Restriction map for
pFN21AB8450
(Linear) (Six-base) MAPSORT of: KIBB8450.seq Check: 9578 from: 1 to: 1914
KIBB8450.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1614 1914
Size: 1614 300
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 676 1258 1914
Size: 497 179 582 656
Fragments arranged by size:
656 582 497 179
AfeI AGC'GCT
Cuts at: 0 1507 1914
Size: 1507 407
AleI CACnn'nnGTG
Cuts at: 0 535 1516 1914
Size: 535 981 398
Fragments arranged by size:
981 535 398
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1646 1858 1914
Size: 1646 212 56
Fragments arranged by size:
1646 212 56
AlwNI CAG_nnn'CTG
Cuts at: 0 421 1639 1914
Size: 421 1218 275
Fragments arranged by size:
1218 421 275
ApaI G_GGCC'C
Cuts at: 0 1449 1914
Size: 1449 465
AvaI C'yCGr_G
Cuts at: 0 1898 1914
Size: 1898 16
BamHI G'GATC_C
Cuts at: 0 1260 1914
Size: 1260 654
BanI G'GyrC_C
Cuts at: 0 390 1914
Size: 390 1524
BanII G_rGCy'C
Cuts at: 0 905 1449 1914
Size: 905 544 465
Fragments arranged by size:
905 544 465
BbvCI CC'TCA_GC
Cuts at: 0 796 1914
Size: 796 1118
BclI T'GATC_A
Cuts at: 0 308 1914
Size: 308 1606
BfrBI ATG'CAT
Cuts at: 0 328 1914
Size: 328 1586
BglII A'GATC_T
Cuts at: 0 971 1914
Size: 971 943
Bme1580I G_kGCm'C
Cuts at: 0 1234 1449 1914
Size: 1234 215 465
Fragments arranged by size:
1234 465 215
BmrI ACTGGGnnnn_n'
Cuts at: 0 201 1914
Size: 201 1713
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 426 1827 1914
Size: 426 1401 87
Fragments arranged by size:
1401 426 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 634 766 844 1777 1876 1914
Size: 48 586 132 78 933 99 38
Fragments arranged by size:
933 586 132 99 78 48 38
Bpu10I CC'TnA_GC
Cuts at: 0 796 1914
Size: 796 1118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1150 1914
Size: 1150 764
BsaI GGTCTCn'nnnn_
Cuts at: 0 1096 1914
Size: 1096 818
BsaBI GATnn'nnATC
Cuts at: 0 565 1914
Size: 565 1349
BsaWI w'CCGG_w
Cuts at: 0 113 557 1309 1914
Size: 113 444 752 605
Fragments arranged by size:
752 605 444 113
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 253 283 703 733 853 883 1914
Size: 253 30 420 30 120 30 1031
Fragments arranged by size:
1031 420 253 120 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 667 752 852 1114 1873 1914
Size: 667 85 100 262 759 41
Fragments arranged by size:
759 667 262 100 85 41
BseYI C'CCAG_C
Cuts at: 0 256 886 1830 1914
Size: 256 630 944 84
Fragments arranged by size:
944 630 256 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 1914
Size: 361 1553
BsiEI CG_ry'CG
Cuts at: 0 1497 1914
Size: 1497 417
BsiHKAI G_wGCw'C
Cuts at: 0 226 1166 1914
Size: 226 940 748
Fragments arranged by size:
940 748 226
BsmI GAATG_Cn'
Cuts at: 0 130 780 1777 1914
Size: 130 650 997 137
Fragments arranged by size:
997 650 137 130
BsmBI CGTCTCn'nnnn_
Cuts at: 0 723 834 1567 1914
Size: 723 111 733 347
Fragments arranged by size:
733 723 347 111
Bsp1286I G_dGCh'C
Cuts at: 0 226 905 1166 1234 1449 1914
Size: 226 679 261 68 215 465
Fragments arranged by size:
679 465 261 226 215 68
BspEI T'CCGG_A
Cuts at: 0 1309 1914
Size: 1309 605
BspHI T'CATG_A
Cuts at: 0 1073 1914
Size: 1073 841
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1214 1614 1914
Size: 1214 400 300
Fragments arranged by size:
1214 400 300
BsrBI CCG'CTC
Cuts at: 0 932 1914
Size: 932 982
BsrDI GCAATG_nn'
Cuts at: 0 53 1914
Size: 53 1861
BsrFI r'CCGG_y
Cuts at: 0 465 1914
Size: 465 1449
BsrGI T'GTAC_A
Cuts at: 0 1184 1914
Size: 1184 730
BstYI r'GATC_y
Cuts at: 0 971 1260 1350 1635 1914
Size: 971 289 90 285 279
Fragments arranged by size:
971 289 285 279 90
Bsu36I CC'TnA_GG
Cuts at: 0 850 1914
Size: 850 1064
BtgI C'CryG_G
Cuts at: 0 503 996 1055 1451 1914
Size: 503 493 59 396 463
Fragments arranged by size:
503 493 463 396 59
DraIII CAC_nnn'GTG
Cuts at: 0 1875 1914
Size: 1875 39
EaeI y'GGCC_r
Cuts at: 0 467 1736 1914
Size: 467 1269 178
Fragments arranged by size:
1269 467 178
EarI CTCTTCn'nnn_
Cuts at: 0 1427 1914
Size: 1427 487
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 497 634 676 766 844 1258 1777
Size: 48 449 137 42 90 78 414 519
Cuts at: 1777 1876 1914
Size: 99 38
Fragments arranged by size:
519 449 414 137 99 90 78 48
42 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1012 1914
Size: 1012 902
EcoO109I rG'GnC_Cy
Cuts at: 0 241 626 1005 1445 1446 1806 1914
Size: 241 385 379 440 1 360 108
Fragments arranged by size:
440 385 379 360 241 108 1
EcoRV GAT'ATC
Cuts at: 0 1584 1914
Size: 1584 330
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1038 1914
Size: 1038 876
FspI TGC'GCA
Cuts at: 0 1141 1252 1486 1914
Size: 1141 111 234 428
Fragments arranged by size:
1141 428 234 111
HaeII r_GCGC'y
Cuts at: 0 1509 1914
Size: 1509 405
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 703 735 1342 1374 1573 1605 1914
Size: 703 32 607 32 199 32 309
Fragments arranged by size:
703 607 309 199 32 32 32
HincII GTy'rAC
Cuts at: 0 87 555 1914
Size: 87 468 1359
Fragments arranged by size:
1359 468 87
HindIII A'AGCT_T
Cuts at: 0 1681 1914
Size: 1681 233
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 1361 1844 1914
Size: 545 816 483 70
Fragments arranged by size:
816 545 483 70
MslI CAynn'nnrTG
Cuts at: 0 460 535 1157 1177 1516 1914
Size: 460 75 622 20 339 398
Fragments arranged by size:
622 460 398 339 75 20
MspA1I CmG'CkG
Cuts at: 0 435 696 738 1734 1842 1914
Size: 435 261 42 996 108 72
Fragments arranged by size:
996 435 261 108 72 42
NaeI GCC'GGC
Cuts at: 0 467 1914
Size: 467 1447
NcoI C'CATG_G
Cuts at: 0 996 1451 1914
Size: 996 455 463
Fragments arranged by size:
996 463 455
NdeI CA'TA_TG
Cuts at: 0 330 1914
Size: 330 1584
NgoMIV G'CCGG_C
Cuts at: 0 465 1914
Size: 465 1449
NsiI A_TGCA'T
Cuts at: 0 330 1914
Size: 330 1584
NspI r_CATG'y
Cuts at: 0 349 771 1884 1914
Size: 349 422 1113 30
Fragments arranged by size:
1113 422 349 30
PasI CC'CwG_GG
Cuts at: 0 598 1914
Size: 598 1316
PflMI CCAn_nnn'nTGG
Cuts at: 0 43 729 1914
Size: 43 686 1185
Fragments arranged by size:
1185 686 43
PfoI T'CCnGG_A
Cuts at: 0 611 1914
Size: 611 1303
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 828 860 1914
Size: 828 32 1054
Fragments arranged by size:
1054 828 32
PpuMI rG'GwC_Cy
Cuts at: 0 626 1005 1914
Size: 626 379 909
Fragments arranged by size:
909 626 379
PshAI GACnn'nnGTC
Cuts at: 0 631 1914
Size: 631 1283
PspOMI G'GGCC_C
Cuts at: 0 1445 1914
Size: 1445 469
PstI C_TGCA'G
Cuts at: 0 1753 1851 1914
Size: 1753 98 63
Fragments arranged by size:
1753 98 63
PvuII CAG'CTG
Cuts at: 0 435 696 738 1914
Size: 435 261 42 1176
Fragments arranged by size:
1176 435 261 42
SanDI GG'GwC_CC
Cuts at: 0 626 1914
Size: 626 1288
SfcI C'TryA_G
Cuts at: 0 1749 1847 1914
Size: 1749 98 67
Fragments arranged by size:
1749 98 67
SmaI CCC'GGG
Cuts at: 0 1900 1914
Size: 1900 14
SmlI C'TyrA_G
Cuts at: 0 1165 1914
Size: 1165 749
SphI G_CATG'C
Cuts at: 0 349 1884 1914
Size: 349 1535 30
Fragments arranged by size:
1535 349 30
StyI C'CwwG_G
Cuts at: 0 182 812 996 1451 1914
Size: 182 630 184 455 463
Fragments arranged by size:
630 463 455 184 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 209 290 408 1914
Size: 209 81 118 1506
Fragments arranged by size:
1506 209 118 81
TatI w'GTAC_w
Cuts at: 0 1184 1217 1914
Size: 1184 33 697
Fragments arranged by size:
1184 697 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1698 1730 1914
Size: 1698 32 184
Fragments arranged by size:
1698 184 32
Tth111I GACn'n_nGTC
Cuts at: 0 715 1914
Size: 715 1199
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 68 819 1914
Size: 68 751 1095
Fragments arranged by size:
1095 751 68
XmaI C'CCGG_G
Cuts at: 0 1898 1914
Size: 1898 16
XmnI GAAnn'nnTTC
Cuts at: 0 941 1018 1684 1914
Size: 941 77 666 230
Fragments arranged by size:
941 666 230 77
Enzymes that do cut:
AarI AcuI AfeI AleI AlfI AlwNI ApaI AvaI
BamHI BanI BanII BbvCI BclI BfrBI BglII Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BstYI Bsu36I BtgI DraIII EaeI EarI Eco57MI EcoNI
EcoO109I EcoRV FalI FspI HaeII Hin4I HincII HindIII
MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI
NspI PasI PflMI PfoI PpiI PpuMI PshAI PspOMI
PstI PvuII SanDI SfcI SmaI SmlI SphI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BcgI BciVI BglI BlpI BmgBI BmtI
BsaAI BsaHI BsiWI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI DrdI
EagI EciI EcoICRI EcoRI FseI FspAI HpaI KasI
KpnI MfeI MluI MscI NarI NheI NotI NruI
PacI PciI PmeI PmlI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
StuI SwaI XbaI XhoI ZraI