(Circular) (Six-base) MAPSORT of: pFN21AB5387.seq Check: 2625 from: 1 to: 6334 pFN21AB5387 6334 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4144 278 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3677 1937 Size: 1740 4594 Acc65I G'GTAC_C Cuts at: 3655 3655 Size: 6334 AclI AA'CG_TT Cuts at: 1563 4461 4834 1563 Size: 2898 373 3063 Fragments arranged by size: 3063 2898 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4397 5781 6049 1632 Size: 2765 1384 268 1917 Fragments arranged by size: 2765 1917 1384 268 AfeI AGC'GCT Cuts at: 3524 3524 Size: 6334 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6137 AflIII A'CryG_T Cuts at: 1203 5233 6210 1203 Size: 4030 977 1327 Fragments arranged by size: 4030 1327 977 AgeI A'CCGG_T Cuts at: 5165 5165 Size: 6334 AhdI GACnn_n'nnGTC Cuts at: 5063 5063 Size: 6334 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6334 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3621 1184 Size: 2437 3897 AlwNI CAG_nnn'CTG Cuts at: 2647 4077 5649 2647 Size: 1430 1572 3332 Fragments arranged by size: 3332 1572 1430 ApaI G_GGCC'C Cuts at: 2776 2776 Size: 6334 ApaLI G'TGCA_C Cuts at: 3095 4391 5547 3095 Size: 1296 1156 3882 Fragments arranged by size: 3882 1296 1156 ApoI r'AATT_y Cuts at: 1493 2168 3643 3869 4021 6130 1493 Size: 675 1475 226 152 2109 1697 Fragments arranged by size: 2109 1697 1475 675 226 152 AseI AT'TA_AT Cuts at: 160 1032 4888 160 Size: 872 3856 1606 Fragments arranged by size: 3856 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 AvaI C'yCGr_G Cuts at: 1870 1943 2574 3659 1870 Size: 73 631 1085 4545 Fragments arranged by size: 4545 1085 631 73 BamHI G'GATC_C Cuts at: 2620 3664 4031 2620 Size: 1044 367 4923 Fragments arranged by size: 4923 1044 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2206 2668 3193 3655 5110 Size: 325 217 627 419 462 525 462 1455 Cuts at: 5110 618 Size: 1842 Fragments arranged by size: 1842 1455 627 525 462 462 419 325 217 BanII G_rGCy'C Cuts at: 1391 2156 2433 2702 2776 3588 3653 1391 Size: 765 277 269 74 812 65 4072 Fragments arranged by size: 4072 812 765 277 269 74 65 BbeI G_GCGC'C Cuts at: 2672 2672 Size: 6334 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5363 BbvCI CC'TCA_GC Cuts at: 2447 2829 2447 Size: 382 5952 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4525 4559 1903 Size: 34 2588 34 3678 Fragments arranged by size: 3678 2588 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4226 5436 4226 Size: 1210 5124 BclI T'GATC_A Cuts at: 1548 2085 2200 1548 Size: 537 115 5682 Fragments arranged by size: 5682 537 115 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4945 136 Size: 107 122 71 631 605 3273 1525 Fragments arranged by size: 3273 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6329 6329 Size: 6334 BlpI GC'TnA_GC Cuts at: 3751 3751 Size: 6334 Bme1580I G_kGCm'C Cuts at: 1790 2096 2776 3099 4395 5551 1790 Size: 306 680 323 1296 1156 2573 Fragments arranged by size: 2573 1296 1156 680 323 306 BmgBI CAC'GTC Cuts at: 1318 2287 3329 1318 Size: 969 1042 4323 Fragments arranged by size: 4323 1042 969 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2334 3118 3547 Size: 416 53 83 399 24 917 784 429 Cuts at: 3547 4065 5023 442 Size: 518 958 1753 Fragments arranged by size: 1753 958 917 784 518 429 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6334 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2140 2298 2445 2999 4994 1239 Size: 901 158 147 554 1995 2579 Fragments arranged by size: 2579 1995 901 554 158 147 Bpu10I CC'TnA_GC Cuts at: 2122 2447 2829 2122 Size: 325 382 5627 Fragments arranged by size: 5627 382 325 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4459 5324 5622 4459 Size: 865 298 5171 Fragments arranged by size: 5171 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2039 2274 3436 3487 4997 882 Size: 617 540 235 1162 51 1510 2219 Fragments arranged by size: 2219 1510 1162 617 540 235 51 BsaAI yAC'GTr Cuts at: 493 1204 3232 4125 493 Size: 711 2028 893 2702 Fragments arranged by size: 2702 2028 893 711 BsaBI GATnn'nnATC Cuts at: 6328 6328 Size: 6334 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2669 4141 4523 5158 Size: 53 83 186 936 1136 1472 382 635 Cuts at: 5158 275 Size: 1451 Fragments arranged by size: 1472 1451 1136 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3501 4767 5165 5439 5586 5997 3501 Size: 1266 398 274 147 411 3838 Fragments arranged by size: 3838 1266 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2271 2301 2526 2556 1178 Size: 30 165 30 868 30 225 30 4956 Fragments arranged by size: 4956 868 225 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2456 2477 2562 2692 3017 3596 1186 Size: 1270 21 85 130 325 579 3924 Fragments arranged by size: 3924 1270 579 325 130 85 21 BseYI C'CCAG_C Cuts at: 2271 3078 5537 6033 2271 Size: 807 2459 496 2572 Fragments arranged by size: 2572 2459 807 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2988 1163 Size: 573 1252 4509 Fragments arranged by size: 4509 1252 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2839 3055 3784 4545 5573 1540 Size: 275 186 838 216 729 761 1028 2301 Fragments arranged by size: 2301 1028 838 761 729 275 216 186 BsiHKAI G_wGCw'C Cuts at: 2433 3099 3588 4395 4480 5551 2433 Size: 666 489 807 85 1071 3216 Fragments arranged by size: 3216 1071 807 666 489 85 BsmI GAATG_Cn' Cuts at: 2895 3845 3938 2895 Size: 950 93 5291 Fragments arranged by size: 5291 950 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2096 2156 2433 2702 2776 3099 3588 Size: 399 306 60 277 269 74 323 489 Cuts at: 3588 3653 4395 4480 5551 1391 Size: 65 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 489 399 323 306 277 269 85 74 65 60 BspEI T'CCGG_A Cuts at: 5997 5997 Size: 6334 BspHI T'CATG_A Cuts at: 3150 4223 5953 3150 Size: 1073 1730 3531 Fragments arranged by size: 3531 1730 1073 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3007 3589 3689 844 Size: 2163 582 100 3489 Fragments arranged by size: 3489 2163 582 100 BsrBI CCG'CTC Cuts at: 1394 1823 2121 4221 1394 Size: 429 298 2100 3507 Fragments arranged by size: 3507 2100 429 298 BsrDI GCAATG_nn' Cuts at: 65 1248 2880 4829 5003 65 Size: 1183 1632 1949 174 1396 Fragments arranged by size: 1949 1632 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2891 3045 3108 4978 5165 1700 Size: 110 1081 154 63 1870 187 2869 Fragments arranged by size: 2869 1870 1081 187 154 110 63 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6334 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6334 BssSI C'ACGA_G Cuts at: 2852 3534 4394 5406 2852 Size: 682 860 1012 3780 Fragments arranged by size: 3780 1012 860 682 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6334 BstBI TT'CG_AA Cuts at: 5138 5138 Size: 6334 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6334 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3322 2011 Size: 1311 5023 BstYI r'GATC_y Cuts at: 1972 2620 3664 4031 4416 4433 5885 5971 5983 Size: 648 1044 367 385 17 1452 86 12 Cuts at: 5983 6329 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1044 648 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2704 2704 Size: 6334 BtgI C'CryG_G Cuts at: 513 1065 2004 3189 513 Size: 552 939 1185 3658 Fragments arranged by size: 3658 1185 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5595 Fragments arranged by size: 5595 683 56 BtsI GCAGTG_nn' Cuts at: 2534 3005 3315 3850 4644 4664 2534 Size: 471 310 535 794 20 4204 Fragments arranged by size: 4204 794 535 471 310 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6334 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6299 DraI TTT'AAA Cuts at: 3638 3638 Size: 6334 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5341 809 Size: 507 4025 1802 Fragments arranged by size: 4025 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2631 3781 Size: 54 1425 50 275 18 183 618 1150 Cuts at: 3781 4670 6299 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1150 889 618 275 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3781 1537 Size: 275 1969 4090 Fragments arranged by size: 4090 1969 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3411 4264 1353 Size: 2058 853 3423 Fragments arranged by size: 3423 2058 853 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4921 5295 5441 387 Size: 1064 138 3332 374 146 1280 Fragments arranged by size: 3332 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2431 3586 2431 Size: 1155 5179 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2140 2298 2445 2999 4397 4994 5781 Size: 393 508 158 147 554 1398 597 787 Cuts at: 5781 6049 1239 Size: 268 1524 Fragments arranged by size: 1524 1398 787 597 554 508 393 268 158 147 EcoO109I rG'GnC_Cy Cuts at: 2772 2773 2772 Size: 1 6333 EcoRI G'AATT_C Cuts at: 3643 4021 3643 Size: 378 5956 FspI TGC'GCA Cuts at: 2321 4840 2321 Size: 2519 3815 HaeII r_GCGC'y Cuts at: 1678 2672 2959 3526 5481 1678 Size: 994 287 567 1955 2531 Fragments arranged by size: 2531 1955 994 567 287 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2050 2082 2271 2303 2399 Size: 32 135 32 478 32 189 32 96 Cuts at: 2399 2431 2605 2637 4975 5007 5049 5081 1373 Size: 32 174 32 2338 32 42 32 2626 Fragments arranged by size: 2626 2338 478 189 174 135 96 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3678 3924 669 Size: 988 281 1740 246 3079 Fragments arranged by size: 3079 1740 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6334 HpaI GTT'AAC Cuts at: 3924 3924 Size: 6334 KasI G'GCGC_C Cuts at: 2668 2668 Size: 6334 KpnI G_GTAC'C Cuts at: 3659 3659 Size: 6334 MfeI C'AATT_G Cuts at: 3933 3933 Size: 6334 MluI A'CGCG_T Cuts at: 6210 6210 Size: 6334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2356 5448 5632 1305 Size: 1051 3092 184 2007 Fragments arranged by size: 3092 2007 1051 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2015 2633 10 Size: 54 1425 343 183 618 3711 Fragments arranged by size: 3711 1425 618 343 183 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2075 2520 3167 4292 4651 4810 Size: 648 843 66 445 647 1125 359 159 Cuts at: 4810 518 Size: 2042 Fragments arranged by size: 2042 1125 843 648 647 445 359 159 66 MspA1I CmG'CkG Cuts at: 1606 2726 2919 3750 4427 5575 5820 1606 Size: 1120 193 831 677 1148 245 2120 Fragments arranged by size: 2120 1148 1120 831 677 245 193 NaeI GCC'GGC Cuts at: 2893 3110 2893 Size: 217 6117 NarI GG'CG_CC Cuts at: 2669 2669 Size: 6334 NcoI C'CATG_G Cuts at: 513 1065 2004 3189 513 Size: 552 939 1185 3658 Fragments arranged by size: 3658 1185 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6334 NgoMIV G'CCGG_C Cuts at: 2891 3108 2891 Size: 217 6117 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6334 NotI GC'GGCC_GC Cuts at: 3781 3781 Size: 6334 NspI r_CATG'y Cuts at: 1132 1229 3692 5237 1132 Size: 97 2463 1545 2229 Fragments arranged by size: 2463 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6334 PciI A'CATG_T Cuts at: 5233 5233 Size: 6334 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6334 PfoI T'CCnGG_A Cuts at: 2172 3611 2172 Size: 1439 4895 PmeI GTTT'AAAC Cuts at: 3638 3638 Size: 6334 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4403 4435 5916 5948 4403 Size: 32 1481 32 4789 Fragments arranged by size: 4789 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6334 PsiI TTA'TAA Cuts at: 3904 3904 Size: 6334 PspOMI G'GGCC_C Cuts at: 2772 2772 Size: 6334 PspXI vC'TCGA_Gb Cuts at: 2574 2574 Size: 6334 PstI C_TGCA'G Cuts at: 830 2145 3686 830 Size: 1315 1541 3478 Fragments arranged by size: 3478 1541 1315 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6334 PvuII CAG'CTG Cuts at: 2726 2726 Size: 6334 SacI G_AGCT'C Cuts at: 2433 3588 2433 Size: 1155 5179 SalI G'TCGA_C Cuts at: 1936 3676 1936 Size: 1740 4594 SbfI CC_TGCA'GG Cuts at: 3686 3686 Size: 6334 ScaI AGT'ACT Cuts at: 4582 6294 4582 Size: 1712 4622 SfcI C'TryA_G Cuts at: 826 1045 2141 3682 4817 5498 5689 826 Size: 219 1096 1541 1135 681 191 1471 Fragments arranged by size: 1541 1471 1135 1096 681 219 191 SfoI GGC'GCC Cuts at: 2670 2670 Size: 6334 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6334 SmaI CCC'GGG Cuts at: 1872 3661 1872 Size: 1789 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2574 4438 5339 5601 820 Size: 197 926 631 1864 901 262 1553 Fragments arranged by size: 1864 1553 926 901 631 262 197 SnaBI TAC'GTA Cuts at: 493 4125 493 Size: 3632 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6334 SphI G_CATG'C Cuts at: 1132 3692 1132 Size: 2560 3774 SspI AAT'ATT Cuts at: 5 52 4258 5 Size: 47 4206 2081 Fragments arranged by size: 4206 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6334 StyI C'CwwG_G Cuts at: 513 1065 2004 2886 3189 3773 513 Size: 552 939 882 303 584 3074 Fragments arranged by size: 3074 939 882 584 552 303 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3069 4361 4378 4531 4716 5161 325 Size: 1255 1489 1292 17 153 185 445 1498 Fragments arranged by size: 1498 1489 1292 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2682 4580 6292 Size: 274 80 33 51 1443 705 1898 1712 Cuts at: 6292 96 Size: 138 Fragments arranged by size: 1898 1712 1443 705 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2526 2558 6294 6326 2526 Size: 32 3736 32 2534 Fragments arranged by size: 3736 2534 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2285 1316 Size: 969 5365 XbaI T'CTAG_A Cuts at: 3670 3670 Size: 6334 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3382 3382 Size: 6334 XhoI C'TCGA_G Cuts at: 1943 2574 1943 Size: 631 5703 XmaI C'CCGG_G Cuts at: 1870 3659 1870 Size: 1789 4545 XmnI GAAnn'nnTTC Cuts at: 4463 4463 Size: 6334 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4142 276 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI