(Circular) (Six-base) MAPSORT of: pFN21AB7905.seq Check: 1681 from: 1 to: 5077 pFN21AB7905 5077 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2887 278 Size: 53 83 186 936 1351 2468 Fragments arranged by size: 2468 1351 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2420 1937 Size: 483 4594 Acc65I G'GTAC_C Cuts at: 2398 2398 Size: 5077 AclI AA'CG_TT Cuts at: 1563 3204 3577 1563 Size: 1641 373 3063 Fragments arranged by size: 3063 1641 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3140 4524 4792 1632 Size: 1508 1384 268 1917 Fragments arranged by size: 1917 1508 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4880 AflIII A'CryG_T Cuts at: 1203 3976 4953 1203 Size: 2773 977 1327 Fragments arranged by size: 2773 1327 977 AgeI A'CCGG_T Cuts at: 3908 3908 Size: 5077 AhdI GACnn_n'nnGTC Cuts at: 3806 3806 Size: 5077 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5077 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5077 AlwNI CAG_nnn'CTG Cuts at: 2820 4392 2820 Size: 1572 3505 ApaLI G'TGCA_C Cuts at: 3134 4290 3134 Size: 1156 3921 ApoI r'AATT_y Cuts at: 1493 2386 2612 2764 4873 1493 Size: 893 226 152 2109 1697 Fragments arranged by size: 2109 1697 893 226 152 AseI AT'TA_AT Cuts at: 160 1032 3631 160 Size: 872 2599 1606 Fragments arranged by size: 2599 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5077 AvaI C'yCGr_G Cuts at: 1870 1943 2402 1870 Size: 73 459 4545 Fragments arranged by size: 4545 459 73 BamHI G'GATC_C Cuts at: 2407 2774 2407 Size: 367 4710 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2398 3853 618 Size: 325 217 627 611 1455 1842 Fragments arranged by size: 1842 1455 627 611 325 217 BanII G_rGCy'C Cuts at: 1391 2396 1391 Size: 1005 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2028 2239 2257 928 Size: 971 129 211 18 3748 Fragments arranged by size: 3748 971 211 129 18 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3268 3302 1903 Size: 34 1331 34 3678 Fragments arranged by size: 3678 1331 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2969 4179 2969 Size: 1210 3867 BclI T'GATC_A Cuts at: 1548 1548 Size: 5077 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3688 136 Size: 107 122 71 631 605 2016 1525 Fragments arranged by size: 2016 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2179 5072 2179 Size: 2893 2184 BlpI GC'TnA_GC Cuts at: 2494 2494 Size: 5077 Bme1580I G_kGCm'C Cuts at: 1790 3138 4294 1790 Size: 1348 1156 2573 Fragments arranged by size: 2573 1348 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5077 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2808 3766 442 Size: 416 53 83 399 24 1391 958 1753 Fragments arranged by size: 1753 1391 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5077 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3737 1239 Size: 2498 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3202 4067 4365 3202 Size: 865 298 3914 Fragments arranged by size: 3914 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3740 882 Size: 617 2241 2219 Fragments arranged by size: 2241 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2868 493 Size: 711 1664 2702 Fragments arranged by size: 2702 1664 711 BsaBI GATnn'nnATC Cuts at: 5071 5071 Size: 5077 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2884 3266 3901 275 Size: 53 83 186 936 1351 382 635 1451 Fragments arranged by size: 1451 1351 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3510 3908 4182 4329 4740 3510 Size: 398 274 147 411 3847 Fragments arranged by size: 3847 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4852 Fragments arranged by size: 4852 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2073 1186 Size: 887 4190 BseYI C'CCAG_C Cuts at: 4280 4776 4280 Size: 496 4581 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2085 1163 Size: 573 349 4155 Fragments arranged by size: 4155 573 349 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2527 3288 4316 1540 Size: 275 186 526 761 1028 2301 Fragments arranged by size: 2301 1028 761 526 275 186 BsiHKAI G_wGCw'C Cuts at: 3138 3223 4294 3138 Size: 85 1071 3921 Fragments arranged by size: 3921 1071 85 BsmI GAATG_Cn' Cuts at: 2588 2681 2588 Size: 93 4984 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2396 3138 3223 4294 1391 Size: 399 606 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 606 399 85 BspEI T'CCGG_A Cuts at: 4740 4740 Size: 5077 BspHI T'CATG_A Cuts at: 2966 4696 2966 Size: 1730 3347 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2432 844 Size: 1588 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2964 1394 Size: 429 1141 3507 Fragments arranged by size: 3507 1141 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2226 3572 3746 65 Size: 1183 978 1346 174 1396 Fragments arranged by size: 1396 1346 1183 978 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3721 3908 1700 Size: 110 1911 187 2869 Fragments arranged by size: 2869 1911 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5077 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5077 BssSI C'ACGA_G Cuts at: 3137 4149 3137 Size: 1012 4065 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5077 BstBI TT'CG_AA Cuts at: 2275 3881 2275 Size: 1606 3471 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5077 BstYI r'GATC_y Cuts at: 1972 2179 2372 2407 2774 3159 3176 4628 4714 Size: 207 193 35 367 385 17 1452 86 Cuts at: 4714 4726 5072 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 207 193 86 35 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3586 Fragments arranged by size: 3586 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2128 487 Size: 683 56 902 3436 Fragments arranged by size: 3436 902 683 56 BtsI GCAGTG_nn' Cuts at: 2593 3387 3407 2593 Size: 794 20 4263 Fragments arranged by size: 4263 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5077 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5042 DraI TTT'AAA Cuts at: 2381 2381 Size: 5077 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4084 809 Size: 507 2768 1802 Fragments arranged by size: 2768 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2524 3413 5042 Size: 54 1425 50 275 18 694 889 1629 Cuts at: 5042 8 Size: 43 Fragments arranged by size: 1629 1425 889 694 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2524 1537 Size: 275 712 4090 Fragments arranged by size: 4090 712 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3007 1353 Size: 1654 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3664 4038 4184 387 Size: 1064 138 2075 374 146 1280 Fragments arranged by size: 2075 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3140 3737 4524 4792 1239 Size: 393 1508 597 787 268 1524 Fragments arranged by size: 1524 1508 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2281 2281 Size: 5077 EcoRI G'AATT_C Cuts at: 2386 2764 2386 Size: 378 4699 EcoRV GAT'ATC Cuts at: 2260 2260 Size: 5077 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2330 2330 Size: 5077 FspI TGC'GCA Cuts at: 3583 3583 Size: 5077 HaeII r_GCGC'y Cuts at: 1678 4224 1678 Size: 2546 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3718 3750 3792 3824 1373 Size: 32 135 32 2146 32 42 32 2626 Fragments arranged by size: 2626 2146 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2421 2667 669 Size: 988 281 483 246 3079 Fragments arranged by size: 3079 988 483 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5077 HpaI GTT'AAC Cuts at: 2667 2667 Size: 5077 KpnI G_GTAC'C Cuts at: 2402 2402 Size: 5077 MfeI C'AATT_G Cuts at: 2226 2676 2226 Size: 450 4627 MluI A'CGCG_T Cuts at: 4953 4953 Size: 5077 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4191 4375 1305 Size: 2886 184 2007 Fragments arranged by size: 2886 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3255 Fragments arranged by size: 3255 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2204 3035 3394 3553 518 Size: 648 1038 831 359 159 2042 Fragments arranged by size: 2042 1038 831 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2493 3170 4318 4563 1606 Size: 887 677 1148 245 2120 Fragments arranged by size: 2120 1148 887 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3586 Fragments arranged by size: 3586 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5077 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5077 NotI GC'GGCC_GC Cuts at: 2524 2524 Size: 5077 NspI r_CATG'y Cuts at: 1132 1229 2435 3980 1132 Size: 97 1206 1545 2229 Fragments arranged by size: 2229 1545 1206 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5077 PciI A'CATG_T Cuts at: 3976 3976 Size: 5077 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5077 PmeI GTTT'AAAC Cuts at: 2381 2381 Size: 5077 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3146 3178 4659 4691 3146 Size: 32 1481 32 3532 Fragments arranged by size: 3532 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2281 2281 Size: 5077 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5077 PsiI TTA'TAA Cuts at: 2647 2647 Size: 5077 PstI C_TGCA'G Cuts at: 830 2429 830 Size: 1599 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5077 SalI G'TCGA_C Cuts at: 1936 2419 1936 Size: 483 4594 SbfI CC_TGCA'GG Cuts at: 2429 2429 Size: 5077 ScaI AGT'ACT Cuts at: 3325 5037 3325 Size: 1712 3365 SfcI C'TryA_G Cuts at: 826 1045 2425 3560 4241 4432 826 Size: 219 1380 1135 681 191 1471 Fragments arranged by size: 1471 1380 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5077 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5077 SmaI CCC'GGG Cuts at: 1872 2404 1872 Size: 532 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3181 4082 4344 820 Size: 197 926 1238 901 262 1553 Fragments arranged by size: 1553 1238 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2868 493 Size: 2375 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5077 SphI G_CATG'C Cuts at: 1132 2435 1132 Size: 1303 3774 SspI AAT'ATT Cuts at: 5 52 3001 5 Size: 47 2949 2081 Fragments arranged by size: 2949 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5077 StyI C'CwwG_G Cuts at: 513 1065 2004 2285 2516 513 Size: 552 939 281 231 3074 Fragments arranged by size: 3074 939 552 281 231 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3104 3121 3274 3459 3904 325 Size: 1255 1524 17 153 185 445 1498 Fragments arranged by size: 1524 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2245 3323 5035 Size: 274 80 33 51 1443 268 1078 1712 Cuts at: 5035 96 Size: 138 Fragments arranged by size: 1712 1443 1078 274 268 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5037 5069 5037 Size: 32 5045 Tth111I GACn'n_nGTC Cuts at: 1316 2238 1316 Size: 922 4155 XbaI T'CTAG_A Cuts at: 2413 2413 Size: 5077 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5077 XmaI C'CCGG_G Cuts at: 1870 2402 1870 Size: 532 4545 XmnI GAAnn'nnTTC Cuts at: 3206 3206 Size: 5077 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2885 276 Size: 53 83 186 936 1351 2468 Fragments arranged by size: 2468 1351 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI