(Circular) (Six-base) MAPSORT of: pFN21AB8450.seq Check: 3638 from: 1 to: 6619 pFN21AB8450 6619 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3619 3619 Size: 6619 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4429 278 Size: 53 83 186 936 2893 2468 Fragments arranged by size: 2893 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3962 1937 Size: 2025 4594 Acc65I G'GTAC_C Cuts at: 3940 3940 Size: 6619 AclI AA'CG_TT Cuts at: 1563 4746 5119 1563 Size: 3183 373 3063 Fragments arranged by size: 3183 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2502 2681 3263 4682 6066 6334 1632 Size: 870 179 582 1419 1384 268 1917 Fragments arranged by size: 1917 1419 1384 870 582 268 179 AfeI AGC'GCT Cuts at: 3512 3512 Size: 6619 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6422 AflIII A'CryG_T Cuts at: 1203 5518 6495 1203 Size: 4315 977 1327 Fragments arranged by size: 4315 1327 977 AgeI A'CCGG_T Cuts at: 5450 5450 Size: 6619 AhdI GACnn_n'nnGTC Cuts at: 5348 5348 Size: 6619 AleI CACnn'nnGTG Cuts at: 1166 2540 3521 1166 Size: 1374 981 4264 Fragments arranged by size: 4264 1374 981 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3651 3863 1184 Size: 2467 212 3940 Fragments arranged by size: 3940 2467 212 AlwNI CAG_nnn'CTG Cuts at: 2426 3644 4362 5934 2426 Size: 1218 718 1572 3111 Fragments arranged by size: 3111 1572 1218 718 ApaI G_GGCC'C Cuts at: 3454 3454 Size: 6619 ApaLI G'TGCA_C Cuts at: 4676 5832 4676 Size: 1156 5463 ApoI r'AATT_y Cuts at: 1493 3928 4154 4306 6415 1493 Size: 2435 226 152 2109 1697 Fragments arranged by size: 2435 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5173 160 Size: 872 4141 1606 Fragments arranged by size: 4141 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 AvaI C'yCGr_G Cuts at: 1870 1943 3903 3944 1870 Size: 73 1960 41 4545 Fragments arranged by size: 4545 1960 73 41 BamHI G'GATC_C Cuts at: 3265 3949 4316 3265 Size: 684 367 5568 Fragments arranged by size: 5568 684 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2395 3940 5395 618 Size: 325 217 627 608 1545 1455 1842 Fragments arranged by size: 1842 1545 1455 627 608 325 217 BanII G_rGCy'C Cuts at: 1391 2910 3454 3938 1391 Size: 1519 544 484 4072 Fragments arranged by size: 4072 1519 544 484 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5648 BbvCI CC'TCA_GC Cuts at: 2801 2801 Size: 6619 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4810 4844 1903 Size: 34 2873 34 3678 Fragments arranged by size: 3678 2873 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4511 5721 4511 Size: 1210 5409 BclI T'GATC_A Cuts at: 1548 2313 1548 Size: 765 5854 BfrBI ATG'CAT Cuts at: 2333 2333 Size: 6619 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5230 136 Size: 107 122 71 631 605 3558 1525 Fragments arranged by size: 3558 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2976 6614 2976 Size: 3638 2981 BlpI GC'TnA_GC Cuts at: 4036 4036 Size: 6619 Bme1580I G_kGCm'C Cuts at: 1790 3239 3454 4680 5836 1790 Size: 1449 215 1226 1156 2573 Fragments arranged by size: 2573 1449 1226 1156 215 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6619 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2206 4350 5308 Size: 416 53 83 399 24 789 2144 958 Cuts at: 5308 442 Size: 1753 Fragments arranged by size: 2144 1753 958 789 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6619 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2431 3832 2431 Size: 1401 5218 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2053 2639 2771 2849 3782 3881 5279 1239 Size: 814 586 132 78 933 99 1398 2579 Fragments arranged by size: 2579 1398 933 814 586 132 99 78 Bpu10I CC'TnA_GC Cuts at: 2801 2801 Size: 6619 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3155 4744 5609 5907 3155 Size: 1589 865 298 3867 Fragments arranged by size: 3867 1589 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3101 5282 882 Size: 617 1602 2181 2219 Fragments arranged by size: 2219 2181 1602 617 BsaAI yAC'GTr Cuts at: 493 1204 4410 493 Size: 711 3206 2702 Fragments arranged by size: 3206 2702 711 BsaBI GATnn'nnATC Cuts at: 2570 6613 2570 Size: 4043 2576 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4426 4808 5443 275 Size: 53 83 186 936 2893 382 635 1451 Fragments arranged by size: 2893 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2118 2562 3314 5052 5450 5724 5871 6282 2118 Size: 444 752 1738 398 274 147 411 2455 Fragments arranged by size: 2455 1738 752 444 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2258 2288 2708 2738 2858 Size: 30 165 30 855 30 420 30 120 Cuts at: 2858 2888 1178 Size: 30 4909 Fragments arranged by size: 4909 855 420 165 120 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2672 2757 2857 3119 3878 1186 Size: 1486 85 100 262 759 3927 Fragments arranged by size: 3927 1486 759 262 100 85 BseYI C'CCAG_C Cuts at: 2261 2891 3835 5822 6318 2261 Size: 630 944 1987 496 2562 Fragments arranged by size: 2562 1987 944 630 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2366 1163 Size: 573 630 5416 Fragments arranged by size: 5416 630 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3502 4069 4830 5858 1540 Size: 275 186 1501 567 761 1028 2301 Fragments arranged by size: 2301 1501 1028 761 567 275 186 BsiHKAI G_wGCw'C Cuts at: 2231 3171 4680 4765 5836 2231 Size: 940 1509 85 1071 3014 Fragments arranged by size: 3014 1509 1071 940 85 BsmI GAATG_Cn' Cuts at: 2135 2785 3782 4130 4223 2135 Size: 650 997 348 93 4531 Fragments arranged by size: 4531 997 650 348 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2728 2839 3572 2728 Size: 111 733 5775 Fragments arranged by size: 5775 733 111 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2231 2910 3171 3239 3454 3938 4680 Size: 399 441 679 261 68 215 484 742 Cuts at: 4680 4765 5836 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 679 484 441 399 261 215 85 68 BspEI T'CCGG_A Cuts at: 3314 6282 3314 Size: 2968 3651 BspHI T'CATG_A Cuts at: 3078 4508 6238 3078 Size: 1430 1730 3459 Fragments arranged by size: 3459 1730 1430 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3219 3619 3974 844 Size: 2375 400 355 3489 Fragments arranged by size: 3489 2375 400 355 BsrBI CCG'CTC Cuts at: 1394 1823 2937 4506 1394 Size: 429 1114 1569 3507 Fragments arranged by size: 3507 1569 1114 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2058 5114 5288 65 Size: 1183 810 3056 174 1396 Fragments arranged by size: 3056 1396 1183 810 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2470 5263 5450 1700 Size: 110 660 2793 187 2869 Fragments arranged by size: 2869 2793 660 187 110 BsrGI T'GTAC_A Cuts at: 96 3189 96 Size: 3093 3526 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6619 BssSI C'ACGA_G Cuts at: 4679 5691 4679 Size: 1012 5607 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6619 BstBI TT'CG_AA Cuts at: 5423 5423 Size: 6619 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6619 BstYI r'GATC_y Cuts at: 1972 2976 3265 3355 3640 3949 4316 4701 4718 Size: 1004 289 90 285 309 367 385 17 Cuts at: 4718 6170 6256 6268 6614 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1004 385 367 346 309 289 285 90 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2855 2855 Size: 6619 BtgI C'CryG_G Cuts at: 513 1065 2508 3001 3060 3456 513 Size: 552 1443 493 59 396 3676 Fragments arranged by size: 3676 1443 552 493 396 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5880 Fragments arranged by size: 5880 683 56 BtsI GCAGTG_nn' Cuts at: 4135 4929 4949 4135 Size: 794 20 5805 Fragments arranged by size: 5805 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6619 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6584 DraI TTT'AAA Cuts at: 3923 3923 Size: 6619 DraIII CAC_nnn'GTG Cuts at: 3880 3880 Size: 6619 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5626 809 Size: 507 4310 1802 Fragments arranged by size: 4310 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2472 3741 4066 Size: 54 1425 50 275 18 642 1269 325 Cuts at: 4066 4955 6584 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1269 889 642 325 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4066 1537 Size: 275 2254 4090 Fragments arranged by size: 4090 2254 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3432 4549 1353 Size: 2079 1117 3423 Fragments arranged by size: 3423 2079 1117 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5206 5580 5726 387 Size: 1064 138 3617 374 146 1280 Fragments arranged by size: 3617 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2053 2502 2639 2681 2771 2849 3263 Size: 393 421 449 137 42 90 78 414 Cuts at: 3263 3782 3881 4682 5279 6066 6334 1239 Size: 519 99 801 597 787 268 1524 Fragments arranged by size: 1524 801 787 597 519 449 421 414 393 268 137 99 90 78 42 EcoNI CCTnn'n_nnAGG Cuts at: 3017 3017 Size: 6619 EcoO109I rG'GnC_Cy Cuts at: 2246 2631 3010 3450 3451 3811 2246 Size: 385 379 440 1 360 5054 Fragments arranged by size: 5054 440 385 379 360 1 EcoRI G'AATT_C Cuts at: 3928 4306 3928 Size: 378 6241 EcoRV GAT'ATC Cuts at: 3589 3589 Size: 6619 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3043 3043 Size: 6619 FspI TGC'GCA Cuts at: 3146 3257 3491 5125 3146 Size: 111 234 1634 4640 Fragments arranged by size: 4640 1634 234 111 HaeII r_GCGC'y Cuts at: 1678 3514 5766 1678 Size: 1836 2252 2531 Fragments arranged by size: 2531 2252 1836 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2708 2740 3347 3379 3578 Size: 32 135 32 1136 32 607 32 199 Cuts at: 3578 3610 5260 5292 5334 5366 1373 Size: 32 1650 32 42 32 2626 Fragments arranged by size: 2626 1650 1136 607 199 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2092 2560 3963 4209 669 Size: 988 281 154 468 1403 246 3079 Fragments arranged by size: 3079 1403 988 468 281 246 154 HindIII A'AGCT_T Cuts at: 748 3686 748 Size: 2938 3681 HpaI GTT'AAC Cuts at: 4209 4209 Size: 6619 KpnI G_GTAC'C Cuts at: 3944 3944 Size: 6619 MfeI C'AATT_G Cuts at: 4218 4218 Size: 6619 MluI A'CGCG_T Cuts at: 6495 6495 Size: 6619 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2550 3366 3849 5733 5917 1305 Size: 1245 816 483 1884 184 2007 Fragments arranged by size: 2007 1884 1245 816 483 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4797 Fragments arranged by size: 4797 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2465 2540 3162 3182 3521 4577 4936 Size: 648 1299 75 622 20 339 1056 359 Cuts at: 4936 5095 518 Size: 159 2042 Fragments arranged by size: 2042 1299 1056 648 622 359 339 159 75 20 MspA1I CmG'CkG Cuts at: 1606 2440 2701 2743 3739 3847 4035 4712 5860 Size: 834 261 42 996 108 188 677 1148 Cuts at: 5860 6105 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 996 834 677 261 245 188 108 42 NaeI GCC'GGC Cuts at: 2472 2472 Size: 6619 NcoI C'CATG_G Cuts at: 513 1065 3001 3456 513 Size: 552 1936 455 3676 Fragments arranged by size: 3676 1936 552 455 NdeI CA'TA_TG Cuts at: 387 2335 387 Size: 1948 4671 NgoMIV G'CCGG_C Cuts at: 2470 2470 Size: 6619 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6619 NotI GC'GGCC_GC Cuts at: 4066 4066 Size: 6619 NsiI A_TGCA'T Cuts at: 2335 2335 Size: 6619 NspI r_CATG'y Cuts at: 1132 1229 2354 2776 3889 3977 5522 1132 Size: 97 1125 422 1113 88 1545 2229 Fragments arranged by size: 2229 1545 1125 1113 422 97 88 PasI CC'CwG_GG Cuts at: 1348 2603 1348 Size: 1255 5364 PciI A'CATG_T Cuts at: 5518 5518 Size: 6619 PflMI CCAn_nnn'nTGG Cuts at: 1108 2048 2734 1108 Size: 940 686 4993 Fragments arranged by size: 4993 940 686 PfoI T'CCnGG_A Cuts at: 2616 2616 Size: 6619 PmeI GTTT'AAAC Cuts at: 3923 3923 Size: 6619 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2833 2865 4688 4720 6201 6233 2833 Size: 32 1823 32 1481 32 3219 Fragments arranged by size: 3219 1823 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2631 3010 2631 Size: 379 6240 PshAI GACnn'nnGTC Cuts at: 1613 2636 1613 Size: 1023 5596 PsiI TTA'TAA Cuts at: 4189 4189 Size: 6619 PspOMI G'GGCC_C Cuts at: 3450 3450 Size: 6619 PstI C_TGCA'G Cuts at: 830 3758 3856 3971 830 Size: 2928 98 115 3478 Fragments arranged by size: 3478 2928 115 98 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6619 PvuII CAG'CTG Cuts at: 2440 2701 2743 2440 Size: 261 42 6316 Fragments arranged by size: 6316 261 42 SalI G'TCGA_C Cuts at: 1936 3961 1936 Size: 2025 4594 SanDI GG'GwC_CC Cuts at: 2631 2631 Size: 6619 SbfI CC_TGCA'GG Cuts at: 3971 3971 Size: 6619 ScaI AGT'ACT Cuts at: 4867 6579 4867 Size: 1712 4907 SfcI C'TryA_G Cuts at: 826 1045 3754 3852 3967 5102 5783 5974 826 Size: 219 2709 98 115 1135 681 191 1471 Fragments arranged by size: 2709 1471 1135 681 219 191 115 98 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6619 SmaI CCC'GGG Cuts at: 1872 3905 3946 1872 Size: 2033 41 4545 Fragments arranged by size: 4545 2033 41 SmlI C'TyrA_G Cuts at: 820 1017 1943 3170 4723 5624 5886 820 Size: 197 926 1227 1553 901 262 1553 Fragments arranged by size: 1553 1553 1227 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4410 493 Size: 3917 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6619 SphI G_CATG'C Cuts at: 1132 2354 3889 3977 1132 Size: 1222 1535 88 3774 Fragments arranged by size: 3774 1535 1222 88 SspI AAT'ATT Cuts at: 5 52 4543 5 Size: 47 4491 2081 Fragments arranged by size: 4491 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6619 StyI C'CwwG_G Cuts at: 513 1065 2187 2817 3001 3456 4058 513 Size: 552 1122 630 184 455 602 3074 Fragments arranged by size: 3074 1122 630 602 552 455 184 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2214 2295 2413 4646 4663 4816 5001 Size: 1255 634 81 118 2233 17 153 185 Cuts at: 5001 5446 325 Size: 445 1498 Fragments arranged by size: 2233 1498 1255 634 445 185 153 118 81 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3189 3222 4865 Size: 274 80 33 51 1443 1212 33 1643 Cuts at: 4865 6577 96 Size: 1712 138 Fragments arranged by size: 1712 1643 1443 1212 274 138 80 51 33 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3703 3735 6579 6611 3703 Size: 32 2844 32 3711 Fragments arranged by size: 3711 2844 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2720 1316 Size: 1404 5215 XbaI T'CTAG_A Cuts at: 3955 3955 Size: 6619 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2073 2824 2011 Size: 62 751 5806 Fragments arranged by size: 5806 751 62 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6619 XmaI C'CCGG_G Cuts at: 1870 3903 3944 1870 Size: 2033 41 4545 Fragments arranged by size: 4545 2033 41 XmnI GAAnn'nnTTC Cuts at: 2946 3023 3689 4748 2946 Size: 77 666 1059 4817 Fragments arranged by size: 4817 1059 666 77 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4427 276 Size: 53 83 186 936 2893 2468 Fragments arranged by size: 2893 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BsiWI BstXI BstZ17I EcoICRI FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI