(Circular) (Six-base) MAPSORT of: pFN21AB8850.seq Check: 5434 from: 1 to: 4918 pFN21AB8850 4918 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2728 278 Size: 53 83 186 936 1192 2468 Fragments arranged by size: 2468 1192 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2261 1937 Size: 324 4594 Acc65I G'GTAC_C Cuts at: 2239 2239 Size: 4918 AclI AA'CG_TT Cuts at: 1563 3045 3418 1563 Size: 1482 373 3063 Fragments arranged by size: 3063 1482 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2157 2981 4365 4633 1632 Size: 525 824 1384 268 1917 Fragments arranged by size: 1917 1384 824 525 268 AflII C'TTAA_G Cuts at: 820 1017 2060 820 Size: 197 1043 3678 Fragments arranged by size: 3678 1043 197 AflIII A'CryG_T Cuts at: 1203 3817 4794 1203 Size: 2614 977 1327 Fragments arranged by size: 2614 1327 977 AgeI A'CCGG_T Cuts at: 3749 3749 Size: 4918 AhdI GACnn_n'nnGTC Cuts at: 3647 3647 Size: 4918 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4918 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4918 AlwNI CAG_nnn'CTG Cuts at: 2661 4233 2661 Size: 1572 3346 ApaLI G'TGCA_C Cuts at: 2975 4131 2975 Size: 1156 3762 ApoI r'AATT_y Cuts at: 1493 2227 2453 2605 4714 1493 Size: 734 226 152 2109 1697 Fragments arranged by size: 2109 1697 734 226 152 AseI AT'TA_AT Cuts at: 160 1032 3472 160 Size: 872 2440 1606 Fragments arranged by size: 2440 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4918 AvaI C'yCGr_G Cuts at: 1870 1943 2243 1870 Size: 73 300 4545 Fragments arranged by size: 4545 300 73 BamHI G'GATC_C Cuts at: 2248 2615 2248 Size: 367 4551 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2239 3694 618 Size: 325 217 627 452 1455 1842 Fragments arranged by size: 1842 1455 627 452 325 217 BanII G_rGCy'C Cuts at: 1391 2237 1391 Size: 846 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2151 928 Size: 971 252 3695 Fragments arranged by size: 3695 971 252 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3109 3143 1903 Size: 34 1172 34 3678 Fragments arranged by size: 3678 1172 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2810 4020 2810 Size: 1210 3708 BclI T'GATC_A Cuts at: 1548 1548 Size: 4918 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3529 136 Size: 107 122 71 631 605 1857 1525 Fragments arranged by size: 1857 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4913 4913 Size: 4918 BlpI GC'TnA_GC Cuts at: 2335 2335 Size: 4918 Bme1580I G_kGCm'C Cuts at: 1790 2979 4135 1790 Size: 1189 1156 2573 Fragments arranged by size: 2573 1189 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4918 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2649 3607 442 Size: 416 53 83 399 24 1232 958 1753 Fragments arranged by size: 1753 1232 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4918 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3578 1239 Size: 2339 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3043 3908 4206 3043 Size: 865 298 3755 Fragments arranged by size: 3755 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3581 882 Size: 617 2082 2219 Fragments arranged by size: 2219 2082 617 BsaAI yAC'GTr Cuts at: 493 1204 2709 493 Size: 711 1505 2702 Fragments arranged by size: 2702 1505 711 BsaBI GATnn'nnATC Cuts at: 4912 4912 Size: 4918 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2725 3107 3742 275 Size: 53 83 186 936 1192 382 635 1451 Fragments arranged by size: 1451 1192 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3351 3749 4023 4170 4581 3351 Size: 398 274 147 411 3688 Fragments arranged by size: 3688 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2183 2213 1178 Size: 30 165 30 780 30 3883 Fragments arranged by size: 3883 780 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4918 BseYI C'CCAG_C Cuts at: 4121 4617 4121 Size: 496 4422 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4345 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2368 3129 4157 1540 Size: 275 186 367 761 1028 2301 Fragments arranged by size: 2301 1028 761 367 275 186 BsiHKAI G_wGCw'C Cuts at: 2979 3064 4135 2979 Size: 85 1071 3762 Fragments arranged by size: 3762 1071 85 BsmI GAATG_Cn' Cuts at: 2429 2522 2429 Size: 93 4825 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2237 2979 3064 4135 1391 Size: 399 447 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 447 399 85 BspEI T'CCGG_A Cuts at: 4581 4581 Size: 4918 BspHI T'CATG_A Cuts at: 2807 4537 2807 Size: 1730 3188 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2273 844 Size: 1429 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2805 1394 Size: 429 982 3507 Fragments arranged by size: 3507 982 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3413 3587 65 Size: 1183 2165 174 1396 Fragments arranged by size: 2165 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3562 3749 1700 Size: 110 1752 187 2869 Fragments arranged by size: 2869 1752 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4918 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4918 BssSI C'ACGA_G Cuts at: 2978 3990 2978 Size: 1012 3906 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4918 BstBI TT'CG_AA Cuts at: 3722 3722 Size: 4918 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4918 BstXI CCAn_nnnn'nTGG Cuts at: 2047 2047 Size: 4918 BstYI r'GATC_y Cuts at: 1972 2248 2615 3000 3017 4469 4555 4567 4913 Size: 276 367 385 17 1452 86 12 346 Cuts at: 4913 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 276 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3427 Fragments arranged by size: 3427 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4179 Fragments arranged by size: 4179 683 56 BtsI GCAGTG_nn' Cuts at: 2434 3228 3248 2434 Size: 794 20 4104 Fragments arranged by size: 4104 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4918 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4883 DraI TTT'AAA Cuts at: 2222 2222 Size: 4918 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3925 809 Size: 507 2609 1802 Fragments arranged by size: 2609 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2365 3254 Size: 54 1425 50 275 18 177 358 889 Cuts at: 3254 4883 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 358 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2365 1537 Size: 275 553 4090 Fragments arranged by size: 4090 553 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2848 1353 Size: 1495 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3505 3879 4025 387 Size: 1064 138 1916 374 146 1280 Fragments arranged by size: 1916 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2157 2981 3578 4365 4633 1239 Size: 393 525 824 597 787 268 1524 Fragments arranged by size: 1524 824 787 597 525 393 268 EcoRI G'AATT_C Cuts at: 2227 2605 2227 Size: 378 4540 FspI TGC'GCA Cuts at: 3424 3424 Size: 4918 HaeII r_GCGC'y Cuts at: 1678 4065 1678 Size: 2387 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3559 3591 3633 3665 1373 Size: 32 135 32 1987 32 42 32 2626 Fragments arranged by size: 2626 1987 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2262 2508 669 Size: 988 281 324 246 3079 Fragments arranged by size: 3079 988 324 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4918 HpaI GTT'AAC Cuts at: 2508 2508 Size: 4918 KpnI G_GTAC'C Cuts at: 2243 2243 Size: 4918 MfeI C'AATT_G Cuts at: 2517 2517 Size: 4918 MluI A'CGCG_T Cuts at: 4794 4794 Size: 4918 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4032 4216 1305 Size: 2727 184 2007 Fragments arranged by size: 2727 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 2919 Fragments arranged by size: 2919 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2876 3235 3394 518 Size: 648 1710 359 159 2042 Fragments arranged by size: 2042 1710 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2105 2334 3011 4159 4404 1606 Size: 499 229 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 499 245 229 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3427 Fragments arranged by size: 3427 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 4918 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4918 NotI GC'GGCC_GC Cuts at: 2365 2365 Size: 4918 NspI r_CATG'y Cuts at: 1132 1229 2138 2276 3821 1132 Size: 97 909 138 1545 2229 Fragments arranged by size: 2229 1545 909 138 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4918 PciI A'CATG_T Cuts at: 3817 3817 Size: 4918 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4918 PmeI GTTT'AAAC Cuts at: 2222 2222 Size: 4918 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2987 3019 4500 4532 2987 Size: 32 1481 32 3373 Fragments arranged by size: 3373 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4918 PsiI TTA'TAA Cuts at: 2488 2488 Size: 4918 PstI C_TGCA'G Cuts at: 830 2110 2270 830 Size: 1280 160 3478 Fragments arranged by size: 3478 1280 160 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4918 PvuII CAG'CTG Cuts at: 2105 2105 Size: 4918 SalI G'TCGA_C Cuts at: 1936 2260 1936 Size: 324 4594 SbfI CC_TGCA'GG Cuts at: 2270 2270 Size: 4918 ScaI AGT'ACT Cuts at: 3166 4878 3166 Size: 1712 3206 SfcI C'TryA_G Cuts at: 826 1045 2106 2266 3401 4082 4273 826 Size: 219 1061 160 1135 681 191 1471 Fragments arranged by size: 1471 1135 1061 681 219 191 160 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4918 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4918 SmaI CCC'GGG Cuts at: 1872 2245 1872 Size: 373 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2060 3022 3923 4185 820 Size: 197 926 117 962 901 262 1553 Fragments arranged by size: 1553 962 926 901 262 197 117 SnaBI TAC'GTA Cuts at: 493 2709 493 Size: 2216 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4918 SphI G_CATG'C Cuts at: 1132 2276 1132 Size: 1144 3774 SspI AAT'ATT Cuts at: 5 52 2842 5 Size: 47 2790 2081 Fragments arranged by size: 2790 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4918 StyI C'CwwG_G Cuts at: 513 1065 2004 2097 2139 2357 513 Size: 552 939 93 42 218 3074 Fragments arranged by size: 3074 939 552 218 93 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2945 2962 3115 3300 3745 325 Size: 1255 1365 17 153 185 445 1498 Fragments arranged by size: 1498 1365 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3164 4876 96 Size: 274 80 33 51 1443 1187 1712 138 Fragments arranged by size: 1712 1443 1187 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4878 4910 4878 Size: 32 4886 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4918 XbaI T'CTAG_A Cuts at: 2254 2254 Size: 4918 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4918 XmaI C'CCGG_G Cuts at: 1870 2243 1870 Size: 373 4545 XmnI GAAnn'nnTTC Cuts at: 2201 3047 2201 Size: 846 4072 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2726 276 Size: 53 83 186 936 1192 2468 Fragments arranged by size: 2468 1192 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI