(Circular) (Six-base) MAPSORT of: pFN21AB6721.seq Check: 8923 from: 1 to: 4978 pFN21AB6721 4978 bp With 182 enzymes: SgfI * October 20, 2009 10:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2788 278 Size: 53 83 186 936 1252 2468 Fragments arranged by size: 2468 1252 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2321 1937 Size: 384 4594 Acc65I G'GTAC_C Cuts at: 2299 2299 Size: 4978 AclI AA'CG_TT Cuts at: 1563 3105 3478 1563 Size: 1542 373 3063 Fragments arranged by size: 3063 1542 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2292 3041 4425 4693 1632 Size: 660 749 1384 268 1917 Fragments arranged by size: 1917 1384 749 660 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4781 AflIII A'CryG_T Cuts at: 1203 3877 4854 1203 Size: 2674 977 1327 Fragments arranged by size: 2674 1327 977 AgeI A'CCGG_T Cuts at: 3809 3809 Size: 4978 AhdI GACnn_n'nnGTC Cuts at: 3707 3707 Size: 4978 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4978 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4978 AlwNI CAG_nnn'CTG Cuts at: 2041 2721 4293 2041 Size: 680 1572 2726 Fragments arranged by size: 2726 1572 680 ApaLI G'TGCA_C Cuts at: 3035 4191 3035 Size: 1156 3822 ApoI r'AATT_y Cuts at: 1493 2079 2287 2513 2665 4774 1493 Size: 586 208 226 152 2109 1697 Fragments arranged by size: 2109 1697 586 226 208 152 AseI AT'TA_AT Cuts at: 160 1032 3532 160 Size: 872 2500 1606 Fragments arranged by size: 2500 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4978 AvaI C'yCGr_G Cuts at: 1870 1943 2303 1870 Size: 73 360 4545 Fragments arranged by size: 4545 360 73 BamHI G'GATC_C Cuts at: 2308 2675 2308 Size: 367 4611 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2299 3754 618 Size: 325 217 627 512 1455 1842 Fragments arranged by size: 1842 1455 627 512 325 217 BanII G_rGCy'C Cuts at: 1391 2297 1391 Size: 906 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4007 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3169 3203 1903 Size: 34 1232 34 3678 Fragments arranged by size: 3678 1232 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2870 4080 2870 Size: 1210 3768 BclI T'GATC_A Cuts at: 1548 1548 Size: 4978 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3589 136 Size: 107 122 71 631 605 337 1580 1525 Fragments arranged by size: 1580 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 4973 4973 Size: 4978 BlpI GC'TnA_GC Cuts at: 2395 2395 Size: 4978 Bme1580I G_kGCm'C Cuts at: 1790 3039 4195 1790 Size: 1249 1156 2573 Fragments arranged by size: 2573 1249 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4978 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2709 3667 442 Size: 416 53 83 399 24 1292 958 1753 Fragments arranged by size: 1753 1292 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4978 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2277 3638 1239 Size: 1038 1361 2579 Fragments arranged by size: 2579 1361 1038 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3103 3968 4266 3103 Size: 865 298 3815 Fragments arranged by size: 3815 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3641 882 Size: 617 2142 2219 Fragments arranged by size: 2219 2142 617 BsaAI yAC'GTr Cuts at: 493 1204 2769 493 Size: 711 1565 2702 Fragments arranged by size: 2702 1565 711 BsaBI GATnn'nnATC Cuts at: 4972 4972 Size: 4978 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2785 3167 3802 275 Size: 53 83 186 936 1252 382 635 1451 Fragments arranged by size: 1451 1252 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3411 3809 4083 4230 4641 3411 Size: 398 274 147 411 3748 Fragments arranged by size: 3748 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4753 Fragments arranged by size: 4753 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2274 1186 Size: 1088 3890 BseYI C'CCAG_C Cuts at: 4181 4677 4181 Size: 496 4482 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4405 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2428 3189 4217 1540 Size: 275 186 427 761 1028 2301 Fragments arranged by size: 2301 1028 761 427 275 186 BsiHKAI G_wGCw'C Cuts at: 3039 3124 4195 3039 Size: 85 1071 3822 Fragments arranged by size: 3822 1071 85 BsmI GAATG_Cn' Cuts at: 2489 2582 2489 Size: 93 4885 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2297 3039 3124 4195 1391 Size: 399 507 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 507 399 85 BspEI T'CCGG_A Cuts at: 4641 4641 Size: 4978 BspHI T'CATG_A Cuts at: 2867 4597 2867 Size: 1730 3248 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2333 844 Size: 1489 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2865 1394 Size: 429 1042 3507 Fragments arranged by size: 3507 1042 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3473 3647 65 Size: 1183 2225 174 1396 Fragments arranged by size: 2225 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3622 3809 1700 Size: 110 1812 187 2869 Fragments arranged by size: 2869 1812 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4978 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4978 BssSI C'ACGA_G Cuts at: 3038 4050 3038 Size: 1012 3966 BstAPI GCAn_nnn'nTGC Cuts at: 794 2041 794 Size: 1247 3731 BstBI TT'CG_AA Cuts at: 3782 3782 Size: 4978 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4978 BstYI r'GATC_y Cuts at: 1972 2308 2675 3060 3077 4529 4615 4627 4973 Size: 336 367 385 17 1452 86 12 346 Cuts at: 4973 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 336 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3487 Fragments arranged by size: 3487 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4239 Fragments arranged by size: 4239 683 56 BtsI GCAGTG_nn' Cuts at: 2494 3288 3308 2494 Size: 794 20 4164 Fragments arranged by size: 4164 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4978 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4943 DraI TTT'AAA Cuts at: 2282 2282 Size: 4978 DraIII CAC_nnn'GTG Cuts at: 2089 2089 Size: 4978 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3985 809 Size: 507 2669 1802 Fragments arranged by size: 2669 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2425 3314 4943 Size: 54 1425 50 275 18 595 889 1629 Cuts at: 4943 8 Size: 43 Fragments arranged by size: 1629 1425 889 595 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2425 1537 Size: 275 613 4090 Fragments arranged by size: 4090 613 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2090 2127 2908 1353 Size: 737 37 781 3423 Fragments arranged by size: 3423 781 737 37 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3565 3939 4085 387 Size: 1064 138 1976 374 146 1280 Fragments arranged by size: 1976 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2277 2292 3041 3638 4425 4693 1239 Size: 393 645 15 749 597 787 268 1524 Fragments arranged by size: 1524 787 749 645 597 393 268 15 EcoO109I rG'GnC_Cy Cuts at: 2267 2267 Size: 4978 EcoRI G'AATT_C Cuts at: 2079 2287 2665 2079 Size: 208 378 4392 Fragments arranged by size: 4392 378 208 FspI TGC'GCA Cuts at: 3484 3484 Size: 4978 HaeII r_GCGC'y Cuts at: 1678 4125 1678 Size: 2447 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3619 3651 3693 3725 1373 Size: 32 135 32 2047 32 42 32 2626 Fragments arranged by size: 2626 2047 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2322 2568 669 Size: 988 281 384 246 3079 Fragments arranged by size: 3079 988 384 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4978 HpaI GTT'AAC Cuts at: 2568 2568 Size: 4978 KpnI G_GTAC'C Cuts at: 2303 2303 Size: 4978 MfeI C'AATT_G Cuts at: 2577 2577 Size: 4978 MluI A'CGCG_T Cuts at: 4854 4854 Size: 4978 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4092 4276 1305 Size: 2787 184 2007 Fragments arranged by size: 2787 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3156 Fragments arranged by size: 3156 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2090 2936 3295 3454 518 Size: 648 924 846 359 159 2042 Fragments arranged by size: 2042 924 846 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 2394 3071 4219 4464 1606 Size: 432 356 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 432 356 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3487 Fragments arranged by size: 3487 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 4978 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4978 NotI GC'GGCC_GC Cuts at: 2425 2425 Size: 4978 NspI r_CATG'y Cuts at: 1132 1229 2336 3881 1132 Size: 97 1107 1545 2229 Fragments arranged by size: 2229 1545 1107 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4978 PciI A'CATG_T Cuts at: 3877 3877 Size: 4978 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4978 PfoI T'CCnGG_A Cuts at: 2254 2254 Size: 4978 PmeI GTTT'AAAC Cuts at: 2282 2282 Size: 4978 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3047 3079 4560 4592 3047 Size: 32 1481 32 3433 Fragments arranged by size: 3433 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2267 2267 Size: 4978 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4978 PsiI TTA'TAA Cuts at: 2548 2548 Size: 4978 PstI C_TGCA'G Cuts at: 830 2330 830 Size: 1500 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4978 PvuII CAG'CTG Cuts at: 2038 2038 Size: 4978 SalI G'TCGA_C Cuts at: 1936 2320 1936 Size: 384 4594 SbfI CC_TGCA'GG Cuts at: 2330 2330 Size: 4978 ScaI AGT'ACT Cuts at: 3226 4938 3226 Size: 1712 3266 SfcI C'TryA_G Cuts at: 826 1045 2326 3461 4142 4333 826 Size: 219 1281 1135 681 191 1471 Fragments arranged by size: 1471 1281 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4978 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4978 SmaI CCC'GGG Cuts at: 1872 2305 1872 Size: 433 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3082 3983 4245 820 Size: 197 926 1139 901 262 1553 Fragments arranged by size: 1553 1139 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2769 493 Size: 2276 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4978 SphI G_CATG'C Cuts at: 1132 2336 1132 Size: 1204 3774 SspI AAT'ATT Cuts at: 5 52 2902 5 Size: 47 2850 2081 Fragments arranged by size: 2850 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4978 StyI C'CwwG_G Cuts at: 513 1065 2004 2417 513 Size: 552 939 413 3074 Fragments arranged by size: 3074 939 552 413 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3005 3022 3175 3360 3805 325 Size: 1255 1425 17 153 185 445 1498 Fragments arranged by size: 1498 1425 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3224 4936 96 Size: 274 80 33 51 1443 1247 1712 138 Fragments arranged by size: 1712 1443 1247 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2259 2291 4938 4970 2259 Size: 32 2647 32 2267 Fragments arranged by size: 2647 2267 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2141 1316 Size: 825 4153 XbaI T'CTAG_A Cuts at: 2314 2314 Size: 4978 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4978 XmaI C'CCGG_G Cuts at: 1870 2303 1870 Size: 433 4545 XmnI GAAnn'nnTTC Cuts at: 3107 3107 Size: 4978 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2786 276 Size: 53 83 186 936 1252 2468 Fragments arranged by size: 2468 1252 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI