Restriction map for
pFN21AB8217
(Linear) (Six-base) MAPSORT of: KIBB8217.seq Check: 6323 from: 1 to: 879
KIBB8217.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 114 879
Size: 114 765
AccI GT'mk_AC
Cuts at: 0 848 879
Size: 848 31
AfeI AGC'GCT
Cuts at: 0 163 879
Size: 163 716
AflIII A'CryG_T
Cuts at: 0 455 879
Size: 455 424
AhdI GACnn_n'nnGTC
Cuts at: 0 563 879
Size: 563 316
AlwNI CAG_nnn'CTG
Cuts at: 0 657 833 879
Size: 657 176 46
Fragments arranged by size:
657 176 46
ApaI G_GGCC'C
Cuts at: 0 21 193 798 879
Size: 21 172 605 81
Fragments arranged by size:
605 172 81 21
AvaI C'yCGr_G
Cuts at: 0 281 764 879
Size: 281 483 115
Fragments arranged by size:
483 281 115
BanI G'GyrC_C
Cuts at: 0 102 144 592 879
Size: 102 42 448 287
Fragments arranged by size:
448 287 102 42
BanII G_rGCy'C
Cuts at: 0 21 193 542 626 737 798 879
Size: 21 172 349 84 111 61 81
Fragments arranged by size:
349 172 111 84 81 61 21
BglI GCCn_nnn'nGGC
Cuts at: 0 224 879
Size: 224 655
BlpI GC'TnA_GC
Cuts at: 0 860 879
Size: 860 19
Bme1580I G_kGCm'C
Cuts at: 0 21 193 798 879
Size: 21 172 605 81
Fragments arranged by size:
605 172 81 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 15 250 731 879
Size: 15 235 481 148
Fragments arranged by size:
481 235 148 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 556 574 736 751 879
Size: 480 76 18 162 15 128
Fragments arranged by size:
480 162 128 76 18 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 378 595 879
Size: 198 180 217 284
Fragments arranged by size:
284 217 198 180
BsaI GGTCTCn'nnnn_
Cuts at: 0 346 550 879
Size: 346 204 329
Fragments arranged by size:
346 329 204
BsaAI yAC'GTr
Cuts at: 0 456 879
Size: 456 423
BsaHI Gr'CG_yC
Cuts at: 0 773 879
Size: 773 106
BsaWI w'CCGG_w
Cuts at: 0 584 879
Size: 584 295
BsiHKAI G_wGCw'C
Cuts at: 0 534 626 879
Size: 534 92 253
Fragments arranged by size:
534 253 92
Bsp1286I G_dGCh'C
Cuts at: 0 21 193 534 542 626 737 798 879
Size: 21 172 341 8 84 111 61 81
Fragments arranged by size:
341 172 111 84 81 61 21 8
BspEI T'CCGG_A
Cuts at: 0 584 879
Size: 584 295
BspHI T'CATG_A
Cuts at: 0 434 879
Size: 434 445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 114 879
Size: 114 765
BsrFI r'CCGG_y
Cuts at: 0 264 404 744 879
Size: 264 140 340 135
Fragments arranged by size:
340 264 140 135
BsrGI T'GTAC_A
Cuts at: 0 458 879
Size: 458 421
BssHII G'CGCG_C
Cuts at: 0 7 879
Size: 7 872
BtsI GCAGTG_nn'
Cuts at: 0 636 879
Size: 636 243
EaeI y'GGCC_r
Cuts at: 0 402 659 777 879
Size: 402 257 118 102
Fragments arranged by size:
402 257 118 102
EcoICRI GAG'CTC
Cuts at: 0 624 879
Size: 624 255
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 556 574 736 751 879
Size: 480 76 18 162 15 128
Fragments arranged by size:
480 162 128 76 18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 694 879
Size: 694 185
EcoO109I rG'GnC_Cy
Cuts at: 0 17 300 446 654 687 879
Size: 17 283 146 208 33 192
Fragments arranged by size:
283 208 192 146 33 17
HaeII r_GCGC'y
Cuts at: 0 165 879
Size: 165 714
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 329 361 549 581 879
Size: 329 32 188 32 298
Fragments arranged by size:
329 298 188 32 32
HincII GTy'rAC
Cuts at: 0 849 879
Size: 849 30
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 227 879
Size: 113 114 652
Fragments arranged by size:
652 114 113
MscI TGG'CCA
Cuts at: 0 661 879
Size: 661 218
MslI CAynn'nnrTG
Cuts at: 0 433 879
Size: 433 446
MspA1I CmG'CkG
Cuts at: 0 14 879
Size: 14 865
NaeI GCC'GGC
Cuts at: 0 266 406 746 879
Size: 266 140 340 133
Fragments arranged by size:
340 266 140 133
NgoMIV G'CCGG_C
Cuts at: 0 264 404 744 879
Size: 264 140 340 135
Fragments arranged by size:
340 264 140 135
NspI r_CATG'y
Cuts at: 0 432 477 879
Size: 432 45 402
Fragments arranged by size:
432 402 45
PasI CC'CwG_GG
Cuts at: 0 155 833 879
Size: 155 678 46
Fragments arranged by size:
678 155 46
PflMI CCAn_nnn'nTGG
Cuts at: 0 657 879
Size: 657 222
PpuMI rG'GwC_Cy
Cuts at: 0 300 446 687 879
Size: 300 146 241 192
Fragments arranged by size:
300 241 192 146
PspOMI G'GGCC_C
Cuts at: 0 17 189 794 879
Size: 17 172 605 85
Fragments arranged by size:
605 172 85 17
PstI C_TGCA'G
Cuts at: 0 111 631 879
Size: 111 520 248
Fragments arranged by size:
520 248 111
SacI G_AGCT'C
Cuts at: 0 626 879
Size: 626 253
SalI G'TCGA_C
Cuts at: 0 847 879
Size: 847 32
SanDI GG'GwC_CC
Cuts at: 0 446 879
Size: 446 433
SfcI C'TryA_G
Cuts at: 0 107 627 749 879
Size: 107 520 122 130
Fragments arranged by size:
520 130 122 107
SmaI CCC'GGG
Cuts at: 0 283 766 879
Size: 283 483 113
Fragments arranged by size:
483 283 113
SmlI C'TyrA_G
Cuts at: 0 213 393 610 879
Size: 213 180 217 269
Fragments arranged by size:
269 217 213 180
SrfI GCCC'GGGC
Cuts at: 0 283 879
Size: 283 596
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 370 879
Size: 370 509
TatI w'GTAC_w
Cuts at: 0 458 476 879
Size: 458 18 403
Fragments arranged by size:
458 403 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 70 102 112 144 879
Size: 70 32 10 32 735
Fragments arranged by size:
735 70 32 32 10
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 397 879
Size: 397 482
XmaI C'CCGG_G
Cuts at: 0 281 764 879
Size: 281 483 115
Fragments arranged by size:
483 281 115
Enzymes that do cut:
AarI AccI AfeI AflIII AhdI AlwNI ApaI AvaI
BanI BanII BglI BlpI Bme1580I BmrI BpmI BpuEI
BsaI BsaAI BsaHI BsaWI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrFI BsrGI BssHII BtsI EaeI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII MmeI MscI MslI
MspA1I NaeI NgoMIV NspI PasI PflMI PpuMI PspOMI
PstI SacI SalI SanDI SfcI SmaI SmlI SrfI
TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AgeI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BmgBI BmtI BplI Bpu10I BsaBI BsaXI BseRI
BseYI BsgI BsiEI BsiWI BsmI BsmBI BsrBI BsrDI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI
EarI EciI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacII SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SspI StuI
StyI SwaI Tth111I XbaI XhoI XmnI ZraI