(Circular) (Six-base) MAPSORT of: pFN21AE0413.seq Check: 6826 from: 1 to: 5707 pFN21AE0413 5707 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3517 278 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3050 1937 Size: 1113 4594 Acc65I G'GTAC_C Cuts at: 3028 3028 Size: 5707 AclI AA'CG_TT Cuts at: 1563 3834 4207 1563 Size: 2271 373 3063 Fragments arranged by size: 3063 2271 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3770 5154 5422 1632 Size: 2138 1384 268 1917 Fragments arranged by size: 2138 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2830 820 Size: 197 1813 3697 Fragments arranged by size: 3697 1813 197 AflIII A'CryG_T Cuts at: 1203 2671 4606 5583 1203 Size: 1468 1935 977 1327 Fragments arranged by size: 1935 1468 1327 977 AgeI A'CCGG_T Cuts at: 4538 4538 Size: 5707 AhdI GACnn_n'nnGTC Cuts at: 2996 4436 2996 Size: 1440 4267 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5707 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5707 AlwNI CAG_nnn'CTG Cuts at: 2327 2381 2597 3450 5022 2327 Size: 54 216 853 1572 3012 Fragments arranged by size: 3012 1572 853 216 54 ApaLI G'TGCA_C Cuts at: 3764 4920 3764 Size: 1156 4551 ApoI r'AATT_y Cuts at: 1493 2144 2201 2359 2473 3016 3242 3394 5503 Size: 651 57 158 114 543 226 152 2109 Cuts at: 5503 1493 Size: 1697 Fragments arranged by size: 2109 1697 651 543 226 158 152 114 57 AseI AT'TA_AT Cuts at: 160 1032 4261 160 Size: 872 3229 1606 Fragments arranged by size: 3229 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 AvaI C'yCGr_G Cuts at: 1870 1943 2045 3032 1870 Size: 73 102 987 4545 Fragments arranged by size: 4545 987 102 73 AvrII C'CTAG_G Cuts at: 2857 2857 Size: 5707 BamHI G'GATC_C Cuts at: 2725 3037 3404 2725 Size: 312 367 5028 Fragments arranged by size: 5028 367 312 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2108 3028 4483 618 Size: 325 217 627 321 920 1455 1842 Fragments arranged by size: 1842 1455 920 627 325 321 217 BanII G_rGCy'C Cuts at: 1391 2028 3026 1391 Size: 637 998 4072 Fragments arranged by size: 4072 998 637 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2302 2460 928 Size: 971 403 158 4175 Fragments arranged by size: 4175 971 403 158 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2570 2604 3898 3932 1903 Size: 34 633 34 1294 34 3678 Fragments arranged by size: 3678 1294 633 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3599 4809 3599 Size: 1210 4497 BclI T'GATC_A Cuts at: 1548 1548 Size: 5707 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4318 136 Size: 107 122 71 631 605 2646 1525 Fragments arranged by size: 2646 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5702 5702 Size: 5707 BlpI GC'TnA_GC Cuts at: 2883 3124 2883 Size: 241 5466 Bme1580I G_kGCm'C Cuts at: 1790 3768 4924 1790 Size: 1978 1156 2573 Fragments arranged by size: 2573 1978 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5707 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2124 3438 4396 Size: 416 53 83 399 24 707 1314 958 Cuts at: 4396 442 Size: 1753 Fragments arranged by size: 1753 1314 958 707 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2708 1056 Size: 1652 4055 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2411 2898 2411 Size: 487 5220 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2537 4367 1239 Size: 1298 1830 2579 Fragments arranged by size: 2579 1830 1298 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3832 4697 4995 3832 Size: 865 298 4544 Fragments arranged by size: 4544 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2900 4370 882 Size: 617 1401 1470 2219 Fragments arranged by size: 2219 1470 1401 617 BsaAI yAC'GTr Cuts at: 493 1204 3498 493 Size: 711 2294 2702 Fragments arranged by size: 2702 2294 711 BsaBI GATnn'nnATC Cuts at: 5701 5701 Size: 5707 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3514 3896 4531 275 Size: 53 83 186 936 1981 382 635 1451 Fragments arranged by size: 1981 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4140 4538 4812 4959 5370 4140 Size: 398 274 147 411 4477 Fragments arranged by size: 4477 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2523 2553 1178 Size: 30 165 30 1120 30 4332 Fragments arranged by size: 4332 1120 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2081 1186 Size: 895 4812 BseYI C'CCAG_C Cuts at: 2819 4910 5406 2819 Size: 2091 496 3120 Fragments arranged by size: 3120 2091 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2304 2610 2636 1163 Size: 573 568 306 26 4234 Fragments arranged by size: 4234 573 568 306 26 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3157 3918 4946 1540 Size: 275 186 1156 761 1028 2301 Fragments arranged by size: 2301 1156 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2028 2866 3768 3853 4924 2028 Size: 838 902 85 1071 2811 Fragments arranged by size: 2811 1071 902 838 85 BsmI GAATG_Cn' Cuts at: 2724 3218 3311 2724 Size: 494 93 5120 Fragments arranged by size: 5120 494 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2866 3026 3768 3853 4924 1391 Size: 399 238 838 160 742 85 1071 2174 Fragments arranged by size: 2174 1071 838 742 399 238 160 85 BspEI T'CCGG_A Cuts at: 5370 5370 Size: 5707 BspHI T'CATG_A Cuts at: 2251 3596 5326 2251 Size: 1345 1730 2632 Fragments arranged by size: 2632 1730 1345 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3062 844 Size: 2218 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3594 1394 Size: 429 1771 3507 Fragments arranged by size: 3507 1771 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2767 4202 4376 65 Size: 1183 1519 1435 174 1396 Fragments arranged by size: 1519 1435 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2697 4351 4538 1700 Size: 110 887 1654 187 2869 Fragments arranged by size: 2869 1654 887 187 110 BsrGI T'GTAC_A Cuts at: 96 2134 96 Size: 2038 3669 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5707 BssSI C'ACGA_G Cuts at: 3767 4779 3767 Size: 1012 4695 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5707 BstBI TT'CG_AA Cuts at: 2938 4511 2938 Size: 1573 4134 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5707 BstYI r'GATC_y Cuts at: 1972 2725 3037 3404 3789 3806 5258 5344 5356 Size: 753 312 367 385 17 1452 86 12 Cuts at: 5356 5702 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 753 385 367 346 312 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4216 Fragments arranged by size: 4216 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4968 Fragments arranged by size: 4968 683 56 BtsI GCAGTG_nn' Cuts at: 2598 3223 4017 4037 2598 Size: 625 794 20 4268 Fragments arranged by size: 4268 794 625 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5707 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5672 DraI TTT'AAA Cuts at: 3011 3011 Size: 5707 DraIII CAC_nnn'GTG Cuts at: 2694 2694 Size: 5707 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2852 4714 809 Size: 507 1536 1862 1802 Fragments arranged by size: 1862 1802 1536 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2685 2920 3154 Size: 54 1425 50 275 18 855 235 234 Cuts at: 3154 4043 5672 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 855 275 235 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3154 1537 Size: 275 1342 4090 Fragments arranged by size: 4090 1342 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2182 2708 3637 1353 Size: 829 526 929 3423 Fragments arranged by size: 3423 929 829 526 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4294 4668 4814 387 Size: 1064 138 2705 374 146 1280 Fragments arranged by size: 2705 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2026 2026 Size: 5707 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2537 3770 4367 5154 5422 1239 Size: 393 905 1233 597 787 268 1524 Fragments arranged by size: 1524 1233 905 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2856 2856 Size: 5707 EcoRI G'AATT_C Cuts at: 3016 3394 3016 Size: 378 5329 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2761 2975 2761 Size: 214 5493 FspI TGC'GCA Cuts at: 4213 4213 Size: 5707 HaeII r_GCGC'y Cuts at: 1678 4854 1678 Size: 3176 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 2982 3014 4348 Size: 32 135 32 743 32 635 32 1334 Cuts at: 4348 4380 4422 4454 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1334 743 635 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2077 3051 3297 669 Size: 988 281 139 974 246 3079 Fragments arranged by size: 3079 988 974 281 246 139 HindIII A'AGCT_T Cuts at: 748 748 Size: 5707 HpaI GTT'AAC Cuts at: 2077 3297 2077 Size: 1220 4487 KpnI G_GTAC'C Cuts at: 3032 3032 Size: 5707 MfeI C'AATT_G Cuts at: 2796 3306 2796 Size: 510 5197 MluI A'CGCG_T Cuts at: 5583 5583 Size: 5707 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2202 2365 4821 5005 1305 Size: 897 163 2456 184 2007 Fragments arranged by size: 2456 2007 897 184 163 MscI TGG'CCA Cuts at: 10 64 1489 1832 2687 2922 10 Size: 54 1425 343 855 235 2795 Fragments arranged by size: 2795 1425 855 343 235 54 MslI CAynn'nnrTG Cuts at: 518 1166 3665 4024 4183 518 Size: 648 2499 359 159 2042 Fragments arranged by size: 2499 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2378 3123 3800 4948 5193 1606 Size: 772 745 677 1148 245 2120 Fragments arranged by size: 2120 1148 772 745 677 245 NaeI GCC'GGC Cuts at: 2699 2699 Size: 5707 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4216 Fragments arranged by size: 4216 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5707 NgoMIV G'CCGG_C Cuts at: 2697 2697 Size: 5707 NheI G'CTAG_C Cuts at: 1052 2704 1052 Size: 1652 4055 NotI GC'GGCC_GC Cuts at: 3154 3154 Size: 5707 NspI r_CATG'y Cuts at: 1132 1229 2168 2675 2793 3065 4610 1132 Size: 97 939 507 118 272 1545 2229 Fragments arranged by size: 2229 1545 939 507 272 118 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5707 PciI A'CATG_T Cuts at: 2671 4606 2671 Size: 1935 3772 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5707 PfoI T'CCnGG_A Cuts at: 2514 2514 Size: 5707 PmeI GTTT'AAAC Cuts at: 3011 3011 Size: 5707 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3776 3808 5289 5321 3776 Size: 32 1481 32 4162 Fragments arranged by size: 4162 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5707 PsiI TTA'TAA Cuts at: 3277 3277 Size: 5707 PstI C_TGCA'G Cuts at: 830 3059 830 Size: 2229 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5707 PvuII CAG'CTG Cuts at: 2378 2378 Size: 5707 SacI G_AGCT'C Cuts at: 2028 2028 Size: 5707 SalI G'TCGA_C Cuts at: 1936 3049 1936 Size: 1113 4594 SapI GCTCTTCn'nnn_ Cuts at: 2182 2182 Size: 5707 SbfI CC_TGCA'GG Cuts at: 3059 3059 Size: 5707 ScaI AGT'ACT Cuts at: 3955 5667 3955 Size: 1712 3995 SfcI C'TryA_G Cuts at: 826 1045 3055 4190 4871 5062 826 Size: 219 2010 1135 681 191 1471 Fragments arranged by size: 2010 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5707 SmaI CCC'GGG Cuts at: 1872 2047 3034 1872 Size: 175 987 4545 Fragments arranged by size: 4545 987 175 SmlI C'TyrA_G Cuts at: 820 1017 1943 2830 3811 4712 4974 820 Size: 197 926 887 981 901 262 1553 Fragments arranged by size: 1553 981 926 901 887 262 197 SnaBI TAC'GTA Cuts at: 493 3498 493 Size: 3005 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5707 SphI G_CATG'C Cuts at: 1132 2168 3065 1132 Size: 1036 897 3774 Fragments arranged by size: 3774 1036 897 SspI AAT'ATT Cuts at: 5 52 3631 5 Size: 47 3579 2081 Fragments arranged by size: 3579 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5707 StyI C'CwwG_G Cuts at: 513 1065 2004 2857 2910 3146 513 Size: 552 939 853 53 236 3074 Fragments arranged by size: 3074 939 853 552 236 53 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3734 3751 3904 4089 4534 325 Size: 1255 2154 17 153 185 445 1498 Fragments arranged by size: 2154 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2134 2275 3953 Size: 274 80 33 51 1443 157 141 1678 Cuts at: 3953 5665 96 Size: 1712 138 Fragments arranged by size: 1712 1678 1443 274 157 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5667 5699 5667 Size: 32 5675 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5707 XbaI T'CTAG_A Cuts at: 3043 3043 Size: 5707 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5707 XmaI C'CCGG_G Cuts at: 1870 2045 3032 1870 Size: 175 987 4545 Fragments arranged by size: 4545 987 175 XmnI GAAnn'nnTTC Cuts at: 3836 3836 Size: 5707 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3515 276 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI