Restriction map for
pFN21AB7952
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
AspI AviII AvrII BalI BbeI
BbrPI BbvCI BciVI BclI BcnI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstF5I BstPI
BstUI BstZ17I Cac8I CfoI Cfr10I
ClaI CpoI DraI DraII DsaI
EaeI EagI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FbaI FokI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI HpaII KasI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 926 GGTACC G^GTACC
AciI 967 GCGG CCGC(-3/-1)
AcsI 821 GAATTC [GA]^AATT[CT]
AfaI 927 GTAC GT^AC
AhdI 440 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1166 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 508 CCTAAGG CC^T[ACGT]AGG
ApoI 821 GAATTC [GA]^AATT[CT]
Asp718I 926 GGTACC G^GTACC
AspEI 440 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 46 CCCGAG C^[CT]CG[GA]G
AvaII 193 GGACC G^G[AT]CC
BamHI 681 GGATCC G^GATCC
BbsI 952 GTCTTC GAAGAC(2/6)
BbvI 1019 GCAGC GCAGC(8/12)
BcgI 166 CGACAGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 472 CTAG C^TAG
BlpI 1171 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1171 GCTGAGC GC^T[ACGT]AGC
BpuAI 952 GTCTTC GAAGAC(2/6)
BsmFI 501 GTCCC GGGAC(10/14)
BsoBI 46 CCCGAG C^[CT]CG[GA]G
BstAPI 1019 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 549 CCATTCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 508 CCTAAGG CC^T[ACGT]AGG
CelII 1171 GCTGAGC GC^T[ACGT]AGC
DraIII 1162 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 492 GACCCTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 440 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 508 CCTAAGG CC^T[ACGT]AGG
EcoRI 821 GAATTC G^AATTC
FauI 967 GCGGG CCCGC(4/6)
Fnu4HI 1019 GCAGC GC^[ACGT]GC
HindIII 1119 AAGCTT A^AGCTT
ItaI 1019 GCAGC GC^[ACGT]GC
KpnI 926 GGTACC GGTAC^C
MaeI 472 CTAG C^TAG
MslI 760 CACAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 169 CAGCTG C[AC]G^C[GT]G
PvuII 169 CAGCTG CAG^CTG
RsaI 927 GTAC GT^AC
SapI 780 GAAGAGC GCTCTTC(1/4)
SfaNI 981 GCATC GCATC(5/9)
SfcI 1038 CTACAG C^T[GA][CT]AG
TseI 1019 GCAGC G^C[AT]GC
Tsp509I 822 AATT ^AATT
XcmI 180 CCAAGAGACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AflIII 249 ACATGT A^C[GA][CT]GT
526 ACATGT
BanI 465 GGTGCC G^G[CT][GA]CC
926 GGTACC
BsaI 184 GAGACC GGTCTC(1/5)
802 GGTCTC
BsgI 1017 GTGCAG GTGCAG(16/14)
1026 GTGCAG
BspLU11I 249 ACATGT A^CATGT
526 ACATGT
Cfr13I 193 GGACC G^G[ACGT]CC
426 GGCCC
EarI 361 GAAGAG CTCTTC(1/4)
780 GAAGAG
EcoT14I 196 CCAAGG C^C[AT][AT]GG
314 CCAAGG
HaeIII 245 GGCC GG^CC
426 GGCC
Ksp632I 361 GAAGAG CTCTTC(1/4)
780 GAAGAG
NspI 249 ACATGT [GA]CATG^[CT]
526 ACATGT
PflMI 349 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1161 CCACACAGTGG
PleI 215 GACTC GAGTC(4/5)
532 GACTC
Sau96I 193 GGACC G^G[ACGT]CC
426 GGCCC
StyI 196 CCAAGG C^C[AT][AT]GG
314 CCAAGG
Tsp45I 92 GTCAC ^GT[GC]AC
530 GTGAC
Van91I 349 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1161 CCACACAGTGG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AluI AlwI Asp700I AspHI BanII
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI BstYI DdeI DpnII EcoRII
HinfI HphI MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV SacI Sau3AI
ScrFI TaqI TfiI Tru9I TspRI
TthHB8I XhoII XmnI