Restriction map for
pFN21AB6419
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BlnI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssKI
BssSI Bst1107I BstAPI BstBI BstEII
BstNI BstPI BstUI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRII EcoRV
EcoT14I FauI FbaI FseI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaI HpaII KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MspI MunI MvaI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI ScrFI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AciI 77 GCGG CCGC(-3/-1)
AflIII 774 ACATGT A^C[GA][CT]GT
AlwI 193 GATCC GGATC(4/5)
AlwNI 745 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 259 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 884 GAGCAC G[AT]GC[AT]^C
AvaI 422 CTCGAG C^[CT]CG[GA]G
BanI 705 GGCACC G^G[CT][GA]CC
BbsI 800 GAAGAC GAAGAC(2/6)
BglII 610 AGATCT A^GATCT
BlpI 595 GCTCAGC GC^T[ACGT]AGC
BmyI 884 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 595 GCTCAGC GC^T[ACGT]AGC
BpuAI 800 GAAGAC GAAGAC(2/6)
BsaJI 19 CCTCGG C^C[ACGT][ACGT]GG
BseRI 122 GAGGAG GAGGAG(10/8)
BsiHKAI 884 GAGCAC G[AT]GC[AT]^C
BsmAI 760 GAGAC GTCTC(1/5)
BsmI 898 GCATTC GAATGC(1/-1)
BsoBI 422 CTCGAG C^[CT]CG[GA]G
Bsp1286I 884 GAGCAC G[AGT]GC[ACT]^C
BspLU11I 774 ACATGT A^CATGT
BstF5I 894 GGATG GGATG(2/0)
BstXI 329 CCAACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 540 GCAGGC GC[ACGT]^[ACGT]GC
CelII 595 GCTCAGC GC^T[ACGT]AGC
DraI 813 TTTAAA TTT^AAA
DraII 842 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 842 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 896 ATGCAT ATGCA^T
FokI 894 GGATG GGATG(9/13)
MaeII 664 ACGT A^CGT
MslI 678 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 764 CATATG CA^TATG
NsiI 896 ATGCAT ATGCA^T
NspI 774 ACATGT [GA]CATG^[CT]
PaeR7I 422 CTCGAG C^TCGAG
PleI 420 GACTC GAGTC(4/5)
PpuMI 842 AGGACCT [GA]G^G[AT]CC[CT]
TaqI 423 TCGA T^CGA
TfiI 519 GATTC G^A[AT]TC
TthHB8I 423 TCGA T^CGA
XcmI 353 CCAAAAAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 422 CTCGAG C^TCGAG
XmnI 259 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AfaI 149 GTAC GT^AC
178 GTAC
AvaII 769 GGACC G^G[AT]CC
843 GGACC
BfaI 269 CTAG C^TAG
796 CTAG
BpmI 559 CTGGAG CTGGAG(16/14)
851 CTGGAG
BsiYI 35 CCAAATATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
139 CCTGAGTGTGG
BslI 35 CCAAATATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
139 CCTGAGTGTGG
BstYI 192 AGATCC [GA]^GATC[CT]
610 AGATCT
Cfr13I 769 GGACC G^G[ACGT]CC
843 GGACC
HincII 202 GTTGAC GT[CT]^[GA]AC
689 GTCAAC
HindII 202 GTTGAC GT[CT]^[GA]AC
689 GTCAAC
HindIII 446 AAGCTT A^AGCTT
718 AAGCTT
HinfI 420 GACTC G^A[ACGT]TC
519 GATTC
HphI 66 GGTGA GGTGA(8/7)
835 TCACC
MaeI 269 CTAG C^TAG
796 CTAG
MaeIII 661 GTGAC ^GT[ACGT]AC
878 GTCAC
MflI 192 AGATCC [GA]^GATC[CT]
610 AGATCT
MseI 412 TTAA T^TAA
814 TTAA
MwoI 786 GCTTGAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
909 GCTTTTCTGGC
NlaIII 775 CATG CATG^
820 CATG
NlaIV 44 GGAACC GG[ACGT]^[ACGT]CC
705 GGCACC
PstI 511 CTGCAG CTGCA^G
751 CTGCAG
RsaI 149 GTAC GT^AC
178 GTAC
Sau96I 769 GGACC G^G[ACGT]CC
843 GGACC
Tru9I 412 TTAA T^TAA
814 TTAA
Tsp45I 661 GTGAC ^GT[GC]AC
878 GTCAC
XhoII 192 AGATCC [GA]^GATC[CT]
610 AGATCT
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI BbvI BsrI DdeI DpnII
Fnu4HI ItaI MboI MboII MnlI
NdeII Sau3AI SfaNI SfcI SmlI
TseI Tsp509I TspRI