Restriction map for
pFN21AE0206
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FokI
FseI FspI HaeII HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MaeI MaeIII MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tsp45I Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 342 CGCG CG^CG
Asp700I 510 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 89 GAGCTC G[AT]GC[AT]^C
BanII 89 GAGCTC G[GA]GC[CT]^C
BbsI 127 GTCTTC GAAGAC(2/6)
BbvCI 260 CCTCAGC CCTCAGC(-5/-2)
BbvI 598 GCAGC GCAGC(8/12)
BclI 141 TGATCA T^GATCA
BcnI 400 CCCGG CC^[GC]GG
BpuAI 127 GTCTTC GAAGAC(2/6)
BsiHKAI 89 GAGCTC G[AT]GC[AT]^C
BsrDI 200 GCAATG GCAATG(2/0)
BsrI 375 ACTGG ACTGG(1/-1)
BstAPI 224 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 342 CGCG CG^CG
BstXI 59 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 152 AGATCC [GA]^GATC[CT]
Cac8I 115 GCCAGC GC[ACGT]^[ACGT]GC
DraI 163 TTTAAA TTT^AAA
DraII 395 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 383 CCATGG C^C[GA][CT]GG
EcoO109I 395 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 383 CCATGG C^C[AT][AT]GG
FbaI 141 TGATCA T^GATCA
HapII 401 CCGG C^CGG
HindIII 272 AAGCTT A^AGCTT
HinfI 171 GAGTC G^A[ACGT]TC
HpaII 401 CCGG C^CGG
MflI 152 AGATCC [GA]^GATC[CT]
MseI 164 TTAA T^TAA
MslI 279 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 401 CCGG C^CGG
MvnI 342 CGCG CG^CG
NciI 400 CCCGG CC^[GC]GG
NcoI 383 CCATGG C^CATGG
PleI 171 GAGTC GAGTC(4/5)
SacI 89 GAGCTC GAGCT^C
SapI 86 GAAGAGC GCTCTTC(1/4)
SfaNI 353 GCATC GCATC(5/9)
SfcI 633 CTACAG C^T[GA][CT]AG
StyI 383 CCATGG C^C[AT][AT]GG
Tru9I 164 TTAA T^TAA
TseI 598 GCAGC G^C[AT]GC
Tsp509I 250 AATT ^AATT
XcmI 291 CCAGAACCGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 152 AGATCC [GA]^GATC[CT]
XmnI 510 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwI 39 GATCC GGATC(4/5)
153 GATCC
BanI 78 GGCACC G^G[CT][GA]CC
109 GGCACC
BmyI 89 GAGCTC G[AGT]GC[ACT]^C
453 GGGCAC
BpmI 193 CTGGAG CTGGAG(16/14)
376 CTGGAG
BsaI 469 GAGACC GGTCTC(1/5)
15 GGTCTC
BsiYI 150 CCAGATCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
431 CCATGTTTGGG
BslI 150 CCAGATCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
431 CCATGTTTGGG
BsmAI 469 GAGAC GTCTC(1/5)
16 GTCTC
BsmFI 329 GGGAC GGGAC(10/14)
147 GTCCC
Bsp1286I 89 GAGCTC G[AGT]GC[ACT]^C
453 GGGCAC
CfoI 198 GCGC GCG^C
343 GCGC
DdeI 7 CTTAG C^T[ACGT]AG
261 CTCAG
EarI 86 GAAGAG CTCTTC(1/4)
667 GAAGAG
HhaI 198 GCGC GCG^C
343 GCGC
HinP1I 198 GCGC G^CGC
343 GCGC
Ksp632I 86 GAAGAG CTCTTC(1/4)
667 GAAGAG
MaeII 125 ACGT A^CGT
650 ACGT
SmlI 32 CTCAAG C^T[CT][GA]AG
324 CTTGAG
TaqI 311 TCGA T^CGA
497 TCGA
TthHB8I 311 TCGA T^CGA
497 TCGA
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI AvaII BsaJI BseRI
BssKI BstNI Cfr13I DpnII EcoRII
Fnu4HI HaeIII HphI ItaI MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
TspRI