Restriction map for
pFN21AB7316
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI Asp718I
AspEI AvaI AviII AvrII BamHI
BanI BbeI BcgI BclI BfrI
BglI BlnI BsaHI BsaWI BseAI
BsiWI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CfoI
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PvuI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I SspBI StuI
SwaI Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AclI 391 AACGTT AA^CGTT
AocI 3088 CCTCAGG CC^T[ACGT]AGG
Asp700I 2233 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1966 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 846 TGGCCA TGG^CCA
BbrPI 1091 CACGTG CAC^GTG
BbvCI 1768 CCTCAGC CCTCAGC(-5/-2)
BcnI 2460 CCCGG CC^[GC]GG
BfaI 3134 CTAG C^TAG
BglII 419 AGATCT A^GATCT
BlpI 2788 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2788 GCTGAGC GC^T[ACGT]AGC
BsaBI 1253 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 691 GGTCTC GGTCTC(1/5)
BsgI 2906 CTGCAC GTGCAG(16/14)
BsiEI 1996 CGGTCG CG[GA][CT]^CG
BsmBI 1991 CGTCTC CGTCTC(1/5)
BsmI 1230 GAATGC GAATGC(1/-1)
BspHI 150 TCATGA T^CATGA
BsrBI 498 CCGCTC CCGCTC(-3/-3)
BstXI 870 CCATCTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3088 CCTCAGG CC^T[ACGT]AGG
CelII 2788 GCTGAGC GC^T[ACGT]AGC
DraI 1035 TTTAAA TTT^AAA
DraIII 311 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 222 CCGTGG C^C[GA][CT]GG
EaeI 846 TGGCCA [CT]^GGCC[GA]
Eco81I 3088 CCTCAGG CC^T[ACGT]AGG
EcoNI 2983 CCTGTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 629 CCTTGG C^C[AT][AT]GG
FauI 3076 GCGGG CCCGC(4/6)
HapII 2461 CCGG C^CGG
HgaI 2793 GCGTC GACGC(5/10)
HpaII 2461 CCGG C^CGG
MaeI 3134 CTAG C^TAG
MamI 1253 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 846 TGGCCA TGG^CCA
MscI 846 TGGCCA TGG^CCA
MspI 2461 CCGG C^CGG
NciI 2460 CCCGG CC^[GC]GG
PflFI 1966 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1091 CACGTG CAC^GTG
PmlI 1091 CACGTG CAC^GTG
PpuMI 256 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 391 AACGTT AA^CGTT
PvuII 1340 CAGCTG CAG^CTG
RcaI 150 TCATGA T^CATGA
SacI 547 GAGCTC GAGCT^C
StyI 629 CCTTGG C^C[AT][AT]GG
Tth111I 1966 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 641 CCATTCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2233 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
ApaI 1086 GGGCCC GGGCC^C
2457 GGGCCC
AspHI 547 GAGCTC G[AT]GC[AT]^C
2678 GTGCTC
AvaII 257 GGTCC G^G[AT]CC
3254 GGTCC
BbvI 1434 GCAGC GCAGC(8/12)
3049 GCTGC
BsaJI 222 CCGTGG C^C[ACGT][ACGT]GG
629 CCTTGG
BsiHKAI 547 GAGCTC G[AT]GC[AT]^C
2678 GTGCTC
BstYI 419 AGATCT [GA]^GATC[CT]
3136 AGATCC
Cac8I 2433 GCGAGC GC[ACGT]^[ACGT]GC
2995 GCAGGC
DraII 256 AGGTCCC [GA]G^G[ACGT]CC[CT]
1611 GGGGCCT
EcoO109I 256 AGGTCCC [GA]G^G[ACGT]CC[CT]
1611 GGGGCCT
EcoRV 989 GATATC GAT^ATC
2123 GATATC
MflI 419 AGATCT [GA]^GATC[CT]
3136 AGATCC
PstI 2207 CTGCAG CTGCA^G
3050 CTGCAG
SexAI 2076 ACCAGGT A^CC[AT]GGT
2423 ACCTGGT
SmlI 767 CTCAAG C^T[CT][GA]AG
2948 CTCAAG
SnaBI 13 TACGTA TAC^GTA
530 TACGTA
SspI 907 AATATT AAT^ATT
2359 AATATT
TseI 1434 GCAGC G^C[AT]GC
3049 GCTGC
XhoII 419 AGATCT [GA]^GATC[CT]
3136 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI BanII
BbsI BciVI BmyI BpmI BpuAI
BsaAI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I Tsp45I Tsp509I TspRI
TthHB8I