(Circular) (Six-base) MAPSORT of: pFN21AA0705.seq Check: 1260 from: 1 to: 9028 pFN21AA0705 9028 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2777 3346 4174 2777 Size: 569 828 7631 Fragments arranged by size: 7631 828 569 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6838 278 Size: 53 83 186 936 5302 2468 Fragments arranged by size: 5302 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2958 6371 1937 Size: 1021 3413 4594 Fragments arranged by size: 4594 3413 1021 Acc65I G'GTAC_C Cuts at: 2555 6349 2555 Size: 3794 5234 AclI AA'CG_TT Cuts at: 1563 7155 7528 1563 Size: 5592 373 3063 Fragments arranged by size: 5592 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2120 2363 2658 2873 2910 3573 4315 4707 Size: 488 243 295 215 37 663 742 392 Cuts at: 4707 5126 7091 8475 8743 1632 Size: 419 1965 1384 268 1917 Fragments arranged by size: 1965 1917 1384 742 663 488 419 392 295 268 243 215 37 AfeI AGC'GCT Cuts at: 5909 5909 Size: 9028 AflII C'TTAA_G Cuts at: 820 1017 4001 820 Size: 197 2984 5847 Fragments arranged by size: 5847 2984 197 AflIII A'CryG_T Cuts at: 1203 7927 8904 1203 Size: 6724 977 1327 Fragments arranged by size: 6724 1327 977 AgeI A'CCGG_T Cuts at: 7859 7859 Size: 9028 AhdI GACnn_n'nnGTC Cuts at: 3775 7757 3775 Size: 3982 5046 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9028 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9028 AlwNI CAG_nnn'CTG Cuts at: 3973 4153 4595 6771 8343 3973 Size: 180 442 2176 1572 4658 Fragments arranged by size: 4658 2176 1572 442 180 ApaI G_GGCC'C Cuts at: 6209 6261 6209 Size: 52 8976 ApaLI G'TGCA_C Cuts at: 7085 8241 7085 Size: 1156 7872 ApoI r'AATT_y Cuts at: 1493 6337 6563 6715 8824 1493 Size: 4844 226 152 2109 1697 Fragments arranged by size: 4844 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3432 7582 160 Size: 872 2400 4150 1606 Fragments arranged by size: 4150 2400 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9028 AvaI C'yCGr_G Cuts at: 1870 1943 3246 4340 5891 5945 5975 6011 6109 Size: 73 1303 1094 1551 54 30 36 98 Cuts at: 6109 6136 6217 6353 1870 Size: 27 81 136 4545 Fragments arranged by size: 4545 1551 1303 1094 136 98 81 73 54 36 30 27 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2539 2572 2539 Size: 33 8995 BamHI G'GATC_C Cuts at: 2998 4796 4884 6358 6725 2998 Size: 1798 88 1474 367 5301 Fragments arranged by size: 5301 1798 1474 367 88 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2108 2416 2555 2789 3574 Size: 325 217 627 321 308 139 234 785 Cuts at: 3574 3836 5014 5953 6007 6064 6224 6268 6308 Size: 262 1178 939 54 57 160 44 40 Cuts at: 6308 6349 7804 618 Size: 41 1455 1842 Fragments arranged by size: 1842 1455 1178 939 785 627 325 321 308 262 234 217 160 139 57 54 44 41 40 BanII G_rGCy'C Cuts at: 1391 2235 4184 4239 4397 4566 4998 6018 6086 Size: 844 1949 55 158 169 432 1020 68 Cuts at: 6086 6093 6209 6261 6347 1391 Size: 7 116 52 86 4072 Fragments arranged by size: 4072 1949 1020 844 432 169 158 116 86 68 55 52 7 BbeI G_GCGC'C Cuts at: 2112 5957 6011 6068 6228 6312 2112 Size: 3845 54 57 160 84 4828 Fragments arranged by size: 4828 3845 160 84 57 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2547 2781 2898 3561 3631 5623 928 Size: 971 648 234 117 663 70 1992 4333 Fragments arranged by size: 4333 1992 971 663 648 234 117 70 BbvCI CC'TCA_GC Cuts at: 3278 3278 Size: 9028 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2372 2406 7219 7253 1903 Size: 34 435 34 4813 34 3678 Fragments arranged by size: 4813 3678 435 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3759 4609 6920 8130 3759 Size: 850 2311 1210 4657 Fragments arranged by size: 4657 2311 1210 850 BclI T'GATC_A Cuts at: 1548 2259 1548 Size: 711 8317 BfrBI ATG'CAT Cuts at: 3254 3254 Size: 9028 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2088 5999 6141 Size: 107 122 71 631 605 416 3911 142 Cuts at: 6141 7639 136 Size: 1498 1525 Fragments arranged by size: 3911 1525 1498 631 605 416 142 122 107 71 BglII A'GATC_T Cuts at: 9023 9023 Size: 9028 BlpI GC'TnA_GC Cuts at: 4396 6445 4396 Size: 2049 6979 Bme1580I G_kGCm'C Cuts at: 1790 3841 4815 6209 6261 7089 8245 1790 Size: 2051 974 1394 52 828 1156 2573 Fragments arranged by size: 2573 2051 1394 1156 974 828 52 BmgBI CAC'GTC Cuts at: 1318 2248 1318 Size: 930 8098 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2935 3253 4659 Size: 416 53 83 399 24 1518 318 1406 Cuts at: 4659 6759 7717 442 Size: 2100 958 1753 Fragments arranged by size: 2100 1753 1518 1406 958 416 399 318 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9028 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2824 3621 2824 Size: 797 8231 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 2478 3012 3591 5631 5704 6182 7688 Size: 986 253 534 579 2040 73 478 1506 Cuts at: 7688 1239 Size: 2579 Fragments arranged by size: 2579 2040 1506 986 579 534 478 253 73 Bpu10I CC'TnA_GC Cuts at: 3278 3974 3278 Size: 696 8332 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2273 5621 7153 8018 8316 2273 Size: 3348 1532 865 298 2985 Fragments arranged by size: 3348 2985 1532 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2210 4872 7691 882 Size: 617 711 2662 2819 2219 Fragments arranged by size: 2819 2662 2219 711 617 BsaAI yAC'GTr Cuts at: 493 1204 3747 6819 493 Size: 711 2543 3072 2702 Fragments arranged by size: 3072 2702 2543 711 BsaBI GATnn'nnATC Cuts at: 4299 9022 4299 Size: 4723 4305 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2109 4273 5727 5954 Size: 53 83 186 936 576 2164 1454 227 Cuts at: 5954 6008 6065 6225 6309 6835 7217 7852 275 Size: 54 57 160 84 526 382 635 1451 Fragments arranged by size: 2164 1454 1451 936 635 576 526 382 227 186 160 84 83 57 54 53 BsaWI w'CCGG_w Cuts at: 3373 4881 7461 7859 8133 8280 8691 3373 Size: 1508 2580 398 274 147 411 3710 Fragments arranged by size: 3710 2580 1508 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2298 2328 2733 2763 4166 Size: 30 165 30 895 30 405 30 1403 Cuts at: 4166 4196 1178 Size: 30 6010 Fragments arranged by size: 6010 1403 895 405 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2207 2717 2861 4062 5006 5963 1186 Size: 1021 510 144 1201 944 957 4251 Fragments arranged by size: 4251 1201 1021 957 944 510 144 BseYI C'CCAG_C Cuts at: 2086 2811 3063 5018 5082 5092 5118 6290 8231 Size: 725 252 1955 64 10 26 1172 1941 Cuts at: 8231 8727 2086 Size: 496 2387 Fragments arranged by size: 2387 1955 1941 1172 725 496 252 64 26 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2805 2808 3695 1163 Size: 573 1069 3 887 6496 Fragments arranged by size: 6496 1069 887 573 3 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3698 5939 6203 6318 6478 7239 Size: 275 186 1697 2241 264 115 160 761 Cuts at: 7239 8267 1540 Size: 1028 2301 Fragments arranged by size: 2301 2241 1697 1028 761 275 264 186 160 115 BsiHKAI G_wGCw'C Cuts at: 2518 4184 4239 4998 6093 7089 7174 8245 2518 Size: 1666 55 759 1095 996 85 1071 3301 Fragments arranged by size: 3301 1666 1095 1071 996 759 85 55 BsmI GAATG_Cn' Cuts at: 5442 6539 6632 5442 Size: 1097 93 7838 Fragments arranged by size: 7838 1097 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3327 5734 3327 Size: 2407 6621 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2235 2518 3841 4184 4239 4397 4566 Size: 399 445 283 1323 343 55 158 169 Cuts at: 4566 4815 4998 6018 6086 6093 6209 6261 6347 Size: 249 183 1020 68 7 116 52 86 Cuts at: 6347 7089 7174 8245 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1323 1071 1020 742 445 399 343 283 249 183 169 158 116 86 85 68 55 52 7 BspEI T'CCGG_A Cuts at: 3373 8691 3373 Size: 5318 3710 BspHI T'CATG_A Cuts at: 6917 8647 6917 Size: 1730 7298 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2777 3346 4161 4174 4510 4946 6383 844 Size: 1933 569 815 13 336 436 1437 3489 Fragments arranged by size: 3489 1933 1437 815 569 436 336 13 BsrBI CCG'CTC Cuts at: 1394 1823 2172 6915 1394 Size: 429 349 4743 3507 Fragments arranged by size: 4743 3507 429 349 BsrDI GCAATG_nn' Cuts at: 65 1248 2912 4383 4519 4678 7523 7697 65 Size: 1183 1664 1471 136 159 2845 174 1396 Fragments arranged by size: 2845 1664 1471 1396 1183 174 159 136 BsrFI r'CCGG_y Cuts at: 1700 1810 4463 5981 6096 6121 6131 7672 7859 Size: 110 2653 1518 115 25 10 1541 187 Cuts at: 7859 1700 Size: 2869 Fragments arranged by size: 2869 2653 1541 1518 187 115 110 25 10 BsrGI T'GTAC_A Cuts at: 96 2832 3964 96 Size: 2736 1132 5160 Fragments arranged by size: 5160 2736 1132 BssHII G'CGCG_C Cuts at: 1925 6156 6185 6187 6233 1925 Size: 4231 29 2 46 4720 Fragments arranged by size: 4720 4231 46 29 2 BssSI C'ACGA_G Cuts at: 7088 8100 7088 Size: 1012 8016 BstAPI GCAn_nnn'nTGC Cuts at: 794 3718 4153 794 Size: 2924 435 5669 Fragments arranged by size: 5669 2924 435 BstBI TT'CG_AA Cuts at: 7832 7832 Size: 9028 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9028 BstXI CCAn_nnnn'nTGG Cuts at: 3063 4723 3063 Size: 1660 7368 BstYI r'GATC_y Cuts at: 1972 2998 4796 4884 6358 6725 7110 7127 8579 Size: 1026 1798 88 1474 367 385 17 1452 Cuts at: 8579 8665 8677 9023 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1798 1474 1452 1026 385 367 346 88 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2700 2794 2844 4986 2700 Size: 94 50 2142 6742 Fragments arranged by size: 6742 2142 94 50 BtgI C'CryG_G Cuts at: 513 1065 2223 4064 4214 4389 5040 513 Size: 552 1158 1841 150 175 651 4501 Fragments arranged by size: 4501 1841 1158 651 552 175 150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5098 487 Size: 683 56 3872 4417 Fragments arranged by size: 4417 3872 683 56 BtsI GCAGTG_nn' Cuts at: 2266 4630 4899 6544 7338 7358 2266 Size: 2364 269 1645 794 20 3936 Fragments arranged by size: 3936 2364 1645 794 269 20 ClaI AT'CG_AT Cuts at: 1554 3075 1554 Size: 1521 7507 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8993 DraI TTT'AAA Cuts at: 6332 6332 Size: 9028 DraIII CAC_nnn'GTG Cuts at: 4625 4625 Size: 9028 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3670 8035 809 Size: 507 2354 4365 1802 Fragments arranged by size: 4365 2354 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2253 3695 4137 Size: 54 1425 50 275 18 423 1442 442 Cuts at: 4137 4411 4900 5872 5936 5962 6200 6315 6475 Size: 274 489 972 64 26 238 115 160 Cuts at: 6475 7364 8993 8 Size: 889 1629 43 Fragments arranged by size: 1629 1442 1425 972 889 489 442 423 275 274 238 160 115 64 54 50 43 26 18 EagI C'GGCC_G Cuts at: 1537 1812 3695 5936 6200 6315 6475 1537 Size: 275 1883 2241 264 115 160 4090 Fragments arranged by size: 4090 2241 1883 275 264 160 115 EarI CTCTTCn'nnn_ Cuts at: 1353 2645 2703 2709 3027 3245 6958 1353 Size: 1292 58 6 318 218 3713 3423 Fragments arranged by size: 3713 3423 1292 318 218 58 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6050 7615 7989 8135 387 Size: 1064 138 4461 1565 374 146 1280 Fragments arranged by size: 4461 1565 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4182 4237 4996 6091 4182 Size: 55 759 1095 7119 Fragments arranged by size: 7119 1095 759 55 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2120 2225 2363 2478 2658 2873 2910 Size: 393 488 105 138 115 180 215 37 Cuts at: 2910 3012 3573 3591 4315 4707 5126 5631 5704 Size: 102 561 18 724 392 419 505 73 Cuts at: 5704 6182 7091 7688 8475 8743 1239 Size: 478 909 597 787 268 1524 Fragments arranged by size: 1524 909 787 724 597 561 505 488 478 419 393 392 268 215 180 138 115 105 102 73 37 18 EcoNI CCTnn'n_nnAGG Cuts at: 2138 2138 Size: 9028 EcoO109I rG'GnC_Cy Cuts at: 2275 2452 3214 5656 6194 6257 6258 2275 Size: 177 762 2442 538 63 1 5045 Fragments arranged by size: 5045 2442 762 538 177 63 1 EcoRI G'AATT_C Cuts at: 6337 6715 6337 Size: 378 8650 EcoRV GAT'ATC Cuts at: 5824 5824 Size: 9028 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2550 3827 4044 2550 Size: 1277 217 7534 Fragments arranged by size: 7534 1277 217 FspI TGC'GCA Cuts at: 7534 7534 Size: 9028 HaeII r_GCGC'y Cuts at: 1678 2112 5890 5911 5957 6011 6068 6228 6312 Size: 434 3778 21 46 54 57 160 84 Cuts at: 6312 8175 1678 Size: 1863 2531 Fragments arranged by size: 3778 2531 1863 434 160 84 57 54 46 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3306 3338 3761 3793 4027 Size: 32 135 32 1734 32 423 32 234 Cuts at: 4027 4059 4781 4813 4869 4901 5526 5558 7669 Size: 32 722 32 56 32 625 32 2111 Cuts at: 7669 7701 7743 7775 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2111 1734 722 625 423 234 135 56 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3106 3511 4525 6372 6618 669 Size: 988 281 1168 405 1014 1847 246 3079 Fragments arranged by size: 3079 1847 1168 1014 988 405 281 246 HindIII A'AGCT_T Cuts at: 748 2014 748 Size: 1266 7762 HpaI GTT'AAC Cuts at: 6618 6618 Size: 9028 KasI G'GCGC_C Cuts at: 2108 5953 6007 6064 6224 6308 2108 Size: 3845 54 57 160 84 4828 Fragments arranged by size: 4828 3845 160 84 57 54 KpnI G_GTAC'C Cuts at: 2559 6353 2559 Size: 3794 5234 MfeI C'AATT_G Cuts at: 6627 6627 Size: 9028 MluI A'CGCG_T Cuts at: 8904 8904 Size: 9028 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2169 5236 5346 5808 8142 8326 1305 Size: 864 3067 110 462 2334 184 2007 Fragments arranged by size: 3067 2334 2007 864 462 184 110 MscI TGG'CCA Cuts at: 10 64 1489 1832 4902 10 Size: 54 1425 343 3070 4136 Fragments arranged by size: 4136 3070 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2055 3459 3465 3633 4388 4479 4916 Size: 648 889 1404 6 168 755 91 437 Cuts at: 4916 6986 7345 7504 518 Size: 2070 359 159 2042 Fragments arranged by size: 2070 2042 1404 889 755 648 437 359 168 159 91 6 MspA1I CmG'CkG Cuts at: 1606 2086 3883 5146 5248 5696 6153 6444 7121 Size: 480 1797 1263 102 448 457 291 677 Cuts at: 7121 8269 8514 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1797 1263 1148 677 480 457 448 291 245 102 NaeI GCC'GGC Cuts at: 4465 5983 6098 6123 6133 4465 Size: 1518 115 25 10 7360 Fragments arranged by size: 7360 1518 115 25 10 NarI GG'CG_CC Cuts at: 2109 5954 6008 6065 6225 6309 2109 Size: 3845 54 57 160 84 4828 Fragments arranged by size: 4828 3845 160 84 57 54 NcoI C'CATG_G Cuts at: 513 1065 4064 4389 5040 513 Size: 552 2999 325 651 4501 Fragments arranged by size: 4501 2999 651 552 325 NdeI CA'TA_TG Cuts at: 387 3058 4231 387 Size: 2671 1173 5184 Fragments arranged by size: 5184 2671 1173 NgoMIV G'CCGG_C Cuts at: 4463 5981 6096 6121 6131 4463 Size: 1518 115 25 10 7360 Fragments arranged by size: 7360 1518 115 25 10 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9028 NotI GC'GGCC_GC Cuts at: 6475 6475 Size: 9028 NsiI A_TGCA'T Cuts at: 3256 3256 Size: 9028 NspI r_CATG'y Cuts at: 1132 1229 3181 4921 5585 6386 7931 1132 Size: 97 1952 1740 664 801 1545 2229 Fragments arranged by size: 2229 1952 1740 1545 801 664 97 PasI CC'CwG_GG Cuts at: 1348 3841 1348 Size: 2493 6535 PciI A'CATG_T Cuts at: 7927 7927 Size: 9028 PflMI CCAn_nnn'nTGG Cuts at: 1108 3728 1108 Size: 2620 6408 PfoI T'CCnGG_A Cuts at: 2617 3144 2617 Size: 527 8501 PmeI GTTT'AAAC Cuts at: 6332 6332 Size: 9028 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7097 7129 8610 8642 7097 Size: 32 1481 32 7483 Fragments arranged by size: 7483 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2275 2452 5656 6194 2275 Size: 177 3204 538 5109 Fragments arranged by size: 5109 3204 538 177 PshAI GACnn'nnGTC Cuts at: 1613 2328 1613 Size: 715 8313 PsiI TTA'TAA Cuts at: 4284 6598 4284 Size: 2314 6714 PspOMI G'GGCC_C Cuts at: 6205 6257 6205 Size: 52 8976 PspXI vC'TCGA_Gb Cuts at: 5945 5945 Size: 9028 PstI C_TGCA'G Cuts at: 830 3213 3357 3388 4713 4852 6380 830 Size: 2383 144 31 1325 139 1528 3478 Fragments arranged by size: 3478 2383 1528 1325 144 139 31 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9028 PvuII CAG'CTG Cuts at: 2086 5146 2086 Size: 3060 5968 SacI G_AGCT'C Cuts at: 4184 4239 4998 6093 4184 Size: 55 759 1095 7119 Fragments arranged by size: 7119 1095 759 55 SalI G'TCGA_C Cuts at: 1936 6370 1936 Size: 4434 4594 SanDI GG'GwC_CC Cuts at: 6194 6194 Size: 9028 SbfI CC_TGCA'GG Cuts at: 3213 6380 3213 Size: 3167 5861 ScaI AGT'ACT Cuts at: 7276 8988 7276 Size: 1712 7316 SexAI A'CCwGG_T Cuts at: 3513 3513 Size: 9028 SfcI C'TryA_G Cuts at: 826 1045 3164 3209 3353 3384 3873 4709 4848 Size: 219 2119 45 144 31 489 836 139 Cuts at: 4848 5233 5302 5326 5371 6376 7511 8192 8383 Size: 385 69 24 45 1005 1135 681 191 Cuts at: 8383 826 Size: 1471 Fragments arranged by size: 2119 1471 1135 1005 836 681 489 385 219 191 144 139 69 45 45 31 24 SfoI GGC'GCC Cuts at: 2110 5955 6009 6066 6226 6310 2110 Size: 3845 54 57 160 84 4828 Fragments arranged by size: 4828 3845 160 84 57 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9028 SgrAI Cr'CCGG_yG Cuts at: 1700 6121 1700 Size: 4421 4607 SmaI CCC'GGG Cuts at: 1872 3248 6013 6111 6138 6219 6355 1872 Size: 1376 2765 98 27 81 136 4545 Fragments arranged by size: 4545 2765 1376 136 98 81 27 SmlI C'TyrA_G Cuts at: 820 1017 1943 2288 4001 5636 5945 7132 8033 Size: 197 926 345 1713 1635 309 1187 901 Cuts at: 8033 8295 820 Size: 262 1553 Fragments arranged by size: 1713 1635 1553 1187 926 901 345 309 262 197 SnaBI TAC'GTA Cuts at: 493 6819 493 Size: 6326 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9028 SphI G_CATG'C Cuts at: 1132 6386 1132 Size: 5254 3774 SrfI GCCC'GGGC Cuts at: 6013 6138 6013 Size: 125 8903 SspI AAT'ATT Cuts at: 5 52 6952 5 Size: 47 6900 2081 Fragments arranged by size: 6900 2081 47 StuI AGG'CCT Cuts at: 1517 2722 4205 4786 1517 Size: 1205 1483 581 5759 Fragments arranged by size: 5759 1483 1205 581 StyI C'CwwG_G Cuts at: 513 1065 3448 4064 4389 5040 5295 6467 513 Size: 552 2383 616 325 651 255 1172 3074 Fragments arranged by size: 3074 2383 1172 651 616 552 325 255 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3815 4107 4437 4619 5032 7055 7072 Size: 1255 2235 292 330 182 413 2023 17 Cuts at: 7072 7225 7410 7855 325 Size: 153 185 445 1498 Fragments arranged by size: 2235 2023 1498 1255 445 413 330 292 185 182 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2832 3651 3964 Size: 274 80 33 51 1443 855 819 313 Cuts at: 3964 7274 8986 96 Size: 3310 1712 138 Fragments arranged by size: 3310 1712 1443 855 819 313 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3744 3776 3870 3902 4166 4198 5607 5639 8988 Size: 32 94 32 264 32 1409 32 3349 Cuts at: 8988 9020 3744 Size: 32 3752 Fragments arranged by size: 3752 3349 1409 264 94 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9028 XbaI T'CTAG_A Cuts at: 6364 6364 Size: 9028 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3191 5814 3191 Size: 2623 6405 XhoI C'TCGA_G Cuts at: 1943 5945 1943 Size: 4002 5026 XmaI C'CCGG_G Cuts at: 1870 3246 6011 6109 6136 6217 6353 1870 Size: 1376 2765 98 27 81 136 4545 Fragments arranged by size: 4545 2765 1376 136 98 81 27 XmnI GAAnn'nnTTC Cuts at: 7157 7157 Size: 9028 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6836 276 Size: 53 83 186 936 5302 2468 Fragments arranged by size: 5302 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BsiWI BstZ17I FseI FspAI NruI PacI PmlI PsrI RsrII SacII SapI SfiI SwaI