(Linear) (Six-base) MAPSORT of: KIBB6721.seq  Check: 3635  from: 1  to: 273



KIBB6721.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      36     273 
   Size:         36     237
 
ApoI r'AATT_y

Cuts at:      0      74     273 
   Size:         74     199
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     272     273 
   Size:        272       1
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     269     273 
   Size:        269       4
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      36     273 
   Size:         36     237
 
DraIII CAC_nnn'GTG

Cuts at:      0      84     273 
   Size:         84     189
 
EarI CTCTTCn'nnn_

Cuts at:      0      85     122     273 
   Size:         85      37     151

  Fragments arranged by size: 

                 151      85      37
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     272     273 
   Size:        272       1
 
EcoO109I rG'GnC_Cy

Cuts at:      0     262     273 
   Size:        262      11
 
EcoRI G'AATT_C

Cuts at:      0      74     273 
   Size:         74     199
 
MslI CAynn'nnrTG

Cuts at:      0      85     273 
   Size:         85     188
 
MspA1I CmG'CkG

Cuts at:      0      33     273 
   Size:         33     240
 
PfoI T'CCnGG_A

Cuts at:      0     249     273 
   Size:        249      24
 
PpuMI rG'GwC_Cy

Cuts at:      0     262     273 
   Size:        262      11
 
PvuII CAG'CTG

Cuts at:      0      33     273 
   Size:         33     240
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     254     273 
   Size:        254      19
 
Tth111I GACn'n_nGTC

Cuts at:      0     136     273 
   Size:        136     137
 

 Enzymes that do cut:

    AlwNI     ApoI     BpmI    BseRI   BstAPI   DraIII     EarI  Eco57MI
 EcoO109I    EcoRI     MslI   MspA1I     PfoI    PpuMI    PvuII     TstI
  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI