Restriction map for pFN21AA0705
 (Circular) (Six-base) MAPSORT of: pFN21AA0705.seq  Check: 1260  from: 1  to: 9028



pFN21AA0705  9028 bp

 With 182 enzymes: SgfI * 

                        January 31, 2011 17:47  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2777    3346    4174    2777 
   Size:        569     828    7631

  Fragments arranged by size: 

                7631     828     569
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    6838     278 
   Size:         53      83     186     936    5302    2468

  Fragments arranged by size: 

                5302    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2958    6371    1937 
   Size:       1021    3413    4594

  Fragments arranged by size: 

                4594    3413    1021
 
Acc65I G'GTAC_C

Cuts at:   2555    6349    2555 
   Size:       3794    5234
 
AclI AA'CG_TT

Cuts at:   1563    7155    7528    1563 
   Size:       5592     373    3063

  Fragments arranged by size: 

                5592    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2120    2363    2658    2873    2910    3573    4315    4707 
   Size:        488     243     295     215      37     663     742     392

Cuts at:   4707    5126    7091    8475    8743    1632 
   Size:        419    1965    1384     268    1917

  Fragments arranged by size: 

                1965    1917    1384     742     663     488     419     392
                 295     268     243     215      37
 
AfeI AGC'GCT

Cuts at:   5909    5909 
   Size:       9028
 
AflII C'TTAA_G

Cuts at:    820    1017    4001     820 
   Size:        197    2984    5847

  Fragments arranged by size: 

                5847    2984     197
 
AflIII A'CryG_T

Cuts at:   1203    7927    8904    1203 
   Size:       6724     977    1327

  Fragments arranged by size: 

                6724    1327     977
 
AgeI A'CCGG_T

Cuts at:   7859    7859 
   Size:       9028
 
AhdI GACnn_n'nnGTC

Cuts at:   3775    7757    3775 
   Size:       3982    5046
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       9028
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       9028
 
AlwNI CAG_nnn'CTG

Cuts at:   3973    4153    4595    6771    8343    3973 
   Size:        180     442    2176    1572    4658

  Fragments arranged by size: 

                4658    2176    1572     442     180
 
ApaI G_GGCC'C

Cuts at:   6209    6261    6209 
   Size:         52    8976
 
ApaLI G'TGCA_C

Cuts at:   7085    8241    7085 
   Size:       1156    7872
 
ApoI r'AATT_y

Cuts at:   1493    6337    6563    6715    8824    1493 
   Size:       4844     226     152    2109    1697

  Fragments arranged by size: 

                4844    2109    1697     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3432    7582     160 
   Size:        872    2400    4150    1606

  Fragments arranged by size: 

                4150    2400    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       9028
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3246    4340    5891    5945    5975    6011    6109 
   Size:         73    1303    1094    1551      54      30      36      98

Cuts at:   6109    6136    6217    6353    1870 
   Size:         27      81     136    4545

  Fragments arranged by size: 

                4545    1551    1303    1094     136      98      81      73
                  54      36      30      27
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2539    2572    2539 
   Size:         33    8995
 
BamHI G'GATC_C

Cuts at:   2998    4796    4884    6358    6725    2998 
   Size:       1798      88    1474     367    5301

  Fragments arranged by size: 

                5301    1798    1474     367      88
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2108    2416    2555    2789    3574 
   Size:        325     217     627     321     308     139     234     785

Cuts at:   3574    3836    5014    5953    6007    6064    6224    6268    6308 
   Size:        262    1178     939      54      57     160      44      40

Cuts at:   6308    6349    7804     618 
   Size:         41    1455    1842

  Fragments arranged by size: 

                1842    1455    1178     939     785     627     325     321
                 308     262     234     217     160     139      57      54
                  44      41      40
 
BanII G_rGCy'C

Cuts at:   1391    2235    4184    4239    4397    4566    4998    6018    6086 
   Size:        844    1949      55     158     169     432    1020      68

Cuts at:   6086    6093    6209    6261    6347    1391 
   Size:          7     116      52      86    4072

  Fragments arranged by size: 

                4072    1949    1020     844     432     169     158     116
                  86      68      55      52       7
 
BbeI G_GCGC'C

Cuts at:   2112    5957    6011    6068    6228    6312    2112 
   Size:       3845      54      57     160      84    4828

  Fragments arranged by size: 

                4828    3845     160      84      57      54
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2547    2781    2898    3561    3631    5623     928 
   Size:        971     648     234     117     663      70    1992    4333

  Fragments arranged by size: 

                4333    1992     971     663     648     234     117      70
            
 
BbvCI CC'TCA_GC

Cuts at:   3278    3278 
   Size:       9028
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2372    2406    7219    7253    1903 
   Size:         34     435      34    4813      34    3678

  Fragments arranged by size: 

                4813    3678     435      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3759    4609    6920    8130    3759 
   Size:        850    2311    1210    4657

  Fragments arranged by size: 

                4657    2311    1210     850
 
BclI T'GATC_A

Cuts at:   1548    2259    1548 
   Size:        711    8317
 
BfrBI ATG'CAT

Cuts at:   3254    3254 
   Size:       9028
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2088    5999    6141 
   Size:        107     122      71     631     605     416    3911     142

Cuts at:   6141    7639     136 
   Size:       1498    1525

  Fragments arranged by size: 

                3911    1525    1498     631     605     416     142     122
                 107      71
 
BglII A'GATC_T

Cuts at:   9023    9023 
   Size:       9028
 
BlpI GC'TnA_GC

Cuts at:   4396    6445    4396 
   Size:       2049    6979
 
Bme1580I G_kGCm'C

Cuts at:   1790    3841    4815    6209    6261    7089    8245    1790 
   Size:       2051     974    1394      52     828    1156    2573

  Fragments arranged by size: 

                2573    2051    1394    1156     974     828      52
 
BmgBI CAC'GTC

Cuts at:   1318    2248    1318 
   Size:        930    8098
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2935    3253    4659 
   Size:        416      53      83     399      24    1518     318    1406

Cuts at:   4659    6759    7717     442 
   Size:       2100     958    1753

  Fragments arranged by size: 

                2100    1753    1518    1406     958     416     399     318
                  83      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       9028
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2824    3621    2824 
   Size:        797    8231
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2225    2478    3012    3591    5631    5704    6182    7688 
   Size:        986     253     534     579    2040      73     478    1506

Cuts at:   7688    1239 
   Size:       2579

  Fragments arranged by size: 

                2579    2040    1506     986     579     534     478     253
                  73
 
Bpu10I CC'TnA_GC

Cuts at:   3278    3974    3278 
   Size:        696    8332
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2273    5621    7153    8018    8316    2273 
   Size:       3348    1532     865     298    2985

  Fragments arranged by size: 

                3348    2985    1532     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2210    4872    7691     882 
   Size:        617     711    2662    2819    2219

  Fragments arranged by size: 

                2819    2662    2219     711     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3747    6819     493 
   Size:        711    2543    3072    2702

  Fragments arranged by size: 

                3072    2702    2543     711
 
BsaBI GATnn'nnATC

Cuts at:   4299    9022    4299 
   Size:       4723    4305
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2109    4273    5727    5954 
   Size:         53      83     186     936     576    2164    1454     227

Cuts at:   5954    6008    6065    6225    6309    6835    7217    7852     275 
   Size:         54      57     160      84     526     382     635    1451

  Fragments arranged by size: 

                2164    1454    1451     936     635     576     526     382
                 227     186     160      84      83      57      54      53
            
 
BsaWI w'CCGG_w

Cuts at:   3373    4881    7461    7859    8133    8280    8691    3373 
   Size:       1508    2580     398     274     147     411    3710

  Fragments arranged by size: 

                3710    2580    1508     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2298    2328    2733    2763    4166 
   Size:         30     165      30     895      30     405      30    1403

Cuts at:   4166    4196    1178 
   Size:         30    6010

  Fragments arranged by size: 

                6010    1403     895     405     165      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2207    2717    2861    4062    5006    5963    1186 
   Size:       1021     510     144    1201     944     957    4251

  Fragments arranged by size: 

                4251    1201    1021     957     944     510     144
 
BseYI C'CCAG_C

Cuts at:   2086    2811    3063    5018    5082    5092    5118    6290    8231 
   Size:        725     252    1955      64      10      26    1172    1941

Cuts at:   8231    8727    2086 
   Size:        496    2387

  Fragments arranged by size: 

                2387    1955    1941    1172     725     496     252      64
                  26      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2805    2808    3695    1163 
   Size:        573    1069       3     887    6496

  Fragments arranged by size: 

                6496    1069     887     573       3
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3698    5939    6203    6318    6478    7239 
   Size:        275     186    1697    2241     264     115     160     761

Cuts at:   7239    8267    1540 
   Size:       1028    2301

  Fragments arranged by size: 

                2301    2241    1697    1028     761     275     264     186
                 160     115
 
BsiHKAI G_wGCw'C

Cuts at:   2518    4184    4239    4998    6093    7089    7174    8245    2518 
   Size:       1666      55     759    1095     996      85    1071    3301

  Fragments arranged by size: 

                3301    1666    1095    1071     996     759      85      55
            
 
BsmI GAATG_Cn'

Cuts at:   5442    6539    6632    5442 
   Size:       1097      93    7838

  Fragments arranged by size: 

                7838    1097      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   3327    5734    3327 
   Size:       2407    6621
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2235    2518    3841    4184    4239    4397    4566 
   Size:        399     445     283    1323     343      55     158     169

Cuts at:   4566    4815    4998    6018    6086    6093    6209    6261    6347 
   Size:        249     183    1020      68       7     116      52      86

Cuts at:   6347    7089    7174    8245    1391 
   Size:        742      85    1071    2174

  Fragments arranged by size: 

                2174    1323    1071    1020     742     445     399     343
                 283     249     183     169     158     116      86      85
                  68      55      52       7
 
BspEI T'CCGG_A

Cuts at:   3373    8691    3373 
   Size:       5318    3710
 
BspHI T'CATG_A

Cuts at:   6917    8647    6917 
   Size:       1730    7298
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2777    3346    4161    4174    4510    4946    6383     844 
   Size:       1933     569     815      13     336     436    1437    3489

  Fragments arranged by size: 

                3489    1933    1437     815     569     436     336      13
            
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2172    6915    1394 
   Size:        429     349    4743    3507

  Fragments arranged by size: 

                4743    3507     429     349
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2912    4383    4519    4678    7523    7697      65 
   Size:       1183    1664    1471     136     159    2845     174    1396

  Fragments arranged by size: 

                2845    1664    1471    1396    1183     174     159     136
            
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4463    5981    6096    6121    6131    7672    7859 
   Size:        110    2653    1518     115      25      10    1541     187

Cuts at:   7859    1700 
   Size:       2869

  Fragments arranged by size: 

                2869    2653    1541    1518     187     115     110      25
                  10
 
BsrGI T'GTAC_A

Cuts at:     96    2832    3964      96 
   Size:       2736    1132    5160

  Fragments arranged by size: 

                5160    2736    1132
 
BssHII G'CGCG_C

Cuts at:   1925    6156    6185    6187    6233    1925 
   Size:       4231      29       2      46    4720

  Fragments arranged by size: 

                4720    4231      46      29       2
 
BssSI C'ACGA_G

Cuts at:   7088    8100    7088 
   Size:       1012    8016
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3718    4153     794 
   Size:       2924     435    5669

  Fragments arranged by size: 

                5669    2924     435
 
BstBI TT'CG_AA

Cuts at:   7832    7832 
   Size:       9028
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       9028
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3063    4723    3063 
   Size:       1660    7368
 
BstYI r'GATC_y

Cuts at:   1972    2998    4796    4884    6358    6725    7110    7127    8579 
   Size:       1026    1798      88    1474     367     385      17    1452

Cuts at:   8579    8665    8677    9023    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1798    1474    1452    1026     385     367     346
                  88      86      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2700    2794    2844    4986    2700 
   Size:         94      50    2142    6742

  Fragments arranged by size: 

                6742    2142      94      50
 
BtgI C'CryG_G

Cuts at:    513    1065    2223    4064    4214    4389    5040     513 
   Size:        552    1158    1841     150     175     651    4501

  Fragments arranged by size: 

                4501    1841    1158     651     552     175     150
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    5098     487 
   Size:        683      56    3872    4417

  Fragments arranged by size: 

                4417    3872     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2266    4630    4899    6544    7338    7358    2266 
   Size:       2364     269    1645     794      20    3936

  Fragments arranged by size: 

                3936    2364    1645     794     269      20
 
ClaI AT'CG_AT

Cuts at:   1554    3075    1554 
   Size:       1521    7507
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    8993
 
DraI TTT'AAA

Cuts at:   6332    6332 
   Size:       9028
 
DraIII CAC_nnn'GTG

Cuts at:   4625    4625 
   Size:       9028
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    3670    8035     809 
   Size:        507    2354    4365    1802

  Fragments arranged by size: 

                4365    2354    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2253    3695    4137 
   Size:         54    1425      50     275      18     423    1442     442

Cuts at:   4137    4411    4900    5872    5936    5962    6200    6315    6475 
   Size:        274     489     972      64      26     238     115     160

Cuts at:   6475    7364    8993       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1442    1425     972     889     489     442     423
                 275     274     238     160     115      64      54      50
                  43      26      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3695    5936    6200    6315    6475    1537 
   Size:        275    1883    2241     264     115     160    4090

  Fragments arranged by size: 

                4090    2241    1883     275     264     160     115
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2645    2703    2709    3027    3245    6958    1353 
   Size:       1292      58       6     318     218    3713    3423

  Fragments arranged by size: 

                3713    3423    1292     318     218      58       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    6050    7615    7989    8135     387 
   Size:       1064     138    4461    1565     374     146    1280

  Fragments arranged by size: 

                4461    1565    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   4182    4237    4996    6091    4182 
   Size:         55     759    1095    7119

  Fragments arranged by size: 

                7119    1095     759      55
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2120    2225    2363    2478    2658    2873    2910 
   Size:        393     488     105     138     115     180     215      37

Cuts at:   2910    3012    3573    3591    4315    4707    5126    5631    5704 
   Size:        102     561      18     724     392     419     505      73

Cuts at:   5704    6182    7091    7688    8475    8743    1239 
   Size:        478     909     597     787     268    1524

  Fragments arranged by size: 

                1524     909     787     724     597     561     505     488
                 478     419     393     392     268     215     180     138
                 115     105     102      73      37      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2138    2138 
   Size:       9028
 
EcoO109I rG'GnC_Cy

Cuts at:   2275    2452    3214    5656    6194    6257    6258    2275 
   Size:        177     762    2442     538      63       1    5045

  Fragments arranged by size: 

                5045    2442     762     538     177      63       1
 
EcoRI G'AATT_C

Cuts at:   6337    6715    6337 
   Size:        378    8650
 
EcoRV GAT'ATC

Cuts at:   5824    5824 
   Size:       9028
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2550    3827    4044    2550 
   Size:       1277     217    7534

  Fragments arranged by size: 

                7534    1277     217
 
FspI TGC'GCA

Cuts at:   7534    7534 
   Size:       9028
 
HaeII r_GCGC'y

Cuts at:   1678    2112    5890    5911    5957    6011    6068    6228    6312 
   Size:        434    3778      21      46      54      57     160      84

Cuts at:   6312    8175    1678 
   Size:       1863    2531

  Fragments arranged by size: 

                3778    2531    1863     434     160      84      57      54
                  46      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3306    3338    3761    3793    4027 
   Size:         32     135      32    1734      32     423      32     234

Cuts at:   4027    4059    4781    4813    4869    4901    5526    5558    7669 
   Size:         32     722      32      56      32     625      32    2111

Cuts at:   7669    7701    7743    7775    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    2111    1734     722     625     423     234     135
                  56      42      32      32      32      32      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3106    3511    4525    6372    6618     669 
   Size:        988     281    1168     405    1014    1847     246    3079

  Fragments arranged by size: 

                3079    1847    1168    1014     988     405     281     246
            
 
HindIII A'AGCT_T

Cuts at:    748    2014     748 
   Size:       1266    7762
 
HpaI GTT'AAC

Cuts at:   6618    6618 
   Size:       9028
 
KasI G'GCGC_C

Cuts at:   2108    5953    6007    6064    6224    6308    2108 
   Size:       3845      54      57     160      84    4828

  Fragments arranged by size: 

                4828    3845     160      84      57      54
 
KpnI G_GTAC'C

Cuts at:   2559    6353    2559 
   Size:       3794    5234
 
MfeI C'AATT_G

Cuts at:   6627    6627 
   Size:       9028
 
MluI A'CGCG_T

Cuts at:   8904    8904 
   Size:       9028
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2169    5236    5346    5808    8142    8326    1305 
   Size:        864    3067     110     462    2334     184    2007

  Fragments arranged by size: 

                3067    2334    2007     864     462     184     110
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    4902      10 
   Size:         54    1425     343    3070    4136

  Fragments arranged by size: 

                4136    3070    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2055    3459    3465    3633    4388    4479    4916 
   Size:        648     889    1404       6     168     755      91     437

Cuts at:   4916    6986    7345    7504     518 
   Size:       2070     359     159    2042

  Fragments arranged by size: 

                2070    2042    1404     889     755     648     437     359
                 168     159      91       6
 
MspA1I CmG'CkG

Cuts at:   1606    2086    3883    5146    5248    5696    6153    6444    7121 
   Size:        480    1797    1263     102     448     457     291     677

Cuts at:   7121    8269    8514    1606 
   Size:       1148     245    2120

  Fragments arranged by size: 

                2120    1797    1263    1148     677     480     457     448
                 291     245     102
 
NaeI GCC'GGC

Cuts at:   4465    5983    6098    6123    6133    4465 
   Size:       1518     115      25      10    7360

  Fragments arranged by size: 

                7360    1518     115      25      10
 
NarI GG'CG_CC

Cuts at:   2109    5954    6008    6065    6225    6309    2109 
   Size:       3845      54      57     160      84    4828

  Fragments arranged by size: 

                4828    3845     160      84      57      54
 
NcoI C'CATG_G

Cuts at:    513    1065    4064    4389    5040     513 
   Size:        552    2999     325     651    4501

  Fragments arranged by size: 

                4501    2999     651     552     325
 
NdeI CA'TA_TG

Cuts at:    387    3058    4231     387 
   Size:       2671    1173    5184

  Fragments arranged by size: 

                5184    2671    1173
 
NgoMIV G'CCGG_C

Cuts at:   4463    5981    6096    6121    6131    4463 
   Size:       1518     115      25      10    7360

  Fragments arranged by size: 

                7360    1518     115      25      10
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       9028
 
NotI GC'GGCC_GC

Cuts at:   6475    6475 
   Size:       9028
 
NsiI A_TGCA'T

Cuts at:   3256    3256 
   Size:       9028
 
NspI r_CATG'y

Cuts at:   1132    1229    3181    4921    5585    6386    7931    1132 
   Size:         97    1952    1740     664     801    1545    2229

  Fragments arranged by size: 

                2229    1952    1740    1545     801     664      97
 
PasI CC'CwG_GG

Cuts at:   1348    3841    1348 
   Size:       2493    6535
 
PciI A'CATG_T

Cuts at:   7927    7927 
   Size:       9028
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    3728    1108 
   Size:       2620    6408
 
PfoI T'CCnGG_A

Cuts at:   2617    3144    2617 
   Size:        527    8501
 
PmeI GTTT'AAAC

Cuts at:   6332    6332 
   Size:       9028
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   7097    7129    8610    8642    7097 
   Size:         32    1481      32    7483

  Fragments arranged by size: 

                7483    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2275    2452    5656    6194    2275 
   Size:        177    3204     538    5109

  Fragments arranged by size: 

                5109    3204     538     177
 
PshAI GACnn'nnGTC

Cuts at:   1613    2328    1613 
   Size:        715    8313
 
PsiI TTA'TAA

Cuts at:   4284    6598    4284 
   Size:       2314    6714
 
PspOMI G'GGCC_C

Cuts at:   6205    6257    6205 
   Size:         52    8976
 
PspXI vC'TCGA_Gb

Cuts at:   5945    5945 
   Size:       9028
 
PstI C_TGCA'G

Cuts at:    830    3213    3357    3388    4713    4852    6380     830 
   Size:       2383     144      31    1325     139    1528    3478

  Fragments arranged by size: 

                3478    2383    1528    1325     144     139      31
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       9028
 
PvuII CAG'CTG

Cuts at:   2086    5146    2086 
   Size:       3060    5968
 
SacI G_AGCT'C

Cuts at:   4184    4239    4998    6093    4184 
   Size:         55     759    1095    7119

  Fragments arranged by size: 

                7119    1095     759      55
 
SalI G'TCGA_C

Cuts at:   1936    6370    1936 
   Size:       4434    4594
 
SanDI GG'GwC_CC

Cuts at:   6194    6194 
   Size:       9028
 
SbfI CC_TGCA'GG

Cuts at:   3213    6380    3213 
   Size:       3167    5861
 
ScaI AGT'ACT

Cuts at:   7276    8988    7276 
   Size:       1712    7316
 
SexAI A'CCwGG_T

Cuts at:   3513    3513 
   Size:       9028
 
SfcI C'TryA_G

Cuts at:    826    1045    3164    3209    3353    3384    3873    4709    4848 
   Size:        219    2119      45     144      31     489     836     139

Cuts at:   4848    5233    5302    5326    5371    6376    7511    8192    8383 
   Size:        385      69      24      45    1005    1135     681     191

Cuts at:   8383     826 
   Size:       1471

  Fragments arranged by size: 

                2119    1471    1135    1005     836     681     489     385
                 219     191     144     139      69      45      45      31
                  24
 
SfoI GGC'GCC

Cuts at:   2110    5955    6009    6066    6226    6310    2110 
   Size:       3845      54      57     160      84    4828

  Fragments arranged by size: 

                4828    3845     160      84      57      54
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       9028
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    6121    1700 
   Size:       4421    4607
 
SmaI CCC'GGG

Cuts at:   1872    3248    6013    6111    6138    6219    6355    1872 
   Size:       1376    2765      98      27      81     136    4545

  Fragments arranged by size: 

                4545    2765    1376     136      98      81      27
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2288    4001    5636    5945    7132    8033 
   Size:        197     926     345    1713    1635     309    1187     901

Cuts at:   8033    8295     820 
   Size:        262    1553

  Fragments arranged by size: 

                1713    1635    1553    1187     926     901     345     309
                 262     197
 
SnaBI TAC'GTA

Cuts at:    493    6819     493 
   Size:       6326    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       9028
 
SphI G_CATG'C

Cuts at:   1132    6386    1132 
   Size:       5254    3774
 
SrfI GCCC'GGGC

Cuts at:   6013    6138    6013 
   Size:        125    8903
 
SspI AAT'ATT

Cuts at:      5      52    6952       5 
   Size:         47    6900    2081

  Fragments arranged by size: 

                6900    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2722    4205    4786    1517 
   Size:       1205    1483     581    5759

  Fragments arranged by size: 

                5759    1483    1205     581
 
StyI C'CwwG_G

Cuts at:    513    1065    3448    4064    4389    5040    5295    6467     513 
   Size:        552    2383     616     325     651     255    1172    3074

  Fragments arranged by size: 

                3074    2383    1172     651     616     552     325     255
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3815    4107    4437    4619    5032    7055    7072 
   Size:       1255    2235     292     330     182     413    2023      17

Cuts at:   7072    7225    7410    7855     325 
   Size:        153     185     445    1498

  Fragments arranged by size: 

                2235    2023    1498    1255     445     413     330     292
                 185     182     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2832    3651    3964 
   Size:        274      80      33      51    1443     855     819     313

Cuts at:   3964    7274    8986      96 
   Size:       3310    1712     138

  Fragments arranged by size: 

                3310    1712    1443     855     819     313     274     138
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3744    3776    3870    3902    4166    4198    5607    5639    8988 
   Size:         32      94      32     264      32    1409      32    3349

Cuts at:   8988    9020    3744 
   Size:         32    3752

  Fragments arranged by size: 

                3752    3349    1409     264      94      32      32      32
                  32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       9028
 
XbaI T'CTAG_A

Cuts at:   6364    6364 
   Size:       9028
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3191    5814    3191 
   Size:       2623    6405
 
XhoI C'TCGA_G

Cuts at:   1943    5945    1943 
   Size:       4002    5026
 
XmaI C'CCGG_G

Cuts at:   1870    3246    6011    6109    6136    6217    6353    1870 
   Size:       1376    2765      98      27      81     136    4545

  Fragments arranged by size: 

                4545    2765    1376     136      98      81      27
 
XmnI GAAnn'nnTTC

Cuts at:   7157    7157 
   Size:       9028
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    6836     276 
   Size:         53      83     186     936    5302    2468

  Fragments arranged by size: 

                5302    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NsiI
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI    PvuII     SacI
     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AloI     AscI    AvrII    BsiWI  BstZ17I     FseI    FspAI     NruI
     PacI     PmlI     PsrI    RsrII    SacII     SapI     SfiI     SwaI
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