(Circular) (Six-base) MAPSORT of: pFN21AB8217.seq Check: 8490 from: 1 to: 5584 pFN21AB8217 5584 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2119 2119 Size: 5584 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3394 278 Size: 53 83 186 936 1858 2468 Fragments arranged by size: 2468 1858 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2853 2927 1937 Size: 916 74 4594 Fragments arranged by size: 4594 916 74 Acc65I G'GTAC_C Cuts at: 2905 2905 Size: 5584 AclI AA'CG_TT Cuts at: 1563 3711 4084 1563 Size: 2148 373 3063 Fragments arranged by size: 3063 2148 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3647 5031 5299 1632 Size: 2015 1384 268 1917 Fragments arranged by size: 2015 1917 1384 268 AfeI AGC'GCT Cuts at: 2168 2168 Size: 5584 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5387 AflIII A'CryG_T Cuts at: 1203 2460 4483 5460 1203 Size: 1257 2023 977 1327 Fragments arranged by size: 2023 1327 1257 977 AgeI A'CCGG_T Cuts at: 4415 4415 Size: 5584 AhdI GACnn_n'nnGTC Cuts at: 2568 4313 2568 Size: 1745 3839 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5584 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5584 AlwNI CAG_nnn'CTG Cuts at: 2662 2838 3327 4899 2662 Size: 176 489 1572 3347 Fragments arranged by size: 3347 1572 489 176 ApaI G_GGCC'C Cuts at: 2026 2198 2803 2026 Size: 172 605 4807 Fragments arranged by size: 4807 605 172 ApaLI G'TGCA_C Cuts at: 3641 4797 3641 Size: 1156 4428 ApoI r'AATT_y Cuts at: 1493 2893 3119 3271 5380 1493 Size: 1400 226 152 2109 1697 Fragments arranged by size: 2109 1697 1400 226 152 AseI AT'TA_AT Cuts at: 160 1032 4138 160 Size: 872 3106 1606 Fragments arranged by size: 3106 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5584 AvaI C'yCGr_G Cuts at: 1870 1943 2286 2769 2909 1870 Size: 73 343 483 140 4545 Fragments arranged by size: 4545 483 343 140 73 BamHI G'GATC_C Cuts at: 2914 3281 2914 Size: 367 5217 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2107 2149 2597 2905 4360 Size: 325 217 627 320 42 448 308 1455 Cuts at: 4360 618 Size: 1842 Fragments arranged by size: 1842 1455 627 448 325 320 308 217 42 BanII G_rGCy'C Cuts at: 1391 2026 2198 2547 2631 2742 2803 2903 1391 Size: 635 172 349 84 111 61 100 4072 Fragments arranged by size: 4072 635 349 172 111 100 84 61 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4613 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3775 3809 1903 Size: 34 50 34 1754 34 3678 Fragments arranged by size: 3678 1754 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3476 4686 3476 Size: 1210 4374 BclI T'GATC_A Cuts at: 1548 1548 Size: 5584 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2229 4195 136 Size: 107 122 71 631 605 557 1966 1525 Fragments arranged by size: 1966 1525 631 605 557 122 107 71 BglII A'GATC_T Cuts at: 5579 5579 Size: 5584 BlpI GC'TnA_GC Cuts at: 2865 3001 2865 Size: 136 5448 Bme1580I G_kGCm'C Cuts at: 1790 2026 2198 2803 3645 4801 1790 Size: 236 172 605 842 1156 2573 Fragments arranged by size: 2573 1156 842 605 236 172 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5584 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2020 2255 2736 Size: 416 53 83 399 24 603 235 481 Cuts at: 2736 3315 4273 442 Size: 579 958 1753 Fragments arranged by size: 1753 958 603 579 481 416 399 235 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5584 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2485 2561 2579 2741 2756 4244 1239 Size: 1246 76 18 162 15 1488 2579 Fragments arranged by size: 2579 1488 1246 162 76 18 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2203 2383 2600 3709 4574 4872 2203 Size: 180 217 1109 865 298 2915 Fragments arranged by size: 2915 1109 865 298 217 180 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2351 2555 4247 882 Size: 617 852 204 1692 2219 Fragments arranged by size: 2219 1692 852 617 204 BsaAI yAC'GTr Cuts at: 493 1204 2461 3375 493 Size: 711 1257 914 2702 Fragments arranged by size: 2702 1257 914 711 BsaBI GATnn'nnATC Cuts at: 5578 5578 Size: 5584 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2778 3391 3773 4408 Size: 53 83 186 936 1245 613 382 635 Cuts at: 4408 275 Size: 1451 Fragments arranged by size: 1451 1245 936 635 613 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2589 4017 4415 4689 4836 5247 2589 Size: 1428 398 274 147 411 2926 Fragments arranged by size: 2926 1428 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5359 Fragments arranged by size: 5359 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5584 BseYI C'CCAG_C Cuts at: 4787 5283 4787 Size: 496 5088 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5011 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3034 3795 4823 1540 Size: 275 186 1033 761 1028 2301 Fragments arranged by size: 2301 1033 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2539 2631 3645 3730 4801 2539 Size: 92 1014 85 1071 3322 Fragments arranged by size: 3322 1071 1014 92 85 BsmI GAATG_Cn' Cuts at: 3095 3188 3095 Size: 93 5491 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2026 2198 2539 2547 2631 2742 2803 Size: 399 236 172 341 8 84 111 61 Cuts at: 2803 2903 3645 3730 4801 1391 Size: 100 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 341 236 172 111 100 85 84 61 8 BspEI T'CCGG_A Cuts at: 2589 5247 2589 Size: 2658 2926 BspHI T'CATG_A Cuts at: 2439 3473 5203 2439 Size: 1034 1730 2820 Fragments arranged by size: 2820 1730 1034 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2119 2939 844 Size: 1275 820 3489 Fragments arranged by size: 3489 1275 820 BsrBI CCG'CTC Cuts at: 1394 1823 3471 1394 Size: 429 1648 3507 Fragments arranged by size: 3507 1648 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4079 4253 65 Size: 1183 2831 174 1396 Fragments arranged by size: 2831 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2269 2409 2749 4228 4415 1700 Size: 110 459 140 340 1479 187 2869 Fragments arranged by size: 2869 1479 459 340 187 140 110 BsrGI T'GTAC_A Cuts at: 96 2463 96 Size: 2367 3217 BssHII G'CGCG_C Cuts at: 1925 2012 1925 Size: 87 5497 BssSI C'ACGA_G Cuts at: 3644 4656 3644 Size: 1012 4572 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5584 BstBI TT'CG_AA Cuts at: 4388 4388 Size: 5584 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5584 BstYI r'GATC_y Cuts at: 1972 2914 3281 3666 3683 5135 5221 5233 5579 Size: 942 367 385 17 1452 86 12 346 Cuts at: 5579 1972 Size: 1977 Fragments arranged by size: 1977 1452 942 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5032 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4845 Fragments arranged by size: 4845 683 56 BtsI GCAGTG_nn' Cuts at: 2641 3100 3894 3914 2641 Size: 459 794 20 4311 Fragments arranged by size: 4311 794 459 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5584 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5549 DraI TTT'AAA Cuts at: 2888 2888 Size: 5584 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4591 809 Size: 507 3275 1802 Fragments arranged by size: 3275 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2407 2664 2782 Size: 54 1425 50 275 18 577 257 118 Cuts at: 2782 3031 3920 5549 8 Size: 249 889 1629 43 Fragments arranged by size: 1629 1425 889 577 275 257 249 118 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3031 1537 Size: 275 1219 4090 Fragments arranged by size: 4090 1219 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3514 1353 Size: 2161 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4171 4545 4691 387 Size: 1064 138 2582 374 146 1280 Fragments arranged by size: 2582 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2629 2629 Size: 5584 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2485 2561 2579 2741 2756 3647 4244 Size: 393 853 76 18 162 15 891 597 Cuts at: 4244 5031 5299 1239 Size: 787 268 1524 Fragments arranged by size: 1524 891 853 787 597 393 268 162 76 18 15 EcoNI CCTnn'n_nnAGG Cuts at: 2699 2699 Size: 5584 EcoO109I rG'GnC_Cy Cuts at: 2022 2305 2451 2659 2692 2022 Size: 283 146 208 33 4914 Fragments arranged by size: 4914 283 208 146 33 EcoRI G'AATT_C Cuts at: 2893 3271 2893 Size: 378 5206 FspI TGC'GCA Cuts at: 4090 4090 Size: 5584 HaeII r_GCGC'y Cuts at: 1678 2170 4731 1678 Size: 492 2561 2531 Fragments arranged by size: 2561 2531 492 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2334 2366 2554 2586 4225 Size: 32 135 32 762 32 188 32 1639 Cuts at: 4225 4257 4299 4331 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1639 762 188 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2854 2928 3174 669 Size: 988 281 916 74 246 3079 Fragments arranged by size: 3079 988 916 281 246 74 HindIII A'AGCT_T Cuts at: 748 748 Size: 5584 HpaI GTT'AAC Cuts at: 3174 3174 Size: 5584 KpnI G_GTAC'C Cuts at: 2909 2909 Size: 5584 MfeI C'AATT_G Cuts at: 3183 3183 Size: 5584 MluI A'CGCG_T Cuts at: 5460 5460 Size: 5584 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2118 2232 4698 4882 1305 Size: 813 114 2466 184 2007 Fragments arranged by size: 2466 2007 813 184 114 MscI TGG'CCA Cuts at: 10 64 1489 1832 2666 10 Size: 54 1425 343 834 2928 Fragments arranged by size: 2928 1425 834 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2438 3542 3901 4060 518 Size: 648 1272 1104 359 159 2042 Fragments arranged by size: 2042 1272 1104 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2019 3000 3677 4825 5070 1606 Size: 413 981 677 1148 245 2120 Fragments arranged by size: 2120 1148 981 677 413 245 NaeI GCC'GGC Cuts at: 2271 2411 2751 2271 Size: 140 340 5104 Fragments arranged by size: 5104 340 140 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5032 NdeI CA'TA_TG Cuts at: 387 387 Size: 5584 NgoMIV G'CCGG_C Cuts at: 2269 2409 2749 2269 Size: 140 340 5104 Fragments arranged by size: 5104 340 140 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5584 NotI GC'GGCC_GC Cuts at: 3031 3031 Size: 5584 NspI r_CATG'y Cuts at: 1132 1229 2437 2482 2942 4487 1132 Size: 97 1208 45 460 1545 2229 Fragments arranged by size: 2229 1545 1208 460 97 45 PasI CC'CwG_GG Cuts at: 1348 2160 2838 1348 Size: 812 678 4094 Fragments arranged by size: 4094 812 678 PciI A'CATG_T Cuts at: 4483 4483 Size: 5584 PflMI CCAn_nnn'nTGG Cuts at: 1108 2662 1108 Size: 1554 4030 PmeI GTTT'AAAC Cuts at: 2888 2888 Size: 5584 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3653 3685 5166 5198 3653 Size: 32 1481 32 4039 Fragments arranged by size: 4039 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2305 2451 2692 2305 Size: 146 241 5197 Fragments arranged by size: 5197 241 146 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5584 PsiI TTA'TAA Cuts at: 3154 3154 Size: 5584 PspOMI G'GGCC_C Cuts at: 2022 2194 2799 2022 Size: 172 605 4807 Fragments arranged by size: 4807 605 172 PstI C_TGCA'G Cuts at: 830 2116 2636 2936 830 Size: 1286 520 300 3478 Fragments arranged by size: 3478 1286 520 300 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5584 SacI G_AGCT'C Cuts at: 2631 2631 Size: 5584 SalI G'TCGA_C Cuts at: 1936 2852 2926 1936 Size: 916 74 4594 Fragments arranged by size: 4594 916 74 SanDI GG'GwC_CC Cuts at: 2451 2451 Size: 5584 SbfI CC_TGCA'GG Cuts at: 2936 2936 Size: 5584 ScaI AGT'ACT Cuts at: 3832 5544 3832 Size: 1712 3872 SfcI C'TryA_G Cuts at: 826 1045 2112 2632 2754 2932 4067 4748 4939 Size: 219 1067 520 122 178 1135 681 191 Cuts at: 4939 826 Size: 1471 Fragments arranged by size: 1471 1135 1067 681 520 219 191 178 122 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5584 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5584 SmaI CCC'GGG Cuts at: 1872 2288 2771 2911 1872 Size: 416 483 140 4545 Fragments arranged by size: 4545 483 416 140 SmlI C'TyrA_G Cuts at: 820 1017 1943 2218 2398 2615 3688 4589 4851 Size: 197 926 275 180 217 1073 901 262 Cuts at: 4851 820 Size: 1553 Fragments arranged by size: 1553 1073 926 901 275 262 217 197 180 SnaBI TAC'GTA Cuts at: 493 3375 493 Size: 2882 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5584 SphI G_CATG'C Cuts at: 1132 2942 1132 Size: 1810 3774 SrfI GCCC'GGGC Cuts at: 2288 2288 Size: 5584 SspI AAT'ATT Cuts at: 5 52 3508 5 Size: 47 3456 2081 Fragments arranged by size: 3456 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5584 StyI C'CwwG_G Cuts at: 513 1065 3023 513 Size: 552 1958 3074 Fragments arranged by size: 3074 1958 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2375 3611 3628 3781 3966 4411 325 Size: 1255 795 1236 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1236 795 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2463 2481 3830 Size: 274 80 33 51 1443 486 18 1349 Cuts at: 3830 5542 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1349 486 274 138 80 51 33 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2075 2107 2117 2149 5544 5576 2075 Size: 32 10 32 3395 32 2083 Fragments arranged by size: 3395 2083 32 32 32 10 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5584 XbaI T'CTAG_A Cuts at: 2920 2920 Size: 5584 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2402 2402 Size: 5584 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5584 XmaI C'CCGG_G Cuts at: 1870 2286 2769 2909 1870 Size: 416 483 140 4545 Fragments arranged by size: 4545 483 416 140 XmnI GAAnn'nnTTC Cuts at: 3713 3713 Size: 5584 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3392 276 Size: 53 83 186 936 1858 2468 Fragments arranged by size: 2468 1858 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SwaI