Restriction map for
pFN21AB7952
(Circular) (Six-base) MAPSORT of: pFN21AB7952.seq Check: 9303 from: 1 to: 5887
pFN21AB7952 5887 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3697 278
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3230 1937
Size: 1293 4594
Acc65I G'GTAC_C
Cuts at: 2931 3208 2931
Size: 277 5610
AclI AA'CG_TT
Cuts at: 1563 4014 4387 1563
Size: 2451 373 3063
Fragments arranged by size:
3063 2451 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2486 2860 2944 3950 5334 5602 1632
Size: 854 374 84 1006 1384 268 1917
Fragments arranged by size:
1917 1384 1006 854 374 268 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5690
AflIII A'CryG_T
Cuts at: 1203 2254 2531 4786 5763 1203
Size: 1051 277 2255 977 1327
Fragments arranged by size:
2255 1327 1051 977 277
AgeI A'CCGG_T
Cuts at: 4718 4718
Size: 5887
AhdI GACnn_n'nnGTC
Cuts at: 2450 4616 2450
Size: 2166 3721
AleI CACnn'nnGTG
Cuts at: 1166 2769 1166
Size: 1603 4284
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5887
AlwNI CAG_nnn'CTG
Cuts at: 3176 3630 5202 3176
Size: 454 1572 3861
Fragments arranged by size:
3861 1572 454
ApaLI G'TGCA_C
Cuts at: 3944 5100 3944
Size: 1156 4731
ApoI r'AATT_y
Cuts at: 1493 2826 3196 3422 3574 5683 1493
Size: 1333 370 226 152 2109 1697
Fragments arranged by size:
2109 1697 1333 370 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4441 160
Size: 872 3409 1606
Fragments arranged by size:
3409 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
AvaI C'yCGr_G
Cuts at: 1870 1943 2051 3212 1870
Size: 73 108 1161 4545
Fragments arranged by size:
4545 1161 108 73
BamHI G'GATC_C
Cuts at: 2686 3217 3584 2686
Size: 531 367 4989
Fragments arranged by size:
4989 531 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2470 2931 3208 4663 618
Size: 325 217 627 683 461 277 1455 1842
Fragments arranged by size:
1842 1455 683 627 461 325 277 217
BanII G_rGCy'C
Cuts at: 1391 2328 2406 2804 3206 1391
Size: 937 78 398 402 4072
Fragments arranged by size:
4072 937 402 398 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2950 928
Size: 971 1051 3865
Fragments arranged by size:
3865 1051 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2160 2194 4078 4112 1903
Size: 34 223 34 1884 34 3678
Fragments arranged by size:
3678 1884 223 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3779 4989 3779
Size: 1210 4677
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5887
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4498 136
Size: 107 122 71 631 605 2826 1525
Fragments arranged by size:
2826 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5882 5882
Size: 5887
BlpI GC'TnA_GC
Cuts at: 3177 3304 3177
Size: 127 5760
Bme1580I G_kGCm'C
Cuts at: 1790 3948 5104 1790
Size: 2158 1156 2573
Fragments arranged by size:
2573 2158 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5887
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3618 4576 442
Size: 416 53 83 399 24 2201 958 1753
Fragments arranged by size:
2201 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5887
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2811 2811
Size: 5887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2312 2315 2405 2414 2856 4547 1239
Size: 968 105 3 90 9 442 1691 2579
Fragments arranged by size:
2579 1691 968 442 105 90 9 3
Bpu10I CC'TnA_GC
Cuts at: 2609 2803 2609
Size: 194 5693
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4012 4877 5175 4012
Size: 865 298 4724
Fragments arranged by size:
4724 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2183 2813 4550 882
Size: 617 684 630 1737 2219
Fragments arranged by size:
2219 1737 684 630 617
BsaAI yAC'GTr
Cuts at: 493 1204 3678 493
Size: 711 2474 2702
Fragments arranged by size:
2702 2474 711
BsaBI GATnn'nnATC
Cuts at: 5881 5881
Size: 5887
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3694 4076 4711 275
Size: 53 83 186 936 2161 382 635 1451
Fragments arranged by size:
2161 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4320 4718 4992 5139 5550 4320
Size: 398 274 147 411 4657
Fragments arranged by size:
4657 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5662
Fragments arranged by size:
5662 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5887
BseYI C'CCAG_C
Cuts at: 2334 2508 5090 5586 2334
Size: 174 2582 496 2635
Fragments arranged by size:
2635 2582 496 174
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3043 3052 1163
Size: 573 1307 9 3998
Fragments arranged by size:
3998 1307 573 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3337 4098 5126 1540
Size: 275 186 1336 761 1028 2301
Fragments arranged by size:
2301 1336 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2264 2328 2406 2804 3948 4033 5104 2264
Size: 64 78 398 1144 85 1071 3047
Fragments arranged by size:
3047 1144 1071 398 85 78 64
BsmI GAATG_Cn'
Cuts at: 3398 3491 3398
Size: 93 5794
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2264 2328 2406 2804 3206 3948 4033
Size: 399 474 64 78 398 402 742 85
Cuts at: 4033 5104 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 474 402 399 398 85
78 64
BspEI T'CCGG_A
Cuts at: 5550 5550
Size: 5887
BspHI T'CATG_A
Cuts at: 3776 5506 3776
Size: 1730 4157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3242 844
Size: 2398 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3774 1394
Size: 429 1951 3507
Fragments arranged by size:
3507 1951 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4382 4556 65
Size: 1183 3134 174 1396
Fragments arranged by size:
3134 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4531 4718 1700
Size: 110 2721 187 2869
Fragments arranged by size:
2869 2721 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5887
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5887
BssSI C'ACGA_G
Cuts at: 3947 4959 3947
Size: 1012 4875
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3030 794
Size: 2236 3651
BstBI TT'CG_AA
Cuts at: 4691 4691
Size: 5887
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5887
BstXI CCAn_nnnn'nTGG
Cuts at: 2561 2561
Size: 5887
BstYI r'GATC_y
Cuts at: 1972 2281 2389 2686 3162 3217 3584 3969 3986
Size: 309 108 297 476 55 367 385 17
Cuts at: 3986 5438 5524 5536 5882 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 476 385 367 346 309 297
108 86 55 17 12
Bsu36I CC'TnA_GG
Cuts at: 2514 2514
Size: 5887
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5335
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5148
Fragments arranged by size:
5148 683 56
BtsI GCAGTG_nn'
Cuts at: 2741 2935 3136 3403 4197 4217 2741
Size: 194 201 267 794 20 4411
Fragments arranged by size:
4411 794 267 201 194 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5887
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2064 2099 2929 2964 529
Size: 35 1500 35 830 35 3452
Fragments arranged by size:
3452 1500 830 35 35 35
DraI TTT'AAA
Cuts at: 3191 3191
Size: 5887
DraIII CAC_nnn'GTG
Cuts at: 3172 3172
Size: 5887
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2503 4894 809
Size: 507 1187 2391 1802
Fragments arranged by size:
2391 1802 1187 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3334 4223 5852
Size: 54 1425 50 275 18 1504 889 1629
Cuts at: 5852 8
Size: 43
Fragments arranged by size:
1629 1504 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3334 1537
Size: 275 1522 4090
Fragments arranged by size:
4090 1522 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2361 2780 3817 1353
Size: 1008 419 1037 3423
Fragments arranged by size:
3423 1037 1008 419
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4474 4848 4994 387
Size: 1064 138 2885 374 146 1280
Fragments arranged by size:
2885 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2326 2404 2802 2326
Size: 78 398 5411
Fragments arranged by size:
5411 398 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2312 2315 2405 2414 2486 2856
Size: 393 575 105 3 90 9 72 370
Cuts at: 2856 2860 2944 3950 4547 5334 5602 1239
Size: 4 84 1006 597 787 268 1524
Fragments arranged by size:
1524 1006 787 597 575 393 370 268
105 90 84 72 9 4 3
EcoRI G'AATT_C
Cuts at: 2826 3196 3574 2826
Size: 370 378 5139
Fragments arranged by size:
5139 378 370
FspI TGC'GCA
Cuts at: 4393 4393
Size: 5887
HaeII r_GCGC'y
Cuts at: 1678 5034 1678
Size: 3356 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 2266 2298 2436
Size: 32 135 32 443 32 219 32 138
Cuts at: 2436 2458 2468 2490 2791 2823 2959 2991 3031
Size: 22 10 22 301 32 136 32 40
Cuts at: 3031 3063 4528 4560 4602 4634 1373
Size: 32 1465 32 42 32 2626
Fragments arranged by size:
2626 1465 443 301 219 138 136 135
42 40 32 32 32 32 32 32
32 32 32 22 22 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3231 3477 669
Size: 988 281 1293 246 3079
Fragments arranged by size:
3079 1293 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3124 748
Size: 2376 3511
HpaI GTT'AAC
Cuts at: 3477 3477
Size: 5887
KpnI G_GTAC'C
Cuts at: 2935 3212 2935
Size: 277 5610
MfeI C'AATT_G
Cuts at: 3486 3486
Size: 5887
MluI A'CGCG_T
Cuts at: 5763 5763
Size: 5887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5001 5185 1305
Size: 3696 184 2007
Fragments arranged by size:
3696 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4065
Fragments arranged by size:
4065 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2769 3845 4204 4363 518
Size: 648 1603 1076 359 159 2042
Fragments arranged by size:
2042 1603 1076 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2176 3303 3980 5128 5373 1606
Size: 570 1127 677 1148 245 2120
Fragments arranged by size:
2120 1148 1127 677 570 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5335
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5887
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5887
NotI GC'GGCC_GC
Cuts at: 3334 3334
Size: 5887
NspI r_CATG'y
Cuts at: 1132 1229 2258 2535 3245 4790 1132
Size: 97 1029 277 710 1545 2229
Fragments arranged by size:
2229 1545 1029 710 277 97
PasI CC'CwG_GG
Cuts at: 1348 2035 1348
Size: 687 5200
PciI A'CATG_T
Cuts at: 2254 2531 4786 2254
Size: 277 2255 3355
Fragments arranged by size:
3355 2255 277
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2360 3172 1108
Size: 1252 812 3823
Fragments arranged by size:
3823 1252 812
PfoI T'CCnGG_A
Cuts at: 2223 2223
Size: 5887
PmeI GTTT'AAAC
Cuts at: 3191 3191
Size: 5887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3956 3988 5469 5501 3956
Size: 32 1481 32 4342
Fragments arranged by size:
4342 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5887
PsiI TTA'TAA
Cuts at: 3457 3457
Size: 5887
PstI C_TGCA'G
Cuts at: 830 3239 830
Size: 2409 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5887
PvuII CAG'CTG
Cuts at: 2176 2176
Size: 5887
SacI G_AGCT'C
Cuts at: 2328 2406 2804 2328
Size: 78 398 5411
Fragments arranged by size:
5411 398 78
SalI G'TCGA_C
Cuts at: 1936 3229 1936
Size: 1293 4594
SapI GCTCTTCn'nnn_
Cuts at: 2780 2780
Size: 5887
SbfI CC_TGCA'GG
Cuts at: 3239 3239
Size: 5887
ScaI AGT'ACT
Cuts at: 4135 5847 4135
Size: 1712 4175
SfcI C'TryA_G
Cuts at: 826 1045 3043 3235 4370 5051 5242 826
Size: 219 1998 192 1135 681 191 1471
Fragments arranged by size:
1998 1471 1135 681 219 192 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5887
SmaI CCC'GGG
Cuts at: 1872 3214 1872
Size: 1342 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3991 4892 5154 820
Size: 197 926 2048 901 262 1553
Fragments arranged by size:
2048 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3678 493
Size: 3185 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5887
SphI G_CATG'C
Cuts at: 1132 3245 1132
Size: 2113 3774
SspI AAT'ATT
Cuts at: 5 52 3811 5
Size: 47 3759 2081
Fragments arranged by size:
3759 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5887
StyI C'CwwG_G
Cuts at: 513 1065 2201 2319 3326 513
Size: 552 1136 118 1007 3074
Fragments arranged by size:
3074 1136 1007 552 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2027 2326 3914 3931 4084 4269 4714
Size: 1255 447 299 1588 17 153 185 445
Cuts at: 4714 325
Size: 1498
Fragments arranged by size:
1588 1498 1255 447 445 299 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4133 5845 96
Size: 274 80 33 51 1443 2156 1712 138
Fragments arranged by size:
2156 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5847 5879 5847
Size: 32 5855
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5887
XbaI T'CTAG_A
Cuts at: 3223 3223
Size: 5887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2192 2192
Size: 5887
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5887
XmaI C'CCGG_G
Cuts at: 1870 3212 1870
Size: 1342 4545
XmnI GAAnn'nnTTC
Cuts at: 2067 2873 2957 4016 2067
Size: 806 84 1059 3938
Fragments arranged by size:
3938 1059 806 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3695 276
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstZ17I EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI