Restriction map for
pFN21AB8450
##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AvrII BalI
BbeI BbrPI BbsI BcgI BciVI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BsiWI
BspDI BspLU11I BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI FseI HgaI Hin1I
HpaI KasI KpnI KspI MaeI
MfeI MluI MluNI MscI MunI
NarI NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
StuI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccII 6 CGCG CG^CG
AccIII 1309 TCCGGA T^CCGGA
AocI 849 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1505 AGCGCT AGC^GCT
ApaI 1445 GGGCCC GGGCC^C
AspI 712 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1898 CCCGGG C^[CT]CG[GA]G
BamHI 1260 GGATCC G^GATCC
BanI 390 GGCACC G^G[CT][GA]CC
BbvCI 795 CCTCAGC CCTCAGC(-5/-2)
BclI 308 TGATCA T^GATCA
BglII 971 AGATCT A^GATCT
BsaBI 561 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1102 GAGACC GGTCTC(1/5)
BseAI 1309 TCCGGA T^CCGGA
BsgI 340 GTGCAG GTGCAG(16/14)
BsiEI 1494 CGACCG CG[GA][CT]^CG
BsoBI 1898 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1184 TGTACA T^GTACA
BspEI 1309 TCCGGA T^CCGGA
BspHI 1073 TCATGA T^CATGA
BsrBI 930 CCGCTC CCGCTC(-3/-3)
BsrDI 54 CATTGC GCAATG(2/0)
BsrFI 465 GCCGGC [GA]^CCGG[CT]
BsrGI 1184 TGTACA T^GTACA
BstUI 6 CGCG CG^CG
Bsu36I 849 CCTAAGG CC^T[ACGT]AGG
Cfr10I 465 GCCGGC [GA]^CCGG[CT]
DraIII 1870 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1432 GAAGAG CTCTTC(1/4)
Eco47III 1505 AGCGCT AGC^GCT
Eco81I 849 CCTAAGG CC^T[ACGT]AGG
EcoNI 1008 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1582 GATATC GAT^ATC
EcoT22I 326 ATGCAT ATGCA^T
FbaI 308 TGATCA T^GATCA
HaeII 1505 AGCGCT [GA]GCGC^[CT]
HindIII 1681 AAGCTT A^AGCTT
Ksp632I 1432 GAAGAG CTCTTC(1/4)
MamI 561 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1309 TCCGGA T^CCGGA
MseI 1084 TTAA T^TAA
MvnI 6 CGCG CG^CG
NaeI 465 GCCGGC GCC^GGC
NdeI 329 CATATG CA^TATG
NgoMIV 465 GCCGGC G^CCGGC
NsiI 326 ATGCAT ATGCA^T
PflFI 712 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 627 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1073 TCATGA T^CATGA
SmaI 1898 CCCGGG CCC^GGG
SmlI 1165 CTCAAG C^T[CT][GA]AG
SspBI 1184 TGTACA T^GTACA
Tru9I 1084 TTAA T^TAA
Tth111I 712 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1898 CCCGGG C^CCGGG
XmaI 1898 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 416 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1634 CAGATCCTG
AspHI 222 GTGCTC G[AT]GC[AT]^C
1162 GTGCTC
BanII 901 GAGCCC G[GA]GC[CT]^C
1445 GGGCCC
BsiHKAI 222 GTGCTC G[AT]GC[AT]^C
1162 GTGCTC
BspMI 1205 ACCTGC ACCTGC(4/8)
1623 GCAGGT
EaeI 467 CGGCCA [CT]^GGCC[GA]
1736 TGGCCG
HincII 85 GTCAAC GT[CT]^[GA]AC
553 GTCAAC
HindII 85 GTCAAC GT[CT]^[GA]AC
553 GTCAAC
MaeII 843 ACGT A^CGT
1676 ACGT
NcoI 996 CCATGG C^CATGG
1451 CCATGG
PflMI 37 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
723 CCAACGCCTGG
PpuMI 625 GGGACCC [GA]G^G[AT]CC[CT]
1004 AGGTCCT
PstI 1749 CTGCAG CTGCA^G
1847 CTGCAG
SfcI 1749 CTGCAG C^T[GA][CT]AG
1847 CTGCAG
SphI 345 GCATGC GCATG^C
1880 GCATGC
Van91I 37 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
723 CCAACGCCTGG
XcmI 61 CCAAGCCAGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
812 CCAAGGAGAATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI Asp700I
AvaII AviII BbvI BcnI BmyI
BpmI BsaJI BsaWI BseRI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI