         53      83     186     936    1294    2468

  Fragments arranged by size: 

                2468    1294     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI     SalI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BfrBI     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI   PspOMI
    PspXI     PsrI    PvuII    RsrII     SacI    SacII    SanDI     SapI
    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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