Restriction map for
pFN21AE0413
(Linear) (Six-base) MAPSORT of: KIEE0413.seq Check: 1154 from: 1 to: 1002
KIEE0413.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AflII C'TTAA_G
Cuts at: 0 825 1002
Size: 825 177
AflIII A'CryG_T
Cuts at: 0 666 1002
Size: 666 336
AhdI GACnn_n'nnGTC
Cuts at: 0 991 1002
Size: 991 11
AlwNI CAG_nnn'CTG
Cuts at: 0 322 376 592 1002
Size: 322 54 216 410
Fragments arranged by size:
410 322 216 54
ApoI r'AATT_y
Cuts at: 0 139 196 354 468 1002
Size: 139 57 158 114 534
Fragments arranged by size:
534 158 139 114 57
AvaI C'yCGr_G
Cuts at: 0 40 1002
Size: 40 962
AvrII C'CTAG_G
Cuts at: 0 852 1002
Size: 852 150
BamHI G'GATC_C
Cuts at: 0 720 1002
Size: 720 282
BanI G'GyrC_C
Cuts at: 0 103 1002
Size: 103 899
BanII G_rGCy'C
Cuts at: 0 23 1002
Size: 23 979
BbsI GAAGACnn'nnnn_
Cuts at: 0 297 455 1002
Size: 297 158 547
Fragments arranged by size:
547 297 158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 565 599 1002
Size: 565 34 403
Fragments arranged by size:
565 403 34
BlpI GC'TnA_GC
Cuts at: 0 878 1002
Size: 878 124
BmrI ACTGGGnnnn_n'
Cuts at: 0 119 1002
Size: 119 883
BmtI G_CTAG'C
Cuts at: 0 703 1002
Size: 703 299
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 406 893 1002
Size: 406 487 109
Fragments arranged by size:
487 406 109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 1002
Size: 532 470
BsaI GGTCTCn'nnnn_
Cuts at: 0 895 1002
Size: 895 107
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 518 548 1002
Size: 518 30 454
Fragments arranged by size:
518 454 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 1002
Size: 76 926
BseYI C'CCAG_C
Cuts at: 0 814 1002
Size: 814 188
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 605 631 1002
Size: 299 306 26 371
Fragments arranged by size:
371 306 299 26
BsiHKAI G_wGCw'C
Cuts at: 0 23 861 1002
Size: 23 838 141
Fragments arranged by size:
838 141 23
BsmI GAATG_Cn'
Cuts at: 0 719 1002
Size: 719 283
Bsp1286I G_dGCh'C
Cuts at: 0 23 861 1002
Size: 23 838 141
Fragments arranged by size:
838 141 23
BspHI T'CATG_A
Cuts at: 0 246 1002
Size: 246 756
BsrDI GCAATG_nn'
Cuts at: 0 762 1002
Size: 762 240
BsrFI r'CCGG_y
Cuts at: 0 692 1002
Size: 692 310
BsrGI T'GTAC_A
Cuts at: 0 129 1002
Size: 129 873
BstBI TT'CG_AA
Cuts at: 0 933 1002
Size: 933 69
BstYI r'GATC_y
Cuts at: 0 720 1002
Size: 720 282
BtsI GCAGTG_nn'
Cuts at: 0 593 1002
Size: 593 409
DraIII CAC_nnn'GTG
Cuts at: 0 689 1002
Size: 689 313
DrdI GACnn_nn'nnGTC
Cuts at: 0 847 1002
Size: 847 155
EaeI y'GGCC_r
Cuts at: 0 680 915 1002
Size: 680 235 87
Fragments arranged by size:
680 235 87
EarI CTCTTCn'nnn_
Cuts at: 0 177 703 1002
Size: 177 526 299
Fragments arranged by size:
526 299 177
EcoICRI GAG'CTC
Cuts at: 0 21 1002
Size: 21 981
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 1002
Size: 532 470
EcoNI CCTnn'n_nnAGG
Cuts at: 0 851 1002
Size: 851 151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 756 970 1002
Size: 756 214 32
Fragments arranged by size:
756 214 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 977 1002
Size: 310 32 635 25
Fragments arranged by size:
635 310 32 25
HincII GTy'rAC
Cuts at: 0 72 1002
Size: 72 930
HpaI GTT'AAC
Cuts at: 0 72 1002
Size: 72 930
MfeI C'AATT_G
Cuts at: 0 791 1002
Size: 791 211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 360 1002
Size: 197 163 642
Fragments arranged by size:
642 197 163
MscI TGG'CCA
Cuts at: 0 682 917 1002
Size: 682 235 85
Fragments arranged by size:
682 235 85
MspA1I CmG'CkG
Cuts at: 0 373 1002
Size: 373 629
NaeI GCC'GGC
Cuts at: 0 694 1002
Size: 694 308
NgoMIV G'CCGG_C
Cuts at: 0 692 1002
Size: 692 310
NheI G'CTAG_C
Cuts at: 0 699 1002
Size: 699 303
NspI r_CATG'y
Cuts at: 0 163 670 788 1002
Size: 163 507 118 214
Fragments arranged by size:
507 214 163 118
PciI A'CATG_T
Cuts at: 0 666 1002
Size: 666 336
PfoI T'CCnGG_A
Cuts at: 0 509 1002
Size: 509 493
PvuII CAG'CTG
Cuts at: 0 373 1002
Size: 373 629
SacI G_AGCT'C
Cuts at: 0 23 1002
Size: 23 979
SapI GCTCTTCn'nnn_
Cuts at: 0 177 1002
Size: 177 825
SmaI CCC'GGG
Cuts at: 0 42 1002
Size: 42 960
SmlI C'TyrA_G
Cuts at: 0 825 1002
Size: 825 177
SphI G_CATG'C
Cuts at: 0 163 1002
Size: 163 839
StyI C'CwwG_G
Cuts at: 0 852 905 1002
Size: 852 53 97
Fragments arranged by size:
852 97 53
TatI w'GTAC_w
Cuts at: 0 129 270 1002
Size: 129 141 732
Fragments arranged by size:
732 141 129
XmaI C'CCGG_G
Cuts at: 0 40 1002
Size: 40 962
Enzymes that do cut:
AflII AflIII AhdI AlwNI ApoI AvaI AvrII BamHI
BanI BanII BbsI BcgI BlpI BmrI BmtI BplI
BpmI BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BsrDI BsrFI BsrGI BstBI BstYI BtsI
DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI FalI
Hin4I HincII HpaI MfeI MmeI MscI MspA1I NaeI
NgoMIV NheI NspI PciI PfoI PvuII SacI SapI
SmaI SmlI SphI StyI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AgeI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
BaeI BbeI BbvCI BciVI BclI BfrBI BglI BglII
Bme1580I BmgBI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BssHII BssSI
BstAPI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI EagI EciI EcoO109I EcoRI EcoRV FseI
FspI FspAI HaeII HindIII KasI KpnI MluI MslI
NarI NcoI NdeI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmnI ZraI