##################################
# NO CUT ENZYMES (  206 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstF5I      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FokI        FseI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HpaII       HphI        KasI        KpnI        KspI        
MaeII       MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AciI            4  GCGG                 CCGC(-3/-1)
AcsI           74  GAATTC               [GA]^AATT[CT]
AlwI           67  GGATC                GGATC(4/5)
AlwNI          31  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI           74  GAATTC               [GA]^AATT[CT]
AspI          133  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         262  GGACC                G^G[AT]CC
BfaI          121  CTAG                 C^TAG
BpmI          251  CTGGAG               CTGGAG(16/14)
BseRI         254  GAGGAG               GAGGAG(10/8)
BsrI          150  ACTGG                ACTGG(1/-1)
BssKI         250  CCTGG                ^CC[ACGT]GG
BstAPI         30  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         250  CCTGG                CC^[AT]GG
Cfr13I        262  GGACC                G^G[ACGT]CC
DraII         261  AGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII         79  CACATCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I      261  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI          74  GAATTC               G^AATTC
EcoRII        250  CCTGG                ^CC[AT]GG
MaeI          121  CTAG                 C^TAG
MaeIII        160  GTCAC                ^GT[ACGT]AC
MnlI          254  GAGG                 CCTC(7/6)
MslI           81  CATCGTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         31  CAGCTG               C[AC]G^C[GT]G
MvaI          250  CCTGG                CC^[AT]GG
NlaIV         246  GGTTCC               GG[ACGT]^[ACGT]CC
PflFI         133  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         261  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII          31  CAGCTG               CAG^CTG
Sau96I        262  GGACC                G^G[ACGT]CC
ScrFI         250  CCTGG                CC^[ACGT]GG
Tsp45I        160  GTCAC                ^GT[GC]AC
TspRI         162  CACTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       133  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AfaI          113  GTAC                 GT^AC
              148  GTAC                
AluI           32  AGCT                 AG^CT
               47  AGCT                
DdeI           42  CTTAG                C^T[ACGT]AG
              164  CTGAG               
DpnII          68  GATC                 ^GATC
              208  GATC                
EarI          116  CTCTTC               CTCTTC(1/4)
               90  GAAGAG              
Ksp632I       116  CTCTTC               CTCTTC(1/4)
               90  GAAGAG              
MboI           68  GATC                 ^GATC
              208  GATC                
NdeII          68  GATC                 ^GATC
              208  GATC                
RsaI          113  GTAC                 GT^AC
              148  GTAC                
Sau3AI         68  GATC                 ^GATC
              208  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

BbvI        Fnu4HI      ItaI        MboII       MwoI        
TseI        Tsp509I