(Circular) (Six-base) MAPSORT of: pFN21AB7807.seq Check: 9580 from: 1 to: 6280 pFN21AB7807 6280 bp With 182 enzymes: SgfI * October 20, 2009 11:01 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2675 2675 Size: 6280 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4090 278 Size: 53 83 186 936 2554 2468 Fragments arranged by size: 2554 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3623 1937 Size: 1686 4594 Acc65I G'GTAC_C Cuts at: 2408 3601 2408 Size: 1193 5087 AclI AA'CG_TT Cuts at: 1563 4407 4780 1563 Size: 2844 373 3063 Fragments arranged by size: 3063 2844 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2326 3126 3149 4343 5727 5995 1632 Size: 694 800 23 1194 1384 268 1917 Fragments arranged by size: 1917 1384 1194 800 694 268 23 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6083 AflIII A'CryG_T Cuts at: 1203 5179 6156 1203 Size: 3976 977 1327 Fragments arranged by size: 3976 1327 977 AgeI A'CCGG_T Cuts at: 5111 5111 Size: 6280 AhdI GACnn_n'nnGTC Cuts at: 5009 5009 Size: 6280 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6280 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6280 AlwNI CAG_nnn'CTG Cuts at: 2218 2357 3194 3379 3444 4023 5595 2218 Size: 139 837 185 65 579 1572 2903 Fragments arranged by size: 2903 1572 837 579 185 139 65 ApaLI G'TGCA_C Cuts at: 4337 5493 4337 Size: 1156 5124 ApoI r'AATT_y Cuts at: 1493 3589 3815 3967 6076 1493 Size: 2096 226 152 2109 1697 Fragments arranged by size: 2109 2096 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4834 160 Size: 872 3802 1606 Fragments arranged by size: 3802 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6280 AvaI C'yCGr_G Cuts at: 1870 1943 2097 3057 3605 1870 Size: 73 154 960 548 4545 Fragments arranged by size: 4545 960 548 154 73 BamHI G'GATC_C Cuts at: 3610 3977 3610 Size: 367 5913 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2295 2408 3601 5056 618 Size: 325 217 627 508 113 1193 1455 1842 Fragments arranged by size: 1842 1455 1193 627 508 325 217 113 BanII G_rGCy'C Cuts at: 1391 2340 2492 2964 3033 3092 3599 1391 Size: 949 152 472 69 59 507 4072 Fragments arranged by size: 4072 949 507 472 152 69 59 BbeI G_GCGC'C Cuts at: 2299 2299 Size: 6280 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5309 BbvCI CC'TCA_GC Cuts at: 3437 3564 3437 Size: 127 6153 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2963 2997 4471 4505 1903 Size: 34 50 34 942 34 1474 34 3678 Fragments arranged by size: 3678 1474 942 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4172 5382 4172 Size: 1210 5070 BclI T'GATC_A Cuts at: 1548 1548 Size: 6280 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4891 136 Size: 107 122 71 631 605 3219 1525 Fragments arranged by size: 3219 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2391 6275 2391 Size: 3884 2396 BlpI GC'TnA_GC Cuts at: 2948 3697 2948 Size: 749 5531 Bme1580I G_kGCm'C Cuts at: 1790 2098 2470 2844 4341 5497 1790 Size: 308 372 374 1497 1156 2573 Fragments arranged by size: 2573 1497 1156 374 372 308 BmgBI CAC'GTC Cuts at: 1318 2209 1318 Size: 891 5389 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2085 2673 2703 Size: 416 53 83 399 24 668 588 30 Cuts at: 2703 2980 3122 4011 4969 442 Size: 277 142 889 958 1753 Fragments arranged by size: 1753 958 889 668 588 416 399 277 142 83 53 30 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6280 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2632 3047 3052 2632 Size: 415 5 5860 Fragments arranged by size: 5860 415 5 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2267 2356 2559 2735 3076 3248 4940 1239 Size: 1028 89 203 176 341 172 1692 2579 Fragments arranged by size: 2579 1692 1028 341 203 176 172 89 Bpu10I CC'TnA_GC Cuts at: 3437 3564 3437 Size: 127 6153 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2069 4405 5270 5568 2069 Size: 2336 865 298 2781 Fragments arranged by size: 2781 2336 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2174 4943 882 Size: 617 675 2769 2219 Fragments arranged by size: 2769 2219 675 617 BsaAI yAC'GTr Cuts at: 493 1204 4071 493 Size: 711 2867 2702 Fragments arranged by size: 2867 2702 711 BsaBI GATnn'nnATC Cuts at: 6274 6274 Size: 6280 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2296 3305 4087 4469 Size: 53 83 186 936 763 1009 782 382 Cuts at: 4469 5104 275 Size: 635 1451 Fragments arranged by size: 1451 1009 936 782 763 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2763 4713 5111 5385 5532 5943 2763 Size: 1950 398 274 147 411 3100 Fragments arranged by size: 3100 1950 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3062 3092 1178 Size: 30 165 30 1659 30 4366 Fragments arranged by size: 4366 1659 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2249 2252 2613 2621 2661 2897 3245 1186 Size: 1063 3 361 8 40 236 348 4221 Fragments arranged by size: 4221 1063 361 348 236 40 8 3 BseYI C'CCAG_C Cuts at: 2122 2669 2786 3221 5483 5979 2122 Size: 547 117 435 2262 496 2423 Fragments arranged by size: 2423 2262 547 496 435 117 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2231 2939 1163 Size: 573 495 708 4504 Fragments arranged by size: 4504 708 573 495 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3424 3730 4491 5519 1540 Size: 275 186 1423 306 761 1028 2301 Fragments arranged by size: 2301 1423 1028 761 306 275 186 BsiHKAI G_wGCw'C Cuts at: 2573 2964 3033 3068 4341 4426 5497 2573 Size: 391 69 35 1273 85 1071 3356 Fragments arranged by size: 3356 1273 1071 391 85 69 35 BsmI GAATG_Cn' Cuts at: 3791 3884 3791 Size: 93 6187 BsmBI CGTCTCn'nnnn_ Cuts at: 2374 3418 2374 Size: 1044 5236 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2098 2340 2470 2492 2573 2844 2964 Size: 399 308 242 130 22 81 271 120 Cuts at: 2964 3033 3068 3092 3599 4341 4426 5497 1391 Size: 69 35 24 507 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 507 399 308 271 242 130 120 85 81 69 35 24 22 BspEI T'CCGG_A Cuts at: 2763 5943 2763 Size: 3180 3100 BspHI T'CATG_A Cuts at: 4169 5899 4169 Size: 1730 4550 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2675 3330 3568 3635 844 Size: 1831 655 238 67 3489 Fragments arranged by size: 3489 1831 655 238 67 BsrBI CCG'CTC Cuts at: 1394 1823 2383 4167 1394 Size: 429 560 1784 3507 Fragments arranged by size: 3507 1784 560 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2437 3332 4775 4949 65 Size: 1183 1189 895 1443 174 1396 Fragments arranged by size: 1443 1396 1189 1183 895 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3573 4924 5111 1700 Size: 110 1763 1351 187 2869 Fragments arranged by size: 2869 1763 1351 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6280 BssHII G'CGCG_C Cuts at: 1925 2155 1925 Size: 230 6050 BssSI C'ACGA_G Cuts at: 2957 4340 5352 2957 Size: 1383 1012 3885 Fragments arranged by size: 3885 1383 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2465 794 Size: 1671 4609 BstBI TT'CG_AA Cuts at: 5084 5084 Size: 6280 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6280 BstYI r'GATC_y Cuts at: 1972 2391 2553 2757 2886 3610 3977 4362 4379 Size: 419 162 204 129 724 367 385 17 Cuts at: 4379 5831 5917 5929 6275 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 724 419 385 367 346 204 162 129 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2655 2655 Size: 6280 BtgI C'CryG_G Cuts at: 513 1065 2103 3343 513 Size: 552 1038 1240 3450 Fragments arranged by size: 3450 1240 1038 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5541 Fragments arranged by size: 5541 683 56 BtsI GCAGTG_nn' Cuts at: 2108 2470 2519 3796 4590 4610 2108 Size: 362 49 1277 794 20 3778 Fragments arranged by size: 3778 1277 794 362 49 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6280 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6245 DraI TTT'AAA Cuts at: 3584 3584 Size: 6280 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5287 809 Size: 507 3971 1802 Fragments arranged by size: 3971 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2271 2558 2911 Size: 54 1425 50 275 18 441 287 353 Cuts at: 2911 3727 4616 6245 8 Size: 816 889 1629 43 Fragments arranged by size: 1629 1425 889 816 441 353 287 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3727 1537 Size: 275 1915 4090 Fragments arranged by size: 4090 1915 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2345 2390 2598 2874 3038 4210 1353 Size: 992 45 208 276 164 1172 3423 Fragments arranged by size: 3423 1172 992 276 208 164 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2061 4867 5241 5387 387 Size: 1064 138 472 2806 374 146 1280 Fragments arranged by size: 2806 1280 1064 472 374 146 138 EcoICRI GAG'CTC Cuts at: 2962 3031 2962 Size: 69 6211 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 2326 2356 2559 2735 3076 3126 Size: 393 635 59 30 203 176 341 50 Cuts at: 3126 3149 3248 4343 4940 5727 5995 1239 Size: 23 99 1095 597 787 268 1524 Fragments arranged by size: 1524 1095 787 635 597 393 341 268 203 176 99 59 50 30 23 EcoNI CCTnn'n_nnAGG Cuts at: 2303 2538 3186 3230 3507 2303 Size: 235 648 44 277 5076 Fragments arranged by size: 5076 648 277 235 44 EcoO109I rG'GnC_Cy Cuts at: 2146 2531 2545 3119 3191 3512 2146 Size: 385 14 574 72 321 4914 Fragments arranged by size: 4914 574 385 321 72 14 EcoRI G'AATT_C Cuts at: 3589 3967 3589 Size: 378 5902 FspI TGC'GCA Cuts at: 4786 4786 Size: 6280 HaeII r_GCGC'y Cuts at: 1678 2018 2061 2299 3243 5427 1678 Size: 340 43 238 944 2184 2531 Fragments arranged by size: 2531 2184 944 340 238 43 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2498 2530 2783 2815 4921 Size: 32 135 32 926 32 253 32 2106 Cuts at: 4921 4953 4995 5027 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2106 926 253 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2348 3624 3870 669 Size: 988 281 410 1276 246 3079 Fragments arranged by size: 3079 1276 988 410 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6280 HpaI GTT'AAC Cuts at: 3870 3870 Size: 6280 KasI G'GCGC_C Cuts at: 2295 2295 Size: 6280 KpnI G_GTAC'C Cuts at: 2412 3605 2412 Size: 1193 5087 MfeI C'AATT_G Cuts at: 3879 3879 Size: 6280 MluI A'CGCG_T Cuts at: 6156 6156 Size: 6280 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2562 3130 5394 5578 1305 Size: 1257 568 2264 184 2007 Fragments arranged by size: 2264 2007 1257 568 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2273 2560 10 Size: 54 1425 343 441 287 3730 Fragments arranged by size: 3730 1425 441 343 287 54 MslI CAynn'nnrTG Cuts at: 518 1166 2738 4238 4597 4756 518 Size: 648 1572 1500 359 159 2042 Fragments arranged by size: 2042 1572 1500 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2137 2218 3696 4373 5521 5766 1606 Size: 531 81 1478 677 1148 245 2120 Fragments arranged by size: 2120 1478 1148 677 531 245 81 NaeI GCC'GGC Cuts at: 3575 3575 Size: 6280 NarI GG'CG_CC Cuts at: 2296 2296 Size: 6280 NcoI C'CATG_G Cuts at: 513 1065 2103 3343 513 Size: 552 1038 1240 3450 Fragments arranged by size: 3450 1240 1038 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6280 NgoMIV G'CCGG_C Cuts at: 3573 3573 Size: 6280 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6280 NotI GC'GGCC_GC Cuts at: 3727 3727 Size: 6280 NspI r_CATG'y Cuts at: 1132 1229 3638 5183 1132 Size: 97 2409 1545 2229 Fragments arranged by size: 2409 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2447 1348 Size: 1099 5181 PciI A'CATG_T Cuts at: 5179 5179 Size: 6280 PflMI CCAn_nnn'nTGG Cuts at: 1108 2525 3344 1108 Size: 1417 819 4044 Fragments arranged by size: 4044 1417 819 PmeI GTTT'AAAC Cuts at: 3584 3584 Size: 6280 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4349 4381 5862 5894 4349 Size: 32 1481 32 4735 Fragments arranged by size: 4735 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2146 2531 2545 3512 2146 Size: 385 14 967 4914 Fragments arranged by size: 4914 967 385 14 PshAI GACnn'nnGTC Cuts at: 1613 3498 1613 Size: 1885 4395 PsiI TTA'TAA Cuts at: 3850 3850 Size: 6280 PstI C_TGCA'G Cuts at: 830 2076 2136 2154 2304 2641 3372 3632 830 Size: 1246 60 18 150 337 731 260 3478 Fragments arranged by size: 3478 1246 731 337 260 150 60 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6280 PvuII CAG'CTG Cuts at: 2137 2218 2137 Size: 81 6199 SacI G_AGCT'C Cuts at: 2964 3033 2964 Size: 69 6211 SalI G'TCGA_C Cuts at: 1936 3622 1936 Size: 1686 4594 SanDI GG'GwC_CC Cuts at: 2545 2545 Size: 6280 SapI GCTCTTCn'nnn_ Cuts at: 2345 2390 3038 2345 Size: 45 648 5587 Fragments arranged by size: 5587 648 45 SbfI CC_TGCA'GG Cuts at: 2641 3632 2641 Size: 991 5289 ScaI AGT'ACT Cuts at: 4528 6240 4528 Size: 1712 4568 SfcI C'TryA_G Cuts at: 826 1045 2072 2132 2150 2300 2637 3368 3628 Size: 219 1027 60 18 150 337 731 260 Cuts at: 3628 4763 5444 5635 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1027 731 681 337 260 219 191 150 60 18 SfoI GGC'GCC Cuts at: 2297 2297 Size: 6280 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6280 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6280 SmaI CCC'GGG Cuts at: 1872 3607 1872 Size: 1735 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2084 4384 5285 5547 820 Size: 197 926 141 2300 901 262 1553 Fragments arranged by size: 2300 1553 926 901 262 197 141 SnaBI TAC'GTA Cuts at: 493 4071 493 Size: 3578 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6280 SphI G_CATG'C Cuts at: 1132 3638 1132 Size: 2506 3774 SspI AAT'ATT Cuts at: 5 52 4204 5 Size: 47 4152 2081 Fragments arranged by size: 4152 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6280 StyI C'CwwG_G Cuts at: 513 1065 2103 2774 3296 3343 3719 513 Size: 552 1038 671 522 47 376 3074 Fragments arranged by size: 3074 1038 671 552 522 376 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2724 4307 4324 4477 4662 5107 325 Size: 1255 1144 1583 17 153 185 445 1498 Fragments arranged by size: 1583 1498 1255 1144 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4526 6238 96 Size: 274 80 33 51 1443 2549 1712 138 Fragments arranged by size: 2549 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2683 2715 6240 6272 2683 Size: 32 3525 32 2691 Fragments arranged by size: 3525 2691 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6280 XbaI T'CTAG_A Cuts at: 3616 3616 Size: 6280 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6280 XmaI C'CCGG_G Cuts at: 1870 3605 1870 Size: 1735 4545 XmnI GAAnn'nnTTC Cuts at: 4409 4409 Size: 6280 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4088 276 Size: 53 83 186 936 2554 2468 Fragments arranged by size: 2554 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI XcmI