Restriction map for
pFN21AE0413
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AvaII
AviII BbeI BbrPI BbvCI BciVI
BclI BglI BglII BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BspDI BspEI BspMI
BsrBI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CfoI Cfr13I ClaI CpoI
DraI DraII DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HphI KasI KpnI
KspI MaeII MamI MluI MroI
MslI MvnI NarI NcoI NdeI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI Sau96I SbfI ScaI SexAI
SfcI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AciI 9 GCGG CCGC(-3/-1)
AflII 825 CTTAAG C^TTAAG
AflIII 666 ACATGT A^C[GA][CT]GT
AhdI 986 GACAAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 986 GACAAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 40 CCCGGG C^[CT]CG[GA]G
AvrII 852 CCTAGG C^CTAGG
BamHI 720 GGATCC G^GATCC
BanI 103 GGTGCC G^G[CT][GA]CC
BanII 19 GAGCTC G[GA]GC[CT]^C
BcgI 576 CGAAGAAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 825 CTTAAG C^TTAAG
BlnI 852 CCTAGG C^CTAGG
BlpI 877 GCTCAGC GC^T[ACGT]AGC
BpmI 511 CTGGAG CTGGAG(16/14)
Bpu1102I 877 GCTCAGC GC^T[ACGT]AGC
BsaI 901 GAGACC GGTCTC(1/5)
BseRI 61 GAGGAG GAGGAG(10/8)
BsmI 713 GAATGC GAATGC(1/-1)
BsoBI 40 CCCGGG C^[CT]CG[GA]G
Bsp1407I 129 TGTACA T^GTACA
BspHI 246 TCATGA T^CATGA
BspLU11I 666 ACATGT A^CATGT
BsrDI 755 GCAATG GCAATG(2/0)
BsrFI 692 GCCGGC [GA]^CCGG[CT]
BsrGI 129 TGTACA T^GTACA
BstBI 932 TTCGAA TT^CGAA
BstNI 510 CCTGG CC^[AT]GG
BstYI 720 GGATCC [GA]^GATC[CT]
CelII 877 GCTCAGC GC^T[ACGT]AGC
Cfr10I 692 GCCGGC [GA]^CCGG[CT]
DraIII 684 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 841 GACAGCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 986 GACAAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 847 CCTGTCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 510 CCTGG ^CC[AT]GG
FauI 9 GCGGG CCCGC(4/6)
HincII 70 GTTAAC GT[CT]^[GA]AC
HindII 70 GTTAAC GT[CT]^[GA]AC
HpaI 70 GTTAAC GTT^AAC
MfeI 791 CAATTG C^AATTG
MflI 720 GGATCC [GA]^GATC[CT]
MspA1I 371 CAGCTG C[AC]G^C[GT]G
MunI 791 CAATTG C^AATTG
MvaI 510 CCTGG CC^[AT]GG
NaeI 692 GCCGGC GCC^GGC
NgoMIV 692 GCCGGC G^CCGGC
NheI 699 GCTAGC G^CTAGC
NspV 932 TTCGAA TT^CGAA
PvuII 371 CAGCTG CAG^CTG
RcaI 246 TCATGA T^CATGA
SacI 19 GAGCTC GAGCT^C
SapI 182 GAAGAGC GCTCTTC(1/4)
SfuI 932 TTCGAA TT^CGAA
SmaI 40 CCCGGG CCC^GGG
SmlI 825 CTTAAG C^T[CT][GA]AG
SphI 159 GCATGC GCATG^C
SspBI 129 TGTACA T^GTACA
XhoII 720 GGATCC [GA]^GATC[CT]
XmaCI 40 CCCGGG C^CCGGG
XmaI 40 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 130 GTAC GT^AC
271 GTAC
AspHI 19 GAGCTC G[AT]GC[AT]^C
857 GAGCAC
BalI 680 TGGCCA TGG^CCA
915 TGGCCA
BbsI 290 GAAGAC GAAGAC(2/6)
448 GAAGAC
BcnI 40 CCCGG CC^[GC]GG
41 CCGGG
BmyI 19 GAGCTC G[AGT]GC[ACT]^C
857 GAGCAC
BpuAI 290 GAAGAC GAAGAC(2/6)
448 GAAGAC
BsiHKAI 19 GAGCTC G[AT]GC[AT]^C
857 GAGCAC
BsiYI 740 CCTTTCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
847 CCTGTCCTAGG
BslI 740 CCTTTCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
847 CCTGTCCTAGG
BsmAI 901 GAGAC GTCTC(1/5)
984 GAGAC
Bsp1286I 19 GAGCTC G[AGT]GC[ACT]^C
857 GAGCAC
BstF5I 75 CATCC GGATG(2/0)
342 GGATG
DpnII 640 GATC ^GATC
721 GATC
EaeI 680 TGGCCA [CT]^GGCC[GA]
915 TGGCCA
EarI 182 GAAGAG CTCTTC(1/4)
708 GAAGAG
EcoT14I 852 CCTAGG C^C[AT][AT]GG
905 CCTTGG
FokI 75 CATCC GGATG(9/13)
342 GGATG
HapII 41 CCGG C^CGG
693 CCGG
HpaII 41 CCGG C^CGG
693 CCGG
Ksp632I 182 GAAGAG CTCTTC(1/4)
708 GAAGAG
MboI 640 GATC ^GATC
721 GATC
MluNI 680 TGGCCA TGG^CCA
915 TGGCCA
MscI 680 TGGCCA TGG^CCA
915 TGGCCA
MspI 41 CCGG C^CGG
693 CCGG
MwoI 361 GCAAAGGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
376 GCTGTTGGAGC
NciI 40 CCCGG CC^[GC]GG
41 CCGGG
NdeII 640 GATC ^GATC
721 GATC
RsaI 130 GTAC GT^AC
271 GTAC
Sau3AI 640 GATC ^GATC
721 GATC
StyI 852 CCTAGG C^C[AT][AT]GG
905 CCTTGG
TaqI 575 TCGA T^CGA
933 TCGA
Tsp45I 589 GTGAC ^GT[GC]AC
821 GTCAC
TthHB8I 575 TCGA T^CGA
933 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AcsI AluI AlwI AlwNI ApoI
BbvI BfaI BsaJI BsgI BsrI
BssKI Cac8I DdeI Fnu4HI HaeIII
HinfI ItaI MaeI MaeIII MboII
MnlI MseI NlaIII NlaIV NspI
ScrFI SfaNI TfiI Tru9I TseI
Tsp509I TspRI